Rmu_sc0000711.1_g000029

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000711.1
OG0022728
Physical Location & Seq
Forward (+)
155763 .. 156428
666 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
atgtccagacattgctctgaatccagtgttattcatgaaattgttgaagagatttcagagcaagtgataagcagtacatatttggatgtggctaagtacccagtaggaataaaacctcgggtgcaacagatccgtaaccttttaggtgtcaggggaagaggtgttcgtatggtagggatatgggggcccggaggaataggcaagacaacaattgccaaagctgtctataactcaattgctcataaatttgatgggagttgttttttggaaaatgttagagaaaattcaatgggtgccaaaggctttgtcaaactacaaaagaaacttctttttgagattctaaagggaacaaaattgaaggtgaccaatgtagcaagaggaatcactatgattaaggaaaggttgcaatacaaaagtgttctcttagttcttgatgatgtgaatagcatggatcagatagataacttagctggtcaatgtagttggtttggaatgggtagtagaatcatcataacaacaagggataagcaattgctgaggcatcataatgttaataacttaatatacgaggtccagaaattggatcatcatgaagctctcgagctattaagtatgaatgcctttaaaagaagtcgaactttggatgcttatgcagaactcacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

221

Amino Acids

24.9

Weight (kDa)

9.51

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 41 - 211 4.5e-21 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022728)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0411741
rosa_multiflora Rmu_co8266463.1_g000001 Rmu_sc0000711.1_g000029
rosa_roxburghii Rroxscaffold_5G00358000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 580
AclWI GGATC 3 cut(s) 124, 459, 591
AcsI RAATTY 2 cut(s) 245, 283
AfaI GTAC 2 cut(s) 76, 98
AfiI CCNNNNNNNGG 1 cut(s) 580
AgsI TTSAA 3 cut(s) 47, 288, 358
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 4 cut(s) 221, 470, 596, 604
AluI AGCT 4 cut(s) 221, 470, 596, 604
AlwI GGATC 3 cut(s) 124, 459, 591
Ama87I CYCGRG 2 cut(s) 117, 599
AoxI GGCC 1 cut(s) 185
ApaI GGGCCC 1 cut(s) 189
ApoI RAATTY 2 cut(s) 245, 283
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AspS9I GGNCC 3 cut(s) 185, 186, 571
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 373
AvaI CYCGRG 2 cut(s) 117, 599
AvaII GGWCC 1 cut(s) 571
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 536
BccI CCATC 1 cut(s) 245
BcnI CCSGG 1 cut(s) 189
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 571
BmeT110I CYCGRG 2 cut(s) 117, 599
BmgT120I GGNCC 3 cut(s) 185, 186, 571
BmiI GGNNCC 3 cut(s) 186, 187, 295
BmrFI CCNGG 1 cut(s) 189
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 2 cut(s) 550, 634
BmuI ACTGGG 1 cut(s) 95
Bpu10I CCTNAGC 1 cut(s) 536
BpuMI CCSGG 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 116
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse1I ACTGG 2 cut(s) 24, 101
Bse3DI GCAATG 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 2 cut(s) 91, 649
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMI GCAATG 1 cut(s) 10
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 2 cut(s) 24, 101
BseSI GKGCMC 1 cut(s) 189
BshFI GGCC 1 cut(s) 187
BshNI GGYRCC 1 cut(s) 293
BsiHKCI CYCGRG 2 cut(s) 117, 599
BsiSI CCGG 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 580
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 1 cut(s) 187
BsoBI CYCGRG 2 cut(s) 117, 599
Bsp120I GGGCCC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 3 cut(s) 129, 451, 583
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 528
BspHI TCATGA 2 cut(s) 34, 589
BspLI GGNNCC 3 cut(s) 186, 187, 295
BspPI GGATC 3 cut(s) 124, 459, 591
BspT107I GGYRCC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 10
BsrI ACTGG 2 cut(s) 24, 101
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 3 cut(s) 129, 451, 583
Bst6I CTCTTC 2 cut(s) 42, 151
BstDEI CTNAG 4 cut(s) 93, 424, 466, 536
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 2 cut(s) 91, 649
BstKTI GATC 3 cut(s) 132, 454, 586
BstMBI GATC 3 cut(s) 129, 451, 583
BstPI GGTNACC 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BsuRI GGCC 1 cut(s) 187
BtsCI GGATG 2 cut(s) 91, 649
BtsIMutI CAGTG 1 cut(s) 31
CciI TCATGA 2 cut(s) 34, 589
Cfr13I GGNCC 3 cut(s) 185, 186, 571
Csp6I GTAC 2 cut(s) 75, 97
CviAII CATG 3 cut(s) 35, 448, 590
CviJI RGCY 7 cut(s) 92, 187, 221, 303, 470, 596, 604
CviKI_1 RGCY 7 cut(s) 92, 187, 221, 303, 470, 596, 604
CviQI GTAC 2 cut(s) 75, 97
DdeI CTNAG 4 cut(s) 93, 424, 466, 536
DpnI GATC 3 cut(s) 131, 453, 585
DpnII GATC 3 cut(s) 129, 451, 583
DraI TTTAAA 1 cut(s) 625
Eam1104I CTCTTC 2 cut(s) 42, 151
EarI CTCTTC 2 cut(s) 42, 151
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 571
Eco88I CYCGRG 2 cut(s) 117, 599
Eco91I GGTNACC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 185
EcoO65I GGTNACC 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 3 cut(s) 38, 451, 593
FalI AAGNNNNNCTT 2 cut(s) 622, 654
FatI CATG 3 cut(s) 34, 447, 589
FokI GGATG 2 cut(s) 98, 656
FriOI GRGCYC 1 cut(s) 189
HaeIII GGCC 1 cut(s) 187
HapII CCGG 1 cut(s) 189
Hin1II CATG 3 cut(s) 38, 451, 593
HinfI GANTC 4 cut(s) 20, 337, 381, 504
HpaII CCGG 1 cut(s) 189
HphI GGTGA 1 cut(s) 373
Hpy188I TCNGA 3 cut(s) 19, 58, 456
Hpy188III TCNNGA 6 cut(s) 6, 35, 431, 574, 590, 599
HpyAV CCTTC 1 cut(s) 352
HpyCH4V TGCA 3 cut(s) 124, 406, 653
HpyF3I CTNAG 4 cut(s) 93, 424, 466, 536
Hsp92II CATG 3 cut(s) 38, 451, 593
Kzo9I GATC 3 cut(s) 129, 451, 583
LpnPI CCDG 7 cut(s) 19, 37, 114, 136, 202, 456, 587
LweI GCATC 2 cut(s) 550, 634
MaeIII GTNAC 2 cut(s) 134, 361
MalI GATC 3 cut(s) 131, 453, 585
MboI GATC 3 cut(s) 129, 451, 583
MboII GAAGA 2 cut(s) 59, 168
MfeI CAATTG 3 cut(s) 210, 234, 530
MflI RGATCY 1 cut(s) 129
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 8 cut(s) 39, 210, 234, 245, 283, 353, 530, 578
MnlI CCTC 6 cut(s) 126, 152, 185, 371, 531, 562
MseI TTAA 5 cut(s) 393, 552, 560, 608, 624
MslI CAYNNNNRTG 1 cut(s) 546
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 3 cut(s) 210, 234, 530
Mva1269I GAATGC 1 cut(s) 622
NciI CCSGG 1 cut(s) 189
NdeII GATC 3 cut(s) 129, 451, 583
NlaIII CATG 3 cut(s) 38, 451, 593
NlaIV GGNNCC 3 cut(s) 186, 187, 295
NmuCI GTSAC 1 cut(s) 361
PaeR7I CTCGAG 1 cut(s) 599
PagI TCATGA 2 cut(s) 34, 589
PctI GAATGC 1 cut(s) 622
PfeI GAWTC 4 cut(s) 20, 337, 381, 504
PflMI CCANNNNNTGG 1 cut(s) 580
PspEI GGTNACC 1 cut(s) 361
PspN4I GGNNCC 3 cut(s) 186, 187, 295
PspOMI GGGCCC 1 cut(s) 185
PspPI GGNCC 3 cut(s) 185, 186, 571
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 2 cut(s) 76, 98
RsaNI GTAC 2 cut(s) 75, 97
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 5 cut(s) 393, 552, 560, 608, 624
Sau3AI GATC 3 cut(s) 129, 451, 583
Sau96I GGNCC 3 cut(s) 185, 186, 571
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 189
SfaNI GCATC 2 cut(s) 550, 634
Sfr274I CTCGAG 1 cut(s) 599
SinI GGWCC 1 cut(s) 571
SlaI CTCGAG 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse9I AATT 8 cut(s) 39, 210, 234, 245, 283, 353, 530, 578
StyD4I CCNGG 1 cut(s) 187
TaqI TCGA 2 cut(s) 600, 634
TasI AATT 8 cut(s) 39, 210, 234, 245, 283, 353, 530, 578
TatI WGTACW 1 cut(s) 74
TfiI GAWTC 4 cut(s) 20, 337, 381, 504
Tru1I TTAA 5 cut(s) 393, 552, 560, 608, 624
Tru9I TTAA 5 cut(s) 393, 552, 560, 608, 624
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 1 cut(s) 361
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 4 cut(s) 23, 51, 606, 629
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 1 cut(s) 31
Van91I CCANNNNNTGG 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 571
XapI RAATTY 2 cut(s) 245, 283
XhoI CTCGAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.