Rh2DG007000

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0023061
Physical Location & Seq
Reverse (-)
425715 .. 426865
1151 bp
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UTR
Exon/CDS
Intron
Rh2DG007000.1

Sequence Viewer

Length: 264 bp
ATGGATAAGATCATCTATAGATCAGCCATGGCCTTAGTTGGTGTAGACCCTATTGATTCTATTGCAGTGGGCGATTCTCTTCATCATGACATCAAGGGCGCAAATGCAGCTGCAATCCAATCAGTTTTAATCACTGGTGGAATCCATGCAACTGAACTTGGACTTGGTAGTTTTGCCGACGTAGCAGATTTATCTTCTGTGCAAGCTCTTGCTTCCAAATGTGATGCTTACCCACCATATGTGTTACCTGCCTTTACATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

87

Amino Acids

9.01

Weight (kDa)

4.63

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_like PF13242 3 - 52 1.9e-10 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023061)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8270497.1_g000001 Rmu_sc0001196.1_g000011
rosa_samantha Rh2DG007000 Rh7BG425500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 258
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AluBI AGCT 2 cut(s) 110, 206
AluI AGCT 2 cut(s) 110, 206
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 2 cut(s) 107, 110
AspLEI GCGC 1 cut(s) 101
BbvI GCAGC 2 cut(s) 97, 119
BfmI CTRYAG 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 2 cut(s) 108, 111
BlsI GCNGC 2 cut(s) 109, 112
BmsI GCATC 1 cut(s) 214
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 139
BseDI CCNNGG 1 cut(s) 27
BseLI CCNNNNNNNGG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 139
BseXI GCAGC 2 cut(s) 97, 119
BshFI GGCC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 258
BsnI GGCC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 9, 20
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 1 cut(s) 32
BspHI TCATGA 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 256
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 2 cut(s) 9, 20
BssT1I CCWWGG 1 cut(s) 27
Bst6I CTCTTC 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 34
BstDSI CCRYGG 1 cut(s) 27
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 2 cut(s) 12, 23
BstMBI GATC 2 cut(s) 9, 20
BstMWI GCNNNNNNNGC 2 cut(s) 107, 182
BstSFI CTRYAG 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 97, 119
BsuRI GGCC 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 27
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 72, 132
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 85
CfoI GCGC 1 cut(s) 101
CviAII CATG 4 cut(s) 28, 86, 146, 258
CviJI RGCY 4 cut(s) 26, 32, 110, 206
CviKI_1 RGCY 4 cut(s) 26, 32, 110, 206
DdeI CTNAG 1 cut(s) 34
DpnI GATC 2 cut(s) 11, 22
DpnII GATC 2 cut(s) 9, 20
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 4 cut(s) 31, 89, 149, 261
FaiI YATR 7 cut(s) 18, 29, 87, 147, 238, 240, 259
FatI CATG 4 cut(s) 27, 85, 145, 257
FauNDI CATATG 1 cut(s) 238
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 108, 111
Fsp4HI GCNGC 2 cut(s) 108, 111
GlaI GCGC 1 cut(s) 100
GluI GCNGC 2 cut(s) 108, 111
HaeIII GGCC 1 cut(s) 32
HhaI GCGC 1 cut(s) 101
Hin1II CATG 4 cut(s) 31, 89, 149, 261
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 3 cut(s) 56, 74, 141
Hpy166II GTNNAC 1 cut(s) 46
Hpy188III TCNNGA 1 cut(s) 86
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 5 cut(s) 65, 107, 113, 149, 202
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 182
HpyF3I CTNAG 1 cut(s) 34
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 4 cut(s) 31, 89, 149, 261
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 2 cut(s) 9, 20
LpnPI CCDG 1 cut(s) 120
Lsp1109I GCAGC 2 cut(s) 97, 119
LweI GCATC 1 cut(s) 214
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 243
MalI GATC 2 cut(s) 11, 22
MboI GATC 2 cut(s) 9, 20
MboII GAAGA 2 cut(s) 71, 186
MseI TTAA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 107, 182
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 238
NdeII GATC 2 cut(s) 9, 20
NlaIII CATG 4 cut(s) 31, 89, 149, 261
PagI TCATGA 1 cut(s) 85
PfeI GAWTC 3 cut(s) 56, 74, 141
PkrI GCNGC 2 cut(s) 109, 112
PvuII CAGCTG 1 cut(s) 110
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 2 cut(s) 108, 111
Sau3AI GATC 2 cut(s) 9, 20
SetI ASST 4 cut(s) 112, 183, 208, 250
SfaNI GCATC 1 cut(s) 214
SfcI CTRYAG 1 cut(s) 16
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 183
TfiI GAWTC 3 cut(s) 56, 74, 141
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 2 cut(s) 72, 139
TseI GCWGC 2 cut(s) 107, 110
TspDTI ATGAA 1 cut(s) 71
TspRI CASTG 2 cut(s) 72, 139
XmiI GTMKAC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.