Rroxscaffold_4G00295580

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0021635
Physical Location & Seq
Reverse (-)
15569240 .. 15571549
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295580.1

Sequence Viewer

Length: 513 bp
ATGGAACAGTACCAGTTCATACCTAATGCAAGAGTTCCCATTTTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCCGAAAGGCCAAATGAAATCAAAACTCTTTTTATGGATGAATGATATAGATCCACGCTTTCGTGATATGGTTTTACGTTTGTGCCCAAGCAAAGTGGAACTCCTAAGAAAAATGAGATCATATTTACGGCTCCAATCGGAGAGGAGATCTCCAACGAGAAAACAGTGGAACAATACCGAAGGCACACACGGTGGCCCTTCGGTGTCGGAGTTTGATTGGGGCACGGTGAGACCCCAAGGAGATTGGCTAGGATTAGTGAGAAGGTCAAGGGCAACTCCATCCCCAGAGAGTGATCCCCCAAAGAAGCGAAGCAGAAAAACATCGGCTTCGAAGAAGGATAGCAAAGAAAAGGAAGCTGCTCCTCAAGAAACCGAGGGGACAAAAGTTAGTCATGTGCAAGATGCGATAAAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

170

Amino Acids

19.45

Weight (kDa)

10.09

Isoelectric Point (pI)

74.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 1 - 42 2.8e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021635)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000585.1_g000009
rosa_roxburghii Rroxscaffold_4G00295580
rosa_samantha Rh6CG100600 Rh6DG092800 Rh7CG207300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 140, 383
AdeI CACNNNGTG 1 cut(s) 287
AfaI GTAC 1 cut(s) 11
AluBI AGCT 1 cut(s) 452
AluI AGCT 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 2 cut(s) 140, 383
AoxI GGCC 2 cut(s) 103, 289
ApeKI GCWGC 1 cut(s) 452
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 425
BaeGI GKGCMC 2 cut(s) 182, 320
BbvI GCAGC 1 cut(s) 439
BccI CCATC 2 cut(s) 40, 382
BceAI ACGGC 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 344
BglII AGATCT 1 cut(s) 242
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 227
BmsI GCATC 1 cut(s) 487
BplI GAGNNNNNCTC 2 cut(s) 229, 261
Bpu14I TTCGAA 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 444
BsaBI GATNNNNATC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 331, 468
Bse1I ACTGG 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 331, 468
BseGI GGATG 2 cut(s) 138, 374
BseJI GATNNNNATC 1 cut(s) 144
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 2 cut(s) 253, 447
BseSI GKGCMC 2 cut(s) 182, 320
BseXI GCAGC 1 cut(s) 439
BshFI GGCC 2 cut(s) 105, 291
BslFI GGGAC 1 cut(s) 487
BsmAI GTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 2 cut(s) 105, 291
Bso31I GGTCTC 1 cut(s) 319
Bsp119I TTCGAA 1 cut(s) 425
Bsp1286I GDGCHC 2 cut(s) 182, 320
Bsp143I GATC 4 cut(s) 145, 212, 242, 388
BspANI GGCC 2 cut(s) 105, 291
BspLI GGNNCC 1 cut(s) 227
BspPI GGATC 2 cut(s) 140, 383
BspT104I TTCGAA 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 319
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 331, 468
BssMI GATC 4 cut(s) 145, 212, 242, 388
BssT1I CCWWGG 1 cut(s) 331
Bst4CI ACNGT 4 cut(s) 9, 261, 287, 322
BstBI TTCGAA 1 cut(s) 425
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 2 cut(s) 138, 374
BstKTI GATC 4 cut(s) 148, 215, 245, 391
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 4 cut(s) 145, 212, 242, 388
BstSLI GKGCMC 2 cut(s) 182, 320
BstV1I GCAGC 1 cut(s) 439
BstX2I RGATCY 2 cut(s) 145, 242
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 2 cut(s) 145, 242
BsuRI GGCC 2 cut(s) 105, 291
BtsCI GGATG 2 cut(s) 138, 374
BtsIMutI CAGTG 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 1 cut(s) 488
CviJI RGCY 6 cut(s) 105, 226, 291, 343, 422, 452
CviKI_1 RGCY 6 cut(s) 105, 226, 291, 343, 422, 452
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 1 cut(s) 200
DpnI GATC 4 cut(s) 147, 214, 244, 390
DpnII GATC 4 cut(s) 145, 212, 242, 388
DraIII CACNNNGTG 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 331
Eco31I GGTCTC 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 1 cut(s) 491
FaiI YATR 6 cut(s) 20, 130, 143, 164, 217, 489
FaqI GGGAC 1 cut(s) 487
FatI CATG 1 cut(s) 487
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 2 cut(s) 145, 361
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 1 cut(s) 344
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 2 cut(s) 105, 291
Hin1II CATG 1 cut(s) 491
HphI GGTGA 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 235, 304
Hpy188III TCNNGA 3 cut(s) 158, 461, 510
HpyAV CCTTC 4 cut(s) 269, 303, 351, 424
HpyCH4III ACNGT 4 cut(s) 9, 261, 287, 322
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 2 cut(s) 29, 493
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 1 cut(s) 491
Kzo9I GATC 4 cut(s) 145, 212, 242, 388
LmnI GCTCC 2 cut(s) 231, 460
LpnPI CCDG 3 cut(s) 26, 88, 393
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 1 cut(s) 487
MaeI CTAG 1 cut(s) 344
MaeII ACGT 1 cut(s) 172
MalI GATC 4 cut(s) 147, 214, 244, 390
MboI GATC 4 cut(s) 145, 212, 242, 388
MboII GAAGA 1 cut(s) 439
MflI RGATCY 2 cut(s) 145, 242
MhlI GDGCHC 2 cut(s) 182, 320
MmeI TCCRAC 2 cut(s) 272, 282
MnlI CCTC 3 cut(s) 231, 463, 468
MslI CAYNNNNRTG 1 cut(s) 44
NdeII GATC 4 cut(s) 145, 212, 242, 388
NlaIII CATG 1 cut(s) 491
NlaIV GGNNCC 1 cut(s) 227
NspV TTCGAA 1 cut(s) 425
PkrI GCNGC 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 1 cut(s) 290
PsuI RGATCY 2 cut(s) 145, 242
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 44
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 4 cut(s) 145, 212, 242, 388
Sau96I GGNCC 1 cut(s) 290
SduI GDGCHC 2 cut(s) 182, 320
SetI ASST 4 cut(s) 25, 175, 362, 454
SfaNI GCATC 1 cut(s) 487
SfuI TTCGAA 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
SspMI CTAG 1 cut(s) 344
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 4 cut(s) 9, 261, 287, 322
TaiI ACGT 1 cut(s) 175
TaqI TCGA 1 cut(s) 425
TscAI CASTG 1 cut(s) 266
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 3 cut(s) 7, 125, 149
TspRI CASTG 1 cut(s) 266
XspI CTAG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.