Rmu_sc0000585.1_g000009

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000585.1
OG0021635
Physical Location & Seq
Forward (+)
40435 .. 42588
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
atgatggaaaaggtcacattagagtgtgaaatgataaaaatgtcacctagtttcccacttgataaaatgaaggactctaatgtctccggcgacgatgccttcactcgcctctactctgccgtcgagttcgacattgaagctgctcttcaaaaagcggagcttgcttcaaaagagaaaactagggcatttgcagttgctctgaatgccgtgattcgccgtaccaaggctcgattgcttaaagaggttcccaagttgcagagattggctgtaaagaaggttaaagggctttcatctgaagagtttgctgctcgtaatgagttgttgtaccagttcatacctaatgcaagagttcccattctgatggtcaaaagtaatcttggaaacatctcctgtgatatttcagtcaataacctgaagggccaaatgaaatcaaaactctttttatggatgaatgatatagatccacgatttcgtgatatggttttactggtgaaggaatgggcaaaagcacacaatatcaataattcaaaatctgggactttcaattcatactctctgagtttgctcgtgatcttccatttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

194

Amino Acids

22.05

Weight (kDa)

9.58

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 109 - 147 2e-06 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021635)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000585.1_g000009
rosa_roxburghii Rroxscaffold_4G00295580
rosa_samantha Rh6CG100600 Rh6DG092800 Rh7CG207300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AciI CCGC 1 cut(s) 155
AclWI GGATC 1 cut(s) 455
AcuI CTGAAG 2 cut(s) 315, 434
AfaI GTAC 2 cut(s) 220, 326
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 5 cut(s) 137, 149, 168, 528, 544
AluBI AGCT 2 cut(s) 140, 160
AluI AGCT 2 cut(s) 140, 160
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 1 cut(s) 455
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 2 cut(s) 140, 305
AspS9I GGNCC 1 cut(s) 418
AsuHPI GGTGA 2 cut(s) 36, 502
BauI CACGAG 1 cut(s) 566
BbvI GCAGC 2 cut(s) 127, 292
BccI CCATC 1 cut(s) 355
BceAI ACGGC 3 cut(s) 104, 191, 201
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 2 cut(s) 48, 180
BisI GCNGC 2 cut(s) 141, 306
BlsI GCNGC 2 cut(s) 142, 307
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 1 cut(s) 246
BmsI GCATC 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 2 cut(s) 328, 492
Bse8I GATNNNNATC 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 1 cut(s) 453
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 548
BseNI ACTGG 2 cut(s) 328, 492
BseXI GCAGC 2 cut(s) 127, 292
BshFI GGCC 1 cut(s) 420
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 550
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 420
Bsp143I GATC 2 cut(s) 460, 570
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 1 cut(s) 549
BspLI GGNNCC 1 cut(s) 246
BspPI GGATC 1 cut(s) 455
BspQI GCTCTTC 1 cut(s) 150
BsrI ACTGG 2 cut(s) 328, 492
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 460, 570
BssSI CACGAG 1 cut(s) 566
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 1 cut(s) 566
Bst6I CTCTTC 2 cut(s) 150, 291
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 453
BstKTI GATC 2 cut(s) 463, 573
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 2 cut(s) 460, 570
BstMWI GCNNNNNNNGC 2 cut(s) 161, 203
BstV1I GCAGC 2 cut(s) 127, 292
BstX2I RGATCY 1 cut(s) 460
BstXI CCANNNNNNTGG 1 cut(s) 361
BstYI RGATCY 1 cut(s) 460
BsuRI GGCC 1 cut(s) 420
BtsCI GGATG 1 cut(s) 453
Cac8I GCNNGC 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 418
Csp6I GTAC 2 cut(s) 219, 325
CviJI RGCY 6 cut(s) 140, 160, 227, 266, 286, 420
CviKI_1 RGCY 6 cut(s) 140, 160, 227, 266, 286, 420
CviQI GTAC 2 cut(s) 219, 325
DdeI CTNAG 1 cut(s) 557
DpnI GATC 2 cut(s) 462, 572
DpnII GATC 2 cut(s) 460, 570
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
Eam1104I CTCTTC 2 cut(s) 150, 291
EarI CTCTTC 2 cut(s) 150, 291
Eco130I CCWWGG 1 cut(s) 222
Eco57I CTGAAG 2 cut(s) 315, 434
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaiI YATR 5 cut(s) 335, 445, 458, 479, 550
FalI AAGNNNNNCTT 4 cut(s) 129, 161, 144, 176
FaqI GGGAC 1 cut(s) 550
Fnu4HI GCNGC 2 cut(s) 141, 306
FokI GGATG 1 cut(s) 460
Fsp4HI GCNGC 2 cut(s) 141, 306
FspBI CTAG 2 cut(s) 48, 180
GluI GCNGC 2 cut(s) 141, 306
HaeIII GGCC 1 cut(s) 420
HapII CCGG 1 cut(s) 87
HinfI GANTC 2 cut(s) 74, 211
HpaII CCGG 1 cut(s) 87
HphI GGTGA 2 cut(s) 36, 502
Hpy188I TCNGA 4 cut(s) 201, 295, 360, 558
Hpy188III TCNNGA 2 cut(s) 473, 568
Hpy99I CGWCG 2 cut(s) 95, 125
HpyAV CCTTC 5 cut(s) 64, 109, 268, 409, 487
HpyCH4V TGCA 3 cut(s) 191, 256, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 203
HpyF3I CTNAG 1 cut(s) 557
Kzo9I GATC 2 cut(s) 460, 570
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 6 cut(s) 100, 341, 403, 425, 473, 519
Lsp1109I GCAGC 2 cut(s) 127, 292
LweI GCATC 1 cut(s) 85
MaeI CTAG 2 cut(s) 48, 180
MaeIII GTNAC 2 cut(s) 13, 42
MalI GATC 2 cut(s) 462, 572
MboI GATC 2 cut(s) 460, 570
MboII GAAGA 3 cut(s) 137, 308, 565
MflI RGATCY 1 cut(s) 460
MluCI AATT 2 cut(s) 523, 544
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 2 cut(s) 119, 235
MseI TTAA 2 cut(s) 237, 279
MslI CAYNNNNRTG 2 cut(s) 22, 359
MspI CCGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 161, 203
NdeII GATC 2 cut(s) 460, 570
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 2 cut(s) 13, 42
PciSI GCTCTTC 1 cut(s) 150
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 211
PkrI GCNGC 2 cut(s) 142, 307
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 1 cut(s) 418
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 2 cut(s) 220, 326
RsaNI GTAC 2 cut(s) 219, 325
RseI CAYNNNNRTG 2 cut(s) 22, 359
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 2 cut(s) 237, 279
SatI GCNGC 2 cut(s) 141, 306
Sau3AI GATC 2 cut(s) 460, 570
Sau96I GGNCC 1 cut(s) 418
SchI GAGTC 1 cut(s) 68
SetI ASST 8 cut(s) 15, 49, 142, 162, 246, 279, 340, 414
SfaNI GCATC 1 cut(s) 85
SmiMI CAYNNNNRTG 2 cut(s) 22, 359
Sse9I AATT 2 cut(s) 523, 544
SsiI CCGC 1 cut(s) 155
SspMI CTAG 2 cut(s) 48, 180
StyI CCWWGG 1 cut(s) 222
TaqI TCGA 3 cut(s) 123, 129, 229
TasI AATT 2 cut(s) 523, 544
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 2 cut(s) 237, 279
Tru9I TTAA 2 cut(s) 237, 279
TseFI GTSAC 2 cut(s) 13, 42
TseI GCWGC 2 cut(s) 140, 305
Tsp45I GTSAC 2 cut(s) 13, 42
TspDTI ATGAA 6 cut(s) 83, 279, 322, 440, 464, 537
XspI CTAG 2 cut(s) 48, 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.