Rmu_sc0001196.1_g000011

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001196.1
OG0023061
Physical Location & Seq
Reverse (-)
62411 .. 64123
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001196.1_g000011.1.cds

Sequence Viewer

Length: 525 bp
atgatgcccagatgctccgttcaatcctcagatgttcagttctttcagaccataactggccttcaacaactcgccgaaacccaccgtttcaaggcctggttattagatcagtttggagtactccacgatgggaagcagccttatcccggagcaatttcaacattgaagaagctagcaaatagtggtgcaaagatggttattataaggactctggctgccaaatatgaagagctggggggtgaagtgaaatggatgggcaagcctgataagattatctataaatcagccatggccttagttggtgtagaccctattgattctattgcagtgggcgattctcttcatcatgacatcaagggtgcaaatgcagctgcaatccaatcagttttaatcactggtggaatccatgcaactgaacttggacttggtagttttgcagacgtagcagatttaccttctgtgcaagctcttgcttccaaatatgatgcttacccatcgtatgtgttacctgcctttacatggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

174

Amino Acids

18.72

Weight (kDa)

6.5

Isoelectric Point (pI)

29.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023061)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8270497.1_g000001 Rmu_sc0001196.1_g000011
rosa_samantha Rh2DG007000 Rh7BG425500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 306
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 3 cut(s) 91, 146, 519
AgsI TTSAA 5 cut(s) 23, 65, 91, 159, 166
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AluBI AGCT 4 cut(s) 172, 232, 371, 467
AluI AGCT 4 cut(s) 172, 232, 371, 467
AoxI GGCC 3 cut(s) 58, 93, 291
ApeKI GCWGC 4 cut(s) 136, 215, 368, 371
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 251
AsuNHI GCTAGC 1 cut(s) 172
BbvI GCAGC 4 cut(s) 148, 202, 358, 380
BccI CCATC 4 cut(s) 122, 187, 247, 502
BciT130I CCWGG 1 cut(s) 97
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 4 cut(s) 137, 216, 369, 372
BlsI GCNGC 4 cut(s) 138, 217, 370, 373
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 2 cut(s) 97, 147
BmrFI CCNGG 2 cut(s) 97, 147
BmsI GCATC 2 cut(s) 2, 475
BmtI GCTAGC 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 288
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 146, 519
Bse1I ACTGG 2 cut(s) 61, 400
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 91, 146, 519
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 61, 400
BseXI GCAGC 4 cut(s) 148, 202, 358, 380
BseYI CCCAGC 1 cut(s) 232
BshFI GGCC 3 cut(s) 60, 95, 293
BsiSI CCGG 1 cut(s) 147
BslI CCNNNNNNNGG 3 cut(s) 91, 146, 519
BsnI GGCC 3 cut(s) 60, 95, 293
Bsp143I GATC 1 cut(s) 106
Bsp19I CCATGG 1 cut(s) 288
BspANI GGCC 3 cut(s) 60, 95, 293
BspCNI CTCAG 1 cut(s) 41
BspHI TCATGA 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 517
BspOI GCTAGC 1 cut(s) 176
BspQI GCTCTTC 1 cut(s) 222
BsrI ACTGG 2 cut(s) 61, 400
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 2 cut(s) 222, 345
BstC8I GCNNGC 3 cut(s) 174, 260, 465
BstDEI CTNAG 2 cut(s) 28, 295
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 1 cut(s) 258
BstKTI GATC 1 cut(s) 109
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 368, 443
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 2 cut(s) 95, 145
BstV1I GCAGC 4 cut(s) 148, 202, 358, 380
BsuRI GGCC 3 cut(s) 60, 95, 293
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 1 cut(s) 258
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 2 cut(s) 333, 393
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 3 cut(s) 174, 260, 465
CciI TCATGA 1 cut(s) 346
Csp6I GTAC 1 cut(s) 119
CviAII CATG 4 cut(s) 289, 347, 407, 519
CviQI GTAC 1 cut(s) 119
DdeI CTNAG 2 cut(s) 28, 295
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
Eam1104I CTCTTC 2 cut(s) 222, 345
EarI CTCTTC 2 cut(s) 222, 345
Eco130I CCWWGG 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 4 cut(s) 292, 350, 410, 522
FatI CATG 4 cut(s) 288, 346, 406, 518
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 4 cut(s) 137, 216, 369, 372
FokI GGATG 1 cut(s) 265
Fsp4HI GCNGC 4 cut(s) 137, 216, 369, 372
FspBI CTAG 1 cut(s) 173
GluI GCNGC 4 cut(s) 137, 216, 369, 372
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 3 cut(s) 60, 95, 293
HapII CCGG 1 cut(s) 147
Hin1II CATG 4 cut(s) 292, 350, 410, 522
HinfI GANTC 4 cut(s) 208, 317, 335, 402
HpaII CCGG 1 cut(s) 147
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 2 cut(s) 31, 48
Hpy188III TCNNGA 1 cut(s) 347
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 71, 465
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 8 cut(s) 188, 326, 362, 368, 374, 410, 437, 463
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 443
HpyF3I CTNAG 2 cut(s) 28, 295
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 4 cut(s) 292, 350, 410, 522
Kzo9I GATC 1 cut(s) 106
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 2 cut(s) 20, 149
LpnPI CCDG 9 cut(s) 22, 42, 82, 109, 160, 197, 218, 276, 381
Lsp1109I GCAGC 4 cut(s) 148, 202, 358, 380
LweI GCATC 2 cut(s) 2, 475
MaeI CTAG 1 cut(s) 173
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 504
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 3 cut(s) 178, 239, 332
MluCI AATT 1 cut(s) 153
MlyI GAGTC 1 cut(s) 202
MnlI CCTC 1 cut(s) 37
MseI TTAA 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 2 cut(s) 97, 147
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 368, 443
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 288
NdeII GATC 1 cut(s) 106
NheI GCTAGC 1 cut(s) 172
NlaIII CATG 4 cut(s) 292, 350, 410, 522
PagI TCATGA 1 cut(s) 346
PceI AGGCCT 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 222
PfeI GAWTC 3 cut(s) 317, 335, 402
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 4 cut(s) 138, 217, 370, 373
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 4 cut(s) 137, 216, 369, 372
Sau3AI GATC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 2 cut(s) 97, 147
SetI ASST 7 cut(s) 174, 234, 373, 444, 457, 469, 511
SfaNI GCATC 2 cut(s) 2, 475
Sse9I AATT 1 cut(s) 153
SseBI AGGCCT 1 cut(s) 95
SspMI CTAG 1 cut(s) 173
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 95, 145
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 1 cut(s) 444
TasI AATT 1 cut(s) 153
TatI WGTACW 1 cut(s) 118
TfiI GAWTC 3 cut(s) 317, 335, 402
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 2 cut(s) 333, 400
TseI GCWGC 4 cut(s) 136, 215, 368, 371
TspDTI ATGAA 2 cut(s) 240, 332
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 2 cut(s) 333, 400
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 1 cut(s) 173
ZrmI AGTACT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.