Rmu_sc0001154.1_g000020

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001154.1
OG0020692
Physical Location & Seq
Reverse (-)
126032 .. 127289
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
atggacccggaggactttctccggctcaagaaccagttgaagatcgaaatggtcgacgatacggagtcgttttgcttcagatcgaagggactagtggagatcactaggctctgcaaggacttgaagcacaaggtaccgaacgtgctaggagtggaggtggatcccatgggcaggccgaggatccaggaggcggcgatgaggctctactcgaaaaaaaccggttcagagaagatccatctccttcaggcgatgcagactatcgagtcggcggtgaaacgtttcttctttgcatacaagcaggtgctggtggtgttgatagggagcttggaggcgaaacggaaccgggtcgtggtgggcccagaatccagtgacgcgctgagccagatattcttggagctgacgtattttcctagcttggacaaggcgaaggcgtttatgggggacgagccctcaactgggcctgtttccaacaagtccgacgggttacagcggtgggtattagtgtattactgccttccttcttctcatcctcacggtacccagtttccatcgcaaccaagctaccgtccacctcctcctcaatttccattccaaccgagttactcaccaccactactgccttcccacacgatcatcaatgggtatgttttccctcatcaatttccatcaacccatacttaccattcacctgatactgacccaaatccctggatctatccttctatttcacaaatccattcctatgacccatcggttcccagtgttaatttacacttcgtctccaccaaaaccatgcctccaccacctatttaccattctgctccttcacctcccatttgcgagcaaccacatacaatttcatatccagataattgggaggtcccaattgttgactctggtagctgctctcttgtcacgccccgaattttgaattaa

Protein Analysis

311

Amino Acids

35.05

Weight (kDa)

6.86

Isoelectric Point (pI)

56.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_C PF07014 1 - 148 1e-67 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020692)

Species Orthologous Gene IDs
rosa_laevigata RLG00000027114
rosa_multiflora Rmu_sc0001154.1_g000011 Rmu_sc0001154.1_g000020
rosa_samantha Rh1BG323400 Rh1CG336700 Rh1DG354200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 289
AasI GACNNNNNNGTC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 289
Acc65I GGTACC 2 cut(s) 133, 536
AccB1I GGYRCC 2 cut(s) 133, 536
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 374
AciI CCGC 3 cut(s) 191, 269, 490
AclI AACGTT 1 cut(s) 277
AclWI GGATC 6 cut(s) 155, 168, 175, 188, 226, 719
AcsI RAATTY 1 cut(s) 924
AcuI CTGAAG 2 cut(s) 61, 227
AfaI GTAC 2 cut(s) 135, 538
AfiI CCNNNNNNNGG 5 cut(s) 171, 190, 349, 455, 456
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 3 cut(s) 40, 124, 931
AhlI ACTAGT 1 cut(s) 91
AjnI CCWGG 2 cut(s) 183, 707
AluBI AGCT 5 cut(s) 324, 397, 414, 561, 903
AluI AGCT 5 cut(s) 324, 397, 414, 561, 903
Alw26I GTCTC 1 cut(s) 784
AlwI GGATC 6 cut(s) 155, 168, 175, 188, 226, 719
AlwNI CAGNNNCTG 1 cut(s) 304
AoxI GGCC 3 cut(s) 173, 355, 458
ApaI GGGCCC 1 cut(s) 359
ApeKI GCWGC 1 cut(s) 903
ApoI RAATTY 1 cut(s) 924
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 278
Asp718I GGTACC 2 cut(s) 133, 536
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 5 cut(s) 4, 355, 356, 458, 880
AsuC2I CCSGG 2 cut(s) 8, 344
AsuHPI GGTGA 4 cut(s) 283, 597, 678, 819
AvaII GGWCC 2 cut(s) 4, 880
BaeGI GKGCMC 1 cut(s) 359
BamHI GGATCC 2 cut(s) 160, 180
BanI GGYRCC 2 cut(s) 133, 536
BanII GRGCYC 2 cut(s) 359, 450
BbvI GCAGC 1 cut(s) 890
BccI CCATC 4 cut(s) 243, 556, 673, 757
BciT130I CCWGG 2 cut(s) 185, 709
BcnI CCSGG 2 cut(s) 8, 344
BcoDI GTCTC 1 cut(s) 784
BcuI ACTAGT 1 cut(s) 91
BfaI CTAG 4 cut(s) 92, 105, 146, 411
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 2 cut(s) 192, 904
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 2 cut(s) 193, 905
Bme1390I CCNGG 4 cut(s) 8, 185, 344, 709
Bme18I GGWCC 2 cut(s) 4, 880
BmgT120I GGNCC 5 cut(s) 4, 355, 356, 458, 880
BmiI GGNNCC 9 cut(s) 6, 135, 162, 182, 341, 357, 538, 756, 882
BmrFI CCNGG 4 cut(s) 8, 185, 344, 709
BmrI ACTGGG 3 cut(s) 465, 535, 753
BmsI GCATC 1 cut(s) 240
BmuI ACTGGG 3 cut(s) 465, 535, 753
Bpu1102I GCTNAGC 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 2 cut(s) 8, 344
BsaJI CCNNGG 3 cut(s) 165, 176, 707
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 8 cut(s) 141, 171, 386, 416, 764, 781, 794, 811
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 190, 349, 455, 456
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 5 cut(s) 34, 366, 460, 541, 759
BseBI CCWGG 2 cut(s) 185, 709
BseDI CCNNGG 3 cut(s) 165, 176, 707
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 5 cut(s) 171, 190, 349, 455, 456
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 5 cut(s) 34, 366, 460, 541, 759
BseRI GAGGAG 2 cut(s) 564, 567
BseSI GKGCMC 1 cut(s) 359
BseXI GCAGC 1 cut(s) 890
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 3 cut(s) 175, 357, 460
BshNI GGYRCC 2 cut(s) 133, 536
BshTI ACCGGT 1 cut(s) 218
BsiSI CCGG 4 cut(s) 8, 22, 219, 343
BslFI GGGAC 3 cut(s) 102, 455, 866
BslI CCNNNNNNNGG 5 cut(s) 171, 190, 349, 455, 456
BsmAI GTCTC 1 cut(s) 784
BsmBI CGTCTC 1 cut(s) 784
BsmFI GGGAC 3 cut(s) 102, 455, 866
BsnI GGCC 3 cut(s) 175, 357, 460
Bsp120I GGGCCC 1 cut(s) 355
Bsp1286I GDGCHC 2 cut(s) 359, 450
Bsp143I GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
Bsp1720I GCTNAGC 1 cut(s) 377
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 3 cut(s) 191, 269, 490
BspANI GGCC 3 cut(s) 175, 357, 460
BspCNI CTCAG 1 cut(s) 369
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 9 cut(s) 6, 135, 162, 182, 341, 357, 538, 756, 882
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 6 cut(s) 155, 168, 175, 188, 226, 719
BspT107I GGYRCC 2 cut(s) 133, 536
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 5 cut(s) 34, 366, 460, 541, 759
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 3 cut(s) 165, 176, 707
BssMI GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 185, 709
Bst4CI ACNGT 2 cut(s) 536, 566
BstC8I GCNNGC 2 cut(s) 173, 842
BstDEI CTNAG 1 cut(s) 377
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 8 cut(s) 45, 83, 102, 163, 183, 234, 633, 714
BstMAI GTCTC 1 cut(s) 784
BstMBI GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
BstNI CCWGG 2 cut(s) 185, 709
BstSCI CCNGG 4 cut(s) 6, 183, 342, 707
BstSLI GKGCMC 1 cut(s) 359
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 890
BstX2I RGATCY 4 cut(s) 160, 180, 231, 711
BstXI CCANNNNNNTGG 2 cut(s) 708, 873
BstYI RGATCY 4 cut(s) 160, 180, 231, 711
BsuRI GGCC 3 cut(s) 175, 357, 460
BtgI CCRYGG 1 cut(s) 165
BtgZI GCGATG 3 cut(s) 209, 263, 534
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 2 cut(s) 373, 766
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 173, 842
CaiI CAGNNNCTG 1 cut(s) 304
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 5 cut(s) 4, 355, 356, 458, 880
CseI GACGC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 134, 537
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 2 cut(s) 166, 793
CviQI GTAC 2 cut(s) 134, 537
DdeI CTNAG 1 cut(s) 377
DpnI GATC 8 cut(s) 44, 82, 101, 162, 182, 233, 632, 713
DpnII GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
DrdI GACNNNNNNGTC 1 cut(s) 262
DseDI GACNNNNNNGTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 359, 450
Eco47I GGWCC 2 cut(s) 4, 880
Eco57I CTGAAG 2 cut(s) 61, 227
EcoO109I RGGNCCY 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 183, 707
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 2 cut(s) 359, 450
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 784
FaeI CATG 2 cut(s) 169, 796
FaiI YATR 9 cut(s) 167, 292, 437, 645, 675, 744, 794, 852, 862
FaqI GGGAC 3 cut(s) 102, 455, 866
FatI CATG 2 cut(s) 165, 792
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 192, 904
FokI GGATG 1 cut(s) 513
FriOI GRGCYC 2 cut(s) 359, 450
Fsp4HI GCNGC 2 cut(s) 192, 904
FspBI CTAG 4 cut(s) 92, 105, 146, 411
GlaI GCGC 1 cut(s) 375
GluI GCNGC 2 cut(s) 192, 904
HaeIII GGCC 3 cut(s) 175, 357, 460
HapII CCGG 4 cut(s) 8, 22, 219, 343
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 376
Hin1II CATG 2 cut(s) 169, 796
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 2 cut(s) 55, 892
HindII GTYRAC 2 cut(s) 55, 892
HinfI GANTC 4 cut(s) 65, 263, 362, 893
HpaII CCGG 4 cut(s) 8, 22, 219, 343
HphI GGTGA 4 cut(s) 283, 597, 678, 819
Hpy166II GTNNAC 3 cut(s) 55, 569, 892
Hpy188I TCNGA 3 cut(s) 80, 226, 478
Hpy188III TCNNGA 2 cut(s) 28, 866
Hpy8I GTNNAC 3 cut(s) 55, 569, 892
Hpy99I CGWCG 2 cut(s) 59, 482
HpyAV CCTTC 8 cut(s) 79, 251, 421, 524, 528, 630, 729, 834
HpyCH4III ACNGT 2 cut(s) 536, 566
HpyCH4IV ACGT 3 cut(s) 141, 277, 401
HpyCH4V TGCA 3 cut(s) 114, 253, 290
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 3 cut(s) 141, 277, 401
Hsp92II CATG 2 cut(s) 169, 796
HspAI GCGC 1 cut(s) 374
KpnI GGTACC 2 cut(s) 137, 540
Kzo9I GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
LmnI GCTCC 3 cut(s) 321, 394, 826
Lsp1109I GCAGC 1 cut(s) 890
LweI GCATC 1 cut(s) 240
MaeI CTAG 4 cut(s) 92, 105, 146, 411
MaeII ACGT 3 cut(s) 141, 277, 401
MaeIII GTNAC 4 cut(s) 368, 483, 599, 913
MalI GATC 8 cut(s) 44, 82, 101, 162, 182, 233, 632, 713
MboI GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
MboII GAAGA 4 cut(s) 52, 241, 274, 513
MfeI CAATTG 1 cut(s) 885
MflI RGATCY 4 cut(s) 160, 180, 231, 711
MhlI GDGCHC 2 cut(s) 359, 450
MluCI AATT 8 cut(s) 581, 659, 766, 855, 871, 885, 924, 931
MlyI GAGTC 3 cut(s) 74, 272, 887
MmeI TCCRAC 3 cut(s) 492, 501, 616
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 2 cut(s) 765, 934
MslI CAYNNNNRTG 1 cut(s) 741
MspA1I CMGCKG 1 cut(s) 490
MspI CCGG 4 cut(s) 8, 22, 219, 343
MspR9I CCNGG 4 cut(s) 8, 185, 344, 709
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 2 cut(s) 185, 709
MvnI CGCG 1 cut(s) 374
NciI CCSGG 2 cut(s) 8, 344
NcoI CCATGG 1 cut(s) 165
NdeII GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
NlaIII CATG 2 cut(s) 169, 796
NlaIV GGNNCC 9 cut(s) 6, 135, 162, 182, 341, 357, 538, 756, 882
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 2 cut(s) 368, 913
PaqCI CACCTGC 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 2 cut(s) 193, 905
PleI GAGTC 3 cut(s) 73, 271, 887
PpsI GAGTC 3 cut(s) 73, 271, 887
PpuMI RGGWCCY 1 cut(s) 880
Psp1406I AACGTT 1 cut(s) 277
Psp5II RGGWCCY 1 cut(s) 880
Psp6I CCWGG 2 cut(s) 183, 707
PspGI CCWGG 2 cut(s) 183, 707
PspN4I GGNNCC 9 cut(s) 6, 135, 162, 182, 341, 357, 538, 756, 882
PspOMI GGGCCC 1 cut(s) 355
PspPI GGNCC 5 cut(s) 4, 355, 356, 458, 880
PspPPI RGGWCCY 1 cut(s) 880
PstNI CAGNNNCTG 1 cut(s) 304
PsuI RGATCY 4 cut(s) 160, 180, 231, 711
RsaI GTAC 2 cut(s) 135, 538
RsaNI GTAC 2 cut(s) 134, 537
RseI CAYNNNNRTG 1 cut(s) 741
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 765, 934
SatI GCNGC 2 cut(s) 192, 904
Sau3AI GATC 8 cut(s) 42, 80, 99, 160, 180, 231, 630, 711
Sau96I GGNCC 5 cut(s) 4, 355, 356, 458, 880
SchI GAGTC 3 cut(s) 74, 272, 887
ScrFI CCNGG 4 cut(s) 8, 185, 344, 709
SduI GDGCHC 2 cut(s) 359, 450
SfaNI GCATC 1 cut(s) 240
SinI GGWCC 2 cut(s) 4, 880
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SpeI ACTAGT 1 cut(s) 91
Sse9I AATT 8 cut(s) 581, 659, 766, 855, 871, 885, 924, 931
SsiI CCGC 3 cut(s) 191, 269, 490
SspMI CTAG 4 cut(s) 92, 105, 146, 411
StyD4I CCNGG 4 cut(s) 6, 183, 342, 707
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 536, 566
TaiI ACGT 3 cut(s) 144, 280, 404
TaqI TCGA 5 cut(s) 45, 54, 83, 209, 261
TasI AATT 8 cut(s) 581, 659, 766, 855, 871, 885, 924, 931
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 2 cut(s) 765, 934
Tru9I TTAA 2 cut(s) 765, 934
TscAI CASTG 2 cut(s) 373, 766
TseFI GTSAC 2 cut(s) 368, 913
TseI GCWGC 1 cut(s) 903
Tsp45I GTSAC 2 cut(s) 368, 913
TspDTI ATGAA 1 cut(s) 849
TspGWI ACGGA 2 cut(s) 77, 352
TspRI CASTG 2 cut(s) 373, 766
VpaK11BI GGWCC 2 cut(s) 4, 880
XapI RAATTY 1 cut(s) 924
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 4 cut(s) 92, 105, 146, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.