Rmu_sc0000735.1_g000006

Nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000735.1
OG0002055
Physical Location & Seq
Reverse (-)
13041 .. 16492
3452 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1533 bp
atgttccattgtttaaccaaattgtgggccattcctgattcagccgaggccattcttcttgggtttcaacattatcttgtgatgcttggcacttcagttctcattcctagtgctcttgttccccagatgggaggtggaaatgaggagaaggccaaggtgatccagacgctactctttgttgctggtttgaacaccttgaagcaaacattgtttgggactagactacctgcagtgattggagggtcatacacgtttgttcccaccacagtttcaatcatattagctagtcgattcagtgattctcaagaccctatagacaaatttaagagaacaatgcgggcaattcaaggagctcttattgtggcctcaacccttcagatagtcttaggcttcagtggcctttggcgtaatgttgcaaggtttttaagtccactgtcaattgttccattgataggactagttggttttggactctacgagttaggttttcctctgcttgccaaatgtgttgagattggactgccagagcttatcattttagtatttgtctctcagtatttgccccacatatttcacagaggaaaacatgtatttgatcgttttgctgttctatttgccattgtgatcgtgtggatttatgctcatctgctcactgttggcggggcatataatgattcaggccccaaaacacaagcaagttgccgtactgaccgtgctggacttatagatggtgctccatggataagagttccgtatcccttccaatggggagcacctacatttgatgctggtgaagcctttgctatgatgactgcgtcttttgttgctctcgtagagtccactggtgctttcattgctgtatcaaggtatgctagtgcaactccagtgccaccttctattctgagtcgtggggttggttggcagggtgttgggattctgatttccgggctatttggaactgtgaccggatcctcagtatctgtagagaatgctggtcttttggcgttaacacgtgttggcagtcgaagggttgtgcaaatatctgctgggttcatgatatttttctccattctgggcaaatttggagcagtcttcgcttcaatcccagggcccatcattgctgctttatattgtctgttctttgcttatgtcggttcggggggtcttagctttctccagttctgcaacctgaacagctttcgaaccaagttcattttaggcttctctatcttcatgggtttgtctataccgcagtacttcaatgagtacaccctcctcaacggttatggtccagtccacacaggaggaagatggtttaatgatatgatcaatgtccctttccaatcggaagcgtttgtggcgggcattattgcatatttcttggacaacacgctgcatcgaaatgacatcacagttaggaaggacaggggtaaacattggtgggacaagttccggtcattcaagggtgattcaaggagtgaggagttctattcattgccgttcaatctaaacaagtattttccatctgtatga

Protein Analysis

510

Amino Acids

55.81

Weight (kDa)

9.4

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 16 - 420 1.5e-66 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AccB7I CCANNNNNTGG 1 cut(s) 24
AciI CCGC 4 cut(s) 337, 660, 1250, 1361
AclWI GGATC 3 cut(s) 154, 963, 976
AcsI RAATTY 2 cut(s) 320, 1079
AcuI CTGAAG 3 cut(s) 78, 359, 376
AcvI CACGTG 1 cut(s) 1013
AfaI GTAC 3 cut(s) 706, 1256, 1268
AfiI CCNNNNNNNGG 4 cut(s) 24, 128, 452, 765
AflIII ACRYGT 4 cut(s) 249, 586, 1010, 1012
AhlI ACTAGT 1 cut(s) 457
AjnI CCWGG 1 cut(s) 1105
AluBI AGCT 5 cut(s) 284, 353, 529, 1170, 1197
AluI AGCT 5 cut(s) 284, 353, 529, 1170, 1197
Alw21I GWGCWC 4 cut(s) 115, 355, 736, 775
Alw26I GTCTC 1 cut(s) 553
AlwI GGATC 3 cut(s) 154, 963, 976
AlwNI CAGNNNCTG 1 cut(s) 980
AoxI GGCC 7 cut(s) 27, 48, 150, 363, 397, 679, 1109
ApaI GGGCCC 1 cut(s) 1113
ApeKI GCWGC 2 cut(s) 1121, 1393
ApoI RAATTY 2 cut(s) 320, 1079
AspS9I GGNCC 5 cut(s) 27, 680, 1109, 1110, 1289
AsuC2I CCSGG 1 cut(s) 946
AsuHPI GGTGA 3 cut(s) 169, 803, 1478
AsuII TTCGAA 1 cut(s) 1201
AvaII GGWCC 1 cut(s) 1289
BaeGI GKGCMC 1 cut(s) 1113
BamHI GGATCC 1 cut(s) 968
BanII GRGCYC 2 cut(s) 355, 1113
BbrPI CACGTG 1 cut(s) 1013
BbsI GAAGAC 1 cut(s) 1084
Bbv12I GWGCWC 4 cut(s) 115, 355, 736, 775
BbvI GCAGC 2 cut(s) 1108, 1380
BccI CCATC 5 cut(s) 121, 722, 1121, 1305, 1531
BceAI ACGGC 2 cut(s) 687, 1483
BciT130I CCWGG 1 cut(s) 1107
BciVI GTATCC 1 cut(s) 765
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 1 cut(s) 946
BcoDI GTCTC 1 cut(s) 553
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 5 cut(s) 108, 219, 285, 458, 873
BfmI CTRYAG 3 cut(s) 228, 312, 981
BfuAI ACCTGC 1 cut(s) 235
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 2 cut(s) 1122, 1394
BlsI GCNGC 2 cut(s) 1123, 1395
BmcAI AGTACT 1 cut(s) 1256
Bme1390I CCNGG 2 cut(s) 946, 1107
Bme18I GGWCC 1 cut(s) 1289
BmgT120I GGNCC 5 cut(s) 27, 680, 1109, 1110, 1289
BmiI GGNNCC 3 cut(s) 682, 970, 1111
BmrFI CCNGG 2 cut(s) 946, 1107
BmsI GCATC 3 cut(s) 72, 775, 1405
BpiI GAAGAC 1 cut(s) 1084
BpmI CTGGAG 2 cut(s) 867, 1160
Bpu14I TTCGAA 1 cut(s) 1201
BpuEI CTTGAG 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 946
BsaAI YACGTR 1 cut(s) 1013
BsaJI CCNNGG 5 cut(s) 45, 153, 737, 1105, 1106
BsaWI WCCGGW 2 cut(s) 965, 1452
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 128, 452, 765
Bse1I ACTGG 4 cut(s) 847, 884, 1177, 1292
Bse3DI GCAATG 3 cut(s) 852, 1116, 1493
BseBI CCWGG 1 cut(s) 1107
BseDI CCNNGG 5 cut(s) 45, 153, 737, 1105, 1106
BseLI CCNNNNNNNGG 4 cut(s) 24, 128, 452, 765
BseMI GCAATG 3 cut(s) 852, 1116, 1493
BseMII CTCAG 3 cut(s) 566, 893, 987
BseNI ACTGG 4 cut(s) 847, 884, 1177, 1292
BseRI GAGGAG 3 cut(s) 158, 1265, 1496
BseSI GKGCMC 1 cut(s) 1113
BseXI GCAGC 2 cut(s) 1108, 1380
BseYI CCCAGC 1 cut(s) 1046
BshFI GGCC 7 cut(s) 29, 50, 152, 365, 399, 681, 1111
BsiHKAI GWGCWC 4 cut(s) 115, 355, 736, 775
BsiSI CCGG 3 cut(s) 945, 966, 1453
BslFI GGGAC 3 cut(s) 229, 1319, 1457
BslI CCNNNNNNNGG 4 cut(s) 24, 128, 452, 765
BsmAI GTCTC 1 cut(s) 553
BsmFI GGGAC 3 cut(s) 229, 1319, 1457
BsmI GAATGC 1 cut(s) 994
BsnI GGCC 7 cut(s) 29, 50, 152, 365, 399, 681, 1111
Bsp119I TTCGAA 1 cut(s) 1201
Bsp120I GGGCCC 1 cut(s) 1109
Bsp1286I GDGCHC 5 cut(s) 115, 355, 736, 775, 1113
Bsp143I GATC 5 cut(s) 159, 595, 624, 968, 1326
Bsp19I CCATGG 1 cut(s) 737
BspACI CCGC 4 cut(s) 337, 660, 1250, 1361
BspANI GGCC 7 cut(s) 29, 50, 152, 365, 399, 681, 1111
BspCNI CTCAG 3 cut(s) 565, 894, 986
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 3 cut(s) 682, 970, 1111
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 3 cut(s) 154, 963, 976
BspT104I TTCGAA 1 cut(s) 1201
BsrDI GCAATG 3 cut(s) 852, 1116, 1493
BsrI ACTGG 4 cut(s) 847, 884, 1177, 1292
BssECI CCNNGG 5 cut(s) 45, 153, 737, 1105, 1106
BssMI GATC 5 cut(s) 159, 595, 624, 968, 1326
BssT1I CCWWGG 2 cut(s) 153, 737
Bst2UI CCWGG 1 cut(s) 1107
Bst4CI ACNGT 7 cut(s) 268, 435, 655, 713, 961, 1283, 1414
BstBAI YACGTR 1 cut(s) 1013
BstBI TTCGAA 1 cut(s) 1201
BstC8I GCNNGC 3 cut(s) 339, 498, 1363
BstDEI CTNAG 5 cut(s) 385, 552, 902, 973, 1166
BstDSI CCRYGG 1 cut(s) 737
BstKTI GATC 5 cut(s) 162, 598, 627, 971, 1329
BstMAI GTCTC 1 cut(s) 553
BstMBI GATC 5 cut(s) 159, 595, 624, 968, 1326
BstMWI GCNNNNNNNGC 5 cut(s) 396, 794, 854, 1094, 1358
BstNI CCWGG 1 cut(s) 1107
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 944, 1105
BstSFI CTRYAG 3 cut(s) 228, 312, 981
BstSLI GKGCMC 1 cut(s) 1113
BstV1I GCAGC 2 cut(s) 1108, 1380
BstV2I GAAGAC 1 cut(s) 1084
BstX2I RGATCY 1 cut(s) 968
BstYI RGATCY 1 cut(s) 968
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 7 cut(s) 29, 50, 152, 365, 399, 681, 1111
BtgI CCRYGG 1 cut(s) 737
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 7 cut(s) 237, 301, 400, 431, 651, 840, 891
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 339, 498, 1363
CaiI CAGNNNCTG 1 cut(s) 980
CciI TCATGA 1 cut(s) 1053
Cfr13I GGNCC 5 cut(s) 27, 680, 1109, 1110, 1289
CseI GACGC 2 cut(s) 175, 804
Csp6I GTAC 3 cut(s) 705, 1255, 1267
CviAII CATG 4 cut(s) 587, 738, 1054, 1234
CviQI GTAC 3 cut(s) 705, 1255, 1267
DdeI CTNAG 5 cut(s) 385, 552, 902, 973, 1166
DpnI GATC 5 cut(s) 161, 597, 626, 970, 1328
DpnII GATC 5 cut(s) 159, 595, 624, 968, 1326
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 153, 737
Eco24I GRGCYC 2 cut(s) 355, 1113
Eco47I GGWCC 1 cut(s) 1289
Eco53kI GAGCTC 1 cut(s) 353
Eco57I CTGAAG 3 cut(s) 78, 359, 376
Eco72I CACGTG 1 cut(s) 1013
EcoICRI GAGCTC 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 680, 1109
EcoRII CCWGG 1 cut(s) 1105
EcoT14I CCWWGG 2 cut(s) 153, 737
EcoT38I GRGCYC 2 cut(s) 355, 1113
ErhI CCWWGG 2 cut(s) 153, 737
FaeI CATG 4 cut(s) 590, 741, 1057, 1237
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 3 cut(s) 229, 1319, 1457
FatI CATG 4 cut(s) 586, 737, 1053, 1233
FauI CCCGC 3 cut(s) 330, 653, 1354
FbaI TGATCA 1 cut(s) 1326
Fnu4HI GCNGC 2 cut(s) 1122, 1394
FriOI GRGCYC 2 cut(s) 355, 1113
Fsp4HI GCNGC 2 cut(s) 1122, 1394
FspBI CTAG 5 cut(s) 108, 219, 285, 458, 873
GluI GCNGC 2 cut(s) 1122, 1394
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 2 cut(s) 867, 1160
HaeIII GGCC 7 cut(s) 29, 50, 152, 365, 399, 681, 1111
HapII CCGG 3 cut(s) 945, 966, 1453
HgaI GACGC 2 cut(s) 175, 804
Hin1II CATG 4 cut(s) 590, 741, 1057, 1237
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HinfI GANTC 9 cut(s) 38, 291, 299, 471, 674, 836, 904, 934, 1469
HpaI GTTAAC 1 cut(s) 1008
HpaII CCGG 3 cut(s) 945, 966, 1453
HphI GGTGA 3 cut(s) 169, 803, 1478
Hpy166II GTNNAC 6 cut(s) 431, 840, 1008, 1269, 1297, 1433
Hpy188I TCNGA 4 cut(s) 378, 903, 939, 1348
Hpy188III TCNNGA 4 cut(s) 35, 163, 305, 1054
Hpy8I GTNNAC 6 cut(s) 431, 840, 1008, 1269, 1297, 1433
HpyAV CCTTC 6 cut(s) 142, 383, 769, 903, 1020, 1414
HpyCH4III ACNGT 7 cut(s) 268, 435, 655, 713, 961, 1283, 1414
HpyCH4IV ACGT 2 cut(s) 251, 1012
HpyCH4V TGCA 7 cut(s) 230, 416, 878, 1036, 1185, 1373, 1396
HpyF10VI GCNNNNNNNGC 5 cut(s) 396, 794, 854, 1094, 1358
HpyF3I CTNAG 5 cut(s) 385, 552, 902, 973, 1166
HpySE526I ACGT 2 cut(s) 251, 1012
Hsp92II CATG 4 cut(s) 590, 741, 1057, 1237
Ksp22I TGATCA 1 cut(s) 1326
KspAI GTTAAC 1 cut(s) 1008
Kzo9I GATC 5 cut(s) 159, 595, 624, 968, 1326
LmnI GCTCC 4 cut(s) 350, 739, 770, 1085
Lsp1109I GCAGC 2 cut(s) 1108, 1380
LweI GCATC 3 cut(s) 72, 775, 1405
MaeI CTAG 5 cut(s) 108, 219, 285, 458, 873
MaeII ACGT 2 cut(s) 251, 1012
MaeIII GTNAC 1 cut(s) 961
MalI GATC 5 cut(s) 161, 597, 626, 970, 1328
MboI GATC 5 cut(s) 159, 595, 624, 968, 1326
MboII GAAGA 4 cut(s) 47, 1084, 1222, 1320
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 1 cut(s) 968
MhlI GDGCHC 5 cut(s) 115, 355, 736, 775, 1113
MluCI AATT 5 cut(s) 20, 320, 342, 438, 1079
MlyI GAGTC 3 cut(s) 465, 845, 913
MseI TTAA 5 cut(s) 14, 324, 425, 1007, 1317
MslI CAYNNNNRTG 2 cut(s) 1401, 1528
MspI CCGG 3 cut(s) 945, 966, 1453
MspR9I CCNGG 2 cut(s) 946, 1107
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 994
MvaI CCWGG 1 cut(s) 1107
MwoI GCNNNNNNNGC 5 cut(s) 396, 794, 854, 1094, 1358
NciI CCSGG 1 cut(s) 946
NcoI CCATGG 1 cut(s) 737
NdeII GATC 5 cut(s) 159, 595, 624, 968, 1326
NlaIII CATG 4 cut(s) 590, 741, 1057, 1237
NlaIV GGNNCC 3 cut(s) 682, 970, 1111
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 961
NspI RCATGY 1 cut(s) 590
NspV TTCGAA 1 cut(s) 1201
PagI TCATGA 1 cut(s) 1053
PasI CCCWGGG 1 cut(s) 1106
PciI ACATGT 1 cut(s) 586
PctI GAATGC 1 cut(s) 994
PfeI GAWTC 6 cut(s) 38, 291, 299, 674, 934, 1469
PflFI GACNNNGTC 1 cut(s) 814
PflMI CCANNNNNTGG 1 cut(s) 24
PkrI GCNGC 2 cut(s) 1123, 1395
PleI GAGTC 3 cut(s) 465, 844, 912
PmaCI CACGTG 1 cut(s) 1013
PmlI CACGTG 1 cut(s) 1013
PpsI GAGTC 3 cut(s) 465, 844, 912
Ppu21I YACGTR 1 cut(s) 1013
PscI ACATGT 1 cut(s) 586
Psp124BI GAGCTC 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 1105
PspCI CACGTG 1 cut(s) 1013
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 1 cut(s) 1105
PspN4I GGNNCC 3 cut(s) 682, 970, 1111
PspOMI GGGCCC 1 cut(s) 1109
PspPI GGNCC 5 cut(s) 27, 680, 1109, 1110, 1289
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 980
PsuI RGATCY 1 cut(s) 968
PsyI GACNNNGTC 1 cut(s) 814
RsaI GTAC 3 cut(s) 706, 1256, 1268
RsaNI GTAC 3 cut(s) 705, 1255, 1267
RseI CAYNNNNRTG 2 cut(s) 1401, 1528
SacI GAGCTC 1 cut(s) 355
SaqAI TTAA 5 cut(s) 14, 324, 425, 1007, 1317
SatI GCNGC 2 cut(s) 1122, 1394
Sau3AI GATC 5 cut(s) 159, 595, 624, 968, 1326
Sau96I GGNCC 5 cut(s) 27, 680, 1109, 1110, 1289
ScaI AGTACT 1 cut(s) 1256
SchI GAGTC 3 cut(s) 465, 845, 913
ScrFI CCNGG 2 cut(s) 946, 1107
SduI GDGCHC 5 cut(s) 115, 355, 736, 775, 1113
SfaNI GCATC 3 cut(s) 72, 775, 1405
SfcI CTRYAG 3 cut(s) 228, 312, 981
SfuI TTCGAA 1 cut(s) 1201
SinI GGWCC 1 cut(s) 1289
SmiMI CAYNNNNRTG 2 cut(s) 1401, 1528
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 5 cut(s) 20, 320, 342, 438, 1079
SsiI CCGC 4 cut(s) 337, 660, 1250, 1361
SspMI CTAG 5 cut(s) 108, 219, 285, 458, 873
SstI GAGCTC 1 cut(s) 355
StyD4I CCNGG 2 cut(s) 944, 1105
StyI CCWWGG 2 cut(s) 153, 737
TaaI ACNGT 7 cut(s) 268, 435, 655, 713, 961, 1283, 1414
TaiI ACGT 2 cut(s) 254, 1015
TaqI TCGA 4 cut(s) 289, 1024, 1201, 1399
TasI AATT 5 cut(s) 20, 320, 342, 438, 1079
TatI WGTACW 2 cut(s) 1254, 1266
TfiI GAWTC 6 cut(s) 38, 291, 299, 674, 934, 1469
Tru1I TTAA 5 cut(s) 14, 324, 425, 1007, 1317
Tru9I TTAA 5 cut(s) 14, 324, 425, 1007, 1317
TscAI CASTG 7 cut(s) 237, 301, 400, 438, 658, 847, 891
TseFI GTSAC 1 cut(s) 961
TseI GCWGC 2 cut(s) 1121, 1393
Tsp45I GTSAC 1 cut(s) 961
TspDTI ATGAA 5 cut(s) 841, 1042, 1201, 1222, 1482
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 7 cut(s) 237, 301, 400, 438, 658, 847, 891
Tth111I GACNNNGTC 1 cut(s) 814
Van91I CCANNNNNTGG 1 cut(s) 24
VpaK11BI GGWCC 1 cut(s) 1289
XapI RAATTY 2 cut(s) 320, 1079
XceI RCATGY 1 cut(s) 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 5 cut(s) 108, 219, 285, 458, 873
ZrmI AGTACT 1 cut(s) 1256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.