Rmu_sc0002776.1_g000036

Protein MID1-COMPLEMENTING ACTIVITY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002776.1
OG0019889
Physical Location & Seq
Reverse (-)
167417 .. 167854
438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002776.1_g000036.1.cds

Sequence Viewer

Length: 438 bp
atggcgtcatgggaccacttcggggatattgccaatgtggctcagttgacggggctggacgccgtgaagctgatcggaatgatcgtgaaggcggcggccacggcgagaatgcacaagaagaactgcaggcaatttgcgcagcatctgaagctgatcgggaatttattggagcaactcaagatctcggagctcaagaggtatccggagactcgagagccgttggagcagctggaggaggctctgaggaggtcatacattttggtcaacagctgccaagaccggagctatttgtatctgcttgcaatgggatggaacatcgtgtaccagttcaggaaggctcagaatgaaattgatagatacttgaagctcctccacgaattcttgtctggccattgttatgtgtcagcggatgggtatgtaatgaccttcttggcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

145

Amino Acids

16.78

Weight (kDa)

8.76

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019889)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105791
rosa_multiflora Rmu_sc0001361.1_g000015 Rmu_sc0002776.1_g000036
rosa_roxburghii Rroxscaffold_1G00036870 Rroxscaffold_7G00185670
rosa_samantha Rh5AG012200 Rh5BG015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 138
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 3 cut(s) 92, 95, 407
AcoI YGGCCR 2 cut(s) 96, 388
AcsI RAATTY 2 cut(s) 160, 377
AcuI CTGAAG 1 cut(s) 167
AcyI GRCGYC 2 cut(s) 5, 60
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 1 cut(s) 364
AluBI AGCT 7 cut(s) 70, 151, 190, 229, 270, 285, 367
AluI AGCT 7 cut(s) 70, 151, 190, 229, 270, 285, 367
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 1 cut(s) 200
AlwNI CAGNNNCTG 1 cut(s) 145
Ama87I CYCGRG 1 cut(s) 210
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 2 cut(s) 96, 388
ApeKI GCWGC 3 cut(s) 139, 226, 270
ApoI RAATTY 2 cut(s) 160, 377
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 1 cut(s) 13
BalI TGGCCA 1 cut(s) 390
BanII GRGCYC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 151, 238, 257
BccI CCATC 2 cut(s) 303, 404
BceAI ACGGC 3 cut(s) 47, 117, 202
BciVI GTATCC 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 124
BfuI GTATCC 1 cut(s) 210
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 1 cut(s) 180
BisI GCNGC 5 cut(s) 93, 96, 140, 227, 271
BlsI GCNGC 5 cut(s) 94, 97, 141, 228, 272
Bme18I GGWCC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 14
BmsI GCATC 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 251
BpuEI CTTGAG 2 cut(s) 161, 176
BsaHI GRCGYC 2 cut(s) 5, 60
BsaJI CCNNGG 1 cut(s) 99
BsaWI WCCGGW 2 cut(s) 202, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 325
Bse3DI GCAATG 1 cut(s) 309
BseAI TCCGGA 1 cut(s) 202
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 2 cut(s) 314, 415
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 3 cut(s) 56, 233, 353
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 3 cut(s) 248, 259, 359
BseXI GCAGC 3 cut(s) 151, 238, 257
BshFI GGCC 2 cut(s) 98, 390
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 2 cut(s) 203, 280
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 114
BsnI GGCC 2 cut(s) 98, 390
BsoBI CYCGRG 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 4 cut(s) 72, 81, 153, 180
BspACI CCGC 3 cut(s) 92, 95, 407
BspANI GGCC 2 cut(s) 98, 390
BspCNI CTCAG 3 cut(s) 55, 234, 352
BspEI TCCGGA 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 14
BspMAI CTGCAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 4 cut(s) 72, 81, 153, 180
BssNI GRCGYC 2 cut(s) 5, 60
BstACI GRCGYC 2 cut(s) 5, 60
BstC8I GCNNGC 2 cut(s) 128, 300
BstDEI CTNAG 3 cut(s) 42, 242, 339
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 314, 415
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 4 cut(s) 75, 84, 156, 183
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 4 cut(s) 72, 81, 153, 180
BstMWI GCNNNNNNNGC 5 cut(s) 38, 101, 136, 148, 223
BstSFI CTRYAG 1 cut(s) 124
BstV1I GCAGC 3 cut(s) 151, 238, 257
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 98, 390
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 2 cut(s) 314, 415
Cac8I GCNNGC 2 cut(s) 128, 300
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 13
CseI GACGC 1 cut(s) 68
Csp6I GTAC 1 cut(s) 322
CviAII CATG 2 cut(s) 9, 435
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 3 cut(s) 42, 242, 339
DpnI GATC 4 cut(s) 74, 83, 155, 182
DpnII GATC 4 cut(s) 72, 81, 153, 180
EaeI YGGCCR 2 cut(s) 96, 388
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 13
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 2 cut(s) 12, 438
FaiI YATR 5 cut(s) 10, 253, 399, 417, 436
FaqI GGGAC 1 cut(s) 26
FatI CATG 2 cut(s) 8, 434
Fnu4HI GCNGC 5 cut(s) 93, 96, 140, 227, 271
FokI GGATG 2 cut(s) 321, 422
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 5 cut(s) 93, 96, 140, 227, 271
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 1 cut(s) 138
GluI GCNGC 5 cut(s) 93, 96, 140, 227, 271
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 2 cut(s) 98, 390
HapII CCGG 2 cut(s) 203, 280
HgaI GACGC 1 cut(s) 68
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 2 cut(s) 5, 60
Hin1II CATG 2 cut(s) 12, 438
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 2 cut(s) 48, 265
HindII GTYRAC 2 cut(s) 48, 265
HinfI GANTC 1 cut(s) 208
HpaII CCGG 2 cut(s) 203, 280
Hpy166II GTNNAC 3 cut(s) 48, 265, 322
Hpy188I TCNGA 5 cut(s) 77, 147, 187, 243, 342
Hpy188III TCNNGA 7 cut(s) 85, 157, 178, 193, 203, 212, 331
Hpy8I GTNNAC 3 cut(s) 48, 265, 322
HpyAV CCTTC 3 cut(s) 82, 328, 436
HpyCH4V TGCA 3 cut(s) 112, 126, 302
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 101, 136, 148, 223
HpyF3I CTNAG 3 cut(s) 42, 242, 339
Hsp92I GRCGYC 2 cut(s) 5, 60
Hsp92II CATG 2 cut(s) 12, 438
HspAI GCGC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 72, 81, 153, 180
LmnI GCTCC 5 cut(s) 169, 187, 223, 282, 372
LpnPI CCDG 8 cut(s) 41, 112, 215, 216, 293, 316, 338, 372
Lsp1109I GCAGC 3 cut(s) 151, 238, 257
LweI GCATC 1 cut(s) 151
MalI GATC 4 cut(s) 74, 83, 155, 182
MboI GATC 4 cut(s) 72, 81, 153, 180
MboII GAAGA 1 cut(s) 130
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 390
MluCI AATT 4 cut(s) 131, 160, 348, 377
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 1 cut(s) 202
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 6 cut(s) 189, 226, 229, 237, 240, 380
Mox20I TGGCCA 1 cut(s) 390
MroI TCCGGA 1 cut(s) 202
MscI TGGCCA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 396
Msp20I TGGCCA 1 cut(s) 390
MspA1I CMGCKG 3 cut(s) 229, 270, 407
MspI CCGG 2 cut(s) 203, 280
Mva1269I GAATGC 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 38, 101, 136, 148, 223
NdeII GATC 4 cut(s) 72, 81, 153, 180
NlaIII CATG 2 cut(s) 12, 438
NlaIV GGNNCC 1 cut(s) 14
NsbI TGCGCA 1 cut(s) 138
PaeR7I CTCGAG 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 114
PkrI GCNGC 5 cut(s) 94, 97, 141, 228, 272
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 1 cut(s) 13
PsrI GAACNNNNNNTAC 2 cut(s) 305, 337
PstI CTGCAG 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 180
PvuII CAGCTG 2 cut(s) 229, 270
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 396
SacI GAGCTC 1 cut(s) 192
SatI GCNGC 5 cut(s) 93, 96, 140, 227, 271
Sau3AI GATC 4 cut(s) 72, 81, 153, 180
Sau96I GGNCC 1 cut(s) 13
SchI GAGTC 1 cut(s) 202
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 210
SinI GGWCC 1 cut(s) 13
SlaI CTCGAG 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 3 cut(s) 176, 191, 210
SmoI CTYRAG 3 cut(s) 176, 191, 210
Sse9I AATT 4 cut(s) 131, 160, 348, 377
SsiI CCGC 3 cut(s) 92, 95, 407
SstI GAGCTC 1 cut(s) 192
TaqI TCGA 1 cut(s) 211
TasI AATT 4 cut(s) 131, 160, 348, 377
TauI GCSGC 2 cut(s) 95, 98
TseI GCWGC 3 cut(s) 139, 226, 270
TspDTI ATGAA 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 2 cut(s) 160, 377
XhoI CTCGAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.