Rh5BG360600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0021650
Physical Location & Seq
Reverse (-)
56327847 .. 56333880
6034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG360600.1

Sequence Viewer

Length: 1356 bp
ATGGGTTCAGAGGCTTGTATTTTCCAGAACGATGTAAGTGCAGTTATGTGGCTAGAACATTTTCTAAGGGAAGCGGGGCAAAATCCTTTGTTGTTGGTCTTGGATGATGTTTGGCCTGGATCAGAATCTCTTCTTCAGAAGCTTGATGAATTGAATAAAATGCCAAATTACAAGATTTTGGTGACCTCAAGATTTGAATTTCCCGGATGCGGCTCTACATATTATTTGAGTTCGTCAAATAGTAAAGAGCCAATGGACCTTTTATATCAGAAACGGCTCACCACTCCGACTTCAGCTATTTCTATAGTCCCAAGGATGGAGAGGAGAGAGGCGATGGCTGATGATGAGTTACGTGCTGAACTTAAAGCTGAGGTGCAGGCAGTCAAGCGAGACTTGACAAGTAAACTAGATGGCATCAACCAACAACTTGTCTACCTCATACAAAGTAAAGATGAGAAGGGCCCGGAGCGCACAAAAGAAATTGAGGTTACACAAGGTTACACAAGACGCATGGACGTTGATGCCATCACTGAAGACTTCAAGGTTACACAAGAACTTCAAATCCTAGCAACAGAGCAACAATCAATGAGGCAGTTTCGGGCATGGATCGGTCATCCAGACGATGGACGGCGCGACTTAGAAATCTTCGAAGCATCAAAGAAATGGTTGGAAGACTTGATGACCCCAGGCGTGTGGTTGAGTGACACGCATGTAGATGCTTTTTTGTACTTCGTTCGCAAGAATACACATCATTCCCAATCTACTCAATCTTGCATGATCCTGGATACCGTGTTTTGGGGTTGGATGAGTGGGCGATGGACTTCTTTCCAGAATAACCCCAACTCCTATGAGTGGGATTCAGATATTATGGGCTACCCCCTTGGCAAGTCGCCTCAATTCACCCGACTATGGAGTGAAGTTGACTGTTTGTACATTCCGGTGAACGTTACCAACCAGCACTGGGTAGCTGTATGTGTAGACTTAATGAGGAGAAAGTTCACCATTTACGACTCGATGCTATCAGCAAATCGGGATGAGTTCATCTGTGACCTACTGAATCCCATTGCCACAATGTTTCCATCATTGCTTATCCAGTCGGGGTTTTATAAAATGAGGCCTGAGTTGTCCCCTCTGAATACACCGTTTCAGGTTGTACGTCTAGCAGAAAATATCCCACAACAATCGGAAAGCGGAGATTGCGGAGTGTTCGTGCTTAAATTTGTGGAGTATTTGAGCTCCGGGCAGCAATTAAGTTTCAGACAAGAAGACATACCGGCATTTAGGGAGCGAATGGCCTTCCTAATGTTTGCCATGAGTCATGACATTGGTGGCAACATAAATACACTTGTGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

451

Amino Acids

52.03

Weight (kDa)

5.01

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 232 - 438 8.1e-38 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021650)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002194.1_g000018
rosa_roxburghii Rroxscaffold_1G00027280
rosa_samantha Rh5BG360600 Rh5DG375400
rosa_wichuraiana Rw5G032970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1107
AccB7I CCANNNNNTGG 1 cut(s) 623
AccI GTMKAC 2 cut(s) 432, 978
AccII CGCG 1 cut(s) 633
AciI CCGC 4 cut(s) 74, 210, 1191, 1200
AclI AACGTT 1 cut(s) 945
AclWI GGATC 3 cut(s) 127, 614, 772
AcsI RAATTY 2 cut(s) 197, 1217
AcuI CTGAAG 3 cut(s) 119, 276, 552
AfaI GTAC 3 cut(s) 728, 932, 1155
AfiI CCNNNNNNNGG 7 cut(s) 209, 316, 623, 795, 852, 909, 961
AgsI TTSAA 4 cut(s) 154, 197, 541, 560
AjnI CCWGG 3 cut(s) 115, 685, 780
AloI GAACNNNNNNTCC 2 cut(s) 546, 578
AluBI AGCT 5 cut(s) 142, 296, 368, 968, 1236
AluI AGCT 5 cut(s) 142, 296, 368, 968, 1236
Alw21I GWGCWC 1 cut(s) 1238
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 3 cut(s) 127, 614, 772
AoxI GGCC 4 cut(s) 113, 460, 1115, 1293
ApaI GGGCCC 1 cut(s) 464
ApeKI GCWGC 1 cut(s) 1243
ApoI RAATTY 2 cut(s) 197, 1217
Asp700I GAANNNNTTC 2 cut(s) 60, 129
AspLEI GCGC 2 cut(s) 471, 633
AspS9I GGNCC 3 cut(s) 256, 460, 461
AsuC2I CCSGG 3 cut(s) 204, 464, 1240
AsuHPI GGTGA 5 cut(s) 193, 271, 892, 952, 991
AsuII TTCGAA 1 cut(s) 648
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 464
BanII GRGCYC 2 cut(s) 464, 1238
BbsI GAAGAC 3 cut(s) 540, 678, 1272
Bbv12I GWGCWC 1 cut(s) 1238
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 1255
BccI CCATC 7 cut(s) 310, 328, 404, 533, 617, 810, 1087
BceAI ACGGC 2 cut(s) 290, 644
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 3 cut(s) 117, 687, 782
BciVI GTATCC 1 cut(s) 778
BcnI CCSGG 3 cut(s) 204, 464, 1240
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 4 cut(s) 53, 407, 566, 1160
BfmI CTRYAG 1 cut(s) 303
BfuI GTATCC 1 cut(s) 778
BisI GCNGC 2 cut(s) 211, 1244
BlsI GCNGC 2 cut(s) 212, 1245
Bme1390I CCNGG 6 cut(s) 117, 204, 464, 687, 782, 1240
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 3 cut(s) 256, 460, 461
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 6 cut(s) 117, 204, 464, 687, 782, 1240
BmrI ACTGGG 1 cut(s) 970
BmsI GCATC 6 cut(s) 197, 423, 511, 662, 706, 1005
BmuI ACTGGG 1 cut(s) 970
BpiI GAAGAC 3 cut(s) 540, 678, 1272
Bpu10I CCTNAGC 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 3 cut(s) 204, 464, 1240
BsaAI YACGTR 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 124
BsaJI CCNNGG 3 cut(s) 311, 685, 880
BsaWI WCCGGW 1 cut(s) 937
BsaXI ACNNNNNCTCC 4 cut(s) 827, 857, 1194, 1224
Bsc4I CCNNNNNNNGG 7 cut(s) 209, 316, 623, 795, 852, 909, 961
Bse118I RCCGGY 1 cut(s) 1273
Bse1I ACTGG 2 cut(s) 965, 1093
Bse3DI GCAATG 2 cut(s) 1062, 1082
Bse8I GATNNNNATC 1 cut(s) 124
BseBI CCWGG 3 cut(s) 117, 687, 782
BseDI CCNNGG 3 cut(s) 311, 685, 880
BseGI GGATG 6 cut(s) 109, 212, 321, 613, 810, 1039
BseJI GATNNNNATC 1 cut(s) 124
BseLI CCNNNNNNNGG 7 cut(s) 209, 316, 623, 795, 852, 909, 961
BseMI GCAATG 2 cut(s) 1062, 1082
BseMII CTCAG 2 cut(s) 360, 1110
BseNI ACTGG 2 cut(s) 965, 1093
BseRI GAGGAG 2 cut(s) 337, 1003
BseSI GKGCMC 1 cut(s) 464
BseXI GCAGC 1 cut(s) 1255
BsgI GTGCAG 2 cut(s) 60, 395
Bsh1236I CGCG 1 cut(s) 633
BshFI GGCC 4 cut(s) 115, 462, 1117, 1295
BsiHKAI GWGCWC 1 cut(s) 1238
BsiSI CCGG 5 cut(s) 204, 464, 938, 1239, 1274
BslFI GGGAC 2 cut(s) 293, 1111
BslI CCNNNNNNNGG 7 cut(s) 209, 316, 623, 795, 852, 909, 961
BsmAI GTCTC 1 cut(s) 384
BsmFI GGGAC 2 cut(s) 293, 1111
BsnI GGCC 4 cut(s) 115, 462, 1117, 1295
Bsp119I TTCGAA 1 cut(s) 648
Bsp120I GGGCCC 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 464, 1238
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 3 cut(s) 119, 606, 777
BspACI CCGC 4 cut(s) 74, 210, 1191, 1200
BspANI GGCC 4 cut(s) 115, 462, 1117, 1295
BspCNI CTCAG 2 cut(s) 361, 1111
BspFNI CGCG 1 cut(s) 633
BspHI TCATGA 1 cut(s) 1318
BspLI GGNNCC 1 cut(s) 462
BspPI GGATC 3 cut(s) 127, 614, 772
BspT104I TTCGAA 1 cut(s) 648
BsrDI GCAATG 2 cut(s) 1062, 1082
BsrFI RCCGGY 1 cut(s) 1273
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 2 cut(s) 965, 1093
BssAI RCCGGY 1 cut(s) 1273
BssECI CCNNGG 3 cut(s) 311, 685, 880
BssMI GATC 3 cut(s) 119, 606, 777
BssT1I CCWWGG 2 cut(s) 311, 880
Bst2UI CCWGG 3 cut(s) 117, 687, 782
Bst4CI ACNGT 3 cut(s) 790, 926, 1143
Bst6I CTCTTC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 930
BstBAI YACGTR 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 648
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 5 cut(s) 65, 369, 637, 1119, 1353
BstEII GGTNACC 1 cut(s) 181
BstF5I GGATG 6 cut(s) 109, 212, 321, 613, 810, 1039
BstFNI CGCG 1 cut(s) 633
BstHHI GCGC 2 cut(s) 471, 633
BstKTI GATC 3 cut(s) 122, 609, 780
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 3 cut(s) 119, 606, 777
BstMWI GCNNNNNNNGC 2 cut(s) 468, 1197
BstNI CCWGG 3 cut(s) 117, 687, 782
BstNSI RCATGY 1 cut(s) 713
BstPI GGTNACC 1 cut(s) 181
BstSCI CCNGG 6 cut(s) 115, 202, 462, 685, 780, 1238
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 464
BstUI CGCG 1 cut(s) 633
BstV1I GCAGC 1 cut(s) 1255
BstV2I GAAGAC 3 cut(s) 540, 678, 1272
BstXI CCANNNNNNTGG 1 cut(s) 693
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 4 cut(s) 115, 462, 1117, 1295
BtgZI GCGATG 2 cut(s) 347, 829
BtsCI GGATG 6 cut(s) 109, 212, 321, 613, 810, 1039
BtsIMutI CAGTG 2 cut(s) 528, 958
Cac8I GCNNGC 1 cut(s) 378
CciI TCATGA 1 cut(s) 1318
CfoI GCGC 2 cut(s) 471, 633
Cfr10I RCCGGY 1 cut(s) 1273
Cfr13I GGNCC 3 cut(s) 256, 460, 461
CseI GACGC 1 cut(s) 516
Csp6I GTAC 3 cut(s) 727, 931, 1154
CviAII CATG 6 cut(s) 511, 603, 710, 775, 1312, 1319
CviQI GTAC 3 cut(s) 727, 931, 1154
DdeI CTNAG 5 cut(s) 65, 369, 637, 1119, 1353
DpnI GATC 3 cut(s) 121, 608, 779
DpnII GATC 3 cut(s) 119, 606, 777
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 1236
Eco130I CCWWGG 2 cut(s) 311, 880
Eco147I AGGCCT 1 cut(s) 1117
Eco24I GRGCYC 2 cut(s) 464, 1238
Eco47I GGWCC 1 cut(s) 256
Eco53kI GAGCTC 1 cut(s) 1236
Eco57I CTGAAG 3 cut(s) 119, 276, 552
Eco91I GGTNACC 1 cut(s) 181
EcoICRI GAGCTC 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 460
EcoO65I GGTNACC 1 cut(s) 181
EcoRII CCWGG 3 cut(s) 115, 685, 780
EcoT14I CCWWGG 2 cut(s) 311, 880
EcoT38I GRGCYC 2 cut(s) 464, 1238
ErhI CCWWGG 2 cut(s) 311, 880
FaeI CATG 6 cut(s) 514, 606, 713, 778, 1315, 1322
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 2 cut(s) 293, 1111
FatI CATG 6 cut(s) 510, 602, 709, 774, 1311, 1318
FauI CCCGC 1 cut(s) 67
FblI GTMKAC 2 cut(s) 432, 978
Fnu4HI GCNGC 2 cut(s) 211, 1244
FokI GGATG 6 cut(s) 116, 219, 328, 600, 817, 1046
FriOI GRGCYC 2 cut(s) 464, 1238
Fsp4HI GCNGC 2 cut(s) 211, 1244
FspBI CTAG 4 cut(s) 53, 407, 566, 1160
GlaI GCGC 2 cut(s) 470, 632
GluI GCNGC 2 cut(s) 211, 1244
HaeIII GGCC 4 cut(s) 115, 462, 1117, 1295
HapII CCGG 5 cut(s) 204, 464, 938, 1239, 1274
HgaI GACGC 1 cut(s) 516
HhaI GCGC 2 cut(s) 471, 633
Hin1II CATG 6 cut(s) 514, 606, 713, 778, 1315, 1322
Hin6I GCGC 2 cut(s) 469, 631
HinP1I GCGC 2 cut(s) 469, 631
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 5 cut(s) 125, 857, 1010, 1057, 1315
HpaII CCGG 5 cut(s) 204, 464, 938, 1239, 1274
HphI GGTGA 5 cut(s) 193, 271, 892, 952, 991
Hpy166II GTNNAC 6 cut(s) 404, 433, 922, 943, 979, 999
Hpy188I TCNGA 9 cut(s) 10, 124, 138, 270, 288, 862, 1134, 1186, 1259
Hpy188III TCNNGA 6 cut(s) 25, 189, 617, 829, 1031, 1319
Hpy8I GTNNAC 6 cut(s) 404, 433, 922, 943, 979, 999
HpyAV CCTTC 2 cut(s) 451, 1306
HpyCH4III ACNGT 3 cut(s) 790, 926, 1143
HpyCH4IV ACGT 4 cut(s) 352, 516, 945, 1156
HpyCH4V TGCA 3 cut(s) 41, 376, 774
HpyF10VI GCNNNNNNNGC 2 cut(s) 468, 1197
HpyF3I CTNAG 5 cut(s) 65, 369, 637, 1119, 1353
HpySE526I ACGT 4 cut(s) 352, 516, 945, 1156
Hsp92II CATG 6 cut(s) 514, 606, 713, 778, 1315, 1322
HspAI GCGC 2 cut(s) 469, 631
Kzo9I GATC 3 cut(s) 119, 606, 777
LmnI GCTCC 3 cut(s) 466, 1241, 1285
Lsp1109I GCAGC 1 cut(s) 1255
LweI GCATC 6 cut(s) 197, 423, 511, 662, 706, 1005
MaeI CTAG 4 cut(s) 53, 407, 566, 1160
MaeII ACGT 4 cut(s) 352, 516, 945, 1156
MaeIII GTNAC 9 cut(s) 181, 348, 487, 497, 544, 701, 946, 1046, 1348
MalI GATC 3 cut(s) 121, 608, 779
MboI GATC 3 cut(s) 119, 606, 777
MboII GAAGA 6 cut(s) 122, 125, 545, 637, 683, 1277
MhlI GDGCHC 2 cut(s) 464, 1238
MluCI AATT 7 cut(s) 149, 166, 197, 480, 896, 1217, 1247
MlyI GAGTC 2 cut(s) 1004, 1324
MmeI TCCRAC 3 cut(s) 311, 648, 782
MroXI GAANNNNTTC 2 cut(s) 60, 129
MseI TTAA 4 cut(s) 363, 983, 1215, 1250
MslI CAYNNNNRTG 2 cut(s) 714, 938
MspI CCGG 5 cut(s) 204, 464, 938, 1239, 1274
MspR9I CCNGG 6 cut(s) 117, 204, 464, 687, 782, 1240
MvaI CCWGG 3 cut(s) 117, 687, 782
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 2 cut(s) 468, 1197
NciI CCSGG 3 cut(s) 204, 464, 1240
NdeII GATC 3 cut(s) 119, 606, 777
NlaIII CATG 6 cut(s) 514, 606, 713, 778, 1315, 1322
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 4 cut(s) 181, 701, 1046, 1348
NspI RCATGY 1 cut(s) 713
NspV TTCGAA 1 cut(s) 648
PagI TCATGA 1 cut(s) 1318
PceI AGGCCT 1 cut(s) 1117
PdmI GAANNNNTTC 2 cut(s) 60, 129
PfeI GAWTC 3 cut(s) 125, 857, 1057
PflMI CCANNNNNTGG 1 cut(s) 623
PfoI TCCNGGA 2 cut(s) 202, 780
PkrI GCNGC 2 cut(s) 212, 1245
PleI GAGTC 2 cut(s) 1004, 1323
PpsI GAGTC 2 cut(s) 1004, 1323
Ppu21I YACGTR 1 cut(s) 353
PsiI TTATAA 1 cut(s) 1107
Psp124BI GAGCTC 1 cut(s) 1238
Psp1406I AACGTT 1 cut(s) 945
Psp6I CCWGG 3 cut(s) 115, 685, 780
PspEI GGTNACC 1 cut(s) 181
PspGI CCWGG 3 cut(s) 115, 685, 780
PspN4I GGNNCC 1 cut(s) 462
PspOMI GGGCCC 1 cut(s) 460
PspPI GGNCC 3 cut(s) 256, 460, 461
RsaI GTAC 3 cut(s) 728, 932, 1155
RsaNI GTAC 3 cut(s) 727, 931, 1154
RseI CAYNNNNRTG 2 cut(s) 714, 938
SacI GAGCTC 1 cut(s) 1238
SaqAI TTAA 4 cut(s) 363, 983, 1215, 1250
SatI GCNGC 2 cut(s) 211, 1244
Sau3AI GATC 3 cut(s) 119, 606, 777
Sau96I GGNCC 3 cut(s) 256, 460, 461
SchI GAGTC 2 cut(s) 1004, 1324
ScrFI CCNGG 6 cut(s) 117, 204, 464, 687, 782, 1240
SduI GDGCHC 2 cut(s) 464, 1238
SfaNI GCATC 6 cut(s) 197, 423, 511, 662, 706, 1005
SfcI CTRYAG 1 cut(s) 303
SfuI TTCGAA 1 cut(s) 648
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 2 cut(s) 714, 938
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
Sse9I AATT 7 cut(s) 149, 166, 197, 480, 896, 1217, 1247
SseBI AGGCCT 1 cut(s) 1117
SsiI CCGC 4 cut(s) 74, 210, 1191, 1200
SspMI CTAG 4 cut(s) 53, 407, 566, 1160
SstI GAGCTC 1 cut(s) 1238
StuI AGGCCT 1 cut(s) 1117
StyD4I CCNGG 6 cut(s) 115, 202, 462, 685, 780, 1238
StyI CCWWGG 2 cut(s) 311, 880
TaaI ACNGT 3 cut(s) 790, 926, 1143
TaiI ACGT 4 cut(s) 355, 519, 948, 1159
TaqI TCGA 2 cut(s) 648, 1013
TaqII GACCGA 1 cut(s) 599
TasI AATT 7 cut(s) 149, 166, 197, 480, 896, 1217, 1247
TatI WGTACW 2 cut(s) 726, 930
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 3 cut(s) 125, 857, 1057
Tru1I TTAA 4 cut(s) 363, 983, 1215, 1250
Tru9I TTAA 4 cut(s) 363, 983, 1215, 1250
TscAI CASTG 2 cut(s) 535, 965
TseFI GTSAC 4 cut(s) 181, 701, 1046, 1348
TseI GCWGC 1 cut(s) 1243
Tsp45I GTSAC 4 cut(s) 181, 701, 1046, 1348
TspDTI ATGAA 2 cut(s) 162, 1030
TspRI CASTG 2 cut(s) 535, 965
Van91I CCANNNNNTGG 1 cut(s) 623
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 2 cut(s) 197, 1217
XceI RCATGY 1 cut(s) 713
XmiI GTMKAC 2 cut(s) 432, 978
XmnI GAANNNNTTC 2 cut(s) 60, 129
XspI CTAG 4 cut(s) 53, 407, 566, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.