Rmu_sc0000850.1_g000014
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000850.1
OG0022974
Physical Location & Seq
Forward (+)
50416 .. 52694
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000850.1_g000014.1.cds

Sequence Viewer

Length: 1770 bp
atgcttgaactcaactctcaactgaagcagctagtcaaaatttgtaacgttaaagacgcccaaaagatgttcgataaaaggcctaagagagatgcggtttcatggaccgagtacttggcgaaatcgggtattttgaactctgtcattgtgggaagtactcttcttgtcttgtatatgaaggttgttgggattgagaagggttgtatagttttcgataaaatgccgctaagaaatgtggtgtctgggactaccattatcactggtgaaattgtaaccaaaagtgttgaagtgtcaaaacgagctggccaggatgtcacagcaaatcaccgaagtggtgcaactgaggtacccatgcggtcaaatgaatctcctctgcttatgttgctgactgcaattatacactcccagataatggaatctgtgatttcaagccatgtactacaggatactatttttgagaagaagcacaagcagacactaaataagttggaaggaattctaactgatccattgaaagcactggaagcactggaattgatgttccaaggagaggcaaccaaaattttaaaggaaatgttgctgtgttataagccaaatgcagagcggacattgaaatatggtcaggtatttttgcaatgttctgaccatgctatcttcaatggcagcactacttcaaccgcaggccaagacaattttactattgagtgcaatgtaattattgctaacaacgcatgtttacatttgggagatgagcgttttcttatggatggagtgagtgaggtttctgatcattatgcttggctggactttctaacaagcgaagttccagggacaaaggtaataaaatttacaaaggaaagtcttgctttccagagatattgttcaatggatgcatttggttccaagaaggaagtcattgctttcgtaggtgagatctataagcattgttctatggttgtggaagcttctatagtactgtggtatttttctcttctacgttctcctttggatagtgatgatttcaggtctggaatgttcaagttgattcatcacggggttctccttgttcatgtacaagtcaatccagaattattttctttgaggtaccttaaaaactatctcatgctttactctatatcaaacaaaaacgtacaagaaatcaagatcagtccaggtctcatgttgctgattttcaataaagctagtacccatgttctgctgaagattatgtccatagaagatggcacagttctcaaagcttttaacaacattcttcatcaaaataaggtgtctttcatagaacaattcaatgaaaagcttcttgttaagcaggaaaatgaaaatctccagattcttgatgttcgaaatgtggagctgctggaagttaacagagctgagttcctgacatcatcatcatttttcattctgtatgagaaccaactattcctgacattcaaaaatagaactaagcttttcttggttgattgggcttttgcaaatgtaccaatatcttggaaggatatgaaaaccaagttgtttgctctttatatggttactaaggtactccaacaggaaggtcaaatctgtgatttctcggccattatgcagttggttgtgattttgtccactaggtctttgaatgaactgaagggaatcatgtgcattctgcatagttctcttgcagacattgatgactcctattctaagtggattttgactcatgtgaaacttgaggacaagttctctgtgaagcagggtggattgttatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

589

Amino Acids

66.78

Weight (kDa)

7.58

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022974)

Species Orthologous Gene IDs
rosa_laevigata RLG00000029498
rosa_multiflora Rmu_sc0000850.1_g000014 Rmu_sc0002571.1_g000010 Rmu_sc0008835.1_g000008

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
Acc65I GGTACC 2 cut(s) 346, 1104
AccB1I GGYRCC 2 cut(s) 346, 1104
AccB7I CCANNNNNTGG 1 cut(s) 412
AccBSI CCGCTC 1 cut(s) 604
AciI CCGC 5 cut(s) 95, 224, 355, 604, 678
AclI AACGTT 1 cut(s) 48
AclWI GGATC 1 cut(s) 500
AcoI YGGCCR 2 cut(s) 304, 1596
AcsI RAATTY 4 cut(s) 39, 495, 561, 845
AcuI CTGAAG 3 cut(s) 44, 1241, 1667
AcyI GRCGYC 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 412, 550
AjnI CCWGG 3 cut(s) 306, 826, 1171
AleI CACNNNNGTG 1 cut(s) 330
AloI GAACNNNNNNTCC 2 cut(s) 524, 556
AluBI AGCT 9 cut(s) 31, 302, 965, 1202, 1259, 1318, 1375, 1394, 1471
AluI AGCT 9 cut(s) 31, 302, 965, 1202, 1259, 1318, 1375, 1394, 1471
Alw26I GTCTC 1 cut(s) 1181
AlwI GGATC 1 cut(s) 500
AoxI GGCC 4 cut(s) 80, 304, 682, 1596
ApeKI GCWGC 3 cut(s) 28, 663, 1375
ApoI RAATTY 4 cut(s) 39, 495, 561, 845
Asp700I GAANNNNTTC 2 cut(s) 495, 1317
Asp718I GGTACC 2 cut(s) 346, 1104
AspS9I GGNCC 1 cut(s) 105
AsuHPI GGTGA 3 cut(s) 275, 317, 941
AsuII TTCGAA 1 cut(s) 1363
AvaII GGWCC 1 cut(s) 105
BaeI ACNNNNGTAYC 2 cut(s) 330, 363
BalI TGGCCA 1 cut(s) 306
BanI GGYRCC 2 cut(s) 346, 1104
BbvI GCAGC 3 cut(s) 40, 675, 1362
BccI CCATC 2 cut(s) 761, 1235
BciT130I CCWGG 3 cut(s) 308, 828, 1173
BciVI GTATCC 1 cut(s) 439
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 1 cut(s) 1181
BfaI CTAG 3 cut(s) 32, 1203, 1629
BfmI CTRYAG 2 cut(s) 440, 969
BfuI GTATCC 1 cut(s) 439
BglII AGATCT 1 cut(s) 933
BisI GCNGC 4 cut(s) 29, 224, 664, 1376
BlsI GCNGC 4 cut(s) 30, 225, 665, 1377
BmcAI AGTACT 3 cut(s) 113, 157, 975
Bme1390I CCNGG 3 cut(s) 308, 828, 1173
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 3 cut(s) 348, 901, 1106
BmrFI CCNGG 3 cut(s) 308, 828, 1173
BmsI GCATC 2 cut(s) 82, 880
BpmI CTGGAG 1 cut(s) 1331
Bpu14I TTCGAA 1 cut(s) 1363
BpuEI CTTGAG 1 cut(s) 1751
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 2 cut(s) 544, 827
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 550
Bse1I ACTGG 3 cut(s) 265, 525, 534
Bse3DI GCAATG 3 cut(s) 641, 715, 915
BseBI CCWGG 3 cut(s) 308, 828, 1173
BseDI CCNNGG 2 cut(s) 544, 827
BseGI GGATG 3 cut(s) 316, 772, 895
BseLI CCNNNNNNNGG 2 cut(s) 412, 550
BseMI GCAATG 3 cut(s) 641, 715, 915
BseMII CTCAG 2 cut(s) 333, 1386
BseNI ACTGG 3 cut(s) 265, 525, 534
BseRI GAGGAG 1 cut(s) 360
BseXI GCAGC 3 cut(s) 40, 675, 1362
BshFI GGCC 4 cut(s) 82, 306, 684, 1598
BshNI GGYRCC 2 cut(s) 346, 1104
BslFI GGGAC 2 cut(s) 259, 844
BslI CCNNNNNNNGG 2 cut(s) 412, 550
BsmAI GTCTC 1 cut(s) 1181
BsmFI GGGAC 2 cut(s) 259, 844
BsmI GAATGC 1 cut(s) 1662
BsnI GGCC 4 cut(s) 82, 306, 684, 1598
Bso31I GGTCTC 1 cut(s) 1181
Bsp119I TTCGAA 1 cut(s) 1363
Bsp1407I TGTACA 1 cut(s) 1072
Bsp143I GATC 4 cut(s) 505, 787, 933, 1164
BspACI CCGC 5 cut(s) 95, 224, 355, 604, 678
BspANI GGCC 4 cut(s) 82, 306, 684, 1598
BspCNI CTCAG 2 cut(s) 334, 1387
BspLI GGNNCC 3 cut(s) 348, 901, 1106
BspPI GGATC 1 cut(s) 500
BspT104I TTCGAA 1 cut(s) 1363
BspT107I GGYRCC 2 cut(s) 346, 1104
BspTNI GGTCTC 1 cut(s) 1181
BsrBI CCGCTC 1 cut(s) 604
BsrDI GCAATG 3 cut(s) 641, 715, 915
BsrGI TGTACA 1 cut(s) 1072
BsrI ACTGG 3 cut(s) 265, 525, 534
BssECI CCNNGG 2 cut(s) 544, 827
BssMI GATC 4 cut(s) 505, 787, 933, 1164
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 544
Bst2UI CCWGG 3 cut(s) 308, 828, 1173
Bst4CI ACNGT 2 cut(s) 978, 1249
Bst6I CTCTTC 2 cut(s) 165, 996
BstACI GRCGYC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 1072
BstBI TTCGAA 1 cut(s) 1363
BstC8I GCNNGC 2 cut(s) 304, 682
BstDEI CTNAG 7 cut(s) 84, 227, 342, 1395, 1467, 1557, 1704
BstF5I GGATG 3 cut(s) 316, 772, 895
BstKTI GATC 4 cut(s) 508, 790, 936, 1167
BstMAI GTCTC 1 cut(s) 1181
BstMBI GATC 4 cut(s) 505, 787, 933, 1164
BstMWI GCNNNNNNNGC 3 cut(s) 382, 524, 728
BstNI CCWGG 3 cut(s) 308, 828, 1173
BstNSI RCATGY 1 cut(s) 735
BstSCI CCNGG 3 cut(s) 306, 826, 1171
BstSFI CTRYAG 2 cut(s) 440, 969
BstV1I GCAGC 3 cut(s) 40, 675, 1362
BstX2I RGATCY 1 cut(s) 933
BstXI CCANNNNNNTGG 1 cut(s) 1512
BstYI RGATCY 1 cut(s) 933
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 4 cut(s) 82, 306, 684, 1598
BtsCI GGATG 3 cut(s) 316, 772, 895
BtsIMutI CAGTG 3 cut(s) 258, 518, 527
Cac8I GCNNGC 2 cut(s) 304, 682
Cfr13I GGNCC 1 cut(s) 105
CseI GACGC 1 cut(s) 65
DdeI CTNAG 7 cut(s) 84, 227, 342, 1395, 1467, 1557, 1704
DpnI GATC 4 cut(s) 507, 789, 935, 1166
DpnII GATC 4 cut(s) 505, 787, 933, 1164
DraI TTTAAA 1 cut(s) 567
EaeI YGGCCR 2 cut(s) 304, 1596
Eam1104I CTCTTC 2 cut(s) 165, 996
EarI CTCTTC 2 cut(s) 165, 996
Eco130I CCWWGG 1 cut(s) 544
Eco147I AGGCCT 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 1181
Eco47I GGWCC 1 cut(s) 105
Eco57I CTGAAG 3 cut(s) 44, 1241, 1667
EcoRI GAATTC 1 cut(s) 495
EcoRII CCWGG 3 cut(s) 306, 826, 1171
EcoT14I CCWWGG 1 cut(s) 544
EcoT22I ATGCAT 1 cut(s) 895
ErhI CCWWGG 1 cut(s) 544
FalI AAGNNNNNCTT 4 cut(s) 850, 882, 1460, 1492
FaqI GGGAC 2 cut(s) 259, 844
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 4 cut(s) 29, 224, 664, 1376
FokI GGATG 3 cut(s) 323, 779, 902
Fsp4HI GCNGC 4 cut(s) 29, 224, 664, 1376
FspBI CTAG 3 cut(s) 32, 1203, 1629
GluI GCNGC 4 cut(s) 29, 224, 664, 1376
GsuI CTGGAG 1 cut(s) 1331
HaeIII GGCC 4 cut(s) 82, 306, 684, 1598
HgaI GACGC 1 cut(s) 65
Hin1I GRCGYC 1 cut(s) 57
HincII GTYRAC 1 cut(s) 1387
HindII GTYRAC 1 cut(s) 1387
HindIII AAGCTT 4 cut(s) 963, 1257, 1316, 1469
HinfI GANTC 7 cut(s) 365, 416, 1045, 1351, 1653, 1694, 1717
HpaI GTTAAC 1 cut(s) 1387
HphI GGTGA 3 cut(s) 275, 317, 941
Hpy166II GTNNAC 3 cut(s) 737, 1387, 1626
Hpy188I TCNGA 2 cut(s) 643, 787
Hpy188III TCNNGA 8 cut(s) 871, 1029, 1085, 1162, 1348, 1355, 1402, 1447
Hpy8I GTNNAC 3 cut(s) 737, 1387, 1626
HpyAV CCTTC 7 cut(s) 172, 190, 485, 901, 1510, 1568, 1642
HpyCH4III ACNGT 2 cut(s) 978, 1249
HpyCH4IV ACGT 3 cut(s) 48, 997, 1149
HpyF10VI GCNNNNNNNGC 3 cut(s) 382, 524, 728
HpyF3I CTNAG 7 cut(s) 84, 227, 342, 1395, 1467, 1557, 1704
HpySE526I ACGT 3 cut(s) 48, 997, 1149
Hsp92I GRCGYC 1 cut(s) 57
KpnI GGTACC 2 cut(s) 350, 1108
Ksp22I TGATCA 1 cut(s) 787
KspAI GTTAAC 1 cut(s) 1387
Kzo9I GATC 4 cut(s) 505, 787, 933, 1164
LmnI GCTCC 1 cut(s) 1372
Lsp1109I GCAGC 3 cut(s) 40, 675, 1362
LweI GCATC 2 cut(s) 82, 880
MaeI CTAG 3 cut(s) 32, 1203, 1629
MaeII ACGT 3 cut(s) 48, 997, 1149
MaeIII GTNAC 4 cut(s) 44, 271, 313, 1552
MalI GATC 4 cut(s) 507, 789, 935, 1166
MbiI CCGCTC 1 cut(s) 604
MboI GATC 4 cut(s) 505, 787, 933, 1164
MboII GAAGA 7 cut(s) 152, 472, 646, 983, 1234, 1250, 1265
MflI RGATCY 1 cut(s) 933
MlsI TGGCCA 1 cut(s) 306
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 2 cut(s) 1688, 1711
MmeI TCCRAC 2 cut(s) 468, 1591
MnlI CCTC 6 cut(s) 337, 381, 544, 772, 1095, 1726
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 1 cut(s) 895
MroXI GAANNNNTTC 2 cut(s) 495, 1317
MscI TGGCCA 1 cut(s) 306
MseI TTAA 6 cut(s) 51, 566, 1110, 1263, 1326, 1386
MslI CAYNNNNRTG 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 306
MspR9I CCNGG 3 cut(s) 308, 828, 1173
Mva1269I GAATGC 1 cut(s) 1662
MvaI CCWGG 3 cut(s) 308, 828, 1173
MwoI GCNNNNNNNGC 3 cut(s) 382, 524, 728
NdeII GATC 4 cut(s) 505, 787, 933, 1164
NlaIV GGNNCC 3 cut(s) 348, 901, 1106
NmeAIII GCCGAG 1 cut(s) 1574
NmuCI GTSAC 1 cut(s) 313
NsiI ATGCAT 1 cut(s) 895
NspI RCATGY 1 cut(s) 735
NspV TTCGAA 1 cut(s) 1363
OliI CACNNNNGTG 1 cut(s) 330
PceI AGGCCT 1 cut(s) 82
PctI GAATGC 1 cut(s) 1662
PdmI GAANNNNTTC 2 cut(s) 495, 1317
PfeI GAWTC 5 cut(s) 365, 416, 1045, 1351, 1653
PflMI CCANNNNNTGG 1 cut(s) 412
PkrI GCNGC 4 cut(s) 30, 225, 665, 1377
PleI GAGTC 2 cut(s) 1688, 1711
PpsI GAGTC 2 cut(s) 1688, 1711
PsiI TTATAA 1 cut(s) 588
Psp1406I AACGTT 1 cut(s) 48
Psp6I CCWGG 3 cut(s) 306, 826, 1171
PspGI CCWGG 3 cut(s) 306, 826, 1171
PspN4I GGNNCC 3 cut(s) 348, 901, 1106
PspPI GGNCC 1 cut(s) 105
PsuI RGATCY 1 cut(s) 933
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 6 cut(s) 51, 566, 1110, 1263, 1326, 1386
SatI GCNGC 4 cut(s) 29, 224, 664, 1376
Sau3AI GATC 4 cut(s) 505, 787, 933, 1164
Sau96I GGNCC 1 cut(s) 105
ScaI AGTACT 3 cut(s) 113, 157, 975
SchI GAGTC 2 cut(s) 1688, 1711
ScrFI CCNGG 3 cut(s) 308, 828, 1173
SfaNI GCATC 2 cut(s) 82, 880
SfcI CTRYAG 2 cut(s) 440, 969
SfuI TTCGAA 1 cut(s) 1363
SinI GGWCC 1 cut(s) 105
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 1730
SmoI CTYRAG 1 cut(s) 1730
SseBI AGGCCT 1 cut(s) 82
SsiI CCGC 5 cut(s) 95, 224, 355, 604, 678
SspMI CTAG 3 cut(s) 32, 1203, 1629
StuI AGGCCT 1 cut(s) 82
StyD4I CCNGG 3 cut(s) 306, 826, 1171
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 2 cut(s) 978, 1249
TaiI ACGT 3 cut(s) 51, 1000, 1152
TaqI TCGA 3 cut(s) 72, 213, 1363
TaqII GACCGA 1 cut(s) 122
TatI WGTACW 5 cut(s) 111, 155, 436, 973, 1072
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 5 cut(s) 365, 416, 1045, 1351, 1653
Tru1I TTAA 6 cut(s) 51, 566, 1110, 1263, 1326, 1386
Tru9I TTAA 6 cut(s) 51, 566, 1110, 1263, 1326, 1386
TscAI CASTG 3 cut(s) 265, 525, 534
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 3 cut(s) 28, 663, 1375
Tsp45I GTSAC 1 cut(s) 313
TspRI CASTG 3 cut(s) 265, 525, 534
Van91I CCANNNNNTGG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 105
XapI RAATTY 4 cut(s) 39, 495, 561, 845
XceI RCATGY 1 cut(s) 735
XcmI CCANNNNNNNNNTGG 1 cut(s) 1606
XmnI GAANNNNTTC 2 cut(s) 495, 1317
XspI CTAG 3 cut(s) 32, 1203, 1629
ZrmI AGTACT 3 cut(s) 113, 157, 975
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.