Rh6CG100600

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0021635
Physical Location & Seq
Forward (+)
11159952 .. 11164450
4499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG100600.1

Sequence Viewer

Length: 774 bp
ATGGTGAAGGGCGGCGAGTCGGAGAAGTCGAGGAAGGTCTTAAGGAGTCGCGGGGTCAAGGAGGTTGGGATGAAAGCGGAACTTGCTTCAAAAGAGAAAAGTAGGGCATTTGCAGTTGCTCTGGATGCCGTGATTTGCCGGACCAAGGCTCAATTGCTTAAAGAGGTTCCCAAGTTGCAGAGATTGGCTGTAAAGAAGATTCATAGAAGAAAGGTACGGGCATGGCAAATGATATGTGCAACTATGGAGCCATTTGGATCATTTGTCTCAGAACTCTTTACTCGCTGGGGAGACTTGGATGTTTCAATTGAGTTCTCAAAAGGCGCATATATTTCTTCTTATGGAAGAAAGCACACACAAGATGTACTAAAAGATTTAATGAAGGCTATGAGAAAGAGAGGTGGATGGAACAGGTACCAGTTCATACCTAATGCAAGAGTTCCCATTCTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCTGAAGGGCCAAATGAAATCAAAACTCTTTTTATGGATGAATGATATAGATCCACGCTTTCGTGATATGGTTTTACTGGTGAAGGAATGGGCAAAAGCACACAATATCAATAATTCAAAATCTGGGACTTTCAATTCATACTCTCTGAGTTTGCTCGTGATCTTCCATTTTCAGACATGTACACCTGCAATTCTGTCAATTCTGTCTCCTCTTCAAGACATATATACAGAAACTCTCACGGATGATCTTAAAGATATGGTACTGGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

257

Amino Acids

29.36

Weight (kDa)

9.88

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 58 - 177 2.2e-19 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021635)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000585.1_g000009
rosa_roxburghii Rroxscaffold_4G00295580
rosa_samantha Rh6CG100600 Rh6DG092800 Rh7CG207300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 694
Acc36I ACCTGC 1 cut(s) 694
Acc65I GGTACC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 414
AccII CGCG 1 cut(s) 51
AciI CCGC 3 cut(s) 12, 51, 77
AclWI GGATC 2 cut(s) 265, 545
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 5 cut(s) 216, 366, 416, 682, 762
AfiI CCNNNNNNNGG 1 cut(s) 145
AflII CTTAAG 1 cut(s) 40
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 5 cut(s) 90, 306, 618, 634, 716
Alw26I GTCTC 3 cut(s) 271, 285, 711
AlwI GGATC 2 cut(s) 265, 545
AoxI GGCC 1 cut(s) 508
Asp718I GGTACC 1 cut(s) 414
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 2 cut(s) 141, 508
AsuHPI GGTGA 2 cut(s) 16, 592
AvaII GGWCC 1 cut(s) 141
BanI GGYRCC 1 cut(s) 414
BauI CACGAG 1 cut(s) 656
BccI CCATC 2 cut(s) 399, 445
BceAI ACGGC 1 cut(s) 113
BcoDI GTCTC 3 cut(s) 271, 285, 711
BfaI CTAG 1 cut(s) 772
BfrI CTTAAG 1 cut(s) 40
BfuAI ACCTGC 1 cut(s) 694
BisI GCNGC 1 cut(s) 13
BlsI GCNGC 1 cut(s) 14
Bme18I GGWCC 1 cut(s) 141
BmgT120I GGNCC 2 cut(s) 141, 508
BmiI GGNNCC 3 cut(s) 168, 249, 416
BmsI GCATC 1 cut(s) 115
BsaBI GATNNNNATC 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 144
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 3 cut(s) 418, 582, 768
Bse8I GATNNNNATC 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 6 cut(s) 75, 130, 304, 410, 543, 748
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 282, 638
BseNI ACTGG 3 cut(s) 418, 582, 768
BseRI GAGGAG 1 cut(s) 699
BseYI CCCAGC 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 510
BshNI GGYRCC 1 cut(s) 414
BsiSI CCGG 1 cut(s) 139
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 3 cut(s) 271, 285, 711
BsmFI GGGAC 1 cut(s) 640
BsnI GGCC 1 cut(s) 510
Bsp1407I TGTACA 1 cut(s) 680
Bsp143I GATC 4 cut(s) 257, 550, 660, 745
BspACI CCGC 3 cut(s) 12, 51, 77
BspANI GGCC 1 cut(s) 510
BspCNI CTCAG 2 cut(s) 281, 639
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 168, 249, 416
BspMI ACCTGC 1 cut(s) 694
BspPI GGATC 2 cut(s) 265, 545
BspT107I GGYRCC 1 cut(s) 414
BspTI CTTAAG 1 cut(s) 40
BsrGI TGTACA 1 cut(s) 680
BsrI ACTGG 3 cut(s) 418, 582, 768
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 4 cut(s) 257, 550, 660, 745
BssSI CACGAG 1 cut(s) 656
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 656
Bst6I CTCTTC 1 cut(s) 717
BstAFI CTTAAG 1 cut(s) 40
BstAUI TGTACA 1 cut(s) 680
BstDEI CTNAG 2 cut(s) 268, 647
BstF5I GGATG 6 cut(s) 75, 130, 304, 410, 543, 748
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 4 cut(s) 260, 553, 663, 748
BstMAI GTCTC 3 cut(s) 271, 285, 711
BstMBI GATC 4 cut(s) 257, 550, 660, 745
BstMWI GCNNNNNNNGC 2 cut(s) 83, 125
BstNSI RCATGY 1 cut(s) 681
BstUI CGCG 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 1 cut(s) 451
BstYI RGATCY 1 cut(s) 550
BsuRI GGCC 1 cut(s) 510
BtsCI GGATG 6 cut(s) 75, 130, 304, 410, 543, 748
BveI ACCTGC 1 cut(s) 694
CfoI GCGC 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 141, 508
Csp6I GTAC 5 cut(s) 215, 365, 415, 681, 761
CviAII CATG 2 cut(s) 222, 678
CviJI RGCY 5 cut(s) 149, 188, 250, 386, 510
CviKI_1 RGCY 5 cut(s) 149, 188, 250, 386, 510
CviQI GTAC 5 cut(s) 215, 365, 415, 681, 761
DdeI CTNAG 2 cut(s) 268, 647
DpnI GATC 4 cut(s) 259, 552, 662, 747
DpnII GATC 4 cut(s) 257, 550, 660, 745
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
Eco130I CCWWGG 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 524
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 2 cut(s) 225, 681
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 1 cut(s) 640
FatI CATG 2 cut(s) 221, 677
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 13
FokI GGATG 6 cut(s) 82, 137, 311, 417, 550, 755
Fsp4HI GCNGC 1 cut(s) 13
FspBI CTAG 1 cut(s) 772
GlaI GCGC 1 cut(s) 325
GluI GCNGC 1 cut(s) 13
GsaI CCCAGC 1 cut(s) 289
HaeIII GGCC 1 cut(s) 510
HapII CCGG 1 cut(s) 139
HhaI GCGC 1 cut(s) 326
Hin1II CATG 2 cut(s) 225, 681
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HinfI GANTC 3 cut(s) 17, 46, 199
HpaII CCGG 1 cut(s) 139
HphI GGTGA 2 cut(s) 16, 592
Hpy166II GTNNAC 1 cut(s) 683
Hpy188I TCNGA 5 cut(s) 22, 271, 450, 648, 675
Hpy188III TCNNGA 4 cut(s) 122, 563, 658, 716
Hpy8I GTNNAC 1 cut(s) 683
HpyAV CCTTC 4 cut(s) 28, 376, 499, 577
HpyCH4V TGCA 5 cut(s) 113, 178, 239, 434, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 125
HpyF3I CTNAG 2 cut(s) 268, 647
Hsp92II CATG 2 cut(s) 225, 681
HspAI GCGC 1 cut(s) 324
KpnI GGTACC 1 cut(s) 418
Kzo9I GATC 4 cut(s) 257, 550, 660, 745
LmnI GCTCC 1 cut(s) 247
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 772
MalI GATC 4 cut(s) 259, 552, 662, 747
MboI GATC 4 cut(s) 257, 550, 660, 745
MboII GAAGA 6 cut(s) 208, 219, 327, 357, 655, 704
MfeI CAATTG 2 cut(s) 152, 306
MflI RGATCY 1 cut(s) 550
MluCI AATT 6 cut(s) 152, 306, 613, 634, 690, 699
MlyI GAGTC 2 cut(s) 26, 55
MnlI CCTC 5 cut(s) 24, 55, 157, 392, 720
MseI TTAA 4 cut(s) 41, 159, 377, 750
MslI CAYNNNNRTG 1 cut(s) 449
MspCI CTTAAG 1 cut(s) 40
MspI CCGG 1 cut(s) 139
MunI CAATTG 2 cut(s) 152, 306
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 83, 125
NdeII GATC 4 cut(s) 257, 550, 660, 745
NlaIII CATG 2 cut(s) 225, 681
NlaIV GGNNCC 3 cut(s) 168, 249, 416
NspI RCATGY 1 cut(s) 681
PaqCI CACCTGC 1 cut(s) 694
PciI ACATGT 1 cut(s) 677
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 14
PleI GAGTC 2 cut(s) 25, 54
PpsI GAGTC 2 cut(s) 25, 54
PscI ACATGT 1 cut(s) 677
PspFI CCCAGC 1 cut(s) 285
PspN4I GGNNCC 3 cut(s) 168, 249, 416
PspPI GGNCC 2 cut(s) 141, 508
PsuI RGATCY 1 cut(s) 550
RsaI GTAC 5 cut(s) 216, 366, 416, 682, 762
RsaNI GTAC 5 cut(s) 215, 365, 415, 681, 761
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 4 cut(s) 41, 159, 377, 750
SatI GCNGC 1 cut(s) 13
Sau3AI GATC 4 cut(s) 257, 550, 660, 745
Sau96I GGNCC 2 cut(s) 141, 508
SchI GAGTC 2 cut(s) 26, 55
SetI ASST 9 cut(s) 39, 66, 168, 216, 403, 416, 430, 504, 688
SfaNI GCATC 1 cut(s) 115
SinI GGWCC 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse9I AATT 6 cut(s) 152, 306, 613, 634, 690, 699
SsiI CCGC 3 cut(s) 12, 51, 77
SspMI CTAG 1 cut(s) 772
StyI CCWWGG 1 cut(s) 144
TaqI TCGA 1 cut(s) 29
TasI AATT 6 cut(s) 152, 306, 613, 634, 690, 699
TatI WGTACW 2 cut(s) 364, 680
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 4 cut(s) 41, 159, 377, 750
Tru9I TTAA 4 cut(s) 41, 159, 377, 750
TspDTI ATGAA 7 cut(s) 86, 191, 395, 412, 530, 554, 627
TspGWI ACGGA 1 cut(s) 755
Vha464I CTTAAG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 141
XceI RCATGY 1 cut(s) 681
XspI CTAG 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.