Rh5DG375400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0021650
Physical Location & Seq
Reverse (-)
57047033 .. 57052931
5899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG375400.1

Sequence Viewer

Length: 1026 bp
ATGGCTGATGATGAGTTACGTGCTGAACTTAAAGCTGAGGTGCAGGCAGTCAAGCGAGACTTGACAAGTAAACTAGATGGCATCAACCAACAACTTGTCTACCTCATACAAAGTAAAGATGAGAAGGGCCCGGAGCGCACAAAAGAAATTGAGGTTACACAAGGTTACACAAGACGCATGGACGTTGATGCCATCACTGAAGACTTCAAGGTTACACAAGAACTTCAAATCCTAGCAACAGAGCAACAATCAATGAGGCAGTTTCGGGCATGGATCGGTCATCCAGACGATGGACGGCGCGACTTAGAAATCTTTGAAGCATCAAAGAAATGGTTGGAAGACTTGATGACCTCAGGCGTGTGGTTGAGTGACACGCATGTAGATGCTTTTTTGTACTTCGTTCGCAAGAATACACATCATCATTCCCAATCTACTCAGTCTTGCATGATCCTGGATACTGTGTTTTGGGGTTGGATGAGTTGGCGATGGACTTCTTTCCAGAATAACCCCAACTCCTATGAGTGGGATTCAGATATTATGGGCTACCCCCTTGGCAAGTCGCCTCAATTCACCCGACCATGGAGTGAAGTTGACTATTTGTACATTCCGGTGAACGTTACCAACCAGCACTGGGTAGCTGTATGTGTAGACTTAATGAGGAGAAAGTTCACCATTTACGACTCGATGCTATCAGCAAATCGGGATGAGTTCATCTGTGACCTACTGAATCCCATTGCCACAATGTTTCCATCATTGCTTATCCAGTCGGGGTTTTATAAAATGAGGCCTGAGTTGTCCCCTCTGAACACACCGTTTCAGGTTGTACGTCTAGCAGAAAATATCCCACAACAATCGGAAAGCGGAGATTGCGGAGTGTTCGTGCTTAAATTTGTGGAGTATTTGAGCTCCGGGCAGCAATTAAGTTTCAGACAAGAAGACATACCGGCATTTAGGGAGCGAATGGCCTTCCTAATGTTTGCCATGAGTCATGACATTGGTGGCAAAATAAATACACTTGTGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

341

Amino Acids

39.71

Weight (kDa)

5.12

Isoelectric Point (pI)

49.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 121 - 329 6.7e-38 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021650)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002194.1_g000018
rosa_roxburghii Rroxscaffold_1G00027280
rosa_samantha Rh5BG360600 Rh5DG375400
rosa_wichuraiana Rw5G032970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 777
AccB7I CCANNNNNTGG 1 cut(s) 290
AccI GTMKAC 2 cut(s) 99, 648
AccII CGCG 1 cut(s) 300
AciI CCGC 2 cut(s) 861, 870
AclI AACGTT 1 cut(s) 615
AclWI GGATC 2 cut(s) 281, 442
AcsI RAATTY 1 cut(s) 887
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 3 cut(s) 395, 602, 825
AfiI CCNNNNNNNGG 4 cut(s) 290, 522, 579, 631
AgsI TTSAA 3 cut(s) 208, 227, 317
AjnI CCWGG 1 cut(s) 450
AloI GAACNNNNNNTCC 2 cut(s) 213, 245
AluBI AGCT 3 cut(s) 35, 638, 906
AluI AGCT 3 cut(s) 35, 638, 906
Alw21I GWGCWC 1 cut(s) 908
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 2 cut(s) 281, 442
AoxI GGCC 3 cut(s) 127, 785, 963
ApaI GGGCCC 1 cut(s) 131
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 1 cut(s) 887
AspLEI GCGC 2 cut(s) 138, 300
AspS9I GGNCC 2 cut(s) 127, 128
AsuC2I CCSGG 2 cut(s) 131, 910
AsuHPI GGTGA 3 cut(s) 562, 622, 661
AxyI CCTNAGG 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 131
BanII GRGCYC 2 cut(s) 131, 908
BbsI GAAGAC 3 cut(s) 207, 345, 942
Bbv12I GWGCWC 1 cut(s) 908
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 925
BccI CCATC 5 cut(s) 71, 200, 284, 480, 757
BceAI ACGGC 1 cut(s) 311
BciT130I CCWGG 1 cut(s) 452
BciVI GTATCC 1 cut(s) 448
BcnI CCSGG 2 cut(s) 131, 910
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 3 cut(s) 74, 233, 830
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 1 cut(s) 914
BlsI GCNGC 1 cut(s) 915
Bme1390I CCNGG 3 cut(s) 131, 452, 910
BmgT120I GGNCC 2 cut(s) 127, 128
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 3 cut(s) 131, 452, 910
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 5 cut(s) 90, 178, 329, 373, 675
BmuI ACTGGG 1 cut(s) 640
BpiI GAAGAC 3 cut(s) 207, 345, 942
Bpu10I CCTNAGC 1 cut(s) 36
BpuMI CCSGG 2 cut(s) 131, 910
BsaAI YACGTR 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 550, 578
BsaWI WCCGGW 1 cut(s) 607
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 864, 894
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 522, 579, 631
Bse118I RCCGGY 1 cut(s) 943
Bse1I ACTGG 2 cut(s) 635, 763
Bse21I CCTNAGG 1 cut(s) 352
Bse3DI GCAATG 2 cut(s) 732, 752
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 2 cut(s) 550, 578
BseGI GGATG 3 cut(s) 280, 480, 709
BseLI CCNNNNNNNGG 4 cut(s) 290, 522, 579, 631
BseMI GCAATG 2 cut(s) 732, 752
BseMII CTCAG 4 cut(s) 27, 366, 449, 780
BseNI ACTGG 2 cut(s) 635, 763
BseRI GAGGAG 1 cut(s) 673
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 1 cut(s) 925
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 129, 787, 965
BsiHKAI GWGCWC 1 cut(s) 908
BsiSI CCGG 4 cut(s) 131, 608, 909, 944
BslFI GGGAC 1 cut(s) 781
BslI CCNNNNNNNGG 4 cut(s) 290, 522, 579, 631
BsmAI GTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 781
BsnI GGCC 3 cut(s) 129, 787, 965
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 131, 908
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 2 cut(s) 273, 447
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 2 cut(s) 861, 870
BspANI GGCC 3 cut(s) 129, 787, 965
BspCNI CTCAG 4 cut(s) 28, 365, 448, 781
BspFNI CGCG 1 cut(s) 300
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 2 cut(s) 281, 442
BsrDI GCAATG 2 cut(s) 732, 752
BsrFI RCCGGY 1 cut(s) 943
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 2 cut(s) 635, 763
BssAI RCCGGY 1 cut(s) 943
BssECI CCNNGG 2 cut(s) 550, 578
BssMI GATC 2 cut(s) 273, 447
BssT1I CCWWGG 2 cut(s) 550, 578
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 2 cut(s) 460, 813
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 6 cut(s) 36, 304, 352, 435, 789, 1023
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 3 cut(s) 280, 480, 709
BstFNI CGCG 1 cut(s) 300
BstHHI GCGC 2 cut(s) 138, 300
BstKTI GATC 2 cut(s) 276, 450
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 2 cut(s) 273, 447
BstMWI GCNNNNNNNGC 2 cut(s) 135, 867
BstNI CCWGG 1 cut(s) 452
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 3 cut(s) 129, 450, 908
BstSLI GKGCMC 1 cut(s) 131
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 925
BstV2I GAAGAC 3 cut(s) 207, 345, 942
Bsu36I CCTNAGG 1 cut(s) 352
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 3 cut(s) 129, 787, 965
BtgI CCRYGG 1 cut(s) 578
BtgZI GCGATG 1 cut(s) 499
BtsCI GGATG 3 cut(s) 280, 480, 709
BtsIMutI CAGTG 2 cut(s) 195, 628
Cac8I GCNNGC 1 cut(s) 45
CciI TCATGA 1 cut(s) 988
CfoI GCGC 2 cut(s) 138, 300
Cfr10I RCCGGY 1 cut(s) 943
Cfr13I GGNCC 2 cut(s) 127, 128
CseI GACGC 1 cut(s) 183
Csp6I GTAC 3 cut(s) 394, 601, 824
CviAII CATG 7 cut(s) 178, 270, 377, 445, 579, 982, 989
CviJI RGCY 8 cut(s) 5, 35, 129, 543, 638, 787, 906, 965
CviKI_1 RGCY 8 cut(s) 5, 35, 129, 543, 638, 787, 906, 965
CviQI GTAC 3 cut(s) 394, 601, 824
DdeI CTNAG 6 cut(s) 36, 304, 352, 435, 789, 1023
DpnI GATC 2 cut(s) 275, 449
DpnII GATC 2 cut(s) 273, 447
Ecl136II GAGCTC 1 cut(s) 906
Eco130I CCWWGG 2 cut(s) 550, 578
Eco147I AGGCCT 1 cut(s) 787
Eco24I GRGCYC 2 cut(s) 131, 908
Eco53kI GAGCTC 1 cut(s) 906
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 906
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 450
EcoT14I CCWWGG 2 cut(s) 550, 578
EcoT38I GRGCYC 2 cut(s) 131, 908
ErhI CCWWGG 2 cut(s) 550, 578
FaeI CATG 7 cut(s) 181, 273, 380, 448, 582, 985, 992
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FaqI GGGAC 1 cut(s) 781
FatI CATG 7 cut(s) 177, 269, 376, 444, 578, 981, 988
FblI GTMKAC 2 cut(s) 99, 648
Fnu4HI GCNGC 1 cut(s) 914
FokI GGATG 3 cut(s) 267, 487, 716
FriOI GRGCYC 2 cut(s) 131, 908
Fsp4HI GCNGC 1 cut(s) 914
FspBI CTAG 3 cut(s) 74, 233, 830
GlaI GCGC 2 cut(s) 137, 299
GluI GCNGC 1 cut(s) 914
HaeIII GGCC 3 cut(s) 129, 787, 965
HapII CCGG 4 cut(s) 131, 608, 909, 944
HgaI GACGC 1 cut(s) 183
HhaI GCGC 2 cut(s) 138, 300
Hin1II CATG 7 cut(s) 181, 273, 380, 448, 582, 985, 992
Hin6I GCGC 2 cut(s) 136, 298
HinP1I GCGC 2 cut(s) 136, 298
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HinfI GANTC 4 cut(s) 527, 680, 727, 985
HpaII CCGG 4 cut(s) 131, 608, 909, 944
HphI GGTGA 3 cut(s) 562, 622, 661
Hpy166II GTNNAC 6 cut(s) 71, 100, 592, 613, 649, 669
Hpy188I TCNGA 4 cut(s) 532, 804, 856, 929
Hpy188III TCNNGA 4 cut(s) 284, 499, 701, 989
Hpy8I GTNNAC 6 cut(s) 71, 100, 592, 613, 649, 669
HpyAV CCTTC 2 cut(s) 118, 976
HpyCH4III ACNGT 2 cut(s) 460, 813
HpyCH4IV ACGT 4 cut(s) 19, 183, 615, 826
HpyCH4V TGCA 2 cut(s) 43, 444
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 867
HpyF3I CTNAG 6 cut(s) 36, 304, 352, 435, 789, 1023
HpySE526I ACGT 4 cut(s) 19, 183, 615, 826
Hsp92II CATG 7 cut(s) 181, 273, 380, 448, 582, 985, 992
HspAI GCGC 2 cut(s) 136, 298
Kzo9I GATC 2 cut(s) 273, 447
LmnI GCTCC 3 cut(s) 133, 911, 955
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 5 cut(s) 90, 178, 329, 373, 675
MaeI CTAG 3 cut(s) 74, 233, 830
MaeII ACGT 4 cut(s) 19, 183, 615, 826
MaeIII GTNAC 7 cut(s) 15, 154, 164, 211, 368, 616, 716
MalI GATC 2 cut(s) 275, 449
MboI GATC 2 cut(s) 273, 447
MboII GAAGA 3 cut(s) 212, 350, 947
MhlI GDGCHC 2 cut(s) 131, 908
MluCI AATT 4 cut(s) 147, 566, 887, 917
MlyI GAGTC 2 cut(s) 674, 994
MmeI TCCRAC 2 cut(s) 315, 452
MnlI CCTC 9 cut(s) 31, 113, 145, 249, 361, 573, 651, 777, 810
MseI TTAA 4 cut(s) 30, 653, 885, 920
MslI CAYNNNNRTG 2 cut(s) 381, 608
MspI CCGG 4 cut(s) 131, 608, 909, 944
MspR9I CCNGG 3 cut(s) 131, 452, 910
MvaI CCWGG 1 cut(s) 452
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 2 cut(s) 135, 867
NciI CCSGG 2 cut(s) 131, 910
NcoI CCATGG 1 cut(s) 578
NdeII GATC 2 cut(s) 273, 447
NlaIII CATG 7 cut(s) 181, 273, 380, 448, 582, 985, 992
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 2 cut(s) 368, 716
NspI RCATGY 1 cut(s) 380
PagI TCATGA 1 cut(s) 988
PceI AGGCCT 1 cut(s) 787
PfeI GAWTC 2 cut(s) 527, 727
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 450
PkrI GCNGC 1 cut(s) 915
PleI GAGTC 2 cut(s) 674, 993
PpsI GAGTC 2 cut(s) 674, 993
Ppu21I YACGTR 1 cut(s) 20
PsiI TTATAA 1 cut(s) 777
Psp124BI GAGCTC 1 cut(s) 908
Psp1406I AACGTT 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 450
PspGI CCWGG 1 cut(s) 450
PspN4I GGNNCC 1 cut(s) 129
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 2 cut(s) 127, 128
RsaI GTAC 3 cut(s) 395, 602, 825
RsaNI GTAC 3 cut(s) 394, 601, 824
RseI CAYNNNNRTG 2 cut(s) 381, 608
SacI GAGCTC 1 cut(s) 908
SaqAI TTAA 4 cut(s) 30, 653, 885, 920
SatI GCNGC 1 cut(s) 914
Sau3AI GATC 2 cut(s) 273, 447
Sau96I GGNCC 2 cut(s) 127, 128
SchI GAGTC 2 cut(s) 674, 994
ScrFI CCNGG 3 cut(s) 131, 452, 910
SduI GDGCHC 2 cut(s) 131, 908
SfaNI GCATC 5 cut(s) 90, 178, 329, 373, 675
SmiMI CAYNNNNRTG 2 cut(s) 381, 608
Sse9I AATT 4 cut(s) 147, 566, 887, 917
SseBI AGGCCT 1 cut(s) 787
SsiI CCGC 2 cut(s) 861, 870
SspMI CTAG 3 cut(s) 74, 233, 830
SstI GAGCTC 1 cut(s) 908
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 3 cut(s) 129, 450, 908
StyI CCWWGG 2 cut(s) 550, 578
TaaI ACNGT 2 cut(s) 460, 813
TaiI ACGT 4 cut(s) 22, 186, 618, 829
TaqI TCGA 1 cut(s) 683
TaqII GACCGA 1 cut(s) 266
TasI AATT 4 cut(s) 147, 566, 887, 917
TatI WGTACW 2 cut(s) 393, 600
TfiI GAWTC 2 cut(s) 527, 727
Tru1I TTAA 4 cut(s) 30, 653, 885, 920
Tru9I TTAA 4 cut(s) 30, 653, 885, 920
TscAI CASTG 2 cut(s) 202, 635
TseFI GTSAC 2 cut(s) 368, 716
TseI GCWGC 1 cut(s) 913
Tsp45I GTSAC 2 cut(s) 368, 716
TspDTI ATGAA 1 cut(s) 700
TspRI CASTG 2 cut(s) 202, 635
Van91I CCANNNNNTGG 1 cut(s) 290
XapI RAATTY 1 cut(s) 887
XceI RCATGY 1 cut(s) 380
XmiI GTMKAC 2 cut(s) 99, 648
XspI CTAG 3 cut(s) 74, 233, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.