Rh7BG397700

Tubulin-specific chaperone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004433
Physical Location & Seq
Forward (+)
44113616 .. 44121111
7496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG397700.1

Sequence Viewer

Length: 1617 bp
ATGCAACCCTCATCGGAATCAAACCCAGCCTTCAAACTCGGCCAGCGAGTCCACTCCGCCGGTGATCCCCGCCGAATCGGAACAGTGAGGTACTTGGGACCCGTTGAAGGCTACTCGGGCACATGGGTCGGAGTCGATTGGGACAACAGAGACGGCAAGCATGATGGGTCCGTCAATGGCGTCAACTACTTCCACGCAAGGTCTGAAACATCTGGGTCCTTTGTTCGACCCCACAATCTCAGCTCTGGAATCTCATTGTTGCAAGCTCTTGAGCTCAGATATCGAGGCCAGACCACTCGAGAAGAAGAGGATGAAATGTATGTTCTTTCTGCGAGCAACAAGCGTGTGAGTGTTCAACTTGTTGGTAAAGACAAGATTCAAGACAAGCTGAGTTGCTTCGAAGAGTTGACTAGTGCATCAACATCTTACTTGGGTGTTAGCTCTCCTGGAGACCCATCTGAAATTGGTGCTGTTGTGCCAAAATTGAAGGAGCTTGACTTGACTGGAAATCTAATTTCAGAGTGGAAGGACGTTGGCACAATCTGTGAACAGCTGCCTGATCTTGTTGCCCTCAATTTATCGTACAATTTAATGTCACATGATATTGTTGGGTTGCCGCATTTGAAAAGCATCCGTATTTTAGTATTAAACAATGTTGGCATAAATTGGACACAGGTAGAAGTACTCAAACATTCATTGCCAGCAATTGGGGAACTACATCTTATGGCCAATCATATAAGCACAATAGAGCCTGCATCTTCTTCAACAGTTCAGGGATTTGATTATTTGCGGCTACTGAATTTGGACGACAATTGTATAACTGATTGGAATGAAATCTTGAAACTTTCACAGCTACCAAGCTTGGAGCAGTTGCATTTGGACAATAACAATTTGAACCATGTCTTTTATCCTGATGTTGGCATGATGCTTGAGCTGCTCAATGGTCATGAGTCGCATGAAAAAGGGCTTAAGCCATTTCAAAATTTGCGTTGCCTTCTTCTTGGAGGCAATACCATTGAGGACCCTAACTCTATTGATGCACTGAACTCATTCCCACAATTGGTGGATGTCAGACTTTCTGGGAATCCAGTGGCTGATCCAGGACAGGGTGGTATCCCAAGATTTGTTTTGGTTGCCCGCTTAGGAAAAGTTGAAATGTTGAATGGGAGTGAGGTTACTTCTCGTGAAAGAAAAGAGTCCGAAATTCGGTATGTCCGGTTAGTTATGTCAAAGCAAGGAAATATTCAAGAAATCAAGCTGCTTCATCCGAGGTTTGCCGAGCTCAAGGCTTTTCATGGAATTGAGGACGAAAAGCCATTGGTGGGAGCAGCAGGCCCTCAAAAGATGGCTACAGGGCTTCTATCTGTTACTCTGAAATGCGTGGGAGCATCTATAGGTGAGAAGCCCTCTTTGGTGAAGAAACTTCCAGGCACCACAACGGTTGGTAAGCTAAAAATTCTTTGTGAAAGCTTTTTCAAGCTGAAGTCTATGAAGTTGAAATTGTTTCTTCAAGAAGAGGGTTCTCCTTTGCCAATGTTGCTTGATGATGAAATGCAGAGTTTGACAGACCTTGGGATTGGCAATGAATCAACCATTCTTGTGGATGAAGAGAGTTAA

Protein Analysis

538

Amino Acids

59.18

Weight (kDa)

5.53

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP_GLY PF01302 13 - 79 3.2e-22 CAP-Gly domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1430
AccB7I CCANNNNNTGG 1 cut(s) 707
AciI CCGC 5 cut(s) 57, 70, 617, 790, 1138
AclWI GGATC 2 cut(s) 59, 1091
AcoI YGGCCR 2 cut(s) 40, 726
AcsI RAATTY 4 cut(s) 799, 982, 1203, 1455
AcuI CTGAAG 1 cut(s) 1502
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 92, 584, 684
AfiI CCNNNNNNNGG 7 cut(s) 107, 707, 917, 1060, 1106, 1206, 1322
AflII CTTAAG 1 cut(s) 968
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 3 cut(s) 445, 1099, 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 1394, 1426
Alw21I GWGCWC 2 cut(s) 276, 1284
Alw26I GTCTC 2 cut(s) 144, 444
AlwI GGATC 2 cut(s) 59, 1091
AlwNI CAGNNNCTG 1 cut(s) 1094
Ama87I CYCGRG 2 cut(s) 115, 297
AoxI GGCC 4 cut(s) 40, 286, 726, 1333
ApeKI GCWGC 4 cut(s) 553, 934, 1258, 1328
ApoI RAATTY 4 cut(s) 799, 982, 1203, 1455
Asp700I GAANNNNTTC 1 cut(s) 1049
AspS9I GGNCC 5 cut(s) 98, 168, 216, 1021, 1334
AsuHPI GGTGA 3 cut(s) 74, 1409, 1426
AsuII TTCGAA 1 cut(s) 399
AvaI CYCGRG 2 cut(s) 115, 297
AvaII GGWCC 4 cut(s) 98, 168, 216, 1021
BaeGI GKGCMC 1 cut(s) 122
BalI TGGCCA 1 cut(s) 728
BanI GGYRCC 1 cut(s) 1430
BanII GRGCYC 2 cut(s) 276, 1284
BauI CACGAG 1 cut(s) 1182
Bbv12I GWGCWC 2 cut(s) 276, 1284
BbvI GCAGC 4 cut(s) 540, 921, 1245, 1340
BccI CCATC 3 cut(s) 158, 463, 1339
BceAI ACGGC 1 cut(s) 169
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 3 cut(s) 447, 1101, 1428
BciVI GTATCC 1 cut(s) 1124
BcoDI GTCTC 2 cut(s) 144, 444
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 2 cut(s) 1350, 1392
BfrI CTTAAG 1 cut(s) 968
BfuI GTATCC 1 cut(s) 1124
BisI GCNGC 6 cut(s) 554, 617, 791, 935, 1259, 1329
BlsI GCNGC 6 cut(s) 555, 618, 792, 936, 1260, 1330
BmcAI AGTACT 1 cut(s) 684
Bme1390I CCNGG 3 cut(s) 447, 1101, 1428
Bme18I GGWCC 4 cut(s) 98, 168, 216, 1021
BmeT110I CYCGRG 2 cut(s) 115, 297
BmgT120I GGNCC 5 cut(s) 98, 168, 216, 1021, 1334
BmiI GGNNCC 6 cut(s) 99, 100, 169, 217, 1023, 1432
BmrFI CCNGG 3 cut(s) 447, 1101, 1428
BmsI GCATC 6 cut(s) 425, 639, 764, 915, 1027, 1397
BplI GAGNNNNNCTC 2 cut(s) 1391, 1423
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 1 cut(s) 1141
Bpu14I TTCGAA 1 cut(s) 399
BpuEI CTTGAG 3 cut(s) 290, 950, 1268
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 2 cut(s) 1268, 1570
BsaWI WCCGGW 1 cut(s) 1215
Bsc4I CCNNNNNNNGG 7 cut(s) 107, 707, 917, 1060, 1106, 1206, 1322
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 508, 1088
Bse3DI GCAATG 2 cut(s) 695, 1588
BseBI CCWGG 3 cut(s) 447, 1101, 1428
BseDI CCNNGG 2 cut(s) 1268, 1570
BseGI GGATG 5 cut(s) 316, 630, 1072, 1264, 1609
BseLI CCNNNNNNNGG 7 cut(s) 107, 707, 917, 1060, 1106, 1206, 1322
BseMI GCAATG 2 cut(s) 695, 1588
BseMII CTCAG 3 cut(s) 253, 289, 380
BseNI ACTGG 2 cut(s) 508, 1088
BseSI GKGCMC 1 cut(s) 122
BseXI GCAGC 4 cut(s) 540, 921, 1245, 1340
BseYI CCCAGC 1 cut(s) 25
BshFI GGCC 4 cut(s) 42, 288, 728, 1335
BshNI GGYRCC 1 cut(s) 1430
BsiHKAI GWGCWC 2 cut(s) 276, 1284
BsiHKCI CYCGRG 2 cut(s) 115, 297
BsiSI CCGG 2 cut(s) 60, 1216
BslFI GGGAC 2 cut(s) 111, 155
BslI CCNNNNNNNGG 7 cut(s) 107, 707, 917, 1060, 1106, 1206, 1322
BsmAI GTCTC 2 cut(s) 144, 444
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 2 cut(s) 111, 155
BsnI GGCC 4 cut(s) 42, 288, 728, 1335
Bso31I GGTCTC 1 cut(s) 444
BsoBI CYCGRG 2 cut(s) 115, 297
Bsp119I TTCGAA 1 cut(s) 399
Bsp1286I GDGCHC 3 cut(s) 122, 276, 1284
Bsp143I GATC 3 cut(s) 64, 559, 1096
BspACI CCGC 5 cut(s) 57, 70, 617, 790, 1138
BspANI GGCC 4 cut(s) 42, 288, 728, 1335
BspCNI CTCAG 3 cut(s) 252, 288, 381
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 6 cut(s) 99, 100, 169, 217, 1023, 1432
BspPI GGATC 2 cut(s) 59, 1091
BspT104I TTCGAA 1 cut(s) 399
BspT107I GGYRCC 1 cut(s) 1430
BspTI CTTAAG 1 cut(s) 968
BspTNI GGTCTC 1 cut(s) 444
BsrDI GCAATG 2 cut(s) 695, 1588
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 2 cut(s) 508, 1088
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 2 cut(s) 1268, 1570
BssMI GATC 3 cut(s) 64, 559, 1096
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 1182
BssT1I CCWWGG 1 cut(s) 1570
Bst2BI CACGAG 1 cut(s) 1182
Bst2UI CCWGG 3 cut(s) 447, 1101, 1428
Bst4CI ACNGT 3 cut(s) 85, 769, 1441
Bst6I CTCTTC 4 cut(s) 300, 396, 1509, 1602
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 399
BstC8I GCNNGC 8 cut(s) 44, 158, 264, 334, 702, 753, 1138, 1333
BstDEI CTNAG 4 cut(s) 239, 275, 389, 1141
BstF5I GGATG 5 cut(s) 316, 630, 1072, 1264, 1609
BstKTI GATC 3 cut(s) 67, 562, 1099
BstMAI GTCTC 2 cut(s) 144, 444
BstMBI GATC 3 cut(s) 64, 559, 1096
BstMWI GCNNNNNNNGC 3 cut(s) 117, 934, 1537
BstNI CCWGG 3 cut(s) 447, 1101, 1428
BstSCI CCNGG 3 cut(s) 445, 1099, 1426
BstSFI CTRYAG 2 cut(s) 1350, 1392
BstSLI GKGCMC 1 cut(s) 122
BstV1I GCAGC 4 cut(s) 540, 921, 1245, 1340
BstXI CCANNNNNNTGG 1 cut(s) 1600
BsuI GTATCC 1 cut(s) 1124
BsuRI GGCC 4 cut(s) 42, 288, 728, 1335
BtsCI GGATG 5 cut(s) 316, 630, 1072, 1264, 1609
BtsIMutI CAGTG 3 cut(s) 90, 1040, 1095
Cac8I GCNNGC 8 cut(s) 44, 158, 264, 334, 702, 753, 1138, 1333
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 1 cut(s) 946
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 5 cut(s) 98, 168, 216, 1021, 1334
CseI GACGC 1 cut(s) 169
Csp6I GTAC 3 cut(s) 91, 583, 683
CspCI CAANNNNNGTGG 2 cut(s) 1423, 1458
CviAII CATG 8 cut(s) 123, 161, 599, 899, 922, 947, 956, 1295
CviQI GTAC 3 cut(s) 91, 583, 683
DdeI CTNAG 4 cut(s) 239, 275, 389, 1141
DpnI GATC 3 cut(s) 66, 561, 1098
DpnII GATC 3 cut(s) 64, 559, 1096
EaeI YGGCCR 2 cut(s) 40, 726
Eam1104I CTCTTC 4 cut(s) 300, 396, 1509, 1602
EarI CTCTTC 4 cut(s) 300, 396, 1509, 1602
EciI GGCGGA 1 cut(s) 46
Ecl136II GAGCTC 2 cut(s) 274, 1282
Eco130I CCWWGG 1 cut(s) 1570
Eco24I GRGCYC 2 cut(s) 276, 1284
Eco31I GGTCTC 1 cut(s) 444
Eco32I GATATC 1 cut(s) 281
Eco47I GGWCC 4 cut(s) 98, 168, 216, 1021
Eco53kI GAGCTC 2 cut(s) 274, 1282
Eco57I CTGAAG 1 cut(s) 1502
Eco88I CYCGRG 2 cut(s) 115, 297
EcoICRI GAGCTC 2 cut(s) 274, 1282
EcoO109I RGGNCCY 4 cut(s) 98, 216, 1021, 1334
EcoRII CCWGG 3 cut(s) 445, 1099, 1426
EcoRV GATATC 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 1570
EcoT38I GRGCYC 2 cut(s) 276, 1284
ErhI CCWWGG 1 cut(s) 1570
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 8 cut(s) 126, 164, 602, 902, 925, 950, 959, 1298
FaqI GGGAC 2 cut(s) 111, 155
FatI CATG 8 cut(s) 122, 160, 598, 898, 921, 946, 955, 1294
FauI CCCGC 2 cut(s) 77, 1145
Fnu4HI GCNGC 6 cut(s) 554, 617, 791, 935, 1259, 1329
FokI GGATG 4 cut(s) 323, 617, 1079, 1251
FriOI GRGCYC 2 cut(s) 276, 1284
Fsp4HI GCNGC 6 cut(s) 554, 617, 791, 935, 1259, 1329
FspBI CTAG 1 cut(s) 411
GluI GCNGC 6 cut(s) 554, 617, 791, 935, 1259, 1329
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 4 cut(s) 42, 288, 728, 1335
HapII CCGG 2 cut(s) 60, 1216
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 8 cut(s) 126, 164, 602, 902, 925, 950, 959, 1298
HincII GTYRAC 2 cut(s) 184, 408
HindII GTYRAC 2 cut(s) 184, 408
HindIII AAGCTT 2 cut(s) 859, 1468
HpaII CCGG 2 cut(s) 60, 1216
HphI GGTGA 3 cut(s) 74, 1409, 1426
Hpy166II GTNNAC 4 cut(s) 52, 184, 408, 548
Hpy8I GTNNAC 4 cut(s) 52, 184, 408, 548
HpyAV CCTTC 5 cut(s) 40, 101, 481, 520, 1004
HpyCH4III ACNGT 3 cut(s) 85, 769, 1441
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 7 cut(s) 4, 262, 416, 755, 874, 1040, 1555
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 934, 1537
HpyF3I CTNAG 4 cut(s) 239, 275, 389, 1141
HpySE526I ACGT 1 cut(s) 531
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 8 cut(s) 126, 164, 602, 902, 925, 950, 959, 1298
KflI GGGWCCC 1 cut(s) 98
Kzo9I GATC 3 cut(s) 64, 559, 1096
LmnI GCTCC 4 cut(s) 490, 865, 1325, 1385
Lsp1109I GCAGC 4 cut(s) 540, 921, 1245, 1340
LweI GCATC 6 cut(s) 425, 639, 764, 915, 1027, 1397
MaeI CTAG 1 cut(s) 411
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 3 cut(s) 594, 1174, 1366
MalI GATC 3 cut(s) 66, 561, 1098
MboI GATC 3 cut(s) 64, 559, 1096
MboII GAAGA 9 cut(s) 314, 317, 413, 750, 753, 989, 1429, 1499, 1526
MfeI CAATTG 3 cut(s) 705, 811, 1058
MhlI GDGCHC 3 cut(s) 122, 276, 1284
MlsI TGGCCA 1 cut(s) 728
MluNI TGGCCA 1 cut(s) 728
MlyI GAGTC 4 cut(s) 57, 141, 959, 1205
MmeI TCCRAC 1 cut(s) 109
Mox20I TGGCCA 1 cut(s) 728
MroXI GAANNNNTTC 1 cut(s) 1049
MscI TGGCCA 1 cut(s) 728
MseI TTAA 4 cut(s) 590, 647, 969, 1615
MslI CAYNNNNRTG 1 cut(s) 1598
Msp20I TGGCCA 1 cut(s) 728
MspA1I CMGCKG 1 cut(s) 553
MspCI CTTAAG 1 cut(s) 968
MspI CCGG 2 cut(s) 60, 1216
MspR9I CCNGG 3 cut(s) 447, 1101, 1428
MunI CAATTG 3 cut(s) 705, 811, 1058
MvaI CCWGG 3 cut(s) 447, 1101, 1428
MwoI GCNNNNNNNGC 3 cut(s) 117, 934, 1537
NdeII GATC 3 cut(s) 64, 559, 1096
NlaIII CATG 8 cut(s) 126, 164, 602, 902, 925, 950, 959, 1298
NlaIV GGNNCC 6 cut(s) 99, 100, 169, 217, 1023, 1432
NmeAIII GCCGAG 2 cut(s) 18, 1303
NmuCI GTSAC 1 cut(s) 594
NspV TTCGAA 1 cut(s) 399
PaeR7I CTCGAG 1 cut(s) 297
PagI TCATGA 1 cut(s) 946
PdmI GAANNNNTTC 1 cut(s) 1049
PfeI GAWTC 6 cut(s) 17, 75, 249, 376, 1084, 1586
PflMI CCANNNNNTGG 1 cut(s) 707
PfoI TCCNGGA 2 cut(s) 445, 1099
PkrI GCNGC 6 cut(s) 555, 618, 792, 936, 1260, 1330
PleI GAGTC 4 cut(s) 56, 140, 958, 1204
PpsI GAGTC 4 cut(s) 56, 140, 958, 1204
PpuMI RGGWCCY 3 cut(s) 98, 216, 1021
Psp124BI GAGCTC 2 cut(s) 276, 1284
Psp5II RGGWCCY 3 cut(s) 98, 216, 1021
Psp6I CCWGG 3 cut(s) 445, 1099, 1426
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 3 cut(s) 445, 1099, 1426
PspN4I GGNNCC 6 cut(s) 99, 100, 169, 217, 1023, 1432
PspPI GGNCC 5 cut(s) 98, 168, 216, 1021, 1334
PspPPI RGGWCCY 3 cut(s) 98, 216, 1021
PstNI CAGNNNCTG 1 cut(s) 1094
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 3 cut(s) 92, 584, 684
RsaNI GTAC 3 cut(s) 91, 583, 683
RseI CAYNNNNRTG 1 cut(s) 1598
SacI GAGCTC 2 cut(s) 276, 1284
SaqAI TTAA 4 cut(s) 590, 647, 969, 1615
SatI GCNGC 6 cut(s) 554, 617, 791, 935, 1259, 1329
Sau3AI GATC 3 cut(s) 64, 559, 1096
Sau96I GGNCC 5 cut(s) 98, 168, 216, 1021, 1334
ScaI AGTACT 1 cut(s) 684
SchI GAGTC 4 cut(s) 57, 141, 959, 1205
ScrFI CCNGG 3 cut(s) 447, 1101, 1428
SduI GDGCHC 3 cut(s) 122, 276, 1284
SfaNI GCATC 6 cut(s) 425, 639, 764, 915, 1027, 1397
SfcI CTRYAG 2 cut(s) 1350, 1392
Sfr274I CTCGAG 1 cut(s) 297
SfuI TTCGAA 1 cut(s) 399
SgrAI CRCCGGYG 1 cut(s) 59
SinI GGWCC 4 cut(s) 98, 168, 216, 1021
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 1598
SmlI CTYRAG 5 cut(s) 269, 297, 929, 968, 1283
SmoI CTYRAG 5 cut(s) 269, 297, 929, 968, 1283
SpeI ACTAGT 1 cut(s) 410
SsiI CCGC 5 cut(s) 57, 70, 617, 790, 1138
SspI AATATT 1 cut(s) 1243
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 2 cut(s) 276, 1284
StyD4I CCNGG 3 cut(s) 445, 1099, 1426
StyI CCWWGG 1 cut(s) 1570
TaaI ACNGT 3 cut(s) 85, 769, 1441
TaiI ACGT 1 cut(s) 534
TaqI TCGA 5 cut(s) 135, 226, 283, 298, 399
TatI WGTACW 1 cut(s) 682
TauI GCSGC 2 cut(s) 619, 793
TfiI GAWTC 6 cut(s) 17, 75, 249, 376, 1084, 1586
Tru1I TTAA 4 cut(s) 590, 647, 969, 1615
Tru9I TTAA 4 cut(s) 590, 647, 969, 1615
TscAI CASTG 3 cut(s) 90, 1047, 1095
TseFI GTSAC 1 cut(s) 594
TseI GCWGC 4 cut(s) 553, 934, 1258, 1328
Tsp45I GTSAC 1 cut(s) 594
TspDTI ATGAA 9 cut(s) 327, 684, 846, 972, 1253, 1283, 1505, 1563, 1599
TspGWI ACGGA 2 cut(s) 160, 623
TspRI CASTG 3 cut(s) 90, 1047, 1095
Van91I CCANNNNNTGG 1 cut(s) 707
Vha464I CTTAAG 1 cut(s) 968
VpaK11BI GGWCC 4 cut(s) 98, 168, 216, 1021
XapI RAATTY 4 cut(s) 799, 982, 1203, 1455
XhoI CTCGAG 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 1049
XspI CTAG 1 cut(s) 411
ZrmI AGTACT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.