Rmu_sc0001860.1_g000009

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001860.1
OG0021630
Physical Location & Seq
Forward (+)
46615 .. 49786
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001860.1_g000009.1.cds

Sequence Viewer

Length: 591 bp
atggatggtggtcacggtcttgtaggagatggaggtttgaggagtgaattgggcagtcttggaatggtttttagccaggaagtgataggaaatgaattatcttggctacgaagtgatcttggctgggaagtgatgccaattatgttctggacgttgcctatttatagaggagcattgagtctagaatcggcggcggaaccaccagaacaggttggaaaagctccgaataggccaccggaagctccgaatcggatgagtaacatgaacaaactggactttgctccattgggaacaactagctttgaatatcacaagtgggttcgtgatgtccgccagcatctcaaggcccaaggactcctagatacgattcttgagcctagccaagacattctaactattgagcaagctcaagctttggaagcaaatagagcagccttgcaggaaaataaggcaaaagccatcatcctaatgactcatcatatggataattcgcaccagtacgagtatatgaatgaagaagaccccagaaagctatgggtctcacttgaagaacgatttggcaacatccatgactccctgcttcctgactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

196

Amino Acids

22.1

Weight (kDa)

4.97

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 191, 194, 331
AfaI GTAC 1 cut(s) 500
AgsI TTSAA 2 cut(s) 305, 548
AjnI CCWGG 1 cut(s) 75
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 6 cut(s) 221, 242, 300, 407, 413, 532
AluI AGCT 6 cut(s) 221, 242, 300, 407, 413, 532
Alw26I GTCTC 1 cut(s) 544
AoxI GGCC 2 cut(s) 230, 345
ApeKI GCWGC 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 443
BccI CCATC 2 cut(s) 23, 467
BciT130I CCWGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 4 cut(s) 182, 297, 359, 378
BisI GCNGC 2 cut(s) 192, 432
BlsI GCNGC 2 cut(s) 193, 433
Bme1390I CCNGG 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 2 cut(s) 123, 346
BpiI GAAGAC 1 cut(s) 525
BpuEI CTTGAG 3 cut(s) 326, 392, 393
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 1 cut(s) 349
BsaWI WCCGGW 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 276, 496
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 349
BseGI GGATG 4 cut(s) 10, 258, 462, 564
BseNI ACTGG 2 cut(s) 276, 496
BseRI GAGGAG 2 cut(s) 55, 183
BseXI GCAGC 1 cut(s) 443
BseYI CCCAGC 1 cut(s) 123
BshFI GGCC 2 cut(s) 232, 347
BsiSI CCGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 544
BsnI GGCC 2 cut(s) 232, 347
Bso31I GGTCTC 1 cut(s) 544
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 3 cut(s) 191, 194, 331
BspANI GGCC 2 cut(s) 232, 347
BspLI GGNNCC 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 544
BsrI ACTGG 2 cut(s) 276, 496
BssECI CCNNGG 1 cut(s) 349
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 335, 405
BstF5I GGATG 4 cut(s) 10, 258, 462, 564
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 2 cut(s) 419, 428
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 443
BstV2I GAAGAC 1 cut(s) 525
BsuRI GGCC 2 cut(s) 232, 347
BtsCI GGATG 4 cut(s) 10, 258, 462, 564
Cac8I GCNNGC 2 cut(s) 335, 405
Cfr13I GGNCC 1 cut(s) 346
Csp6I GTAC 1 cut(s) 499
CviAII CATG 2 cut(s) 262, 569
CviQI GTAC 1 cut(s) 499
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
EciI GGCGGA 2 cut(s) 209, 320
Eco130I CCWWGG 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 2 cut(s) 265, 572
FaiI YATR 9 cut(s) 143, 165, 263, 480, 482, 507, 509, 535, 570
FatI CATG 2 cut(s) 261, 568
FauNDI CATATG 1 cut(s) 480
Fnu4HI GCNGC 2 cut(s) 192, 432
FokI GGATG 4 cut(s) 17, 265, 449, 551
Fsp4HI GCNGC 2 cut(s) 192, 432
FspBI CTAG 4 cut(s) 182, 297, 359, 378
GluI GCNGC 2 cut(s) 192, 432
GsaI CCCAGC 1 cut(s) 127
HaeIII GGCC 2 cut(s) 232, 347
HapII CCGG 1 cut(s) 236
Hin1II CATG 2 cut(s) 265, 572
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 7 cut(s) 178, 185, 247, 354, 367, 472, 572
HpaII CCGG 1 cut(s) 236
Hpy188I TCNGA 3 cut(s) 225, 246, 252
Hpy188III TCNNGA 5 cut(s) 148, 182, 323, 371, 584
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 1 cut(s) 439
HpyF10VI GCNNNNNNNGC 2 cut(s) 419, 428
HpySE526I ACGT 1 cut(s) 152
Hsp92II CATG 2 cut(s) 265, 572
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 4 cut(s) 170, 226, 247, 286
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 2 cut(s) 123, 346
MaeI CTAG 4 cut(s) 182, 297, 359, 378
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 2 cut(s) 11, 257
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 3 cut(s) 527, 530, 560
MluCI AATT 4 cut(s) 47, 95, 138, 487
MlyI GAGTC 4 cut(s) 187, 348, 466, 566
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 3 cut(s) 26, 33, 161
MslI CAYNNNNRTG 1 cut(s) 467
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 2 cut(s) 419, 428
NdeI CATATG 1 cut(s) 480
NdeII GATC 1 cut(s) 115
NlaIII CATG 2 cut(s) 265, 572
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 11
PfeI GAWTC 3 cut(s) 185, 247, 367
PkrI GCNGC 2 cut(s) 193, 433
PleI GAGTC 4 cut(s) 186, 348, 466, 566
PpsI GAGTC 4 cut(s) 186, 348, 466, 566
Psp6I CCWGG 1 cut(s) 75
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 346
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 1 cut(s) 467
SatI GCNGC 2 cut(s) 192, 432
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 4 cut(s) 187, 348, 466, 566
ScrFI CCNGG 1 cut(s) 77
SetI ASST 9 cut(s) 37, 155, 213, 223, 244, 302, 409, 415, 534
SfaNI GCATC 2 cut(s) 123, 346
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 3 cut(s) 341, 371, 408
SmoI CTYRAG 3 cut(s) 341, 371, 408
Sse9I AATT 4 cut(s) 47, 95, 138, 487
SsiI CCGC 3 cut(s) 191, 194, 331
SspMI CTAG 4 cut(s) 182, 297, 359, 378
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 1 cut(s) 155
TasI AATT 4 cut(s) 47, 95, 138, 487
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 3 cut(s) 185, 247, 367
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 4 cut(s) 108, 278, 524, 528
XbaI TCTAGA 1 cut(s) 181
XcmI CCANNNNNNNNNTGG 1 cut(s) 144
XspI CTAG 4 cut(s) 182, 297, 359, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.