Rmu_sc0000903.1_g000032

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000903.1
OG0021052
Physical Location & Seq
Reverse (-)
126949 .. 127935
987 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 750 bp
atgcagtatcgatttcagttcctttactggaaatggcaacaccgacctgaggccagccctagtttctctatgcttcctgggaaaacgattttgttaaaatggcatcgagtcttttcaatgattttcaaagtgttgaaagaaaaaaccctaagcgcgtcgtctacacgtactaaaaccccaaacattgccggcctcctggctgcatctctttcaccgcaacaccatctcaccatggcttccattgaggatgttactacatcgctggccttggtaaggaacgagatcattgatctctctcggagaacgggggtgtttgggtcctggataaaggctttgatcaaaattcaagaatacaaaacaataactttgtcctggctttcaatctacttcgagaccgcaaccaaagtgcttcgaggaggaccttggaggtttcaaccaggccccggtgtactacaggagtatgacggcatggttctgctgcatacagtggagatgaactccttcttctactgggtgcaaatcaccaacatcccttctgcttttgaggatctagataccatcagagacattgcctctgttgctggtgagttccttgaattggatgaaaagctgtttgagtatatgggaagagggggccgggaccagtgtcccttaagcattggacctgtaaaggtagtggagaagaggcttgatattgtaagctcatctttgggtttccctgcaagtaagtttatggatggagctttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_3 zf-CCHC_4

Protein Analysis

249

Amino Acids

28.34

Weight (kDa)

9.1

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021052)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g33131
rosa_multiflora Rmu_sc0000903.1_g000032 Rmu_sc0001211.1_g000125 Rmu_sc0003846.1_g000007 Rmu_sc0007066.1_g000010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AccII CGCG 1 cut(s) 155
AciI CCGC 2 cut(s) 215, 396
AclWI GGATC 1 cut(s) 555
AcsI RAATTY 1 cut(s) 342
AfaI GTAC 2 cut(s) 169, 450
AfiI CCNNNNNNNGG 4 cut(s) 49, 273, 443, 598
AflII CTTAAG 1 cut(s) 652
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 7 cut(s) 117, 127, 136, 347, 381, 434, 596
AjnI CCWGG 5 cut(s) 76, 195, 320, 371, 436
AluBI AGCT 3 cut(s) 610, 702, 743
AluI AGCT 3 cut(s) 610, 702, 743
Alw26I GTCTC 2 cut(s) 386, 558
AlwI GGATC 1 cut(s) 555
AoxI GGCC 5 cut(s) 51, 190, 264, 439, 634
ApeKI GCWGC 2 cut(s) 200, 478
ApoI RAATTY 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 500
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 6 cut(s) 318, 419, 440, 634, 640, 662
AsuC2I CCSGG 2 cut(s) 444, 638
AsuHPI GGTGA 4 cut(s) 204, 220, 514, 596
AvaII GGWCC 4 cut(s) 318, 419, 640, 662
AxyI CCTNAGG 1 cut(s) 48
BbvI GCAGC 2 cut(s) 187, 465
BccI CCATC 3 cut(s) 231, 566, 731
BceAI ACGGC 1 cut(s) 481
BciT130I CCWGG 5 cut(s) 78, 197, 322, 373, 438
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 2 cut(s) 444, 638
BcoDI GTCTC 2 cut(s) 386, 558
BfaI CTAG 2 cut(s) 60, 551
BfmI CTRYAG 1 cut(s) 452
BfrI CTTAAG 1 cut(s) 652
BisI GCNGC 2 cut(s) 201, 479
BlsI GCNGC 2 cut(s) 202, 480
Bme1390I CCNGG 7 cut(s) 78, 197, 322, 373, 438, 444, 638
Bme18I GGWCC 4 cut(s) 318, 419, 640, 662
BmgT120I GGNCC 6 cut(s) 318, 419, 440, 634, 640, 662
BmiI GGNNCC 4 cut(s) 319, 442, 635, 641
BmrFI CCNGG 7 cut(s) 78, 197, 322, 373, 438, 444, 638
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 2 cut(s) 112, 212
BmuI ACTGGG 1 cut(s) 520
BoxI GACNNNNGTC 1 cut(s) 645
BplI GAGNNNNNCTC 2 cut(s) 482, 514
Bpu10I CCTNAGC 1 cut(s) 149
BpuMI CCSGG 2 cut(s) 444, 638
Bsa29I ATCGAT 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 167
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 5 cut(s) 77, 231, 267, 422, 442
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 273, 443, 598
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 3 cut(s) 32, 515, 643
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 2 cut(s) 183, 567
BseBI CCWGG 5 cut(s) 78, 197, 322, 373, 438
BseCI ATCGAT 1 cut(s) 10
BseDI CCNNGG 5 cut(s) 77, 231, 267, 422, 442
BseGI GGATG 4 cut(s) 253, 528, 607, 742
BseLI CCNNNNNNNGG 4 cut(s) 49, 273, 443, 598
BseMI GCAATG 2 cut(s) 183, 567
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 3 cut(s) 32, 515, 643
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 2 cut(s) 187, 465
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 5 cut(s) 53, 192, 266, 441, 636
BshVI ATCGAT 1 cut(s) 10
BsiSI CCGG 3 cut(s) 189, 444, 637
BslFI GGGAC 2 cut(s) 633, 653
BslI CCNNNNNNNGG 4 cut(s) 49, 273, 443, 598
BsmAI GTCTC 2 cut(s) 386, 558
BsmFI GGGAC 2 cut(s) 633, 653
BsnI GGCC 5 cut(s) 53, 192, 266, 441, 636
Bso31I GGTCTC 1 cut(s) 386
Bsp143I GATC 4 cut(s) 282, 289, 336, 547
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 2 cut(s) 215, 396
BspANI GGCC 5 cut(s) 53, 192, 266, 441, 636
BspCNI CTCAG 1 cut(s) 40
BspDI ATCGAT 1 cut(s) 10
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 4 cut(s) 319, 442, 635, 641
BspPI GGATC 1 cut(s) 555
BspTI CTTAAG 1 cut(s) 652
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 183, 567
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 3 cut(s) 32, 515, 643
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 5 cut(s) 77, 231, 267, 422, 442
BssMI GATC 4 cut(s) 282, 289, 336, 547
BssT1I CCWWGG 3 cut(s) 231, 267, 422
Bst2UI CCWGG 5 cut(s) 78, 197, 322, 373, 438
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 2 cut(s) 622, 677
BstAFI CTTAAG 1 cut(s) 652
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 55, 190, 264
BstDEI CTNAG 2 cut(s) 48, 149
BstDSI CCRYGG 1 cut(s) 231
BstENI CCTNNNNNAGG 1 cut(s) 271
BstF5I GGATG 4 cut(s) 253, 528, 607, 742
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 4 cut(s) 285, 292, 339, 550
BstMAI GTCTC 2 cut(s) 386, 558
BstMBI GATC 4 cut(s) 282, 289, 336, 547
BstMWI GCNNNNNNNGC 1 cut(s) 578
BstNI CCWGG 5 cut(s) 78, 197, 322, 373, 438
BstPAI GACNNNNGTC 1 cut(s) 645
BstSCI CCNGG 7 cut(s) 76, 195, 320, 371, 436, 442, 636
BstSFI CTRYAG 1 cut(s) 452
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 187, 465
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 10
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 5 cut(s) 53, 192, 266, 441, 636
BsuTUI ATCGAT 1 cut(s) 10
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 4 cut(s) 253, 528, 607, 742
BtsIMutI CAGTG 2 cut(s) 492, 650
Cac8I GCNNGC 3 cut(s) 55, 190, 264
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 6 cut(s) 318, 419, 440, 634, 640, 662
ClaI ATCGAT 1 cut(s) 10
CseI GACGC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 168, 449
CviAII CATG 2 cut(s) 232, 469
CviQI GTAC 2 cut(s) 168, 449
DdeI CTNAG 2 cut(s) 48, 149
DpnI GATC 4 cut(s) 284, 291, 338, 549
DpnII GATC 4 cut(s) 282, 289, 336, 547
Eam1104I CTCTTC 2 cut(s) 622, 677
EarI CTCTTC 2 cut(s) 622, 677
Eco130I CCWWGG 3 cut(s) 231, 267, 422
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 4 cut(s) 318, 419, 640, 662
Eco81I CCTNAGG 1 cut(s) 48
EcoNI CCTNNNNNAGG 1 cut(s) 271
EcoO109I RGGNCCY 3 cut(s) 318, 419, 440
EcoRII CCWGG 5 cut(s) 76, 195, 320, 371, 436
EcoT14I CCWWGG 3 cut(s) 231, 267, 422
ErhI CCWWGG 3 cut(s) 231, 267, 422
FaeI CATG 2 cut(s) 235, 472
FaiI YATR 8 cut(s) 71, 233, 462, 470, 483, 621, 623, 734
FalI AAGNNNNNCTT 2 cut(s) 691, 723
FaqI GGGAC 2 cut(s) 633, 653
FatI CATG 2 cut(s) 231, 468
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 201, 479
FokI GGATG 3 cut(s) 260, 515, 614
Fsp4HI GCNGC 2 cut(s) 201, 479
FspBI CTAG 2 cut(s) 60, 551
GlaI GCGC 1 cut(s) 154
GluI GCNGC 2 cut(s) 201, 479
HaeIII GGCC 5 cut(s) 53, 192, 266, 441, 636
HapII CCGG 3 cut(s) 189, 444, 637
HgaI GACGC 1 cut(s) 144
HhaI GCGC 1 cut(s) 155
Hin1II CATG 2 cut(s) 235, 472
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 1 cut(s) 108
HpaII CCGG 3 cut(s) 189, 444, 637
HphI GGTGA 4 cut(s) 204, 220, 514, 596
Hpy166II GTNNAC 2 cut(s) 162, 449
Hpy188I TCNGA 3 cut(s) 300, 563, 749
Hpy188III TCNNGA 3 cut(s) 347, 391, 551
Hpy8I GTNNAC 2 cut(s) 162, 449
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 511, 543
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 5 cut(s) 4, 203, 481, 517, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 578
HpyF3I CTNAG 2 cut(s) 48, 149
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 2 cut(s) 235, 472
HspAI GCGC 1 cut(s) 153
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 4 cut(s) 282, 289, 336, 547
LmnI GCTCC 1 cut(s) 740
Lsp1109I GCAGC 2 cut(s) 187, 465
LweI GCATC 2 cut(s) 112, 212
MaeI CTAG 2 cut(s) 60, 551
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 1 cut(s) 250
MalI GATC 4 cut(s) 284, 291, 338, 549
MboI GATC 4 cut(s) 282, 289, 336, 547
MboII GAAGA 3 cut(s) 496, 639, 694
MflI RGATCY 1 cut(s) 547
MluCI AATT 2 cut(s) 342, 596
MlyI GAGTC 1 cut(s) 117
MroNI GCCGGC 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 500
MseI TTAA 2 cut(s) 95, 653
MspCI CTTAAG 1 cut(s) 652
MspI CCGG 3 cut(s) 189, 444, 637
MspR9I CCNGG 7 cut(s) 78, 197, 322, 373, 438, 444, 638
MvaI CCWGG 5 cut(s) 78, 197, 322, 373, 438
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 578
NaeI GCCGGC 1 cut(s) 190
NciI CCSGG 2 cut(s) 444, 638
NcoI CCATGG 1 cut(s) 231
NdeII GATC 4 cut(s) 282, 289, 336, 547
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 2 cut(s) 235, 472
NlaIV GGNNCC 4 cut(s) 319, 442, 635, 641
PdiI GCCGGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 500
PfoI TCCNGGA 1 cut(s) 320
PkrI GCNGC 2 cut(s) 202, 480
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Ppu21I YACGTR 1 cut(s) 167
PpuMI RGGWCCY 2 cut(s) 318, 419
PshAI GACNNNNGTC 1 cut(s) 645
Psp5II RGGWCCY 2 cut(s) 318, 419
Psp6I CCWGG 5 cut(s) 76, 195, 320, 371, 436
PspGI CCWGG 5 cut(s) 76, 195, 320, 371, 436
PspN4I GGNNCC 4 cut(s) 319, 442, 635, 641
PspPI GGNCC 6 cut(s) 318, 419, 440, 634, 640, 662
PspPPI RGGWCCY 2 cut(s) 318, 419
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 2 cut(s) 169, 450
RsaNI GTAC 2 cut(s) 168, 449
SaqAI TTAA 2 cut(s) 95, 653
SatI GCNGC 2 cut(s) 201, 479
Sau3AI GATC 4 cut(s) 282, 289, 336, 547
Sau96I GGNCC 6 cut(s) 318, 419, 440, 634, 640, 662
SchI GAGTC 1 cut(s) 117
ScrFI CCNGG 7 cut(s) 78, 197, 322, 373, 438, 444, 638
SetI ASST 9 cut(s) 49, 169, 424, 431, 612, 667, 675, 704, 745
SfaNI GCATC 2 cut(s) 112, 212
SfcI CTRYAG 1 cut(s) 452
SinI GGWCC 4 cut(s) 318, 419, 640, 662
SmlI CTYRAG 1 cut(s) 652
SmoI CTYRAG 1 cut(s) 652
Sse9I AATT 2 cut(s) 342, 596
SsiI CCGC 2 cut(s) 215, 396
SspMI CTAG 2 cut(s) 60, 551
StyD4I CCNGG 7 cut(s) 76, 195, 320, 371, 436, 442, 636
StyI CCWWGG 3 cut(s) 231, 267, 422
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 1 cut(s) 169
TaqI TCGA 4 cut(s) 10, 106, 390, 412
TasI AATT 2 cut(s) 342, 596
TatI WGTACW 1 cut(s) 448
Tru1I TTAA 2 cut(s) 95, 653
Tru9I TTAA 2 cut(s) 95, 653
TscAI CASTG 2 cut(s) 492, 650
TseI GCWGC 2 cut(s) 200, 478
TspDTI ATGAA 2 cut(s) 509, 618
TspRI CASTG 2 cut(s) 492, 650
Vha464I CTTAAG 1 cut(s) 652
VpaK11BI GGWCC 4 cut(s) 318, 419, 640, 662
XagI CCTNNNNNAGG 1 cut(s) 271
XapI RAATTY 1 cut(s) 342
XbaI TCTAGA 1 cut(s) 550
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 2 cut(s) 60, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.