Rmu_sc0002087.1_g000013

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002087.1
OG0021618
Physical Location & Seq
Reverse (-)
58444 .. 59911
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
atgcaacaggtggcggcggctcggggctggccggaggcgtcccagacctcttctgggcggcgttcgaggttggaggaggtcgggcttgccggtttctgggcagccacctcacctgcagttgcaggttctgtgcagcaccgcagaaccgtcgccggcgactccaccggaccgcagacccctccgcacgacccccagggtcccttcggcaagccgggccgcgctgtcgccgctcgatcgaggctggaggagcgatgtccgcactttttctgggttgcggacgtccgctctcgaacggctccgatatatatatactatactgatccatctggcacattttggcaatgcaatgcaaaagctataggttcgggttcagaaggtgcagacagctctctgcaagagcaatacagcaaggacttgactcttaaagaagctgagacaattgctctttccattcttaagcaagtcatggaggaaaaggtgactcccaataatgttgatattgcaaaggtttcacccacatatcatctatataccccttctgaggtggaggaggtcatcagtcgcctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019773 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome Proteasome_A_N

Protein Analysis

189

Amino Acids

20.59

Weight (kDa)

6.43

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 102 - 170 2e-15 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021618)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002087.1_g000013 Rmu_sc0009453.1_g000001 Rmu_ssc0000252.1_g000014
rosa_roxburghii Rroxscaffold_4G00298070
rosa_rugosa Rorug06G0511100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 121
AatII GACGTC 1 cut(s) 282
Acc36I ACCTGC 2 cut(s) 113, 121
AccBSI CCGCTC 2 cut(s) 230, 285
AccII CGCG 1 cut(s) 219
AclWI GGATC 1 cut(s) 314
AcoI YGGCCR 1 cut(s) 29
AcyI GRCGYC 2 cut(s) 38, 279
AfiI CCNNNNNNNGG 3 cut(s) 54, 96, 541
AflII CTTAAG 1 cut(s) 455
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 3 cut(s) 356, 387, 431
AluI AGCT 3 cut(s) 356, 387, 431
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 21
AoxI GGCC 2 cut(s) 29, 214
ApeKI GCWGC 2 cut(s) 101, 133
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 167, 197, 214
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 3 cut(s) 102, 490, 504
AvaI CYCGRG 1 cut(s) 21
AvaII GGWCC 2 cut(s) 167, 197
BbvI GCAGC 2 cut(s) 113, 145
BccI CCATC 1 cut(s) 331
BceAI ACGGC 1 cut(s) 309
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 194
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 428
BfmI CTRYAG 2 cut(s) 114, 357
BfrI CTTAAG 1 cut(s) 455
BfuAI ACCTGC 2 cut(s) 113, 121
BisI GCNGC 7 cut(s) 15, 18, 59, 102, 134, 217, 228
BlsI GCNGC 7 cut(s) 16, 19, 60, 103, 135, 218, 229
Bme1390I CCNGG 2 cut(s) 194, 213
Bme18I GGWCC 2 cut(s) 167, 197
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 167, 197, 214
BmiI GGNNCC 3 cut(s) 198, 199, 297
BmrFI CCNGG 2 cut(s) 194, 213
BpmI CTGGAG 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 213
BsaHI GRCGYC 2 cut(s) 38, 279
BsaJI CCNNGG 2 cut(s) 192, 193
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 96, 541
Bse118I RCCGGY 2 cut(s) 89, 152
Bse3DI GCAATG 2 cut(s) 347, 352
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 192, 193
BseLI CCNNNNNNNGG 3 cut(s) 54, 96, 541
BseMI GCAATG 2 cut(s) 347, 352
BseMII CTCAG 2 cut(s) 423, 531
BseRI GAGGAG 3 cut(s) 89, 260, 563
BseXI GCAGC 2 cut(s) 113, 145
BsgI GTGCAG 2 cut(s) 152, 399
Bsh1236I CGCG 1 cut(s) 219
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 31, 216
BsiEI CGRYCG 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 21
BsiSI CCGG 5 cut(s) 32, 90, 153, 165, 212
BslFI GGGAC 2 cut(s) 25, 183
BslI CCNNNNNNNGG 3 cut(s) 54, 96, 541
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 2 cut(s) 25, 183
BsnI GGCC 2 cut(s) 31, 216
BsoBI CYCGRG 1 cut(s) 21
Bsp143I GATC 2 cut(s) 233, 319
BspANI GGCC 2 cut(s) 31, 216
BspCNI CTCAG 2 cut(s) 424, 532
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 198, 199, 297
BspMAI CTGCAG 1 cut(s) 118
BspMI ACCTGC 2 cut(s) 113, 121
BspPI GGATC 1 cut(s) 314
BspTI CTTAAG 1 cut(s) 455
BsrBI CCGCTC 2 cut(s) 230, 285
BsrDI GCAATG 2 cut(s) 347, 352
BsrFI RCCGGY 2 cut(s) 89, 152
BssAI RCCGGY 2 cut(s) 89, 152
BssECI CCNNGG 2 cut(s) 192, 193
BssMI GATC 2 cut(s) 233, 319
BssNI GRCGYC 2 cut(s) 38, 279
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 55
BstACI GRCGYC 2 cut(s) 38, 279
BstAFI CTTAAG 1 cut(s) 455
BstC8I GCNNGC 4 cut(s) 29, 87, 154, 209
BstDEI CTNAG 2 cut(s) 432, 540
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 2 cut(s) 236, 322
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 2 cut(s) 233, 319
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 4 cut(s) 213, 227, 247, 256
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 2 cut(s) 192, 211
BstSFI CTRYAG 2 cut(s) 114, 357
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 113, 145
BsuRI GGCC 2 cut(s) 31, 216
BtgZI GCGATG 1 cut(s) 265
BveI ACCTGC 2 cut(s) 113, 121
Cac8I GCNNGC 4 cut(s) 29, 87, 154, 209
CaiI CAGNNNCTG 1 cut(s) 128
CfoI GCGC 1 cut(s) 221
Cfr10I RCCGGY 2 cut(s) 89, 152
Cfr13I GGNCC 3 cut(s) 167, 197, 214
CpoI CGGWCCG 1 cut(s) 167
CseI GACGC 1 cut(s) 27
CspI CGGWCCG 1 cut(s) 167
CviAII CATG 1 cut(s) 466
DdeI CTNAG 2 cut(s) 432, 540
DpnI GATC 2 cut(s) 235, 321
DpnII GATC 2 cut(s) 233, 319
EaeI YGGCCR 1 cut(s) 29
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 167, 197
Eco88I CYCGRG 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 192
FaeI CATG 1 cut(s) 469
FaqI GGGAC 2 cut(s) 25, 183
FatI CATG 1 cut(s) 465
Fnu4HI GCNGC 7 cut(s) 15, 18, 59, 102, 134, 217, 228
Fsp4HI GCNGC 7 cut(s) 15, 18, 59, 102, 134, 217, 228
GlaI GCGC 1 cut(s) 220
GluI GCNGC 7 cut(s) 15, 18, 59, 102, 134, 217, 228
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 2 cut(s) 31, 216
HapII CCGG 5 cut(s) 32, 90, 153, 165, 212
HgaI GACGC 1 cut(s) 27
HhaI GCGC 1 cut(s) 221
Hin1I GRCGYC 2 cut(s) 38, 279
Hin1II CATG 1 cut(s) 469
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HinfI GANTC 3 cut(s) 158, 418, 481
HpaII CCGG 5 cut(s) 32, 90, 153, 165, 212
HphI GGTGA 3 cut(s) 102, 490, 504
Hpy188I TCNGA 3 cut(s) 300, 373, 541
Hpy188III TCNNGA 1 cut(s) 288
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 3 cut(s) 211, 368, 546
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 9 cut(s) 4, 116, 122, 133, 345, 350, 380, 394, 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 213, 227, 247, 256
HpyF3I CTNAG 2 cut(s) 432, 540
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 2 cut(s) 38, 279
Hsp92II CATG 1 cut(s) 469
HspAI GCGC 1 cut(s) 219
KflI GGGWCCC 1 cut(s) 197
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 233, 319
LmnI GCTCC 2 cut(s) 247, 301
Lsp1109I GCAGC 2 cut(s) 113, 145
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 478
MalI GATC 2 cut(s) 235, 321
MbiI CCGCTC 2 cut(s) 230, 285
MboI GATC 2 cut(s) 233, 319
MboII GAAGA 1 cut(s) 42
MfeI CAATTG 1 cut(s) 438
MluCI AATT 1 cut(s) 438
MlyI GAGTC 3 cut(s) 152, 412, 475
MmeI TCCRAC 1 cut(s) 51
MreI CGCCGGCG 1 cut(s) 152
MroNI GCCGGC 1 cut(s) 152
MseI TTAA 2 cut(s) 423, 456
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 5 cut(s) 32, 90, 153, 165, 212
MspR9I CCNGG 2 cut(s) 194, 213
MunI CAATTG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 4 cut(s) 213, 227, 247, 256
NaeI GCCGGC 1 cut(s) 154
NciI CCSGG 1 cut(s) 213
NdeII GATC 2 cut(s) 233, 319
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 3 cut(s) 198, 199, 297
NmuCI GTSAC 1 cut(s) 478
PaqCI CACCTGC 1 cut(s) 121
PasI CCCWGGG 1 cut(s) 193
PdiI GCCGGC 1 cut(s) 154
PkrI GCNGC 7 cut(s) 16, 19, 60, 103, 135, 218, 229
Ple19I CGATCG 1 cut(s) 236
PleI GAGTC 3 cut(s) 152, 412, 475
PpsI GAGTC 3 cut(s) 152, 412, 475
PpuMI RGGWCCY 1 cut(s) 197
Psp5II RGGWCCY 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 3 cut(s) 198, 199, 297
PspPI GGNCC 3 cut(s) 167, 197, 214
PspPPI RGGWCCY 1 cut(s) 197
PstI CTGCAG 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 128
PvuI CGATCG 1 cut(s) 236
Rsr2I CGGWCCG 1 cut(s) 167
RsrII CGGWCCG 1 cut(s) 167
SaqAI TTAA 2 cut(s) 423, 456
SatI GCNGC 7 cut(s) 15, 18, 59, 102, 134, 217, 228
Sau3AI GATC 2 cut(s) 233, 319
Sau96I GGNCC 3 cut(s) 167, 197, 214
SchI GAGTC 3 cut(s) 152, 412, 475
ScrFI CCNGG 2 cut(s) 194, 213
SfcI CTRYAG 2 cut(s) 114, 357
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 2 cut(s) 167, 197
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 1 cut(s) 438
StyD4I CCNGG 2 cut(s) 192, 211
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 282
TaqI TCGA 4 cut(s) 65, 232, 236, 289
TasI AATT 1 cut(s) 438
TauI GCSGC 5 cut(s) 17, 20, 61, 219, 230
Tru1I TTAA 2 cut(s) 423, 456
Tru9I TTAA 2 cut(s) 423, 456
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 2 cut(s) 101, 133
Tsp45I GTSAC 1 cut(s) 478
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 167, 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.