Rmu_sc0002867.1_g000018

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002867.1
OG0021630
Physical Location & Seq
Reverse (-)
84039 .. 85326
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002867.1_g000018.1.cds

Sequence Viewer

Length: 765 bp
atgattcaagaaactaaaaaccttgaatccgaatacaaaaccttgaagccttttttgcctccacctcacaccttaaagaactcgattccaggagtccagaaccggcggcggtgccctaagaaccagccggaaacataccgaaccggccaccggaagctccatacaacccgcaacaaatatttcaccgaaaaaccggcagaaggaaaagagaaaaaacaaaaaaaaaaggaggaaggagaagctacagcccagaggcccaagccgagcccaggagcccagaagtccaagccgcagtcgctgacgtcatctcaggcacgtcagctacgccgcgtcatcaaccacgtcatcagacagtcagcagccaagtcagcggatctgccacgtcagcaactcggtcaaccctcctgtcaactactttactggatgagtaacctgaataaattggactttgctctattgagaaaaactggctctgaatatcacaggtgggttcgtgatatccgccagcatctcaaggccgatggaatgttggatacgattctcgagcctagccaggacgtgttaactgttaagcaagctaaagctttggaagcaaatagagcagccttagaggcaaataaggcgaaagccatcgtcctaatgactcttcatatggatgattcactccagtaccagtgtatgaatgaagaagaccccagaaggctgtgggtctcactcgaagatagatttggcaacgtccgtgactccctgcttcctgacctataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

254

Amino Acids

29.28

Weight (kDa)

9.87

Isoelectric Point (pI)

59.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 1 cut(s) 330
AciI CCGC 7 cut(s) 106, 109, 169, 290, 328, 371, 502
AclWI GGATC 1 cut(s) 381
AcoI YGGCCR 1 cut(s) 145
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 1 cut(s) 671
AfiI CCNNNNNNNGG 3 cut(s) 150, 200, 269
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 3 cut(s) 8, 26, 46
AjiI CACGTC 4 cut(s) 317, 343, 383, 559
AjnI CCWGG 3 cut(s) 88, 268, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 711, 743
AluBI AGCT 5 cut(s) 157, 242, 322, 578, 584
AluI AGCT 5 cut(s) 157, 242, 322, 578, 584
Alw26I GTCTC 1 cut(s) 715
AlwI GGATC 1 cut(s) 381
AlwNI CAGNNNCTG 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 542
AoxI GGCC 3 cut(s) 145, 254, 516
ApeKI GCWGC 2 cut(s) 359, 602
AspS9I GGNCC 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 116
BaeI ACNNNNGTAYC 2 cut(s) 653, 686
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 2 cut(s) 269, 277
BbsI GAAGAC 1 cut(s) 696
BbvI GCAGC 2 cut(s) 371, 614
BccI CCATC 2 cut(s) 515, 638
BciT130I CCWGG 3 cut(s) 90, 270, 554
BciVI GTATCC 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 715
BfaI CTAG 1 cut(s) 549
BfmI CTRYAG 1 cut(s) 243
BfuI GTATCC 1 cut(s) 526
BglI GCCNNNNNGGC 1 cut(s) 611
BisI GCNGC 5 cut(s) 107, 290, 328, 360, 603
BlsI GCNGC 5 cut(s) 108, 291, 329, 361, 604
Bme1390I CCNGG 3 cut(s) 90, 270, 554
BmeT110I CYCGRG 1 cut(s) 542
BmgBI CACGTC 4 cut(s) 317, 343, 383, 559
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 2 cut(s) 113, 274
BmrFI CCNGG 3 cut(s) 90, 270, 554
BmsI GCATC 1 cut(s) 517
BpiI GAAGAC 1 cut(s) 696
BpmI CTGGAG 1 cut(s) 650
BpuEI CTTGAG 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 715
BsaJI CCNNGG 1 cut(s) 268
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 200, 269
Bse118I RCCGGY 3 cut(s) 102, 143, 193
Bse1I ACTGG 4 cut(s) 425, 472, 667, 673
BseBI CCWGG 3 cut(s) 90, 270, 554
BseDI CCNNGG 1 cut(s) 268
BseGI GGATG 2 cut(s) 429, 661
BseLI CCNNNNNNNGG 3 cut(s) 150, 200, 269
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 4 cut(s) 425, 472, 667, 673
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 2 cut(s) 371, 614
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 147, 256, 518
BshNI GGYRCC 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 5 cut(s) 103, 128, 144, 151, 194
BslI CCNNNNNNNGG 3 cut(s) 150, 200, 269
BsmAI GTCTC 1 cut(s) 715
BsnI GGCC 3 cut(s) 147, 256, 518
Bso31I GGTCTC 1 cut(s) 715
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 3 cut(s) 116, 269, 277
Bsp143I GATC 1 cut(s) 373
BspACI CCGC 7 cut(s) 106, 109, 169, 290, 328, 371, 502
BspANI GGCC 3 cut(s) 147, 256, 518
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 330
BspLI GGNNCC 2 cut(s) 113, 274
BspPI GGATC 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 715
BsrFI RCCGGY 3 cut(s) 102, 143, 193
BsrI ACTGG 4 cut(s) 425, 472, 667, 673
BssAI RCCGGY 3 cut(s) 102, 143, 193
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 1 cut(s) 373
BssNI GRCGYC 1 cut(s) 302
Bst2UI CCWGG 3 cut(s) 90, 270, 554
Bst4CI ACNGT 2 cut(s) 354, 568
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 1 cut(s) 302
BstC8I GCNNGC 2 cut(s) 506, 576
BstDEI CTNAG 3 cut(s) 117, 309, 607
BstF5I GGATG 2 cut(s) 429, 661
BstFNI CGCG 1 cut(s) 330
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 1 cut(s) 715
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 8 cut(s) 55, 295, 368, 385, 590, 599, 611, 620
BstNI CCWGG 3 cut(s) 90, 270, 554
BstSCI CCNGG 3 cut(s) 88, 268, 552
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 116
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 371, 614
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 3 cut(s) 147, 256, 518
BtrI CACGTC 4 cut(s) 317, 343, 383, 559
BtsCI GGATG 2 cut(s) 429, 661
BtsIMutI CAGTG 1 cut(s) 680
Cac8I GCNNGC 2 cut(s) 506, 576
CaiI CAGNNNCTG 1 cut(s) 298
Cfr10I RCCGGY 3 cut(s) 102, 143, 193
Cfr13I GGNCC 1 cut(s) 255
CseI GACGC 1 cut(s) 319
Csp6I GTAC 1 cut(s) 670
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 3 cut(s) 117, 309, 607
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
EciI GGCGGA 1 cut(s) 491
Eco24I GRGCYC 2 cut(s) 269, 277
Eco31I GGTCTC 1 cut(s) 715
Eco32I GATATC 1 cut(s) 499
Eco88I CYCGRG 1 cut(s) 542
EcoRII CCWGG 3 cut(s) 88, 268, 552
EcoRV GATATC 1 cut(s) 499
EcoT38I GRGCYC 2 cut(s) 269, 277
FaiI YATR 6 cut(s) 136, 162, 651, 653, 680, 763
FauI CCCGC 1 cut(s) 176
FauNDI CATATG 1 cut(s) 651
Fnu4HI GCNGC 5 cut(s) 107, 290, 328, 360, 603
FokI GGATG 2 cut(s) 436, 668
FriOI GRGCYC 2 cut(s) 269, 277
Fsp4HI GCNGC 5 cut(s) 107, 290, 328, 360, 603
FspBI CTAG 1 cut(s) 549
GluI GCNGC 5 cut(s) 107, 290, 328, 360, 603
GsuI CTGGAG 1 cut(s) 650
HaeIII GGCC 3 cut(s) 147, 256, 518
HapII CCGG 5 cut(s) 103, 128, 144, 151, 194
HgaI GACGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 302
HincII GTYRAC 3 cut(s) 398, 410, 564
HindII GTYRAC 3 cut(s) 398, 410, 564
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 8 cut(s) 4, 26, 85, 93, 538, 643, 659, 743
HpaI GTTAAC 1 cut(s) 564
HpaII CCGG 5 cut(s) 103, 128, 144, 151, 194
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 3 cut(s) 398, 410, 564
Hpy188I TCNGA 3 cut(s) 31, 350, 475
Hpy188III TCNNGA 5 cut(s) 8, 97, 494, 542, 755
Hpy8I GTNNAC 3 cut(s) 398, 410, 564
HpyAV CCTTC 3 cut(s) 194, 227, 693
HpyCH4III ACNGT 2 cut(s) 354, 568
HpyCH4IV ACGT 6 cut(s) 302, 316, 342, 382, 558, 735
HpyF10VI GCNNNNNNNGC 8 cut(s) 55, 295, 368, 385, 590, 599, 611, 620
HpyF3I CTNAG 3 cut(s) 117, 309, 607
HpySE526I ACGT 6 cut(s) 302, 316, 342, 382, 558, 735
Hsp92I GRCGYC 1 cut(s) 302
KspAI GTTAAC 1 cut(s) 564
Kzo9I GATC 1 cut(s) 373
LmnI GCTCC 2 cut(s) 162, 272
Lsp1109I GCAGC 2 cut(s) 371, 614
LweI GCATC 1 cut(s) 517
MaeI CTAG 1 cut(s) 549
MaeII ACGT 6 cut(s) 302, 316, 342, 382, 558, 735
MaeIII GTNAC 2 cut(s) 428, 740
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 4 cut(s) 638, 698, 701, 731
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 3 cut(s) 116, 269, 277
MluCI AATT 1 cut(s) 440
MlyI GAGTC 3 cut(s) 102, 637, 737
MmeI TCCRAC 1 cut(s) 510
MnlI CCTC 6 cut(s) 69, 75, 223, 246, 412, 604
MseI TTAA 3 cut(s) 74, 563, 570
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 5 cut(s) 103, 128, 144, 151, 194
MspR9I CCNGG 3 cut(s) 90, 270, 554
MvaI CCWGG 3 cut(s) 90, 270, 554
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 8 cut(s) 55, 295, 368, 385, 590, 599, 611, 620
NdeI CATATG 1 cut(s) 651
NdeII GATC 1 cut(s) 373
NlaIV GGNNCC 2 cut(s) 113, 274
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 740
PaeR7I CTCGAG 1 cut(s) 542
PfeI GAWTC 5 cut(s) 4, 26, 85, 538, 659
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 5 cut(s) 108, 291, 329, 361, 604
PleI GAGTC 3 cut(s) 101, 637, 737
PpsI GAGTC 3 cut(s) 101, 637, 737
Psp6I CCWGG 3 cut(s) 88, 268, 552
PspGI CCWGG 3 cut(s) 88, 268, 552
PspN4I GGNNCC 2 cut(s) 113, 274
PspPI GGNCC 1 cut(s) 255
PstNI CAGNNNCTG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 373
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
RseI CAYNNNNRTG 1 cut(s) 654
SaqAI TTAA 3 cut(s) 74, 563, 570
SatI GCNGC 5 cut(s) 107, 290, 328, 360, 603
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 1 cut(s) 255
SchI GAGTC 3 cut(s) 102, 637, 737
ScrFI CCNGG 3 cut(s) 90, 270, 554
SduI GDGCHC 3 cut(s) 116, 269, 277
SfaNI GCATC 1 cut(s) 517
SfcI CTRYAG 1 cut(s) 243
Sfr274I CTCGAG 1 cut(s) 542
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 512, 542
SmoI CTYRAG 2 cut(s) 512, 542
Sse9I AATT 1 cut(s) 440
SsiI CCGC 7 cut(s) 106, 109, 169, 290, 328, 371, 502
SspI AATATT 1 cut(s) 179
SspMI CTAG 1 cut(s) 549
StyD4I CCNGG 3 cut(s) 88, 268, 552
TaaI ACNGT 2 cut(s) 354, 568
TaiI ACGT 6 cut(s) 305, 319, 345, 385, 561, 738
TaqI TCGA 3 cut(s) 83, 543, 717
TaqII GACCGA 1 cut(s) 383
TasI AATT 1 cut(s) 440
TauI GCSGC 3 cut(s) 109, 292, 330
TfiI GAWTC 5 cut(s) 4, 26, 85, 538, 659
Tru1I TTAA 3 cut(s) 74, 563, 570
Tru9I TTAA 3 cut(s) 74, 563, 570
TscAI CASTG 1 cut(s) 680
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 2 cut(s) 359, 602
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 3 cut(s) 638, 695, 699
TspGWI ACGGA 1 cut(s) 728
TspRI CASTG 1 cut(s) 680
XhoI CTCGAG 1 cut(s) 542
XspI CTAG 1 cut(s) 549
ZraI GACGTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.