Rh7BG425500

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0023061
Physical Location & Seq
Reverse (-)
48499200 .. 48502347
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG425500.1

Sequence Viewer

Length: 237 bp
ATGGCCTTTGTTGGTGTAGACCTTGTTGATTCTATGGCAGTGAGTGACTCTCTTCATCATGACATCAAGGGTGCTAATGCAGCTGCAATGAAATCAATTTTAACCACCGGTGGGATCCATGCAACTGAACAAGGGATTGGTAGTTTTGCAGACATAGCAGATTTATCATCTGTGCAAGCTCTTGCTTCCAAATTCGATGCTTACCCATTATCTGTGTTACCTGCGTTTGCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

78

Amino Acids

8.02

Weight (kDa)

4.58

Isoelectric Point (pI)

25.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_like PF13242 5 - 44 7.8e-07 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023061)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8270497.1_g000001 Rmu_sc0001196.1_g000011
rosa_samantha Rh2DG007000 Rh7BG425500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 18
AclWI GGATC 2 cut(s) 109, 122
AcsI RAATTY 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 111
AgeI ACCGGT 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 2 cut(s) 83, 179
AluI AGCT 2 cut(s) 83, 179
AlwI GGATC 2 cut(s) 109, 122
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 80, 83
ApoI RAATTY 1 cut(s) 191
AsiGI ACCGGT 1 cut(s) 107
BamHI GGATCC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 70, 92
BfuAI ACCTGC 1 cut(s) 229
BisI GCNGC 2 cut(s) 81, 84
BlsI GCNGC 2 cut(s) 82, 85
BmiI GGNNCC 1 cut(s) 116
BmsI GCATC 1 cut(s) 187
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse118I RCCGGY 1 cut(s) 107
Bse3DI GCAATG 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 93
BseXI GCAGC 2 cut(s) 70, 92
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 107
BsiSI CCGG 1 cut(s) 108
BslI CCNNNNNNNGG 1 cut(s) 111
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 114
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 116
BspMI ACCTGC 1 cut(s) 229
BspPI GGATC 2 cut(s) 109, 122
BsrDI GCAATG 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 107
BssMI GATC 1 cut(s) 114
Bst6I CTCTTC 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 177
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 80, 155
BstV1I GCAGC 2 cut(s) 70, 92
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BsuRI GGCC 1 cut(s) 5
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 45
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 177
CciI TCATGA 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 107
CspAI ACCGGT 1 cut(s) 107
CviAII CATG 3 cut(s) 59, 119, 231
CviJI RGCY 3 cut(s) 5, 83, 179
CviKI_1 RGCY 3 cut(s) 5, 83, 179
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
FaeI CATG 3 cut(s) 62, 122, 234
FaiI YATR 5 cut(s) 35, 60, 120, 155, 232
FatI CATG 3 cut(s) 58, 118, 230
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 81, 84
Fsp4HI GCNGC 2 cut(s) 81, 84
GluI GCNGC 2 cut(s) 81, 84
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 108
Hin1II CATG 3 cut(s) 62, 122, 234
HinfI GANTC 2 cut(s) 29, 47
HpaII CCGG 1 cut(s) 108
Hpy166II GTNNAC 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 59
Hpy8I GTNNAC 1 cut(s) 19
HpyCH4V TGCA 6 cut(s) 80, 86, 122, 149, 175, 230
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 155
Hsp92II CATG 3 cut(s) 62, 122, 234
Kzo9I GATC 1 cut(s) 114
LpnPI CCDG 1 cut(s) 121
Lsp1109I GCAGC 2 cut(s) 70, 92
LweI GCATC 1 cut(s) 187
MaeIII GTNAC 2 cut(s) 44, 216
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 1 cut(s) 44
MflI RGATCY 1 cut(s) 114
MluCI AATT 2 cut(s) 96, 191
MlyI GAGTC 1 cut(s) 41
MseI TTAA 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 80, 155
NdeII GATC 1 cut(s) 114
NlaIII CATG 3 cut(s) 62, 122, 234
NlaIV GGNNCC 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 44
PagI TCATGA 1 cut(s) 58
PfeI GAWTC 1 cut(s) 29
PinAI ACCGGT 1 cut(s) 107
PkrI GCNGC 2 cut(s) 82, 85
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 116
PsuI RGATCY 1 cut(s) 114
PvuII CAGCTG 1 cut(s) 83
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 2 cut(s) 81, 84
Sau3AI GATC 1 cut(s) 114
SchI GAGTC 1 cut(s) 41
SetI ASST 4 cut(s) 24, 85, 181, 223
SfaNI GCATC 1 cut(s) 187
SgeI CNNG 9 cut(s) 35, 71, 79, 120, 131, 143, 188, 194, 233
SgrAI CRCCGGYG 1 cut(s) 107
Sse9I AATT 2 cut(s) 96, 191
TaqI TCGA 1 cut(s) 195
TasI AATT 2 cut(s) 96, 191
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 2 cut(s) 80, 83
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 44, 104
TspRI CASTG 1 cut(s) 45
XapI RAATTY 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.