Rmu_sc0002194.1_g000018

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002194.1
OG0021650
Physical Location & Seq
Reverse (-)
69757 .. 72824
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002194.1_g000018.1.cds

Sequence Viewer

Length: 1557 bp
atgtgtaccatccgtcaagcttggagcaatagtcagctccggttttcggccacattgctgaatctagtggagataaagattgaggtggataatgttacaacaaagaaattgccagataagctctgtgaacttattcagctaaagaagttctgttttaccagctttcataagctttctaccttgcctgaagagattggaaatctgatcaatttagaagtgttgaagctaaagtcctgcacagacttgccaaagttgccagactctgttacaaagctcaacaagttacactctcttgacatatctgagtgcttcagcattaatgagttgcccgaagacatcggagcattgtcaagcttaaaagagctcaacatgagccactgctcaaaattgcaccagttgcccctatcagttctgttacttgacaagttagagggagtgacatgtgatgaagagacaaagaagttatgggaaccctatttatcccatctgggaaacctacgaataaaggtggatctggccaaagaaaaccccttccctagtcaatctaatcttatagtcataaggatgcaggagagagaggtaatgcgcaacatgatgaaatcacatttcagcacagcagattttaaggaagatataagggcagtcatcaggaaggaatttgctgattttatagactgttgtggctacaagaaacaaaaaaaacaggaagtcgagggaacgtctaagatagaactggaaggcacaaacggttcggatccaacacgactttcagcatggatggatagtcaaattcaggctagggaatggacactgaggaagtttaatgcatggattgataacccaaacgcaggactgcagaacttggaaatatttcaagcttcaaaggaatggtttgaggacttgatgacaacaggcatgtggttgactgagtcgcacgtagatgttttcctgcattttgttcgcatgaatacacgtcatcaaccccagcctttctctcagaattgcaccatcttggatacttctttttggaatttcctgaatgatcaatgggaggctttcaagtgtaatcccgattcctatatatgggattcagatgtcatggactatgcactaggaaagtcacttacatacagtcgaccatggactgaagttgactctttgtaccttcctgtaaatgtcaaaaaccattggatagctgtatgtgtggaactaatgaaaagaaagttgaccatatacgactcagtgtgctccctgaatcaagattattttgacatattaaagccaatggcaaggatgttgccatcgttgctgatccagtcaggcttttatgcacagaggactgagctgtcccctctcagtaaaccatttgaagtgatgtgtttagcagctgacaacattccacaactgcctgacagaggagattgcggggttttcatgcttaaatatgtggagtatctaagcttgggaaaacctctgaatttcacacaagcagacattccactatttaggaagagaatggccttggacatgtttaccattggtgatgattcaggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

518

Amino Acids

59.79

Weight (kDa)

5.62

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021650)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002194.1_g000018
rosa_roxburghii Rroxscaffold_1G00027280
rosa_samantha Rh5BG360600 Rh5DG375400
rosa_wichuraiana Rw5G032970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1345
Acc16I TGCGCA 1 cut(s) 587
AccI GTMKAC 1 cut(s) 1135
AciI CCGC 1 cut(s) 1425
AclWI GGATC 4 cut(s) 519, 749, 762, 1306
AcoI YGGCCR 2 cut(s) 48, 516
AcsI RAATTY 4 cut(s) 656, 789, 1030, 1477
AcuI CTGAAG 3 cut(s) 207, 295, 1167
AfaI GTAC 2 cut(s) 7, 1163
AfiI CCNNNNNNNGG 4 cut(s) 46, 848, 1083, 1415
AflIII ACRYGT 3 cut(s) 440, 971, 1527
AgsI TTSAA 5 cut(s) 223, 875, 882, 1060, 1370
AjiI CACGTC 1 cut(s) 974
AjuI GAANNNNNNNTTGG 2 cut(s) 751, 783
Alw21I GWGCWC 2 cut(s) 366, 1250
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 4 cut(s) 519, 749, 762, 1306
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 3 cut(s) 48, 516, 1518
ApeKI GCWGC 1 cut(s) 1385
ApoI RAATTY 4 cut(s) 656, 789, 1030, 1477
AseI ATTAAT 1 cut(s) 318
Asp700I GAANNNNTTC 2 cut(s) 132, 870
AspLEI GCGC 1 cut(s) 588
AsuHPI GGTGA 1 cut(s) 1553
BalI TGGCCA 1 cut(s) 518
BamHI GGATCC 1 cut(s) 754
BanII GRGCYC 1 cut(s) 366
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 2 cut(s) 366, 1250
BbvI GCAGC 1 cut(s) 1397
BccI CCATC 5 cut(s) 17, 492, 772, 1016, 1309
BcgI CGANNNNNNTGC 2 cut(s) 941, 975
BciVI GTATCC 1 cut(s) 1009
BclI TGATCA 2 cut(s) 204, 1042
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 4 cut(s) 65, 537, 798, 1112
BfmI CTRYAG 1 cut(s) 854
BfuI GTATCC 1 cut(s) 1009
BisI GCNGC 1 cut(s) 1386
BlsI GCNGC 1 cut(s) 1387
BmgBI CACGTC 1 cut(s) 974
BmiI GGNNCC 2 cut(s) 471, 756
BmsI GCATC 1 cut(s) 555
BpiI GAAGAC 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 937
BsaJI CCNNGG 2 cut(s) 1139, 1521
BsaWI WCCGGW 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 848, 1083, 1415
Bse1I ACTGG 3 cut(s) 394, 738, 1315
Bse3DI GCAATG 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 1139, 1521
BseGI GGATG 4 cut(s) 9, 570, 783, 1299
BseLI CCNNNNNNNGG 4 cut(s) 46, 848, 1083, 1415
BseMI GCAATG 1 cut(s) 53
BseMII CTCAG 7 cut(s) 294, 803, 918, 1010, 1254, 1332, 1369
BseNI ACTGG 3 cut(s) 394, 738, 1315
BseRI GAGGAG 1 cut(s) 1431
BseXI GCAGC 1 cut(s) 1397
BseYI CCCAGC 1 cut(s) 984
BsgI GTGCAG 1 cut(s) 220
BshFI GGCC 3 cut(s) 50, 518, 1520
BsiHKAI GWGCWC 2 cut(s) 366, 1250
BsiSI CCGG 1 cut(s) 40
BslFI GGGAC 1 cut(s) 1333
BslI CCNNNNNNNGG 4 cut(s) 46, 848, 1083, 1415
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 1333
BsnI GGCC 3 cut(s) 50, 518, 1520
Bsp1286I GDGCHC 2 cut(s) 366, 1250
Bsp143I GATC 5 cut(s) 204, 511, 754, 1042, 1311
Bsp19I CCATGG 1 cut(s) 1139
BspACI CCGC 1 cut(s) 1425
BspANI GGCC 3 cut(s) 50, 518, 1520
BspCNI CTCAG 7 cut(s) 295, 804, 919, 1009, 1253, 1333, 1368
BspLI GGNNCC 2 cut(s) 471, 756
BspMAI CTGCAG 1 cut(s) 858
BspPI GGATC 4 cut(s) 519, 749, 762, 1306
BsrDI GCAATG 1 cut(s) 53
BsrI ACTGG 3 cut(s) 394, 738, 1315
BssECI CCNNGG 2 cut(s) 1139, 1521
BssMI GATC 5 cut(s) 204, 511, 754, 1042, 1311
BssT1I CCWWGG 2 cut(s) 1139, 1521
Bst4CI ACNGT 3 cut(s) 677, 749, 1133
Bst6I CTCTTC 3 cut(s) 183, 444, 1505
BstAPI GCANNNNNTGC 1 cut(s) 397
BstBAI YACGTR 1 cut(s) 937
BstDEI CTNAG 9 cut(s) 303, 723, 812, 927, 996, 1240, 1341, 1355, 1457
BstDSI CCRYGG 1 cut(s) 1139
BstENI CCTNNNNNAGG 1 cut(s) 1413
BstF5I GGATG 4 cut(s) 9, 570, 783, 1299
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 5 cut(s) 207, 514, 757, 1045, 1314
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 5 cut(s) 204, 511, 754, 1042, 1311
BstMWI GCNNNNNNNGC 4 cut(s) 118, 253, 397, 1306
BstNSI RCATGY 3 cut(s) 444, 919, 1531
BstSFI CTRYAG 1 cut(s) 854
BstV1I GCAGC 1 cut(s) 1397
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 2 cut(s) 511, 754
BstYI RGATCY 2 cut(s) 511, 754
BsuI GTATCC 1 cut(s) 1009
BsuRI GGCC 3 cut(s) 50, 518, 1520
BtgI CCRYGG 1 cut(s) 1139
BtrI CACGTC 1 cut(s) 974
BtsCI GGATG 4 cut(s) 9, 570, 783, 1299
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 3 cut(s) 376, 809, 1248
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 1 cut(s) 588
Csp6I GTAC 2 cut(s) 6, 1162
CviQI GTAC 2 cut(s) 6, 1162
DdeI CTNAG 9 cut(s) 303, 723, 812, 927, 996, 1240, 1341, 1355, 1457
DpnI GATC 5 cut(s) 206, 513, 756, 1044, 1313
DpnII GATC 5 cut(s) 204, 511, 754, 1042, 1311
DrdI GACNNNNNNGTC 1 cut(s) 1345
DseDI GACNNNNNNGTC 1 cut(s) 1345
EaeI YGGCCR 2 cut(s) 48, 516
Eam1104I CTCTTC 3 cut(s) 183, 444, 1505
EarI CTCTTC 3 cut(s) 183, 444, 1505
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 1139, 1521
Eco24I GRGCYC 1 cut(s) 366
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 3 cut(s) 207, 295, 1167
EcoICRI GAGCTC 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 1413
EcoT14I CCWWGG 2 cut(s) 1139, 1521
EcoT22I ATGCAT 1 cut(s) 829
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 2 cut(s) 1139, 1521
FaqI GGGAC 1 cut(s) 1333
FauI CCCGC 1 cut(s) 1418
FbaI TGATCA 2 cut(s) 204, 1042
FblI GTMKAC 1 cut(s) 1135
Fnu4HI GCNGC 1 cut(s) 1386
FokI GGATG 3 cut(s) 577, 790, 1306
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 1 cut(s) 1386
FspBI CTAG 4 cut(s) 65, 537, 798, 1112
FspI TGCGCA 1 cut(s) 587
GlaI GCGC 1 cut(s) 587
GluI GCNGC 1 cut(s) 1386
GsaI CCCAGC 1 cut(s) 988
HaeIII GGCC 3 cut(s) 50, 518, 1520
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 588
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HincII GTYRAC 4 cut(s) 924, 1136, 1153, 1227
HindII GTYRAC 4 cut(s) 924, 1136, 1153, 1227
HindIII AAGCTT 5 cut(s) 18, 170, 352, 876, 1459
HinfI GANTC 9 cut(s) 61, 260, 929, 1073, 1088, 1154, 1238, 1255, 1547
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 1553
Hpy166II GTNNAC 8 cut(s) 6, 128, 924, 1136, 1153, 1227, 1361, 1533
Hpy188I TCNGA 7 cut(s) 204, 304, 341, 754, 999, 1093, 1476
Hpy188III TCNNGA 6 cut(s) 293, 649, 1036, 1070, 1259, 1551
Hpy8I GTNNAC 8 cut(s) 6, 128, 924, 1136, 1153, 1227, 1361, 1533
HpyAV CCTTC 4 cut(s) 541, 646, 731, 1175
HpyCH4III ACNGT 3 cut(s) 677, 749, 1133
HpyCH4IV ACGT 3 cut(s) 719, 936, 973
HpyCH4V TGCA 9 cut(s) 237, 391, 568, 827, 856, 952, 1005, 1109, 1331
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 253, 397, 1306
HpyF3I CTNAG 9 cut(s) 303, 723, 812, 927, 996, 1240, 1341, 1355, 1457
HpySE526I ACGT 3 cut(s) 719, 936, 973
HspAI GCGC 1 cut(s) 586
Ksp22I TGATCA 2 cut(s) 204, 1042
Kzo9I GATC 5 cut(s) 204, 511, 754, 1042, 1311
LmnI GCTCC 4 cut(s) 24, 42, 341, 1253
Lsp1109I GCAGC 1 cut(s) 1397
LweI GCATC 1 cut(s) 555
MaeI CTAG 4 cut(s) 65, 537, 798, 1112
MaeII ACGT 3 cut(s) 719, 936, 973
MaeIII GTNAC 6 cut(s) 94, 265, 282, 414, 436, 1119
MalI GATC 5 cut(s) 206, 513, 756, 1044, 1313
MboI GATC 5 cut(s) 204, 511, 754, 1042, 1311
MboII GAAGA 5 cut(s) 200, 344, 461, 641, 1522
MflI RGATCY 2 cut(s) 511, 754
MhlI GDGCHC 2 cut(s) 366, 1250
MlsI TGGCCA 1 cut(s) 518
MluCI AATT 8 cut(s) 107, 208, 386, 656, 789, 1000, 1030, 1477
MluNI TGGCCA 1 cut(s) 518
MlyI GAGTC 4 cut(s) 254, 938, 1148, 1232
MmeI TCCRAC 1 cut(s) 782
Mox20I TGGCCA 1 cut(s) 518
Mph1103I ATGCAT 1 cut(s) 829
MroXI GAANNNNTTC 2 cut(s) 132, 870
MscI TGGCCA 1 cut(s) 518
MseI TTAA 6 cut(s) 318, 356, 624, 822, 1277, 1440
MslI CAYNNNNRTG 2 cut(s) 563, 939
Msp20I TGGCCA 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 1388
MspI CCGG 1 cut(s) 40
MwoI GCNNNNNNNGC 4 cut(s) 118, 253, 397, 1306
NcoI CCATGG 1 cut(s) 1139
NdeII GATC 5 cut(s) 204, 511, 754, 1042, 1311
NlaIV GGNNCC 2 cut(s) 471, 756
NmuCI GTSAC 2 cut(s) 436, 1119
NsbI TGCGCA 1 cut(s) 587
NsiI ATGCAT 1 cut(s) 829
NspI RCATGY 3 cut(s) 444, 919, 1531
PciI ACATGT 2 cut(s) 440, 1527
PdmI GAANNNNTTC 2 cut(s) 132, 870
PfeI GAWTC 5 cut(s) 61, 1073, 1088, 1255, 1547
PflFI GACNNNGTC 1 cut(s) 928
PkrI GCNGC 1 cut(s) 1387
PleI GAGTC 4 cut(s) 254, 937, 1148, 1232
PpsI GAGTC 4 cut(s) 254, 937, 1148, 1232
Ppu21I YACGTR 1 cut(s) 937
PscI ACATGT 2 cut(s) 440, 1527
PshBI ATTAAT 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 984
PspN4I GGNNCC 2 cut(s) 471, 756
PstI CTGCAG 1 cut(s) 858
PstNI CAGNNNCTG 1 cut(s) 263
PsuI RGATCY 2 cut(s) 511, 754
PsyI GACNNNGTC 1 cut(s) 928
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 2 cut(s) 7, 1163
RsaNI GTAC 2 cut(s) 6, 1162
RseI CAYNNNNRTG 2 cut(s) 563, 939
SacI GAGCTC 1 cut(s) 366
SalI GTCGAC 1 cut(s) 1134
SaqAI TTAA 6 cut(s) 318, 356, 624, 822, 1277, 1440
SatI GCNGC 1 cut(s) 1386
Sau3AI GATC 5 cut(s) 204, 511, 754, 1042, 1311
SchI GAGTC 4 cut(s) 254, 938, 1148, 1232
SduI GDGCHC 2 cut(s) 366, 1250
SfaNI GCATC 1 cut(s) 555
SfcI CTRYAG 1 cut(s) 854
SmiMI CAYNNNNRTG 2 cut(s) 563, 939
Sse9I AATT 8 cut(s) 107, 208, 386, 656, 789, 1000, 1030, 1477
SsiI CCGC 1 cut(s) 1425
SspI AATATT 1 cut(s) 870
SspMI CTAG 4 cut(s) 65, 537, 798, 1112
SstI GAGCTC 1 cut(s) 366
StyI CCWWGG 2 cut(s) 1139, 1521
TaaI ACNGT 3 cut(s) 677, 749, 1133
TaiI ACGT 3 cut(s) 722, 939, 976
TaqI TCGA 2 cut(s) 711, 1135
TasI AATT 8 cut(s) 107, 208, 386, 656, 789, 1000, 1030, 1477
TfiI GAWTC 5 cut(s) 61, 1073, 1088, 1255, 1547
Tru1I TTAA 6 cut(s) 318, 356, 624, 822, 1277, 1440
Tru9I TTAA 6 cut(s) 318, 356, 624, 822, 1277, 1440
TscAI CASTG 3 cut(s) 383, 816, 1248
TseFI GTSAC 2 cut(s) 436, 1119
TseI GCWGC 1 cut(s) 1385
Tsp45I GTSAC 2 cut(s) 436, 1119
TspDTI ATGAA 6 cut(s) 155, 462, 611, 980, 1229, 1423
TspRI CASTG 3 cut(s) 383, 816, 1248
Tth111I GACNNNGTC 1 cut(s) 928
VspI ATTAAT 1 cut(s) 318
XagI CCTNNNNNAGG 1 cut(s) 1413
XapI RAATTY 4 cut(s) 656, 789, 1030, 1477
XceI RCATGY 3 cut(s) 444, 919, 1531
XmiI GTMKAC 1 cut(s) 1135
XmnI GAANNNNTTC 2 cut(s) 132, 870
XspI CTAG 4 cut(s) 65, 537, 798, 1112
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.