Rw1G000800

CRAL-TRIO domain-containing protein C23B6.04c-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0020043
Physical Location & Seq
Reverse (-)
1138741 .. 1140318
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000800.1

Sequence Viewer

Length: 489 bp
ATGAAGAACACAAATTCACCGGAAGGCAATGTTCGCCATTTAGTCTATCTTATAGAAAATGGTATCCTCAACCTTCGTGAAGGTCAAGAACAGATGTCATGGTTGATTGACTTCACTGGCTTCTCACTGAACACCAATGTTTCCGTCAAGACAGCTCGTGAATGTATCAACATTCTACAGAACCATTACCCTGAGAGGCTTGCAGTTGCATTTCTTTACAACCCACCTAGGATTTTTCAGGCATTCTGGAAGTTGCCTACAAAAGAGTTCCACCTGAAAATAAATGCAATCTATGACTTTAAGAAATCTATAAATACTGGCTCAATAAAGTCAGCAAGAATGCAAATATGTCACCATGAATCACAAAGGTGTACACCGAGTTTATGTATCCACCAATGCATGCAAGATGAAATAACAGGGCAAGCTGGACAAATATTTTTAGGCTCAAATTCAGTGGCGGATCCAGGATACAAAAGTCGTCTAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

162

Amino Acids

18.47

Weight (kDa)

8.85

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 6 - 85 3.2e-19 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020043)

Species Orthologous Gene IDs
rosa_laevigata RLG00000033650
rosa_multiflora Rmu_sc0002354.1_g000054 Rmu_sc0004948.1_g000002 Rmu_sc0012562.1_g000005
rosa_samantha Rh2AG196700 Rh2DG204600
rosa_wichuraiana Rw1G000800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 458
AclWI GGATC 2 cut(s) 455, 468
AcsI RAATTY 2 cut(s) 13, 448
AfaI GTAC 1 cut(s) 373
AjnI CCWGG 1 cut(s) 463
AleI CACNNNNGTG 1 cut(s) 367
AluBI AGCT 2 cut(s) 155, 425
AluI AGCT 2 cut(s) 155, 425
AlwI GGATC 2 cut(s) 455, 468
ApoI RAATTY 2 cut(s) 13, 448
AspA2I CCTAGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 9, 344
AvrII CCTAGG 1 cut(s) 227
BaeI ACNNNNGTAYC 1 cut(s) 460
BamHI GGATCC 1 cut(s) 460
BauI CACGAG 1 cut(s) 156
BciT130I CCWGG 1 cut(s) 465
BciVI GTATCC 3 cut(s) 74, 398, 461
BfaI CTAG 2 cut(s) 228, 482
BfmI CTRYAG 1 cut(s) 176
BfuI GTATCC 3 cut(s) 74, 398, 461
BlnI CCTAGG 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 465
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 227
BsaWI WCCGGW 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 121, 322
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 227
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 2 cut(s) 121, 322
BsiSI CCGG 1 cut(s) 20
BsmI GAATGC 2 cut(s) 242, 345
Bsp1407I TGTACA 1 cut(s) 371
Bsp143I GATC 1 cut(s) 460
BspACI CCGC 1 cut(s) 458
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 462
BspPI GGATC 2 cut(s) 455, 468
BsrDI GCAATG 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 371
BsrI ACTGG 2 cut(s) 121, 322
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 1 cut(s) 460
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 465
BstAUI TGTACA 1 cut(s) 371
BstC8I GCNNGC 3 cut(s) 201, 401, 423
BstDEI CTNAG 1 cut(s) 192
BstKTI GATC 1 cut(s) 463
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 463
BstSFI CTRYAG 1 cut(s) 176
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BsuI GTATCC 3 cut(s) 74, 398, 461
BtsIMutI CAGTG 3 cut(s) 114, 125, 459
Cac8I GCNNGC 3 cut(s) 201, 401, 423
Csp6I GTAC 1 cut(s) 372
CspCI CAANNNNNGTGG 2 cut(s) 435, 470
CviAII CATG 3 cut(s) 99, 356, 400
CviJI RGCY 7 cut(s) 120, 155, 199, 321, 425, 444, 486
CviKI_1 RGCY 7 cut(s) 120, 155, 199, 321, 425, 444, 486
CviQI GTAC 1 cut(s) 372
DdeI CTNAG 1 cut(s) 192
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
EciI GGCGGA 1 cut(s) 473
Eco130I CCWWGG 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 227
EcoT22I ATGCAT 1 cut(s) 401
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 3 cut(s) 102, 359, 403
FaiI YATR 8 cut(s) 53, 100, 294, 311, 349, 357, 385, 401
FatI CATG 3 cut(s) 98, 355, 399
FspBI CTAG 2 cut(s) 228, 482
HapII CCGG 1 cut(s) 20
Hin1II CATG 3 cut(s) 102, 359, 403
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 9, 344
Hpy166II GTNNAC 2 cut(s) 372, 374
Hpy188III TCNNGA 5 cut(s) 77, 86, 148, 158, 247
Hpy8I GTNNAC 2 cut(s) 372, 374
HpyAV CCTTC 3 cut(s) 17, 74, 83
HpyCH4V TGCA 6 cut(s) 203, 209, 287, 343, 399, 403
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 1 cut(s) 192
Hsp92II CATG 3 cut(s) 102, 359, 403
Kzo9I GATC 1 cut(s) 460
MaeI CTAG 2 cut(s) 228, 482
MaeIII GTNAC 1 cut(s) 350
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 1 cut(s) 16
MflI RGATCY 1 cut(s) 460
MluCI AATT 2 cut(s) 13, 448
MnlI CCTC 2 cut(s) 77, 189
Mph1103I ATGCAT 1 cut(s) 401
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 367
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 465
Mva1269I GAATGC 2 cut(s) 242, 345
MvaI CCWGG 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 33
NdeII GATC 1 cut(s) 460
NlaIII CATG 3 cut(s) 102, 359, 403
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 1 cut(s) 350
NsiI ATGCAT 1 cut(s) 401
NspI RCATGY 1 cut(s) 403
OliI CACNNNNGTG 1 cut(s) 367
PaeI GCATGC 1 cut(s) 403
PctI GAATGC 2 cut(s) 242, 345
PfeI GAWTC 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 462
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 1 cut(s) 373
RsaNI GTAC 1 cut(s) 372
RseI CAYNNNNRTG 1 cut(s) 367
SaqAI TTAA 1 cut(s) 300
Sau3AI GATC 1 cut(s) 460
ScrFI CCNGG 1 cut(s) 465
SetI ASST 7 cut(s) 75, 85, 157, 229, 276, 371, 427
SfcI CTRYAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 367
SphI GCATGC 1 cut(s) 403
Sse9I AATT 2 cut(s) 13, 448
SsiI CCGC 1 cut(s) 458
SspI AATATT 1 cut(s) 435
SspMI CTAG 2 cut(s) 228, 482
StyD4I CCNGG 1 cut(s) 463
StyI CCWWGG 1 cut(s) 227
TasI AATT 2 cut(s) 13, 448
TatI WGTACW 1 cut(s) 371
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 3 cut(s) 121, 132, 459
TseFI GTSAC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 3 cut(s) 17, 372, 423
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 3 cut(s) 121, 132, 459
XapI RAATTY 2 cut(s) 13, 448
XceI RCATGY 1 cut(s) 403
XmaJI CCTAGG 1 cut(s) 227
XspI CTAG 2 cut(s) 228, 482
Zsp2I ATGCAT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.