Rmu_sc0001147.1_g000006

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001147.1
OG0020066
Physical Location & Seq
Forward (+)
41861 .. 47364
5504 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 672 bp
atgtttagctggttgctccttaggggaagactaaagaccagagatagggtctcaaggtttagggtgataagtgataattcctgccccttgtgccaagaggataatgaaacagccaatcacctttttggttactgcaggtttgctaaggaagtttggaacctggcaaatatagtttccccagtcaactgggaagagggctaccttaaggtgttcaaggagctcatccttttacaacaatttaatggtttattgtttgaaaaaatcattactatttattggcaaatctggaaggctatgaatgatgttattttccgcaatgcatcacctagtccaaaacctgtcacttcagcagctgctatagtgcaaaataactctgatcttcctaccttctctagcaccgggttgccatcccctcatccaaatacaatcaaatggcaatctcctgctaaatactatgttaaaaacaactttgatggctctgtctggactgagacccgtgaagttgaccactacactgatactgaatgggagcccattattgaacttactgtccctagaaccaaccacactgacacatggaaggattgcgtgtcagcgataacaattactgatgaaatttcagcgacagtgaaagcaaaattcacttcaaatgctgatgatgacgatgcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

223

Amino Acids

25.64

Weight (kDa)

5.42

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 2 - 52 1.9e-13 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020066)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001147.1_g000006 Rmu_sc0009047.1_g000002
rosa_samantha Rh1AG068000 Rh1AG068300 Rh1AG068500 Rh7AG041800 Rh7DG041900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 126
AciI CCGC 1 cut(s) 313
AcsI RAATTY 2 cut(s) 615, 638
AcuI CTGAAG 1 cut(s) 330
AfiI CCNNNNNNNGG 1 cut(s) 45
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 4 cut(s) 214, 257, 542, 648
AjnI CCWGG 1 cut(s) 159
AloI GAACNNNNNNTCC 2 cut(s) 534, 566
AluBI AGCT 3 cut(s) 9, 220, 353
AluI AGCT 3 cut(s) 9, 220, 353
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 55, 485
AlwNI CAGNNNCTG 1 cut(s) 353
ApeKI GCWGC 2 cut(s) 350, 353
ApoI RAATTY 2 cut(s) 615, 638
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 3 cut(s) 76, 110, 315
AxyI CCTNAGG 1 cut(s) 20
BanII GRGCYC 2 cut(s) 222, 534
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 340, 362
BccI CCATC 2 cut(s) 415, 467
BciT130I CCWGG 1 cut(s) 161
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 55, 485
BfaI CTAG 3 cut(s) 327, 393, 555
BfmI CTRYAG 2 cut(s) 133, 357
BfrI CTTAAG 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 126
BisI GCNGC 2 cut(s) 351, 354
BlsI GCNGC 2 cut(s) 352, 355
Bme1390I CCNGG 2 cut(s) 161, 400
BmiI GGNNCC 2 cut(s) 158, 531
BmrFI CCNGG 2 cut(s) 161, 400
BmrI ACTGGG 2 cut(s) 173, 196
BmsI GCATC 2 cut(s) 329, 655
BmuI ACTGGG 2 cut(s) 173, 196
BpiI GAAGAC 1 cut(s) 34
Bpu10I CCTNAGC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 400
BsaI GGTCTC 2 cut(s) 55, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse1I ACTGG 2 cut(s) 179, 191
Bse21I CCTNAGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 322
BseBI CCWGG 1 cut(s) 161
BseGI GGATG 3 cut(s) 222, 407, 415
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 322
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 2 cut(s) 179, 191
BseXI GCAGC 2 cut(s) 340, 362
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 2 cut(s) 55, 485
BsmFI GGGAC 1 cut(s) 536
Bso31I GGTCTC 2 cut(s) 55, 485
Bsp1286I GDGCHC 2 cut(s) 222, 534
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 1 cut(s) 313
BspCNI CTCAG 1 cut(s) 481
BspLI GGNNCC 2 cut(s) 158, 531
BspMAI CTGCAG 1 cut(s) 137
BspMI ACCTGC 1 cut(s) 126
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 55, 485
BsrDI GCAATG 1 cut(s) 322
BsrI ACTGG 2 cut(s) 179, 191
BssMI GATC 1 cut(s) 376
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 2 cut(s) 550, 628
Bst6I CTCTTC 1 cut(s) 186
BstAFI CTTAAG 1 cut(s) 203
BstDEI CTNAG 3 cut(s) 20, 144, 489
BstF5I GGATG 3 cut(s) 222, 407, 415
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 2 cut(s) 55, 485
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 2 cut(s) 159, 398
BstSFI CTRYAG 2 cut(s) 133, 357
BstV1I GCAGC 2 cut(s) 340, 362
BstV2I GAAGAC 1 cut(s) 34
BstXI CCANNNNNNTGG 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 222, 407, 415
BtsIMutI CAGTG 3 cut(s) 513, 567, 633
BveI ACCTGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 353
CviAII CATG 1 cut(s) 576
CviJI RGCY 8 cut(s) 9, 113, 198, 220, 293, 353, 477, 532
CviKI_1 RGCY 8 cut(s) 9, 113, 198, 220, 293, 353, 477, 532
DdeI CTNAG 3 cut(s) 20, 144, 489
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 222, 534
Eco31I GGTCTC 2 cut(s) 55, 485
Eco53kI GAGCTC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 222, 534
FaeI CATG 1 cut(s) 579
FaiI YATR 5 cut(s) 170, 296, 359, 456, 577
FaqI GGGAC 1 cut(s) 536
FatI CATG 1 cut(s) 575
Fnu4HI GCNGC 2 cut(s) 351, 354
FokI GGATG 3 cut(s) 209, 394, 402
FriOI GRGCYC 2 cut(s) 222, 534
Fsp4HI GCNGC 2 cut(s) 351, 354
FspBI CTAG 3 cut(s) 327, 393, 555
GluI GCNGC 2 cut(s) 351, 354
HapII CCGG 1 cut(s) 399
Hin1II CATG 1 cut(s) 579
HincII GTYRAC 2 cut(s) 184, 505
HindII GTYRAC 2 cut(s) 184, 505
HpaII CCGG 1 cut(s) 399
HphI GGTGA 3 cut(s) 76, 110, 315
Hpy166II GTNNAC 2 cut(s) 184, 505
Hpy188I TCNGA 1 cut(s) 376
Hpy188III TCNNGA 2 cut(s) 286, 484
Hpy8I GTNNAC 2 cut(s) 184, 505
HpyAV CCTTC 3 cut(s) 283, 397, 574
HpyCH4III ACNGT 2 cut(s) 550, 628
HpyCH4V TGCA 3 cut(s) 135, 320, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
HpyF3I CTNAG 3 cut(s) 20, 144, 489
Hsp92II CATG 1 cut(s) 579
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 3 cut(s) 21, 217, 529
Lsp1109I GCAGC 2 cut(s) 340, 362
LweI GCATC 2 cut(s) 329, 655
MaeI CTAG 3 cut(s) 327, 393, 555
MaeIII GTNAC 2 cut(s) 128, 340
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 3 cut(s) 39, 203, 371
MhlI GDGCHC 2 cut(s) 222, 534
MluCI AATT 5 cut(s) 76, 236, 603, 615, 638
MnlI CCTC 3 cut(s) 91, 187, 423
Mph1103I ATGCAT 1 cut(s) 322
MseI TTAA 3 cut(s) 204, 240, 459
MspA1I CMGCKG 1 cut(s) 353
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 2 cut(s) 161, 400
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 1 cut(s) 90
NciI CCSGG 1 cut(s) 400
NdeII GATC 1 cut(s) 376
NlaIII CATG 1 cut(s) 579
NlaIV GGNNCC 2 cut(s) 158, 531
NmuCI GTSAC 1 cut(s) 340
NsiI ATGCAT 1 cut(s) 322
PkrI GCNGC 2 cut(s) 352, 355
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 158, 531
PstI CTGCAG 1 cut(s) 137
PstNI CAGNNNCTG 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 3 cut(s) 204, 240, 459
SatI GCNGC 2 cut(s) 351, 354
Sau3AI GATC 1 cut(s) 376
ScrFI CCNGG 2 cut(s) 161, 400
SduI GDGCHC 2 cut(s) 222, 534
SfaNI GCATC 2 cut(s) 329, 655
SfcI CTRYAG 2 cut(s) 133, 357
SmlI CTYRAG 2 cut(s) 52, 203
SmoI CTYRAG 2 cut(s) 52, 203
Sse9I AATT 5 cut(s) 76, 236, 603, 615, 638
SsiI CCGC 1 cut(s) 313
SspMI CTAG 3 cut(s) 327, 393, 555
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 159, 398
TaaI ACNGT 2 cut(s) 550, 628
TasI AATT 5 cut(s) 76, 236, 603, 615, 638
Tru1I TTAA 3 cut(s) 204, 240, 459
Tru9I TTAA 3 cut(s) 204, 240, 459
TscAI CASTG 3 cut(s) 520, 574, 633
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 350, 353
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 3 cut(s) 120, 311, 627
TspRI CASTG 3 cut(s) 520, 574, 633
Vha464I CTTAAG 1 cut(s) 203
XapI RAATTY 2 cut(s) 615, 638
XspI CTAG 3 cut(s) 327, 393, 555
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.