Rmu_sc0001992.1_g000036

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001992.1
OG0022147
Physical Location & Seq
Forward (+)
183180 .. 185025
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001992.1_g000036.1.cds

Sequence Viewer

Length: 633 bp
atgctgcatgtggatgtggtgtcgctgctctacacccagaaggcccaggcggtgttcgatgaaatagccaacaggggtttggacccaactgtagttagtttcaataccttgattaatggctattataaatctggcaatctagagcagggttttaagttgaaggcagatatggaggggagaccaatgtgtcctgatttgattacttacaacgttttaataaatggcttgtgtaaggaaagcaggttggatgatgcaaatgggttgtttgatgaatccaatagagagaagaagtggatggttggaattgcagaggttggcggcctggcacttttctggtcagaagatgttgacctacacgttcgtaccttttctgcgcatcacatcgacgcagaagttagggcaggacctgactctcttctgtggataataatcggcgatttcaatgagatattgaataatagtgagaagctgggaaggcctttgcagcctgaaaggcaaatgagaggcttccgtgaagctttgggttatggaaacctagttgatctagggtttcaaggcacaaagatgacatggtggagtgcagaaaacccaattatgtttggacagagcggttgggacttctacatggagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

210

Amino Acids

23.77

Weight (kDa)

4.81

Isoelectric Point (pI)

26.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022147)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g30170
rosa_multiflora Rmu_sc0001992.1_g000036
rosa_roxburghii Rroxscaffold_4G00332140
rosa_rugosa Rorug03G0282000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 126
AasI GACNNNNNNGTC 1 cut(s) 186
Acc16I TGCGCA 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 231
AccBSI CCGCTC 1 cut(s) 609
AciI CCGC 3 cut(s) 50, 318, 609
AclI AACGTT 1 cut(s) 210
AfaI GTAC 1 cut(s) 364
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 5 cut(s) 103, 160, 442, 454, 554
AjnI CCWGG 2 cut(s) 45, 321
AluBI AGCT 2 cut(s) 469, 518
AluI AGCT 2 cut(s) 469, 518
Alw26I GTCTC 1 cut(s) 172
AlwNI CAGNNNCTG 1 cut(s) 407
AoxI GGCC 3 cut(s) 42, 319, 476
ApeKI GCWGC 3 cut(s) 4, 25, 484
AseI ATTAAT 1 cut(s) 114
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 3 cut(s) 43, 82, 404
AvaII GGWCC 2 cut(s) 82, 404
BaeI ACNNNNGTAYC 2 cut(s) 346, 379
BbvI GCAGC 2 cut(s) 12, 496
BccI CCATC 1 cut(s) 289
BciT130I CCWGG 2 cut(s) 47, 323
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 3 cut(s) 140, 536, 545
BfmI CTRYAG 1 cut(s) 90
BfuAI ACCTGC 1 cut(s) 231
BglI GCCNNNNNGGC 1 cut(s) 493
BisI GCNGC 4 cut(s) 5, 26, 319, 485
BlsI GCNGC 4 cut(s) 6, 27, 320, 486
Bme1390I CCNGG 2 cut(s) 47, 323
Bme18I GGWCC 2 cut(s) 82, 404
BmgT120I GGNCC 3 cut(s) 43, 82, 404
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 47, 323
BmsI GCATC 2 cut(s) 241, 385
BsaBI GATNNNNATC 1 cut(s) 428
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 428
BseBI CCWGG 2 cut(s) 47, 323
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 3 cut(s) 19, 253, 300
BseJI GATNNNNATC 1 cut(s) 428
BseXI GCAGC 2 cut(s) 12, 496
BseYI CCCAGC 1 cut(s) 469
BsgI GTGCAG 1 cut(s) 600
BshFI GGCC 3 cut(s) 44, 321, 478
BslFI GGGAC 1 cut(s) 629
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 3 cut(s) 44, 321, 478
Bso31I GGTCTC 1 cut(s) 172
Bsp143I GATC 1 cut(s) 541
BspACI CCGC 3 cut(s) 50, 318, 609
BspANI GGCC 3 cut(s) 44, 321, 478
BspLI GGNNCC 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 231
BspTNI GGTCTC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 609
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 1 cut(s) 541
Bst2UI CCWGG 2 cut(s) 47, 323
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 1 cut(s) 420
BstF5I GGATG 3 cut(s) 19, 253, 300
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 1 cut(s) 544
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 1 cut(s) 541
BstMWI GCNNNNNNNGC 3 cut(s) 475, 484, 493
BstNI CCWGG 2 cut(s) 47, 323
BstNSI RCATGY 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 45, 321
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 12, 496
BsuRI GGCC 3 cut(s) 44, 321, 478
BtsCI GGATG 3 cut(s) 19, 253, 300
BveI ACCTGC 1 cut(s) 231
CaiI CAGNNNCTG 1 cut(s) 407
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 43, 82, 404
CseI GACGC 1 cut(s) 395
Csp6I GTAC 1 cut(s) 363
CviAII CATG 3 cut(s) 8, 570, 625
CviQI GTAC 1 cut(s) 363
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
DrdI GACNNNNNNGTC 1 cut(s) 186
DseDI GACNNNNNNGTC 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco147I AGGCCT 1 cut(s) 478
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 82, 404
EcoO109I RGGNCCY 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 45, 321
FaeI CATG 3 cut(s) 11, 573, 628
FaiI YATR 7 cut(s) 9, 126, 170, 528, 571, 596, 626
FaqI GGGAC 1 cut(s) 629
FatI CATG 3 cut(s) 7, 569, 624
Fnu4HI GCNGC 4 cut(s) 5, 26, 319, 485
FokI GGATG 3 cut(s) 26, 260, 307
Fsp4HI GCNGC 4 cut(s) 5, 26, 319, 485
FspBI CTAG 3 cut(s) 140, 536, 545
FspI TGCGCA 1 cut(s) 375
GlaI GCGC 1 cut(s) 375
GluI GCNGC 4 cut(s) 5, 26, 319, 485
GsaI CCCAGC 1 cut(s) 473
HaeIII GGCC 3 cut(s) 44, 321, 478
HgaI GACGC 1 cut(s) 395
HhaI GCGC 1 cut(s) 376
Hin1II CATG 3 cut(s) 11, 573, 628
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 2 cut(s) 272, 410
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 2 cut(s) 140, 191
Hpy8I GTNNAC 1 cut(s) 349
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 3 cut(s) 34, 154, 468
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 2 cut(s) 210, 357
HpyCH4V TGCA 5 cut(s) 7, 254, 308, 484, 581
HpyF10VI GCNNNNNNNGC 3 cut(s) 475, 484, 493
HpySE526I ACGT 2 cut(s) 210, 357
Hsp92II CATG 3 cut(s) 11, 573, 628
HspAI GCGC 1 cut(s) 374
Kzo9I GATC 1 cut(s) 541
Lsp1109I GCAGC 2 cut(s) 12, 496
LweI GCATC 2 cut(s) 241, 385
MaeI CTAG 3 cut(s) 140, 536, 545
MaeII ACGT 2 cut(s) 210, 357
MalI GATC 1 cut(s) 543
MbiI CCGCTC 1 cut(s) 609
MboI GATC 1 cut(s) 541
MboII GAAGA 3 cut(s) 298, 353, 407
MluCI AATT 2 cut(s) 303, 591
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 225, 280
MnlI CCTC 3 cut(s) 166, 304, 497
MseI TTAA 3 cut(s) 114, 153, 215
MslI CAYNNNNRTG 2 cut(s) 12, 563
MspR9I CCNGG 2 cut(s) 47, 323
MvaI CCWGG 2 cut(s) 47, 323
MwoI GCNNNNNNNGC 3 cut(s) 475, 484, 493
NdeII GATC 1 cut(s) 541
NlaIII CATG 3 cut(s) 11, 573, 628
NlaIV GGNNCC 1 cut(s) 84
NsbI TGCGCA 1 cut(s) 375
NspI RCATGY 1 cut(s) 11
PceI AGGCCT 1 cut(s) 478
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 6, 27, 320, 486
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PpuMI RGGWCCY 1 cut(s) 404
PshBI ATTAAT 1 cut(s) 114
PsiI TTATAA 1 cut(s) 126
Psp1406I AACGTT 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 45, 321
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 2 cut(s) 45, 321
PspN4I GGNNCC 1 cut(s) 84
PspPI GGNCC 3 cut(s) 43, 82, 404
PspPPI RGGWCCY 1 cut(s) 404
PstNI CAGNNNCTG 1 cut(s) 407
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
RseI CAYNNNNRTG 2 cut(s) 12, 563
SaqAI TTAA 3 cut(s) 114, 153, 215
SatI GCNGC 4 cut(s) 5, 26, 319, 485
Sau3AI GATC 1 cut(s) 541
Sau96I GGNCC 3 cut(s) 43, 82, 404
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 2 cut(s) 47, 323
SfaNI GCATC 2 cut(s) 241, 385
SfcI CTRYAG 1 cut(s) 90
SinI GGWCC 2 cut(s) 82, 404
SmiMI CAYNNNNRTG 2 cut(s) 12, 563
Sse9I AATT 2 cut(s) 303, 591
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 3 cut(s) 50, 318, 609
SspMI CTAG 3 cut(s) 140, 536, 545
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 2 cut(s) 45, 321
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 2 cut(s) 213, 360
TaqI TCGA 2 cut(s) 57, 384
TasI AATT 2 cut(s) 303, 591
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 3 cut(s) 114, 153, 215
Tru9I TTAA 3 cut(s) 114, 153, 215
TseI GCWGC 3 cut(s) 4, 25, 484
TspDTI ATGAA 2 cut(s) 75, 285
TspGWI ACGGA 1 cut(s) 500
VpaK11BI GGWCC 2 cut(s) 82, 404
VspI ATTAAT 1 cut(s) 114
XbaI TCTAGA 1 cut(s) 139
XceI RCATGY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XspI CTAG 3 cut(s) 140, 536, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.