Rmu_sc0001154.1_g000011

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001154.1
OG0020692
Physical Location & Seq
Reverse (-)
83991 .. 84635
645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ctggagtcgtgggtgttcgcctgccacgagctcctcaagcgcgtgaaccgccgcgtcgagtccagcgaattcgaaaaggccgccaccgactgttacctgatcgagcgcgtctggaagcttctggcggaaatcgaggacctccacctgttaatggacccggaggactttcttcggctcaagaaccagttgaagatcaagacggtggacgatacggagtcgttttgcttcaggtcgaagggactggtggagatcacaaggctgtgtaaggacttgaagcacaaggttccgaacgtgctgggagtggaggtggacccgatgggcgggccgaggatccaggaggcggcgatgaggctctactcggagaaaaccgggtcggagaagatccatctccttcaggcgatgcagactatagaatcggcggtgaaacgtttcttcttcgcgtacaagcaggtgctagtggtgttgatggggagcttggaggcgaaagggaaccgggtcgtggtgagcccggagtccggcgacgcgctgagtcagatattcctggagccgacgtattttccgagcttggacgccgcgaagacgtttctgggggacggtttgagtcacgaaaacgtttcggagagacggacgcgcaggactaggtag

Protein Analysis

214

Amino Acids

24.52

Weight (kDa)

7.13

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_C PF07014 1 - 198 2e-101 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020692)

Species Orthologous Gene IDs
rosa_laevigata RLG00000027114
rosa_multiflora Rmu_sc0001154.1_g000011 Rmu_sc0001154.1_g000020
rosa_samantha Rh1BG323400 Rh1CG336700 Rh1DG354200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 439
Acc36I ACCTGC 1 cut(s) 439
AccII CGCG 7 cut(s) 42, 54, 108, 440, 524, 575, 631
AciI CCGC 8 cut(s) 49, 52, 81, 125, 321, 341, 419, 573
AclI AACGTT 2 cut(s) 427, 612
AclWI GGATC 3 cut(s) 325, 338, 376
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 2 cut(s) 211, 377
AcyI GRCGYC 1 cut(s) 570
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 5 cut(s) 151, 320, 340, 499, 515
AgsI TTSAA 2 cut(s) 190, 274
AjnI CCWGG 2 cut(s) 333, 540
AluBI AGCT 4 cut(s) 31, 118, 474, 564
AluI AGCT 4 cut(s) 31, 118, 474, 564
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 616
AlwI GGATC 3 cut(s) 325, 338, 376
AoxI GGCC 2 cut(s) 78, 323
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 4 cut(s) 42, 108, 526, 633
AspS9I GGNCC 4 cut(s) 136, 154, 310, 323
AsuC2I CCSGG 4 cut(s) 158, 370, 494, 509
AsuHPI GGTGA 2 cut(s) 433, 514
AsuII TTCGAA 1 cut(s) 72
AvaII GGWCC 3 cut(s) 136, 154, 310
BamHI GGATCC 1 cut(s) 330
BanII GRGCYC 2 cut(s) 33, 509
BauI CACGAG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 3 cut(s) 310, 393, 460
BciT130I CCWGG 2 cut(s) 335, 542
BcnI CCSGG 4 cut(s) 158, 370, 494, 509
BcoDI GTCTC 1 cut(s) 616
BfaI CTAG 2 cut(s) 455, 639
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 439
BisI GCNGC 4 cut(s) 52, 81, 342, 573
BlsI GCNGC 4 cut(s) 53, 82, 343, 574
Bme1390I CCNGG 6 cut(s) 158, 335, 370, 494, 509, 542
Bme18I GGWCC 3 cut(s) 136, 154, 310
BmgT120I GGNCC 4 cut(s) 136, 154, 310, 323
BmiI GGNNCC 6 cut(s) 156, 285, 312, 332, 491, 546
BmrFI CCNGG 6 cut(s) 158, 335, 370, 494, 509, 542
BmsI GCATC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 584
BpmI CTGGAG 2 cut(s) 23, 563
Bpu14I TTCGAA 1 cut(s) 72
BpuEI CTTGAG 2 cut(s) 20, 161
BpuMI CCSGG 4 cut(s) 158, 370, 494, 509
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 326
BsaXI ACNNNNNCTCC 4 cut(s) 291, 321, 536, 566
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 320, 340, 499, 515
Bse1I ACTGG 2 cut(s) 184, 246
BseBI CCWGG 2 cut(s) 335, 542
BseDI CCNNGG 1 cut(s) 326
BseLI CCNNNNNNNGG 5 cut(s) 151, 320, 340, 499, 515
BseMII CTCAG 1 cut(s) 518
BseNI ACTGG 2 cut(s) 184, 246
BseRI GAGGAG 1 cut(s) 23
BseYI CCCAGC 1 cut(s) 295
Bsh1236I CGCG 7 cut(s) 42, 54, 108, 440, 524, 575, 631
BshFI GGCC 2 cut(s) 80, 325
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 5 cut(s) 158, 369, 493, 509, 516
BslFI GGGAC 2 cut(s) 252, 605
BslI CCNNNNNNNGG 5 cut(s) 151, 320, 340, 499, 515
BsmAI GTCTC 1 cut(s) 616
BsmBI CGTCTC 1 cut(s) 616
BsmFI GGGAC 2 cut(s) 252, 605
BsnI GGCC 2 cut(s) 80, 325
Bsp119I TTCGAA 1 cut(s) 72
Bsp1286I GDGCHC 2 cut(s) 33, 509
Bsp143I GATC 5 cut(s) 99, 192, 249, 330, 381
BspACI CCGC 8 cut(s) 49, 52, 81, 125, 321, 341, 419, 573
BspANI GGCC 2 cut(s) 80, 325
BspCNI CTCAG 1 cut(s) 519
BspFNI CGCG 7 cut(s) 42, 54, 108, 440, 524, 575, 631
BspLI GGNNCC 6 cut(s) 156, 285, 312, 332, 491, 546
BspMI ACCTGC 1 cut(s) 439
BspPI GGATC 3 cut(s) 325, 338, 376
BspT104I TTCGAA 1 cut(s) 72
BsrI ACTGG 2 cut(s) 184, 246
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 5 cut(s) 99, 192, 249, 330, 381
BssNI GRCGYC 1 cut(s) 570
BssSI CACGAG 1 cut(s) 26
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 2 cut(s) 335, 542
Bst4CI ACNGT 3 cut(s) 92, 202, 596
BstACI GRCGYC 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 72
BstC8I GCNNGC 2 cut(s) 22, 323
BstDEI CTNAG 1 cut(s) 527
BstFNI CGCG 7 cut(s) 42, 54, 108, 440, 524, 575, 631
BstHHI GCGC 4 cut(s) 42, 108, 526, 633
BstKTI GATC 5 cut(s) 102, 195, 252, 333, 384
BstMAI GTCTC 1 cut(s) 616
BstMBI GATC 5 cut(s) 99, 192, 249, 330, 381
BstMWI GCNNNNNNNGC 2 cut(s) 37, 48
BstNI CCWGG 2 cut(s) 335, 542
BstSCI CCNGG 6 cut(s) 156, 333, 368, 492, 507, 540
BstSFI CTRYAG 1 cut(s) 408
BstUI CGCG 7 cut(s) 42, 54, 108, 440, 524, 575, 631
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 2 cut(s) 330, 381
BstYI RGATCY 2 cut(s) 330, 381
BsuRI GGCC 2 cut(s) 80, 325
BtgZI GCGATG 2 cut(s) 359, 413
BveI ACCTGC 1 cut(s) 439
Cac8I GCNNGC 2 cut(s) 22, 323
CfoI GCGC 4 cut(s) 42, 108, 526, 633
Cfr13I GGNCC 4 cut(s) 136, 154, 310, 323
CseI GACGC 5 cut(s) 43, 97, 530, 578, 637
Csp6I GTAC 1 cut(s) 442
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 1 cut(s) 527
DpnI GATC 5 cut(s) 101, 194, 251, 332, 383
DpnII GATC 5 cut(s) 99, 192, 249, 330, 381
EciI GGCGGA 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 33, 509
Eco47I GGWCC 3 cut(s) 136, 154, 310
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 2 cut(s) 211, 377
EcoICRI GAGCTC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 333, 540
EcoT38I GRGCYC 2 cut(s) 33, 509
Esp3I CGTCTC 1 cut(s) 616
FaiI YATR 1 cut(s) 410
FaqI GGGAC 2 cut(s) 252, 605
FauI CCCGC 1 cut(s) 314
Fnu4HI GCNGC 4 cut(s) 52, 81, 342, 573
FriOI GRGCYC 2 cut(s) 33, 509
Fsp4HI GCNGC 4 cut(s) 52, 81, 342, 573
FspBI CTAG 2 cut(s) 455, 639
GlaI GCGC 4 cut(s) 41, 107, 525, 632
GluI GCNGC 4 cut(s) 52, 81, 342, 573
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 2 cut(s) 23, 563
HaeIII GGCC 2 cut(s) 80, 325
HapII CCGG 5 cut(s) 158, 369, 493, 509, 516
HgaI GACGC 5 cut(s) 43, 97, 530, 578, 637
HhaI GCGC 4 cut(s) 42, 108, 526, 633
Hin1I GRCGYC 1 cut(s) 570
Hin6I GCGC 4 cut(s) 40, 106, 524, 631
HinP1I GCGC 4 cut(s) 40, 106, 524, 631
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 7 cut(s) 5, 59, 215, 413, 512, 529, 601
HpaII CCGG 5 cut(s) 158, 369, 493, 509, 516
HphI GGTGA 2 cut(s) 433, 514
Hpy166II GTNNAC 3 cut(s) 46, 205, 310
Hpy188I TCNGA 6 cut(s) 288, 361, 376, 534, 561, 619
Hpy188III TCNNGA 4 cut(s) 112, 178, 196, 605
Hpy8I GTNNAC 3 cut(s) 46, 205, 310
Hpy99I CGWCG 3 cut(s) 59, 524, 553
HpyAV CCTTC 2 cut(s) 229, 401
HpyCH4III ACNGT 3 cut(s) 92, 202, 596
HpyCH4IV ACGT 5 cut(s) 291, 427, 551, 581, 612
HpyCH4V TGCA 1 cut(s) 403
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 48
HpyF3I CTNAG 1 cut(s) 527
HpySE526I ACGT 5 cut(s) 291, 427, 551, 581, 612
Hsp92I GRCGYC 1 cut(s) 570
HspAI GCGC 4 cut(s) 40, 106, 524, 631
Kzo9I GATC 5 cut(s) 99, 192, 249, 330, 381
LmnI GCTCC 3 cut(s) 36, 471, 544
LweI GCATC 1 cut(s) 390
MaeI CTAG 2 cut(s) 455, 639
MaeII ACGT 5 cut(s) 291, 427, 551, 581, 612
MaeIII GTNAC 2 cut(s) 92, 602
MalI GATC 5 cut(s) 101, 194, 251, 332, 383
MboI GATC 5 cut(s) 99, 192, 249, 330, 381
MboII GAAGA 6 cut(s) 161, 202, 391, 424, 427, 589
MflI RGATCY 2 cut(s) 330, 381
MhlI GDGCHC 2 cut(s) 33, 509
MluCI AATT 1 cut(s) 68
MlyI GAGTC 6 cut(s) 14, 68, 224, 521, 538, 610
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 9 cut(s) 44, 127, 149, 154, 298, 321, 331, 342, 472
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 1 cut(s) 149
MspI CCGG 5 cut(s) 158, 369, 493, 509, 516
MspR9I CCNGG 6 cut(s) 158, 335, 370, 494, 509, 542
MvaI CCWGG 2 cut(s) 335, 542
MvnI CGCG 7 cut(s) 42, 54, 108, 440, 524, 575, 631
MwoI GCNNNNNNNGC 2 cut(s) 37, 48
NciI CCSGG 4 cut(s) 158, 370, 494, 509
NdeII GATC 5 cut(s) 99, 192, 249, 330, 381
NlaIV GGNNCC 6 cut(s) 156, 285, 312, 332, 491, 546
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 602
NspV TTCGAA 1 cut(s) 72
PaqCI CACCTGC 1 cut(s) 439
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 63, 557
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 413
PfoI TCCNGGA 2 cut(s) 333, 540
PkrI GCNGC 4 cut(s) 53, 82, 343, 574
PleI GAGTC 6 cut(s) 13, 67, 223, 520, 537, 609
PpsI GAGTC 6 cut(s) 13, 67, 223, 520, 537, 609
PpuMI RGGWCCY 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 33
Psp1406I AACGTT 2 cut(s) 427, 612
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 333, 540
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 2 cut(s) 333, 540
PspN4I GGNNCC 6 cut(s) 156, 285, 312, 332, 491, 546
PspPI GGNCC 4 cut(s) 136, 154, 310, 323
PspPPI RGGWCCY 1 cut(s) 136
PsuI RGATCY 2 cut(s) 330, 381
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 4 cut(s) 52, 81, 342, 573
Sau3AI GATC 5 cut(s) 99, 192, 249, 330, 381
Sau96I GGNCC 4 cut(s) 136, 154, 310, 323
SchI GAGTC 6 cut(s) 14, 68, 224, 521, 538, 610
ScrFI CCNGG 6 cut(s) 158, 335, 370, 494, 509, 542
SduI GDGCHC 2 cut(s) 33, 509
SfaNI GCATC 1 cut(s) 390
SfcI CTRYAG 1 cut(s) 408
SfuI TTCGAA 1 cut(s) 72
SinI GGWCC 3 cut(s) 136, 154, 310
SmlI CTYRAG 2 cut(s) 35, 176
SmoI CTYRAG 2 cut(s) 35, 176
Sse9I AATT 1 cut(s) 68
SsiI CCGC 8 cut(s) 49, 52, 81, 125, 321, 341, 419, 573
SspMI CTAG 2 cut(s) 455, 639
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 6 cut(s) 156, 333, 368, 492, 507, 540
TaaI ACNGT 3 cut(s) 92, 202, 596
TaiI ACGT 5 cut(s) 294, 430, 554, 584, 615
TaqI TCGA 5 cut(s) 57, 72, 102, 132, 233
TasI AATT 1 cut(s) 68
TauI GCSGC 4 cut(s) 54, 83, 344, 575
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TseFI GTSAC 1 cut(s) 602
Tsp45I GTSAC 1 cut(s) 602
TspGWI ACGGA 2 cut(s) 227, 640
VpaK11BI GGWCC 3 cut(s) 136, 154, 310
XapI RAATTY 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 2 cut(s) 455, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.