Rw5G032970

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0021650
Physical Location & Seq
Reverse (-)
55925044 .. 55932264
7221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032970.1

Sequence Viewer

Length: 1629 bp
ATGACCGAGCAAATTGGTAGTACTACCATGCGAAGGGTAGCTTTTAAGGATGTTCCTTTGAATAATCTAAATGATCTGGTACGTAGTCAAATTAAAATTGCAAGTTTTAAAGCTGGAATGGGTGTTCCTTTGAAGACGAATCTAATGGAAGCTGTAAATGATACTGAAATTAAAGCATCATCCTTTCACGTTGAACTGGATGTGCCTATAAGGGAACTGAAGAAGAAGCTCTTTACGGATGGGGTGTCAATGCTTGTTGTAAATGGTAATGGAGGATGCGGGAAAACCAAAGTGGCAAAGCAGTTTTGTCAAGATGACGAAGTCAAAGATAAATTCAAAATGAATATCTTTTTTGTGACGGTTTCAAAACGACCGAATTTGGACTACATTATACACCAATTATATCAACAAATGGGTTCCGAAGCTTGTATTTTCCAGAACGATGTAAGTGCAGTTATGTGGCTAGAACATTTTCTAAGGGAAGCGGGGCAAAATCCTTTGTTGTTGGTCTTGGATGATGTTTGGCCTGGATCAGAATCTCTTCTTCAGAAGCTTGATGAATTGAATAAAATGCCAAATTACAAGATTTTGGTGACCTCAAGATTTGAATTTCCCGGATGCGGCTCTACATATTATTTGAGTTCGTCAAATAGTAAAGAGCCAATGGACCTTTTATATCAGAAACGGCTCACCACTCCGACTTCAGCTATTTCTATAGTCCCAAGGATGGAGAGGAGAGAGGCGATGGCTGATGATGAGTTACGTGCTGAACTTAAAGCTGAGGTGCAGGCAGTCAAGCGAGACTTGACAAGTAAACTAGATGGCATCAACCAACAACTTGTCTACCTCATACAAAGTAAAGATGAGAAGGGCCCGGAGCGCACAAAAGAAATTGAGGTTACACAAGGTTACACAAGACGCATGGGCGTTGATGCCATCACTGAAGACTTCAAGGTTACACAAGAACTTCAAATCCTAGCAACAGAGCATCAATCAATGAGGCAGTTTCGGGCATGGATCGGTCATCCAGACGATGGACGGCGCGACTTAGAAATCTTTGAAGCATCAAAGAAATGGTTGGAAGACTTGATGACCTCAGGCGTGTGGTTGAGTGACACGCATGTAGATGCTTTTTTGTACTTCGTTCGCAAGAATACACATCATCATTCCCAATCTACTCAATCTTGCATGATCCTGGATACTGTGTTTTGGGGTTGGATGAGTGGGCGATGGACTTCTTTCCAGAATAACCCCAACTCCTATGAGTGGGATTCAGATATTATGGGCTACCCCCTTGGCAAGTCGCCTCAATTCACCCGACCATGGAGTGAAGTTGACTATTTGTACATTCCGGTGAACGTTACCAACCAGCACTGGGTAGCTGTATGTGTAGACTTAATGAGGAGAAAGTTCACCATTTACGACTCGATGCTATCAGCAAATCGGGATGAGTTCATCTGTGACCTACTGAATCCCATTGCCACAATGTTTCCATCATTGCTTATCCAGTCGGGGTTTTATAAAATGAGGCCTGAGTTGTCCCCTCTGAACACACCGTTTCAGGTTGTACGTCTAGCAGAAAATATCCCACAACAATCGGAAAGCGGAGATTGCGGAGTGTTCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

542

Amino Acids

62.2

Weight (kDa)

5.71

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 69 - 201 1.6e-13 NB-ARC domain
Peptidase_C48 PF02902 369 - 542 2.5e-28 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021650)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002194.1_g000018
rosa_roxburghii Rroxscaffold_1G00027280
rosa_samantha Rh5BG360600 Rh5DG375400
rosa_wichuraiana Rw5G032970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1521
AccB7I CCANNNNNTGG 1 cut(s) 1034
AccI GTMKAC 2 cut(s) 843, 1392
AccII CGCG 1 cut(s) 1044
AciI CCGC 5 cut(s) 279, 485, 621, 1605, 1614
AclI AACGTT 1 cut(s) 1359
AclWI GGATC 3 cut(s) 538, 1025, 1186
AcsI RAATTY 3 cut(s) 332, 376, 608
AcuI CTGAAG 4 cut(s) 239, 530, 687, 963
AfaI GTAC 5 cut(s) 22, 81, 1139, 1346, 1569
AfiI CCNNNNNNNGG 7 cut(s) 33, 620, 727, 1034, 1266, 1323, 1375
AjnI CCWGG 2 cut(s) 526, 1194
AloI GAACNNNNNNTCC 4 cut(s) 108, 140, 957, 989
AluBI AGCT 9 cut(s) 41, 113, 152, 229, 425, 553, 707, 779, 1382
AluI AGCT 9 cut(s) 41, 113, 152, 229, 425, 553, 707, 779, 1382
Alw26I GTCTC 1 cut(s) 795
AlwI GGATC 3 cut(s) 538, 1025, 1186
AoxI GGCC 3 cut(s) 524, 871, 1529
ApaI GGGCCC 1 cut(s) 875
ApoI RAATTY 3 cut(s) 332, 376, 608
Asp700I GAANNNNTTC 2 cut(s) 471, 540
AspLEI GCGC 2 cut(s) 882, 1044
AspS9I GGNCC 3 cut(s) 667, 871, 872
AsuC2I CCSGG 2 cut(s) 615, 875
AsuHPI GGTGA 5 cut(s) 604, 682, 1306, 1366, 1405
AvaII GGWCC 1 cut(s) 667
AxyI CCTNAGG 1 cut(s) 1096
BaeGI GKGCMC 1 cut(s) 875
BanII GRGCYC 1 cut(s) 875
BbsI GAAGAC 3 cut(s) 140, 951, 1089
BbvCI CCTCAGC 1 cut(s) 780
BccI CCATC 8 cut(s) 233, 721, 739, 815, 944, 1028, 1224, 1501
BceAI ACGGC 2 cut(s) 701, 1055
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 2 cut(s) 528, 1196
BciVI GTATCC 1 cut(s) 1192
BcnI CCSGG 2 cut(s) 615, 875
BcoDI GTCTC 1 cut(s) 795
BfaI CTAG 4 cut(s) 464, 818, 977, 1574
BfmI CTRYAG 1 cut(s) 714
BfuI GTATCC 1 cut(s) 1192
BisI GCNGC 1 cut(s) 622
BlsI GCNGC 1 cut(s) 623
BmcAI AGTACT 1 cut(s) 22
Bme1390I CCNGG 4 cut(s) 528, 615, 875, 1196
Bme18I GGWCC 1 cut(s) 667
BmgT120I GGNCC 3 cut(s) 667, 871, 872
BmiI GGNNCC 2 cut(s) 418, 873
BmrFI CCNGG 4 cut(s) 528, 615, 875, 1196
BmrI ACTGGG 1 cut(s) 1384
BmsI GCATC 9 cut(s) 185, 266, 608, 834, 922, 997, 1073, 1117, 1419
BmuI ACTGGG 1 cut(s) 1384
BpiI GAAGAC 3 cut(s) 140, 951, 1089
Bpu10I CCTNAGC 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 2 cut(s) 615, 875
BsaAI YACGTR 2 cut(s) 83, 764
BsaBI GATNNNNATC 1 cut(s) 535
BsaJI CCNNGG 3 cut(s) 722, 1294, 1322
BsaWI WCCGGW 1 cut(s) 1351
BsaXI ACNNNNNCTCC 3 cut(s) 1241, 1271, 1608
Bsc4I CCNNNNNNNGG 7 cut(s) 33, 620, 727, 1034, 1266, 1323, 1375
Bse1I ACTGG 3 cut(s) 201, 1379, 1507
Bse21I CCTNAGG 1 cut(s) 1096
Bse3DI GCAATG 2 cut(s) 1476, 1496
Bse8I GATNNNNATC 1 cut(s) 535
BseBI CCWGG 2 cut(s) 528, 1196
BseDI CCNNGG 3 cut(s) 722, 1294, 1322
BseJI GATNNNNATC 1 cut(s) 535
BseLI CCNNNNNNNGG 7 cut(s) 33, 620, 727, 1034, 1266, 1323, 1375
BseMI GCAATG 2 cut(s) 1476, 1496
BseMII CTCAG 3 cut(s) 771, 1110, 1524
BseNI ACTGG 3 cut(s) 201, 1379, 1507
BseRI GAGGAG 2 cut(s) 748, 1417
BseSI GKGCMC 1 cut(s) 875
BsgI GTGCAG 2 cut(s) 471, 806
Bsh1236I CGCG 1 cut(s) 1044
Bsh1285I CGRYCG 1 cut(s) 374
BshFI GGCC 3 cut(s) 526, 873, 1531
BsiEI CGRYCG 1 cut(s) 374
BsiSI CCGG 3 cut(s) 615, 875, 1352
BslFI GGGAC 2 cut(s) 704, 1525
BslI CCNNNNNNNGG 7 cut(s) 33, 620, 727, 1034, 1266, 1323, 1375
BsmAI GTCTC 1 cut(s) 795
BsmFI GGGAC 2 cut(s) 704, 1525
BsnI GGCC 3 cut(s) 526, 873, 1531
Bsp120I GGGCCC 1 cut(s) 871
Bsp1286I GDGCHC 1 cut(s) 875
Bsp1407I TGTACA 1 cut(s) 1344
Bsp143I GATC 4 cut(s) 73, 530, 1017, 1191
Bsp19I CCATGG 1 cut(s) 1322
BspACI CCGC 5 cut(s) 279, 485, 621, 1605, 1614
BspANI GGCC 3 cut(s) 526, 873, 1531
BspCNI CTCAG 3 cut(s) 772, 1109, 1525
BspFNI CGCG 1 cut(s) 1044
BspLI GGNNCC 2 cut(s) 418, 873
BspPI GGATC 3 cut(s) 538, 1025, 1186
BsrDI GCAATG 2 cut(s) 1476, 1496
BsrGI TGTACA 1 cut(s) 1344
BsrI ACTGG 3 cut(s) 201, 1379, 1507
BssECI CCNNGG 3 cut(s) 722, 1294, 1322
BssMI GATC 4 cut(s) 73, 530, 1017, 1191
BssT1I CCWWGG 3 cut(s) 722, 1294, 1322
Bst2UI CCWGG 2 cut(s) 528, 1196
Bst4CI ACNGT 3 cut(s) 361, 1204, 1557
Bst6I CTCTTC 1 cut(s) 546
BstAUI TGTACA 1 cut(s) 1344
BstBAI YACGTR 2 cut(s) 83, 764
BstC8I GCNNGC 1 cut(s) 789
BstDEI CTNAG 5 cut(s) 476, 780, 1048, 1096, 1533
BstDSI CCRYGG 1 cut(s) 1322
BstEII GGTNACC 1 cut(s) 592
BstFNI CGCG 1 cut(s) 1044
BstHHI GCGC 2 cut(s) 882, 1044
BstKTI GATC 4 cut(s) 76, 533, 1020, 1194
BstMAI GTCTC 1 cut(s) 795
BstMBI GATC 4 cut(s) 73, 530, 1017, 1191
BstMCI CGRYCG 1 cut(s) 374
BstMWI GCNNNNNNNGC 2 cut(s) 879, 1611
BstNI CCWGG 2 cut(s) 528, 1196
BstNSI RCATGY 1 cut(s) 1124
BstPI GGTNACC 1 cut(s) 592
BstSCI CCNGG 4 cut(s) 526, 613, 873, 1194
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 875
BstSNI TACGTA 1 cut(s) 83
BstUI CGCG 1 cut(s) 1044
BstV2I GAAGAC 3 cut(s) 140, 951, 1089
Bsu36I CCTNAGG 1 cut(s) 1096
BsuI GTATCC 1 cut(s) 1192
BsuRI GGCC 3 cut(s) 526, 873, 1531
BtgI CCRYGG 1 cut(s) 1322
BtgZI GCGATG 2 cut(s) 758, 1243
BtsIMutI CAGTG 2 cut(s) 939, 1372
Cac8I GCNNGC 1 cut(s) 789
CfoI GCGC 2 cut(s) 882, 1044
Cfr13I GGNCC 3 cut(s) 667, 871, 872
CseI GACGC 1 cut(s) 927
Csp6I GTAC 5 cut(s) 21, 80, 1138, 1345, 1568
CviAII CATG 6 cut(s) 28, 922, 1014, 1121, 1189, 1323
CviQI GTAC 5 cut(s) 21, 80, 1138, 1345, 1568
DdeI CTNAG 5 cut(s) 476, 780, 1048, 1096, 1533
DpnI GATC 4 cut(s) 75, 532, 1019, 1193
DpnII GATC 4 cut(s) 73, 530, 1017, 1191
DraI TTTAAA 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Eco105I TACGTA 1 cut(s) 83
Eco130I CCWWGG 3 cut(s) 722, 1294, 1322
Eco147I AGGCCT 1 cut(s) 1531
Eco24I GRGCYC 1 cut(s) 875
Eco47I GGWCC 1 cut(s) 667
Eco57I CTGAAG 4 cut(s) 239, 530, 687, 963
Eco81I CCTNAGG 1 cut(s) 1096
Eco91I GGTNACC 1 cut(s) 592
EcoO109I RGGNCCY 1 cut(s) 871
EcoO65I GGTNACC 1 cut(s) 592
EcoRII CCWGG 2 cut(s) 526, 1194
EcoT14I CCWWGG 3 cut(s) 722, 1294, 1322
EcoT38I GRGCYC 1 cut(s) 875
ErhI CCWWGG 3 cut(s) 722, 1294, 1322
FaeI CATG 6 cut(s) 31, 925, 1017, 1124, 1192, 1326
FalI AAGNNNNNCTT 6 cut(s) 25, 57, 215, 247, 788, 820
FaqI GGGAC 2 cut(s) 704, 1525
FatI CATG 6 cut(s) 27, 921, 1013, 1120, 1188, 1322
FauI CCCGC 2 cut(s) 272, 478
FblI GTMKAC 2 cut(s) 843, 1392
Fnu4HI GCNGC 1 cut(s) 622
FriOI GRGCYC 1 cut(s) 875
Fsp4HI GCNGC 1 cut(s) 622
FspBI CTAG 4 cut(s) 464, 818, 977, 1574
GlaI GCGC 2 cut(s) 881, 1043
GluI GCNGC 1 cut(s) 622
HaeIII GGCC 3 cut(s) 526, 873, 1531
HapII CCGG 3 cut(s) 615, 875, 1352
HgaI GACGC 1 cut(s) 927
HhaI GCGC 2 cut(s) 882, 1044
Hin1II CATG 6 cut(s) 31, 925, 1017, 1124, 1192, 1326
Hin6I GCGC 2 cut(s) 880, 1042
HinP1I GCGC 2 cut(s) 880, 1042
HincII GTYRAC 1 cut(s) 1336
HindII GTYRAC 1 cut(s) 1336
HindIII AAGCTT 2 cut(s) 423, 551
HinfI GANTC 5 cut(s) 139, 536, 1271, 1424, 1471
HpaII CCGG 3 cut(s) 615, 875, 1352
HphI GGTGA 5 cut(s) 604, 682, 1306, 1366, 1405
Hpy166II GTNNAC 6 cut(s) 815, 844, 1336, 1357, 1393, 1413
Hpy188I TCNGA 8 cut(s) 421, 535, 549, 681, 699, 1276, 1548, 1600
Hpy188III TCNNGA 6 cut(s) 311, 436, 600, 1028, 1243, 1445
Hpy8I GTNNAC 6 cut(s) 815, 844, 1336, 1357, 1393, 1413
HpyAV CCTTC 2 cut(s) 27, 862
HpyCH4III ACNGT 3 cut(s) 361, 1204, 1557
HpyCH4IV ACGT 5 cut(s) 82, 189, 763, 1359, 1570
HpyCH4V TGCA 4 cut(s) 101, 452, 787, 1188
HpyF10VI GCNNNNNNNGC 2 cut(s) 879, 1611
HpyF3I CTNAG 5 cut(s) 476, 780, 1048, 1096, 1533
HpySE526I ACGT 5 cut(s) 82, 189, 763, 1359, 1570
Hsp92II CATG 6 cut(s) 31, 925, 1017, 1124, 1192, 1326
HspAI GCGC 2 cut(s) 880, 1042
Kzo9I GATC 4 cut(s) 73, 530, 1017, 1191
LmnI GCTCC 1 cut(s) 877
LweI GCATC 9 cut(s) 185, 266, 608, 834, 922, 997, 1073, 1117, 1419
MaeI CTAG 4 cut(s) 464, 818, 977, 1574
MaeII ACGT 5 cut(s) 82, 189, 763, 1359, 1570
MaeIII GTNAC 9 cut(s) 355, 592, 759, 898, 908, 955, 1112, 1360, 1460
MalI GATC 4 cut(s) 75, 532, 1019, 1193
MboI GATC 4 cut(s) 73, 530, 1017, 1191
MboII GAAGA 7 cut(s) 145, 232, 235, 533, 536, 956, 1094
MhlI GDGCHC 1 cut(s) 875
MlyI GAGTC 1 cut(s) 1418
MmeI TCCRAC 3 cut(s) 722, 1059, 1196
MroXI GAANNNNTTC 2 cut(s) 471, 540
MseI TTAA 6 cut(s) 45, 93, 108, 171, 774, 1397
MslI CAYNNNNRTG 2 cut(s) 1125, 1352
MspI CCGG 3 cut(s) 615, 875, 1352
MspR9I CCNGG 4 cut(s) 528, 615, 875, 1196
MvaI CCWGG 2 cut(s) 528, 1196
MvnI CGCG 1 cut(s) 1044
MwoI GCNNNNNNNGC 2 cut(s) 879, 1611
NciI CCSGG 2 cut(s) 615, 875
NcoI CCATGG 1 cut(s) 1322
NdeII GATC 4 cut(s) 73, 530, 1017, 1191
NlaIII CATG 6 cut(s) 31, 925, 1017, 1124, 1192, 1326
NlaIV GGNNCC 2 cut(s) 418, 873
NmuCI GTSAC 4 cut(s) 355, 592, 1112, 1460
NspI RCATGY 1 cut(s) 1124
PceI AGGCCT 1 cut(s) 1531
PdmI GAANNNNTTC 2 cut(s) 471, 540
PfeI GAWTC 4 cut(s) 139, 536, 1271, 1471
PflFI GACNNNGTC 1 cut(s) 320
PflMI CCANNNNNTGG 1 cut(s) 1034
PfoI TCCNGGA 2 cut(s) 613, 1194
PkrI GCNGC 1 cut(s) 623
PleI GAGTC 1 cut(s) 1418
PpsI GAGTC 1 cut(s) 1418
Ppu21I YACGTR 2 cut(s) 83, 764
PsiI TTATAA 1 cut(s) 1521
Psp1406I AACGTT 1 cut(s) 1359
Psp6I CCWGG 2 cut(s) 526, 1194
PspEI GGTNACC 1 cut(s) 592
PspGI CCWGG 2 cut(s) 526, 1194
PspN4I GGNNCC 2 cut(s) 418, 873
PspOMI GGGCCC 1 cut(s) 871
PspPI GGNCC 3 cut(s) 667, 871, 872
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 5 cut(s) 22, 81, 1139, 1346, 1569
RsaNI GTAC 5 cut(s) 21, 80, 1138, 1345, 1568
RseI CAYNNNNRTG 2 cut(s) 1125, 1352
SaqAI TTAA 6 cut(s) 45, 93, 108, 171, 774, 1397
SatI GCNGC 1 cut(s) 622
Sau3AI GATC 4 cut(s) 73, 530, 1017, 1191
Sau96I GGNCC 3 cut(s) 667, 871, 872
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 1 cut(s) 1418
ScrFI CCNGG 4 cut(s) 528, 615, 875, 1196
SduI GDGCHC 1 cut(s) 875
SfaNI GCATC 9 cut(s) 185, 266, 608, 834, 922, 997, 1073, 1117, 1419
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 1 cut(s) 667
SmiMI CAYNNNNRTG 2 cut(s) 1125, 1352
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
SnaBI TACGTA 1 cut(s) 83
SseBI AGGCCT 1 cut(s) 1531
SsiI CCGC 5 cut(s) 279, 485, 621, 1605, 1614
SspMI CTAG 4 cut(s) 464, 818, 977, 1574
StuI AGGCCT 1 cut(s) 1531
StyD4I CCNGG 4 cut(s) 526, 613, 873, 1194
StyI CCWWGG 3 cut(s) 722, 1294, 1322
TaaI ACNGT 3 cut(s) 361, 1204, 1557
TaiI ACGT 5 cut(s) 85, 192, 766, 1362, 1573
TaqI TCGA 1 cut(s) 1427
TaqII GACCGA 3 cut(s) 20, 388, 1010
TatI WGTACW 3 cut(s) 20, 1137, 1344
TauI GCSGC 1 cut(s) 624
TfiI GAWTC 4 cut(s) 139, 536, 1271, 1471
Tru1I TTAA 6 cut(s) 45, 93, 108, 171, 774, 1397
Tru9I TTAA 6 cut(s) 45, 93, 108, 171, 774, 1397
TscAI CASTG 2 cut(s) 946, 1379
TseFI GTSAC 4 cut(s) 355, 592, 1112, 1460
Tsp45I GTSAC 4 cut(s) 355, 592, 1112, 1460
TspDTI ATGAA 3 cut(s) 356, 573, 1444
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 2 cut(s) 946, 1379
Tth111I GACNNNGTC 1 cut(s) 320
Van91I CCANNNNNTGG 1 cut(s) 1034
VpaK11BI GGWCC 1 cut(s) 667
XapI RAATTY 3 cut(s) 332, 376, 608
XceI RCATGY 1 cut(s) 1124
XmiI GTMKAC 2 cut(s) 843, 1392
XmnI GAANNNNTTC 2 cut(s) 471, 540
XspI CTAG 4 cut(s) 464, 818, 977, 1574
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.