Rmu_sc0002354.1_g000054

CRAL-TRIO domain-containing protein C23B6.04c-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002354.1
OG0020043
Physical Location & Seq
Reverse (-)
261069 .. 261558
490 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
atgtcatggttgattgacttcactggcttctcactgaacaccaatgtttccgtcaagacagctcgtgaatgtatcaacattctacagaaccattaccccgagaggcttgcagttgcatttctttacaacccacctaggatttttcaggcattctggaagacgcacgtacgcccactcgtcgtcgtcgtttcatccaacacctctcctgcacaatcccaactcatccgatcggccaagacccagaagctctacgacatagttgagctcccagcaccctccggccacgactccaacgccgccggttcagtgttcacgcacctctcggccatcatgttcgatgcccgatcatctctccggtctgcaatctcggccatggagctcaaacccaccgccatgatcgtcgattctagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

137

Amino Acids

15.21

Weight (kDa)

9.57

Isoelectric Point (pI)

58.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 3 - 54 1.1e-12 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020043)

Species Orthologous Gene IDs
rosa_laevigata RLG00000033650
rosa_multiflora Rmu_sc0002354.1_g000054 Rmu_sc0004948.1_g000002 Rmu_sc0012562.1_g000005
rosa_samantha Rh2AG196700 Rh2DG204600
rosa_wichuraiana Rw1G000800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 297, 390
AcoI YGGCCR 4 cut(s) 231, 280, 324, 369
AfaI GTAC 1 cut(s) 168
AluBI AGCT 4 cut(s) 62, 247, 265, 379
AluI AGCT 4 cut(s) 62, 247, 265, 379
Alw21I GWGCWC 2 cut(s) 267, 381
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 4 cut(s) 231, 280, 324, 369
AspA2I CCTAGG 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 98
AvrII CCTAGG 1 cut(s) 134
BanII GRGCYC 2 cut(s) 267, 381
BauI CACGAG 1 cut(s) 63
BbsI GAAGAC 1 cut(s) 164
Bbv12I GWGCWC 2 cut(s) 267, 381
BccI CCATC 1 cut(s) 335
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BfaI CTAG 2 cut(s) 135, 408
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 1 cut(s) 297
BlnI CCTAGG 1 cut(s) 134
BlsI GCNGC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 98
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 134, 372
BsaWI WCCGGW 1 cut(s) 354
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 28
BseDI CCNNGG 2 cut(s) 134, 372
BseGI GGATG 2 cut(s) 191, 222
BseNI ACTGG 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 268
BsgI GTGCAG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 4 cut(s) 233, 282, 326, 371
BsiEI CGRYCG 1 cut(s) 230
BsiHKAI GWGCWC 2 cut(s) 267, 381
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 3 cut(s) 279, 300, 355
BsiWI CGTACG 1 cut(s) 166
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 4 cut(s) 233, 282, 326, 371
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 267, 381
Bsp143I GATC 3 cut(s) 227, 344, 396
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 2 cut(s) 297, 390
BspANI GGCC 4 cut(s) 233, 282, 326, 371
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 2 cut(s) 134, 372
BssMI GATC 3 cut(s) 227, 344, 396
BssSI CACGAG 1 cut(s) 63
BssT1I CCWWGG 2 cut(s) 134, 372
Bst2BI CACGAG 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 108
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 191, 222
BstKTI GATC 3 cut(s) 230, 347, 399
BstMBI GATC 3 cut(s) 227, 344, 396
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstSFI CTRYAG 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 164
BsuRI GGCC 4 cut(s) 233, 282, 326, 371
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 191, 222
BtsIMutI CAGTG 3 cut(s) 21, 32, 312
Cac8I GCNNGC 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 299
CseI GACGC 1 cut(s) 169
Csp6I GTAC 1 cut(s) 167
CviAII CATG 4 cut(s) 6, 331, 373, 394
CviQI GTAC 1 cut(s) 167
DpnI GATC 3 cut(s) 229, 346, 398
DpnII GATC 3 cut(s) 227, 344, 396
EaeI YGGCCR 4 cut(s) 231, 280, 324, 369
Ecl136II GAGCTC 2 cut(s) 265, 379
Eco130I CCWWGG 2 cut(s) 134, 372
Eco24I GRGCYC 2 cut(s) 267, 381
Eco53kI GAGCTC 2 cut(s) 265, 379
Eco88I CYCGRG 1 cut(s) 98
EcoICRI GAGCTC 2 cut(s) 265, 379
EcoT14I CCWWGG 2 cut(s) 134, 372
EcoT38I GRGCYC 2 cut(s) 267, 381
ErhI CCWWGG 2 cut(s) 134, 372
FaeI CATG 4 cut(s) 9, 334, 376, 397
FaiI YATR 5 cut(s) 7, 257, 332, 374, 395
FatI CATG 4 cut(s) 5, 330, 372, 393
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 2 cut(s) 178, 209
FriOI GRGCYC 2 cut(s) 267, 381
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 2 cut(s) 135, 408
GluI GCNGC 1 cut(s) 297
GsaI CCCAGC 1 cut(s) 272
HaeIII GGCC 4 cut(s) 233, 282, 326, 371
HapII CCGG 3 cut(s) 279, 300, 355
HgaI GACGC 1 cut(s) 169
Hin1II CATG 4 cut(s) 9, 334, 376, 397
HinfI GANTC 2 cut(s) 287, 404
HpaII CCGG 3 cut(s) 279, 300, 355
Hpy166II GTNNAC 1 cut(s) 312
Hpy188I TCNGA 1 cut(s) 227
Hpy188III TCNNGA 4 cut(s) 55, 65, 154, 408
Hpy8I GTNNAC 1 cut(s) 312
Hpy99I CGWCG 4 cut(s) 182, 185, 188, 404
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 4 cut(s) 110, 116, 209, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 4 cut(s) 9, 334, 376, 397
Kzo9I GATC 3 cut(s) 227, 344, 396
LmnI GCTCC 2 cut(s) 270, 376
LpnPI CCDG 9 cut(s) 9, 131, 139, 219, 254, 282, 292, 313, 368
LweI GCATC 1 cut(s) 328
MaeI CTAG 2 cut(s) 135, 408
MaeII ACGT 1 cut(s) 165
MalI GATC 3 cut(s) 229, 346, 398
MboI GATC 3 cut(s) 227, 344, 396
MboII GAAGA 1 cut(s) 169
MhlI GDGCHC 2 cut(s) 267, 381
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 2 cut(s) 219, 315
MnlI CCTC 4 cut(s) 96, 211, 286, 329
MslI CAYNNNNRTG 1 cut(s) 392
MspI CCGG 3 cut(s) 279, 300, 355
Mva1269I GAATGC 1 cut(s) 149
MwoI GCNNNNNNNGC 1 cut(s) 368
NcoI CCATGG 1 cut(s) 372
NdeII GATC 3 cut(s) 227, 344, 396
NlaIII CATG 4 cut(s) 9, 334, 376, 397
NmeAIII GCCGAG 2 cut(s) 302, 347
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 404
Pfl23II CGTACG 1 cut(s) 166
PkrI GCNGC 1 cut(s) 298
Ple19I CGATCG 1 cut(s) 230
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
Ppu21I YACGTR 1 cut(s) 166
Psp124BI GAGCTC 2 cut(s) 267, 381
PspFI CCCAGC 1 cut(s) 268
PspLI CGTACG 1 cut(s) 166
PvuI CGATCG 1 cut(s) 230
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 2 cut(s) 267, 381
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 3 cut(s) 227, 344, 396
SchI GAGTC 1 cut(s) 281
SduI GDGCHC 2 cut(s) 267, 381
SetI ASST 8 cut(s) 64, 136, 168, 203, 249, 267, 321, 381
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 392
SsiI CCGC 2 cut(s) 297, 390
SspMI CTAG 2 cut(s) 135, 408
SstI GAGCTC 2 cut(s) 267, 381
StyI CCWWGG 2 cut(s) 134, 372
TaiI ACGT 1 cut(s) 168
TaqI TCGA 2 cut(s) 336, 402
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 1 cut(s) 404
TscAI CASTG 3 cut(s) 28, 39, 312
TspDTI ATGAA 1 cut(s) 180
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 3 cut(s) 28, 39, 312
XbaI TCTAGA 1 cut(s) 407
XmaJI CCTAGG 1 cut(s) 134
XspI CTAG 2 cut(s) 135, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.