Rmu_sc0000487.1_g000056

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000487.1
OG0019220
Physical Location & Seq
Reverse (-)
224417 .. 230558
6142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000487.1_g000056.1.cds

Sequence Viewer

Length: 1293 bp
atgacggaggtggcacagtggcattttgctgtccttgctgtcgagtttcgtttctccgttttactttggcacaagggttttcaaggccttgcatggtggggtgtttgtgcggcgatctgcgattctgcggcaatcccaactagtcaacgacgccgtcaatcaccccaaatccggcggctaccacggcctcttctcttagaccgaggttcgagtgcaagctattcaggggcttccccttttctgcaaggataccctgaacacatctccagtcttgtagacattcttgtggaactcctggaagttgtactgttctcattcacttcgattttatgctcatccgaagagcttgaatataaattcttactcagagtacatgttatcaatgccctgcctcagaattcgaagcctgcgaatatagaatgcaagtcgacagatacatatattggcgaacgcaacctgaatgttggtgaagacttcacatggaatgccaaggaaagctctctgtattactgtcgagcattctggggtagattttttgcatcctggcatgcagttcagcctaagcgagatgatggtaaagcagcagtgttttggctggtgaacgaacatgggttctttcttagctgggataattccacctgggaccatgcagcttggtgcatgaatacctgtactgctggactcggtgtgattgtgcgtgactgccaaggaaaattcctagcagcagaggctaaaactgatcgttgcagttctgccttgatcgctgaagctaaagcagtgattgcgggtcttgacttgggtcagcagctccaaataccttcatttgtcgccttggaggacgcctttcacgcctttcgcgccttggaggacgcctcaccagcatcatttcgcatcagaccggaattctcatctccagatcgcttctcatcaaaccggaattccacgggatcaccttccttccaggctcagatcacgccaagttcagacctgttcgtcacatcccaggtcgtcaccgtcgtccacagtctgcctggctttgtccttcgtcgcccgcactccggctccgtcgcccagccccgctttgctccatctctgatagggttgtctcttctccgtcagttcgatcggcttcgtctttgtcggcgctctccgttcagaccggtgtcatctcttctccagtcacctacttcagtcggcatccttcctaacccggctcactgttccaaccctctcactcagtcaactcgccttacccaactcagccacactcgtgggataacagtcaacccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

430

Amino Acids

47.82

Weight (kDa)

8.78

Isoelectric Point (pI)

56.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g05650
rosa_chinensis RchiOBHm_Chr4g0396831
rosa_multiflora Rmu_sc0000487.1_g000056
rosa_roxburghii Rroxscaffold_5G00341670
rosa_samantha Rh4AG074800 Rh4BG070000 Rh4DG067800
rosa_wichuraiana Rw4G005920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 992
AccI GTMKAC 2 cut(s) 276, 428
AccII CGCG 1 cut(s) 858
AciI CCGC 6 cut(s) 110, 128, 175, 785, 1052, 1078
AclWI GGATC 1 cut(s) 955
AcsI RAATTY 5 cut(s) 356, 397, 713, 902, 937
AcuI CTGAAG 2 cut(s) 786, 1173
AcyI GRCGYC 3 cut(s) 151, 840, 870
AfaI GTAC 3 cut(s) 306, 372, 673
AfiI CCNNNNNNNGG 2 cut(s) 171, 1058
AflIII ACRYGT 1 cut(s) 373
AgeI ACCGGT 1 cut(s) 1159
AgsI TTSAA 2 cut(s) 83, 350
AhlI ACTAGT 1 cut(s) 140
AjnI CCWGG 6 cut(s) 294, 542, 638, 960, 1002, 1030
AleI CACNNNNGTG 1 cut(s) 1269
AluBI AGCT 7 cut(s) 219, 346, 498, 624, 653, 770, 808
AluI AGCT 7 cut(s) 219, 346, 498, 624, 653, 770, 808
Alw26I GTCTC 1 cut(s) 1110
AlwI GGATC 1 cut(s) 955
AoxI GGCC 2 cut(s) 85, 185
ApeKI GCWGC 4 cut(s) 581, 650, 722, 805
ApoI RAATTY 5 cut(s) 356, 397, 713, 902, 937
AsiGI ACCGGT 1 cut(s) 1159
AspLEI GCGC 2 cut(s) 860, 1146
AspS9I GGNCC 1 cut(s) 643
AsuC2I CCSGG 1 cut(s) 1211
AsuHPI GGTGA 7 cut(s) 153, 479, 610, 867, 942, 1003, 1173
AsuII TTCGAA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 643
BauI CACGAG 1 cut(s) 1269
BbsI GAAGAC 1 cut(s) 477
BbvI GCAGC 4 cut(s) 593, 662, 734, 817
BccI CCATC 2 cut(s) 566, 1096
BceAI ACGGC 2 cut(s) 138, 200
BciT130I CCWGG 6 cut(s) 296, 544, 640, 962, 1004, 1032
BciVI GTATCC 1 cut(s) 242
BcnI CCSGG 1 cut(s) 1211
BcoDI GTCTC 1 cut(s) 1110
BcuI ACTAGT 1 cut(s) 140
BfaI CTAG 2 cut(s) 141, 719
BfoI RGCGCY 1 cut(s) 1147
BfuI GTATCC 1 cut(s) 242
BisI GCNGC 7 cut(s) 111, 129, 176, 582, 651, 723, 806
BlsI GCNGC 7 cut(s) 112, 130, 177, 583, 652, 724, 807
Bme1390I CCNGG 7 cut(s) 296, 544, 640, 962, 1004, 1032, 1211
Bme18I GGWCC 1 cut(s) 643
BmgT120I GGNCC 1 cut(s) 643
BmiI GGNNCC 2 cut(s) 644, 1063
BmrFI CCNGG 7 cut(s) 296, 544, 640, 962, 1004, 1032, 1211
BmsI GCATC 4 cut(s) 548, 890, 900, 1206
BoxI GACNNNNGTC 2 cut(s) 798, 1162
BpiI GAAGAC 1 cut(s) 477
BplI GAGNNNNNCTC 2 cut(s) 857, 889
BpmI CTGGAG 3 cut(s) 250, 897, 1160
Bpu10I CCTNAGC 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 1211
BsaHI GRCGYC 3 cut(s) 151, 840, 870
BsaJI CCNNGG 9 cut(s) 182, 202, 489, 639, 706, 831, 861, 942, 1002
BsaWI WCCGGW 3 cut(s) 898, 933, 1159
BsaXI ACNNNNNCTCC 2 cut(s) 1046, 1076
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 1058
Bse118I RCCGGY 1 cut(s) 1159
Bse1I ACTGG 2 cut(s) 267, 1177
BseBI CCWGG 6 cut(s) 296, 544, 640, 962, 1004, 1032
BseDI CCNNGG 9 cut(s) 182, 202, 489, 639, 706, 831, 861, 942, 1002
BseGI GGATG 4 cut(s) 335, 539, 998, 1197
BseLI CCNNNNNNNGG 2 cut(s) 171, 1058
BseMII CTCAG 5 cut(s) 379, 407, 980, 1250, 1273
BseNI ACTGG 2 cut(s) 267, 1177
BseXI GCAGC 4 cut(s) 593, 662, 734, 817
BseYI CCCAGC 2 cut(s) 624, 1071
Bsh1236I CGCG 1 cut(s) 858
Bsh1285I CGRYCG 1 cut(s) 1126
BshFI GGCC 2 cut(s) 87, 187
BshTI ACCGGT 1 cut(s) 1159
BsiEI CGRYCG 1 cut(s) 1126
BsiSI CCGG 6 cut(s) 172, 899, 934, 1059, 1160, 1211
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 2 cut(s) 171, 1058
BsmAI GTCTC 1 cut(s) 1110
BsmFI GGGAC 1 cut(s) 656
BsmI GAATGC 3 cut(s) 425, 490, 518
BsnI GGCC 2 cut(s) 87, 187
Bsp119I TTCGAA 1 cut(s) 401
Bsp143I GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
BspACI CCGC 6 cut(s) 110, 128, 175, 785, 1052, 1078
BspANI GGCC 2 cut(s) 87, 187
BspCNI CTCAG 5 cut(s) 378, 406, 979, 1249, 1272
BspFNI CGCG 1 cut(s) 858
BspLI GGNNCC 2 cut(s) 644, 1063
BspPI GGATC 1 cut(s) 955
BspQI GCTCTTC 1 cut(s) 336
BspT104I TTCGAA 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 1159
BsrI ACTGG 2 cut(s) 267, 1177
BssAI RCCGGY 1 cut(s) 1159
BssECI CCNNGG 9 cut(s) 182, 202, 489, 639, 706, 831, 861, 942, 1002
BssMI GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
BssNI GRCGYC 3 cut(s) 151, 840, 870
BssSI CACGAG 1 cut(s) 1269
BssT1I CCWWGG 4 cut(s) 489, 706, 831, 861
Bst2BI CACGAG 1 cut(s) 1269
Bst2UI CCWGG 6 cut(s) 296, 544, 640, 962, 1004, 1032
Bst4CI ACNGT 7 cut(s) 18, 309, 512, 1015, 1025, 1220, 1282
Bst6I CTCTTC 4 cut(s) 195, 336, 1113, 1176
BstACI GRCGYC 3 cut(s) 151, 840, 870
BstAPI GCANNNNNTGC 1 cut(s) 782
BstBI TTCGAA 1 cut(s) 401
BstC8I GCNNGC 4 cut(s) 217, 408, 549, 1052
BstDEI CTNAG 8 cut(s) 196, 365, 393, 561, 620, 966, 1236, 1259
BstDSI CCRYGG 2 cut(s) 182, 942
BstF5I GGATG 4 cut(s) 335, 539, 998, 1197
BstFNI CGCG 1 cut(s) 858
BstH2I RGCGCY 1 cut(s) 1147
BstHHI GCGC 2 cut(s) 860, 1146
BstKTI GATC 7 cut(s) 117, 742, 762, 919, 950, 972, 1126
BstMAI GTCTC 1 cut(s) 1110
BstMBI GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
BstMCI CGRYCG 1 cut(s) 1126
BstMWI GCNNNNNNNGC 8 cut(s) 35, 184, 728, 761, 782, 848, 857, 878
BstNI CCWGG 6 cut(s) 296, 544, 640, 962, 1004, 1032
BstNSI RCATGY 2 cut(s) 377, 551
BstPAI GACNNNNGTC 2 cut(s) 798, 1162
BstSCI CCNGG 7 cut(s) 294, 542, 638, 960, 1002, 1030, 1209
BstUI CGCG 1 cut(s) 858
BstV1I GCAGC 4 cut(s) 593, 662, 734, 817
BstV2I GAAGAC 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 1271
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 2 cut(s) 87, 187
BtgI CCRYGG 2 cut(s) 182, 942
BtsCI GGATG 4 cut(s) 335, 539, 998, 1197
BtsI GCAGTG 2 cut(s) 591, 783
BtsIMutI CAGTG 4 cut(s) 23, 591, 783, 1216
Cac8I GCNNGC 4 cut(s) 217, 408, 549, 1052
CfoI GCGC 2 cut(s) 860, 1146
Cfr10I RCCGGY 1 cut(s) 1159
Cfr13I GGNCC 1 cut(s) 643
CseI GACGC 3 cut(s) 159, 848, 878
Csp6I GTAC 3 cut(s) 305, 371, 672
CspAI ACCGGT 1 cut(s) 1159
CviAII CATG 7 cut(s) 93, 374, 480, 548, 608, 647, 661
CviQI GTAC 3 cut(s) 305, 371, 672
DdeI CTNAG 8 cut(s) 196, 365, 393, 561, 620, 966, 1236, 1259
DpnI GATC 7 cut(s) 116, 741, 761, 918, 949, 971, 1125
DpnII GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
DrdI GACNNNNNNGTC 1 cut(s) 992
DseDI GACNNNNNNGTC 1 cut(s) 992
Eam1104I CTCTTC 4 cut(s) 195, 336, 1113, 1176
EarI CTCTTC 4 cut(s) 195, 336, 1113, 1176
Eco130I CCWWGG 4 cut(s) 489, 706, 831, 861
Eco147I AGGCCT 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 643
Eco57I CTGAAG 2 cut(s) 786, 1173
EcoRI GAATTC 3 cut(s) 397, 902, 937
EcoRII CCWGG 6 cut(s) 294, 542, 638, 960, 1002, 1030
EcoT14I CCWWGG 4 cut(s) 489, 706, 831, 861
ErhI CCWWGG 4 cut(s) 489, 706, 831, 861
FaeI CATG 7 cut(s) 96, 377, 483, 551, 611, 650, 664
FaqI GGGAC 1 cut(s) 656
FatI CATG 7 cut(s) 92, 373, 479, 547, 607, 646, 660
FauI CCCGC 3 cut(s) 778, 1059, 1085
FblI GTMKAC 2 cut(s) 276, 428
Fnu4HI GCNGC 7 cut(s) 111, 129, 176, 582, 651, 723, 806
FokI GGATG 4 cut(s) 322, 526, 985, 1184
Fsp4HI GCNGC 7 cut(s) 111, 129, 176, 582, 651, 723, 806
FspBI CTAG 2 cut(s) 141, 719
GlaI GCGC 2 cut(s) 859, 1145
GluI GCNGC 7 cut(s) 111, 129, 176, 582, 651, 723, 806
GsaI CCCAGC 2 cut(s) 628, 1075
GsuI CTGGAG 3 cut(s) 250, 897, 1160
HaeII RGCGCY 1 cut(s) 1147
HaeIII GGCC 2 cut(s) 87, 187
HapII CCGG 6 cut(s) 172, 899, 934, 1059, 1160, 1211
HgaI GACGC 3 cut(s) 159, 848, 878
HhaI GCGC 2 cut(s) 860, 1146
Hin1I GRCGYC 3 cut(s) 151, 840, 870
Hin1II CATG 7 cut(s) 96, 377, 483, 551, 611, 650, 664
Hin6I GCGC 2 cut(s) 858, 1144
HinP1I GCGC 2 cut(s) 858, 1144
HincII GTYRAC 4 cut(s) 146, 429, 1242, 1285
HindII GTYRAC 4 cut(s) 146, 429, 1242, 1285
HinfI GANTC 2 cut(s) 122, 681
HpaII CCGG 6 cut(s) 172, 899, 934, 1059, 1160, 1211
HphI GGTGA 7 cut(s) 153, 479, 610, 867, 942, 1003, 1173
Hpy166II GTNNAC 7 cut(s) 146, 277, 429, 601, 1021, 1242, 1285
Hpy188I TCNGA 8 cut(s) 340, 368, 396, 896, 969, 985, 1095, 1157
Hpy188III TCNNGA 2 cut(s) 791, 914
Hpy8I GTNNAC 7 cut(s) 146, 277, 429, 601, 1021, 1242, 1285
Hpy99I CGWCG 4 cut(s) 153, 1019, 1050, 1070
HpyAV CCTTC 5 cut(s) 828, 963, 967, 1052, 1211
HpyCH4III ACNGT 7 cut(s) 18, 309, 512, 1015, 1025, 1220, 1282
HpyCH4V TGCA 9 cut(s) 92, 215, 244, 423, 539, 551, 650, 660, 747
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 184, 728, 761, 782, 848, 857, 878
HpyF3I CTNAG 8 cut(s) 196, 365, 393, 561, 620, 966, 1236, 1259
Hsp92I GRCGYC 3 cut(s) 151, 840, 870
Hsp92II CATG 7 cut(s) 96, 377, 483, 551, 611, 650, 664
HspAI GCGC 2 cut(s) 858, 1144
Kzo9I GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 3 cut(s) 813, 1067, 1090
Lsp1109I GCAGC 4 cut(s) 593, 662, 734, 817
LweI GCATC 4 cut(s) 548, 890, 900, 1206
MaeI CTAG 2 cut(s) 141, 719
MaeIII GTNAC 4 cut(s) 698, 994, 1009, 1179
MalI GATC 7 cut(s) 116, 741, 761, 918, 949, 971, 1125
MboI GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
MboII GAAGA 5 cut(s) 182, 353, 482, 1100, 1163
MluCI AATT 6 cut(s) 356, 397, 631, 713, 902, 937
MlyI GAGTC 1 cut(s) 675
MmeI TCCRAC 1 cut(s) 1248
MnlI CCTC 8 cut(s) 197, 198, 402, 721, 829, 859, 883, 1239
MslI CAYNNNNRTG 2 cut(s) 284, 1269
MspI CCGG 6 cut(s) 172, 899, 934, 1059, 1160, 1211
MspR9I CCNGG 7 cut(s) 296, 544, 640, 962, 1004, 1032, 1211
Mva1269I GAATGC 3 cut(s) 425, 490, 518
MvaI CCWGG 6 cut(s) 296, 544, 640, 962, 1004, 1032
MvnI CGCG 1 cut(s) 858
MwoI GCNNNNNNNGC 8 cut(s) 35, 184, 728, 761, 782, 848, 857, 878
NciI CCSGG 1 cut(s) 1211
NdeII GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
NlaIII CATG 7 cut(s) 96, 377, 483, 551, 611, 650, 664
NlaIV GGNNCC 2 cut(s) 644, 1063
NmuCI GTSAC 4 cut(s) 698, 994, 1009, 1179
NspI RCATGY 2 cut(s) 377, 551
NspV TTCGAA 1 cut(s) 401
OliI CACNNNNGTG 1 cut(s) 1269
PaeI GCATGC 1 cut(s) 551
PceI AGGCCT 1 cut(s) 87
PciI ACATGT 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 336
PcsI WCGNNNNNNNCGW 2 cut(s) 407, 1014
PctI GAATGC 3 cut(s) 425, 490, 518
PfeI GAWTC 1 cut(s) 122
PflFI GACNNNGTC 1 cut(s) 153
PfoI TCCNGGA 1 cut(s) 294
PinAI ACCGGT 1 cut(s) 1159
PkrI GCNGC 7 cut(s) 112, 130, 177, 583, 652, 724, 807
Ple19I CGATCG 1 cut(s) 1126
PleI GAGTC 1 cut(s) 675
PpsI GAGTC 1 cut(s) 675
PscI ACATGT 1 cut(s) 373
PshAI GACNNNNGTC 2 cut(s) 798, 1162
Psp6I CCWGG 6 cut(s) 294, 542, 638, 960, 1002, 1030
PspFI CCCAGC 2 cut(s) 624, 1071
PspGI CCWGG 6 cut(s) 294, 542, 638, 960, 1002, 1030
PspN4I GGNNCC 2 cut(s) 644, 1063
PspPI GGNCC 1 cut(s) 643
PsyI GACNNNGTC 1 cut(s) 153
PvuI CGATCG 1 cut(s) 1126
RsaI GTAC 3 cut(s) 306, 372, 673
RsaNI GTAC 3 cut(s) 305, 371, 672
RseI CAYNNNNRTG 2 cut(s) 284, 1269
SalI GTCGAC 1 cut(s) 427
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 7 cut(s) 111, 129, 176, 582, 651, 723, 806
Sau3AI GATC 7 cut(s) 114, 739, 759, 916, 947, 969, 1123
Sau96I GGNCC 1 cut(s) 643
SchI GAGTC 1 cut(s) 675
ScrFI CCNGG 7 cut(s) 296, 544, 640, 962, 1004, 1032, 1211
SfaNI GCATC 4 cut(s) 548, 890, 900, 1206
SfuI TTCGAA 1 cut(s) 401
SinI GGWCC 1 cut(s) 643
SmiMI CAYNNNNRTG 2 cut(s) 284, 1269
SpeI ACTAGT 1 cut(s) 140
SphI GCATGC 1 cut(s) 551
Sse9I AATT 6 cut(s) 356, 397, 631, 713, 902, 937
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 6 cut(s) 110, 128, 175, 785, 1052, 1078
SspMI CTAG 2 cut(s) 141, 719
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 7 cut(s) 294, 542, 638, 960, 1002, 1030, 1209
StyI CCWWGG 4 cut(s) 489, 706, 831, 861
TaaI ACNGT 7 cut(s) 18, 309, 512, 1015, 1025, 1220, 1282
TaqI TCGA 7 cut(s) 42, 209, 323, 401, 428, 514, 1122
TaqII GACCGA 1 cut(s) 216
TasI AATT 6 cut(s) 356, 397, 631, 713, 902, 937
TatI WGTACW 3 cut(s) 304, 370, 671
TauI GCSGC 3 cut(s) 113, 131, 178
TfiI GAWTC 1 cut(s) 122
TscAI CASTG 4 cut(s) 23, 591, 783, 1223
TseFI GTSAC 4 cut(s) 698, 994, 1009, 1179
TseI GCWGC 4 cut(s) 581, 650, 722, 805
Tsp45I GTSAC 4 cut(s) 698, 994, 1009, 1179
TspDTI ATGAA 2 cut(s) 677, 810
TspGWI ACGGA 5 cut(s) 20, 46, 1054, 1103, 1140
TspRI CASTG 4 cut(s) 23, 591, 783, 1223
Tth111I GACNNNGTC 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 643
XapI RAATTY 5 cut(s) 356, 397, 713, 902, 937
XceI RCATGY 2 cut(s) 377, 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 1028
XmiI GTMKAC 2 cut(s) 276, 428
XspI CTAG 2 cut(s) 141, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.