Rmu_sc0000616.1_g000008

Tubulin-specific chaperone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000616.1
OG0004433
Physical Location & Seq
Forward (+)
49865 .. 56360
6496 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1509 bp
atgcaaccctcatcggaatcaaacccagaattcaaactcggccagcgagtccactcggctggtgacccccgccgaatcggaacagtgaggtacttgggacccgttgaaggctactcgggcacatgggtcggagtcgattgggacaacagagacggcaagcatgatgggtccgtcaatggcgtcaactacttccacgcaaggtctgaaacatctggctctttcgttcgatcccacaatctcagctctggaatctcattgctgcaagctcttgagctcagatatcgaggccaaaccactcaagaagaagaggatgaaatgtatgtactttctgcgagcaacaagcgtgtgagtgttcaacttgttggtaaagacaagattcaagacaaactgagttgtttcgaagagttgactagtgcatcaacatcttacttgggtgttagctctcctggagacccatctgaaattggtgctgttgtgccaaatttgaaggagcttgacttgactggaaatctaatttcagagtggaaggatgttggcatgatctgtgaacagctgcctgatcttgttgccctcaatttatcttacaatttaatgtcacatgatattgttgggttgccacatttgaaaagcatccgtattttacctgcatcttcttcaacagttcagggatttgattatttgcggctactgaatttggacgacaattgtataactgattggaatgaaatcttgaaactttcacagctaccaagcttggagcagttgcatttggacaataacaatttgaaccatgtcttttatcctgatgttggcatgatgcttgagctgctcaatggtcatgagtcgcatgaaaaagggcataagccatttcaaaatttgcgttgccttcttcttggaggcaataccattgaggaccctacttctattgatgcactgaactcattcccacaattggtggatgtcagactttctggtaatccagtggctgatccaggacagggtggtatcccaagatttgttttggttgcccgcttaggaaaagttgaaatgttgaatgggagtgaggttacttctcgtgaaagaaaagagtctgaaattcggtatgtccggttagttatgtcaaagcaaggaaatattcaagaaatcaagctgcttcatccaaggtttgccgagctcaaggcttttcatggaattgaggacgaaaagccattggtgggagcagcaggccctcaaaagatggctacagggcttctatctgttactctgaaatgcgtgggagcatcaataggtgagaagccctctttggtgaagaaacttccaggcaccacaacggttggtaagctaaaaattctttgtgtaagctttttcaagctgaagtctatgaagttgaaattgtttcttcaagaagagggttctcctttgccaatgttgctagatgatgaaatgcagagtttgacagaccttgggattggcaatgaatcaaccattcttgtggatgaagagagttaa

Protein Analysis

502

Amino Acids

55.23

Weight (kDa)

5.41

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP_GLY PF01302 13 - 79 1.5e-21 CAP-Gly domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 652
AccB1I GGYRCC 1 cut(s) 1322
AciI CCGC 3 cut(s) 70, 682, 1030
AclWI GGATC 2 cut(s) 222, 983
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 6 cut(s) 29, 481, 691, 874, 1095, 1347
AcuI CTGAAG 1 cut(s) 1394
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 2 cut(s) 92, 324
AfiI CCNNNNNNNGG 5 cut(s) 107, 809, 952, 998, 1214
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 3 cut(s) 445, 991, 1318
AjuI GAANNNNNNNTTGG 2 cut(s) 1286, 1318
Alw21I GWGCWC 2 cut(s) 276, 1176
Alw26I GTCTC 2 cut(s) 144, 444
AlwI GGATC 2 cut(s) 222, 983
AlwNI CAGNNNCTG 1 cut(s) 986
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 3 cut(s) 40, 286, 1225
ApeKI GCWGC 5 cut(s) 259, 553, 826, 1150, 1220
ApoI RAATTY 6 cut(s) 29, 481, 691, 874, 1095, 1347
Asp700I GAANNNNTTC 1 cut(s) 941
AspS9I GGNCC 4 cut(s) 98, 168, 913, 1226
AsuHPI GGTGA 3 cut(s) 74, 1301, 1318
AsuII TTCGAA 1 cut(s) 399
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 3 cut(s) 98, 168, 913
BaeGI GKGCMC 1 cut(s) 122
BanI GGYRCC 1 cut(s) 1322
BanII GRGCYC 2 cut(s) 276, 1176
BauI CACGAG 1 cut(s) 1074
Bbv12I GWGCWC 2 cut(s) 276, 1176
BbvI GCAGC 5 cut(s) 246, 540, 813, 1137, 1232
BccI CCATC 3 cut(s) 158, 463, 1231
BceAI ACGGC 1 cut(s) 169
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 3 cut(s) 447, 993, 1320
BciVI GTATCC 1 cut(s) 1016
BcoDI GTCTC 2 cut(s) 144, 444
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 2 cut(s) 411, 1433
BfmI CTRYAG 1 cut(s) 1242
BfuAI ACCTGC 1 cut(s) 652
BfuI GTATCC 1 cut(s) 1016
BisI GCNGC 6 cut(s) 260, 554, 683, 827, 1151, 1221
BlsI GCNGC 6 cut(s) 261, 555, 684, 828, 1152, 1222
Bme1390I CCNGG 3 cut(s) 447, 993, 1320
Bme18I GGWCC 3 cut(s) 98, 168, 913
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 98, 168, 913, 1226
BmiI GGNNCC 5 cut(s) 99, 100, 169, 915, 1324
BmrFI CCNGG 3 cut(s) 447, 993, 1320
BmsI GCATC 6 cut(s) 425, 639, 656, 807, 919, 1289
BplI GAGNNNNNCTC 2 cut(s) 1283, 1315
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 1 cut(s) 1033
Bpu14I TTCGAA 1 cut(s) 399
BpuEI CTTGAG 4 cut(s) 282, 290, 842, 1160
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 2 cut(s) 1160, 1462
BsaWI WCCGGW 1 cut(s) 1107
Bsc4I CCNNNNNNNGG 5 cut(s) 107, 809, 952, 998, 1214
Bse1I ACTGG 2 cut(s) 508, 980
Bse3DI GCAATG 2 cut(s) 254, 1480
BseBI CCWGG 3 cut(s) 447, 993, 1320
BseDI CCNNGG 2 cut(s) 1160, 1462
BseGI GGATG 6 cut(s) 316, 535, 630, 964, 1156, 1501
BseLI CCNNNNNNNGG 5 cut(s) 107, 809, 952, 998, 1214
BseMI GCAATG 2 cut(s) 254, 1480
BseMII CTCAG 3 cut(s) 253, 289, 380
BseNI ACTGG 2 cut(s) 508, 980
BseSI GKGCMC 1 cut(s) 122
BseXI GCAGC 5 cut(s) 246, 540, 813, 1137, 1232
BshFI GGCC 3 cut(s) 42, 288, 1227
BshNI GGYRCC 1 cut(s) 1322
BsiHKAI GWGCWC 2 cut(s) 276, 1176
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 1 cut(s) 1108
BslFI GGGAC 2 cut(s) 111, 155
BslI CCNNNNNNNGG 5 cut(s) 107, 809, 952, 998, 1214
BsmAI GTCTC 2 cut(s) 144, 444
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 2 cut(s) 111, 155
BsnI GGCC 3 cut(s) 42, 288, 1227
Bso31I GGTCTC 1 cut(s) 444
BsoBI CYCGRG 1 cut(s) 115
Bsp119I TTCGAA 1 cut(s) 399
Bsp1286I GDGCHC 3 cut(s) 122, 276, 1176
Bsp143I GATC 4 cut(s) 227, 540, 559, 988
BspACI CCGC 3 cut(s) 70, 682, 1030
BspANI GGCC 3 cut(s) 42, 288, 1227
BspCNI CTCAG 3 cut(s) 252, 288, 381
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 5 cut(s) 99, 100, 169, 915, 1324
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 2 cut(s) 222, 983
BspT104I TTCGAA 1 cut(s) 399
BspT107I GGYRCC 1 cut(s) 1322
BspTNI GGTCTC 1 cut(s) 444
BsrDI GCAATG 2 cut(s) 254, 1480
BsrI ACTGG 2 cut(s) 508, 980
BssECI CCNNGG 2 cut(s) 1160, 1462
BssMI GATC 4 cut(s) 227, 540, 559, 988
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 1074
BssT1I CCWWGG 2 cut(s) 1160, 1462
Bst2BI CACGAG 1 cut(s) 1074
Bst2UI CCWGG 3 cut(s) 447, 993, 1320
Bst4CI ACNGT 3 cut(s) 85, 661, 1333
Bst6I CTCTTC 4 cut(s) 300, 396, 1401, 1494
BstACI GRCGYC 1 cut(s) 180
BstBI TTCGAA 1 cut(s) 399
BstC8I GCNNGC 6 cut(s) 44, 158, 264, 334, 1030, 1225
BstDEI CTNAG 4 cut(s) 239, 275, 389, 1033
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 6 cut(s) 316, 535, 630, 964, 1156, 1501
BstKTI GATC 4 cut(s) 230, 543, 562, 991
BstMAI GTCTC 2 cut(s) 144, 444
BstMBI GATC 4 cut(s) 227, 540, 559, 988
BstMWI GCNNNNNNNGC 3 cut(s) 117, 826, 1429
BstNI CCWGG 3 cut(s) 447, 993, 1320
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 3 cut(s) 445, 991, 1318
BstSFI CTRYAG 1 cut(s) 1242
BstSLI GKGCMC 1 cut(s) 122
BstV1I GCAGC 5 cut(s) 246, 540, 813, 1137, 1232
BstXI CCANNNNNNTGG 2 cut(s) 59, 1492
BsuI GTATCC 1 cut(s) 1016
BsuRI GGCC 3 cut(s) 42, 288, 1227
BtsCI GGATG 6 cut(s) 316, 535, 630, 964, 1156, 1501
BtsIMutI CAGTG 3 cut(s) 90, 932, 987
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 6 cut(s) 44, 158, 264, 334, 1030, 1225
CaiI CAGNNNCTG 1 cut(s) 986
CciI TCATGA 1 cut(s) 838
Cfr13I GGNCC 4 cut(s) 98, 168, 913, 1226
CseI GACGC 1 cut(s) 169
Csp6I GTAC 2 cut(s) 91, 323
CspCI CAANNNNNGTGG 2 cut(s) 1315, 1350
CviAII CATG 9 cut(s) 123, 161, 538, 599, 791, 814, 839, 848, 1187
CviQI GTAC 2 cut(s) 91, 323
DdeI CTNAG 4 cut(s) 239, 275, 389, 1033
DpnI GATC 4 cut(s) 229, 542, 561, 990
DpnII GATC 4 cut(s) 227, 540, 559, 988
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 4 cut(s) 300, 396, 1401, 1494
EarI CTCTTC 4 cut(s) 300, 396, 1401, 1494
Ecl136II GAGCTC 2 cut(s) 274, 1174
Eco130I CCWWGG 2 cut(s) 1160, 1462
Eco24I GRGCYC 2 cut(s) 276, 1176
Eco31I GGTCTC 1 cut(s) 444
Eco32I GATATC 1 cut(s) 281
Eco47I GGWCC 3 cut(s) 98, 168, 913
Eco53kI GAGCTC 2 cut(s) 274, 1174
Eco57I CTGAAG 1 cut(s) 1394
Eco88I CYCGRG 1 cut(s) 115
Eco91I GGTNACC 1 cut(s) 62
EcoICRI GAGCTC 2 cut(s) 274, 1174
EcoO109I RGGNCCY 3 cut(s) 98, 913, 1226
EcoO65I GGTNACC 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 29
EcoRII CCWGG 3 cut(s) 445, 991, 1318
EcoRV GATATC 1 cut(s) 281
EcoT14I CCWWGG 2 cut(s) 1160, 1462
EcoT38I GRGCYC 2 cut(s) 276, 1176
ErhI CCWWGG 2 cut(s) 1160, 1462
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 9 cut(s) 126, 164, 541, 602, 794, 817, 842, 851, 1190
FaqI GGGAC 2 cut(s) 111, 155
FatI CATG 9 cut(s) 122, 160, 537, 598, 790, 813, 838, 847, 1186
FauI CCCGC 2 cut(s) 77, 1037
Fnu4HI GCNGC 6 cut(s) 260, 554, 683, 827, 1151, 1221
FokI GGATG 5 cut(s) 323, 542, 617, 971, 1143
FriOI GRGCYC 2 cut(s) 276, 1176
Fsp4HI GCNGC 6 cut(s) 260, 554, 683, 827, 1151, 1221
FspBI CTAG 2 cut(s) 411, 1433
GluI GCNGC 6 cut(s) 260, 554, 683, 827, 1151, 1221
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 3 cut(s) 42, 288, 1227
HapII CCGG 1 cut(s) 1108
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 9 cut(s) 126, 164, 541, 602, 794, 817, 842, 851, 1190
HincII GTYRAC 2 cut(s) 184, 408
HindII GTYRAC 2 cut(s) 184, 408
HindIII AAGCTT 2 cut(s) 751, 1360
HinfI GANTC 9 cut(s) 17, 48, 75, 132, 249, 376, 842, 1088, 1478
HpaII CCGG 1 cut(s) 1108
HphI GGTGA 3 cut(s) 74, 1301, 1318
Hpy166II GTNNAC 4 cut(s) 52, 184, 408, 548
Hpy8I GTNNAC 4 cut(s) 52, 184, 408, 548
HpyAV CCTTC 4 cut(s) 101, 481, 520, 896
HpyCH4III ACNGT 3 cut(s) 85, 661, 1333
HpyCH4V TGCA 7 cut(s) 4, 262, 416, 647, 766, 932, 1447
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 826, 1429
HpyF3I CTNAG 4 cut(s) 239, 275, 389, 1033
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 9 cut(s) 126, 164, 541, 602, 794, 817, 842, 851, 1190
KflI GGGWCCC 1 cut(s) 98
Kzo9I GATC 4 cut(s) 227, 540, 559, 988
LmnI GCTCC 4 cut(s) 490, 757, 1217, 1277
Lsp1109I GCAGC 5 cut(s) 246, 540, 813, 1137, 1232
LweI GCATC 6 cut(s) 425, 639, 656, 807, 919, 1289
MaeI CTAG 2 cut(s) 411, 1433
MaeIII GTNAC 4 cut(s) 62, 594, 1066, 1258
MalI GATC 4 cut(s) 229, 542, 561, 990
MboI GATC 4 cut(s) 227, 540, 559, 988
MboII GAAGA 9 cut(s) 314, 317, 413, 642, 645, 881, 1321, 1391, 1418
MfeI CAATTG 2 cut(s) 703, 950
MhlI GDGCHC 3 cut(s) 122, 276, 1176
MlyI GAGTC 4 cut(s) 57, 141, 851, 1097
MmeI TCCRAC 1 cut(s) 109
MroXI GAANNNNTTC 1 cut(s) 941
MseI TTAA 2 cut(s) 590, 1507
MslI CAYNNNNRTG 1 cut(s) 1490
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 1 cut(s) 1108
MspR9I CCNGG 3 cut(s) 447, 993, 1320
MunI CAATTG 2 cut(s) 703, 950
MvaI CCWGG 3 cut(s) 447, 993, 1320
MwoI GCNNNNNNNGC 3 cut(s) 117, 826, 1429
NdeII GATC 4 cut(s) 227, 540, 559, 988
NlaIII CATG 9 cut(s) 126, 164, 541, 602, 794, 817, 842, 851, 1190
NlaIV GGNNCC 5 cut(s) 99, 100, 169, 915, 1324
NmeAIII GCCGAG 3 cut(s) 18, 35, 1195
NmuCI GTSAC 2 cut(s) 62, 594
NspV TTCGAA 1 cut(s) 399
PagI TCATGA 1 cut(s) 838
PdmI GAANNNNTTC 1 cut(s) 941
PfeI GAWTC 5 cut(s) 17, 75, 249, 376, 1478
PfoI TCCNGGA 2 cut(s) 445, 991
PkrI GCNGC 6 cut(s) 261, 555, 684, 828, 1152, 1222
PleI GAGTC 4 cut(s) 56, 140, 850, 1096
PpsI GAGTC 4 cut(s) 56, 140, 850, 1096
PpuMI RGGWCCY 2 cut(s) 98, 913
Psp124BI GAGCTC 2 cut(s) 276, 1176
Psp5II RGGWCCY 2 cut(s) 98, 913
Psp6I CCWGG 3 cut(s) 445, 991, 1318
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 3 cut(s) 445, 991, 1318
PspN4I GGNNCC 5 cut(s) 99, 100, 169, 915, 1324
PspPI GGNCC 4 cut(s) 98, 168, 913, 1226
PspPPI RGGWCCY 2 cut(s) 98, 913
PstNI CAGNNNCTG 1 cut(s) 986
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 2 cut(s) 92, 324
RsaNI GTAC 2 cut(s) 91, 323
RseI CAYNNNNRTG 1 cut(s) 1490
SacI GAGCTC 2 cut(s) 276, 1176
SaqAI TTAA 2 cut(s) 590, 1507
SatI GCNGC 6 cut(s) 260, 554, 683, 827, 1151, 1221
Sau3AI GATC 4 cut(s) 227, 540, 559, 988
Sau96I GGNCC 4 cut(s) 98, 168, 913, 1226
SchI GAGTC 4 cut(s) 57, 141, 851, 1097
ScrFI CCNGG 3 cut(s) 447, 993, 1320
SduI GDGCHC 3 cut(s) 122, 276, 1176
SfaNI GCATC 6 cut(s) 425, 639, 656, 807, 919, 1289
SfcI CTRYAG 1 cut(s) 1242
SfuI TTCGAA 1 cut(s) 399
SinI GGWCC 3 cut(s) 98, 168, 913
SmiMI CAYNNNNRTG 1 cut(s) 1490
SmlI CTYRAG 4 cut(s) 269, 297, 821, 1175
SmoI CTYRAG 4 cut(s) 269, 297, 821, 1175
SpeI ACTAGT 1 cut(s) 410
SsiI CCGC 3 cut(s) 70, 682, 1030
SspI AATATT 1 cut(s) 1135
SspMI CTAG 2 cut(s) 411, 1433
SstI GAGCTC 2 cut(s) 276, 1176
StyD4I CCNGG 3 cut(s) 445, 991, 1318
StyI CCWWGG 2 cut(s) 1160, 1462
TaaI ACNGT 3 cut(s) 85, 661, 1333
TaqI TCGA 4 cut(s) 135, 226, 283, 399
TatI WGTACW 1 cut(s) 322
TauI GCSGC 1 cut(s) 685
TfiI GAWTC 5 cut(s) 17, 75, 249, 376, 1478
Tru1I TTAA 2 cut(s) 590, 1507
Tru9I TTAA 2 cut(s) 590, 1507
TscAI CASTG 3 cut(s) 90, 939, 987
TseFI GTSAC 2 cut(s) 62, 594
TseI GCWGC 5 cut(s) 259, 553, 826, 1150, 1220
Tsp45I GTSAC 2 cut(s) 62, 594
TspDTI ATGAA 8 cut(s) 327, 738, 864, 1145, 1175, 1397, 1455, 1491
TspGWI ACGGA 2 cut(s) 160, 623
TspRI CASTG 3 cut(s) 90, 939, 987
VpaK11BI GGWCC 3 cut(s) 98, 168, 913
XapI RAATTY 6 cut(s) 29, 481, 691, 874, 1095, 1347
XmnI GAANNNNTTC 1 cut(s) 941
XspI CTAG 2 cut(s) 411, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.