Rroxscaffold_1G00036870

Protein MID1-COMPLEMENTING ACTIVITY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019889
Physical Location & Seq
Reverse (-)
53811141 .. 53826699
15559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00036870.1

Sequence Viewer

Length: 744 bp
ATGTCCACGATCTCCTGCAGCCCCATGATCCGACAGCTCACCTTCCTCCTTGCCTCCCTCACCGTGGGGTCCACCGGGGCACGGAGTCCAAACTCGGTAGCAACTTCATCGGCGGCTCGTTCATCCTCGGCTTCTCCGGCATGCGCTCCTCACGGCGTCGACCGTCTCCCCGCCGTTGGATCAGGCGCTGGAACTCGTCGGCCTCGGAATGGACGGAATCTTTTGGGGCGGTGCCACGGCGAGAATGCACAAGAAGAACTGCAGGCAAATTGCGCGGCATCCGAAGCCGATTGGGAATTTATTGGAGCAACTCAAGATCTCGGAGCTCAAGAGGTATCCGGAGACTCGAGAGCCGTTGGAGAGCCGGAGGAAGCTCGAGGAGGTCATACATTATGGTCAACGGTGCCGGGACCGGAGCTATTTGTATCGCTTGCAATGGGATGGAACATCGTGTACCGGTTCGGGAAGGCTCGGAATGAAATTGATAGATACTTGAAGCTTCCAATTCGATTTAGATTGAGAAACACTAACCCACCGTCATTCAATTTCGAATGGAGGTTCTTGGACTTCGTGGTGTGGAGTTTCGAAGGAGAGGAGCTACGGGGTAGCTTTGGTAGAAATCGAGAAATTGGGGAGATTGCCACCCCTGTAACATACACAATCAACAATGACCAGAATTGGGAGAGCATTCAAACGCCTTCGGATAATTATTACCAGAATTGGCAAAGCATTCCTAACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

247

Amino Acids

27.21

Weight (kDa)

5.86

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCAfunc PF19584 143 - 167 2.5e-07 MCAfunc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019889)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105791
rosa_multiflora Rmu_sc0001361.1_g000015 Rmu_sc0002776.1_g000036
rosa_roxburghii Rroxscaffold_1G00036870 Rroxscaffold_7G00185670
rosa_samantha Rh5AG012200 Rh5BG015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 231, 403
AccI GTMKAC 1 cut(s) 159
AccII CGCG 1 cut(s) 275
AccIII TCCGGA 1 cut(s) 338
AciI CCGC 4 cut(s) 113, 171, 229, 275
AclWI GGATC 2 cut(s) 22, 187
AcsI RAATTY 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 5 cut(s) 64, 81, 176, 209, 679
AgeI ACCGGT 1 cut(s) 456
AgsI TTSAA 3 cut(s) 496, 544, 692
AluBI AGCT 7 cut(s) 37, 326, 374, 418, 499, 598, 609
AluI AGCT 7 cut(s) 37, 326, 374, 418, 499, 598, 609
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 2 cut(s) 170, 336
AlwI GGATC 2 cut(s) 22, 187
AlwNI CAGNNNCTG 1 cut(s) 188
Ama87I CYCGRG 2 cut(s) 346, 375
Aor13HI TCCGGA 1 cut(s) 338
AoxI GGCC 1 cut(s) 200
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 296
AsiGI ACCGGT 1 cut(s) 456
AspLEI GCGC 3 cut(s) 146, 188, 275
AspS9I GGNCC 2 cut(s) 69, 410
AsuC2I CCSGG 2 cut(s) 76, 408
AsuHPI GGTGA 2 cut(s) 31, 52
AsuII TTCGAA 2 cut(s) 549, 585
AvaI CYCGRG 2 cut(s) 346, 375
AvaII GGWCC 2 cut(s) 69, 410
BaeGI GKGCMC 1 cut(s) 82
BanI GGYRCC 2 cut(s) 231, 403
BanII GRGCYC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 1 cut(s) 30
BccI CCATC 1 cut(s) 435
BceAI ACGGC 4 cut(s) 158, 169, 253, 338
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciVI GTATCC 1 cut(s) 346
BcnI CCSGG 2 cut(s) 76, 408
BcoDI GTCTC 2 cut(s) 170, 336
BfmI CTRYAG 2 cut(s) 16, 260
BfoI RGCGCY 1 cut(s) 189
BfuI GTATCC 1 cut(s) 346
BglII AGATCT 1 cut(s) 316
BisI GCNGC 3 cut(s) 19, 114, 276
BlsI GCNGC 3 cut(s) 20, 115, 277
Bme1390I CCNGG 2 cut(s) 76, 408
Bme18I GGWCC 2 cut(s) 69, 410
BmeT110I CYCGRG 2 cut(s) 346, 375
BmgT120I GGNCC 2 cut(s) 69, 410
BmiI GGNNCC 4 cut(s) 70, 233, 405, 411
BmrFI CCNGG 2 cut(s) 76, 408
BmsI GCATC 1 cut(s) 287
Bpu14I TTCGAA 2 cut(s) 549, 585
BpuEI CTTGAG 2 cut(s) 297, 312
BpuMI CCSGG 2 cut(s) 76, 408
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 5 cut(s) 63, 75, 126, 203, 235
BsaWI WCCGGW 3 cut(s) 338, 412, 456
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 81, 176, 209, 679
Bse118I RCCGGY 1 cut(s) 456
Bse3DI GCAATG 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 338
BseDI CCNNGG 5 cut(s) 63, 75, 126, 203, 235
BseGI GGATG 3 cut(s) 122, 278, 446
BseLI CCNNNNNNNGG 5 cut(s) 64, 81, 176, 209, 679
BseMI GCAATG 1 cut(s) 441
BseRI GAGGAG 3 cut(s) 138, 393, 608
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 1 cut(s) 30
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 202
BshNI GGYRCC 2 cut(s) 231, 403
BshTI ACCGGT 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 328
BsiHKCI CYCGRG 2 cut(s) 346, 375
BsiSI CCGG 7 cut(s) 75, 137, 339, 365, 407, 413, 457
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 5 cut(s) 64, 81, 176, 209, 679
BsmAI GTCTC 2 cut(s) 170, 336
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 423
BsmI GAATGC 3 cut(s) 250, 687, 729
BsnI GGCC 1 cut(s) 202
BsoBI CYCGRG 2 cut(s) 346, 375
Bsp119I TTCGAA 2 cut(s) 549, 585
Bsp1286I GDGCHC 2 cut(s) 82, 328
Bsp13I TCCGGA 1 cut(s) 338
Bsp143I GATC 4 cut(s) 9, 27, 179, 316
BspACI CCGC 4 cut(s) 113, 171, 229, 275
BspANI GGCC 1 cut(s) 202
BspEI TCCGGA 1 cut(s) 338
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 4 cut(s) 70, 233, 405, 411
BspMAI CTGCAG 2 cut(s) 20, 264
BspPI GGATC 2 cut(s) 22, 187
BspT104I TTCGAA 2 cut(s) 549, 585
BspT107I GGYRCC 2 cut(s) 231, 403
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 456
BssAI RCCGGY 1 cut(s) 456
BssECI CCNNGG 5 cut(s) 63, 75, 126, 203, 235
BssMI GATC 4 cut(s) 9, 27, 179, 316
BssNI GRCGYC 1 cut(s) 156
Bst4CI ACNGT 4 cut(s) 64, 164, 403, 537
BstACI GRCGYC 1 cut(s) 156
BstBI TTCGAA 2 cut(s) 549, 585
BstC8I GCNNGC 3 cut(s) 142, 264, 432
BstDSI CCRYGG 2 cut(s) 63, 235
BstF5I GGATG 3 cut(s) 122, 278, 446
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 3 cut(s) 146, 188, 275
BstKTI GATC 4 cut(s) 12, 30, 182, 319
BstMAI GTCTC 2 cut(s) 170, 336
BstMBI GATC 4 cut(s) 9, 27, 179, 316
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 3 cut(s) 137, 272, 284
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 2 cut(s) 74, 406
BstSFI CTRYAG 2 cut(s) 16, 260
BstSLI GKGCMC 1 cut(s) 82
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 1 cut(s) 202
BtgI CCRYGG 2 cut(s) 63, 235
BtsCI GGATG 3 cut(s) 122, 278, 446
Cac8I GCNNGC 3 cut(s) 142, 264, 432
CaiI CAGNNNCTG 1 cut(s) 188
CfoI GCGC 3 cut(s) 146, 188, 275
Cfr10I RCCGGY 1 cut(s) 456
Cfr13I GGNCC 2 cut(s) 69, 410
CseI GACGC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 454
CspAI ACCGGT 1 cut(s) 456
CviAII CATG 2 cut(s) 25, 141
CviQI GTAC 1 cut(s) 454
DpnI GATC 4 cut(s) 11, 29, 181, 318
DpnII GATC 4 cut(s) 9, 27, 179, 316
Ecl136II GAGCTC 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 328
Eco47I GGWCC 2 cut(s) 69, 410
Eco53kI GAGCTC 1 cut(s) 326
Eco88I CYCGRG 2 cut(s) 346, 375
EcoICRI GAGCTC 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 328
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 2 cut(s) 28, 144
FaiI YATR 5 cut(s) 26, 142, 387, 394, 655
FaqI GGGAC 1 cut(s) 423
FatI CATG 2 cut(s) 24, 140
FauI CCCGC 1 cut(s) 178
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 3 cut(s) 19, 114, 276
FokI GGATG 3 cut(s) 109, 265, 453
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 3 cut(s) 19, 114, 276
GlaI GCGC 3 cut(s) 145, 187, 274
GluI GCNGC 3 cut(s) 19, 114, 276
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 1 cut(s) 202
HapII CCGG 7 cut(s) 75, 137, 339, 365, 407, 413, 457
HgaI GACGC 1 cut(s) 145
HhaI GCGC 3 cut(s) 146, 188, 275
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 2 cut(s) 28, 144
Hin6I GCGC 3 cut(s) 144, 186, 273
HinP1I GCGC 3 cut(s) 144, 186, 273
HincII GTYRAC 2 cut(s) 160, 399
HindII GTYRAC 2 cut(s) 160, 399
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 3 cut(s) 85, 217, 344
HpaII CCGG 7 cut(s) 75, 137, 339, 365, 407, 413, 457
HphI GGTGA 2 cut(s) 31, 52
Hpy166II GTNNAC 5 cut(s) 6, 72, 160, 399, 454
Hpy188I TCNGA 6 cut(s) 32, 207, 283, 323, 474, 703
Hpy188III TCNNGA 6 cut(s) 314, 329, 339, 348, 463, 623
Hpy8I GTNNAC 5 cut(s) 6, 72, 160, 399, 454
Hpy99I CGWCG 2 cut(s) 161, 201
HpyAV CCTTC 4 cut(s) 52, 460, 581, 708
HpyCH4III ACNGT 4 cut(s) 64, 164, 403, 537
HpyCH4V TGCA 4 cut(s) 18, 248, 262, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 272, 284
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 2 cut(s) 28, 144
HspAI GCGC 3 cut(s) 144, 186, 273
Kpn2I TCCGGA 1 cut(s) 338
Kzo9I GATC 4 cut(s) 9, 27, 179, 316
LmnI GCTCC 5 cut(s) 151, 305, 323, 415, 595
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 1 cut(s) 287
MaeIII GTNAC 1 cut(s) 649
MalI GATC 4 cut(s) 11, 29, 181, 318
MboI GATC 4 cut(s) 9, 27, 179, 316
MboII GAAGA 1 cut(s) 266
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 2 cut(s) 82, 328
MluCI AATT 9 cut(s) 268, 296, 480, 504, 544, 627, 676, 706, 718
MlyI GAGTC 2 cut(s) 94, 338
MmeI TCCRAC 3 cut(s) 55, 157, 337
MroI TCCGGA 1 cut(s) 338
MseI TTAA 1 cut(s) 742
MspI CCGG 7 cut(s) 75, 137, 339, 365, 407, 413, 457
MspR9I CCNGG 2 cut(s) 76, 408
Mva1269I GAATGC 3 cut(s) 250, 687, 729
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 137, 272, 284
NciI CCSGG 2 cut(s) 76, 408
NdeII GATC 4 cut(s) 9, 27, 179, 316
NlaIII CATG 2 cut(s) 28, 144
NlaIV GGNNCC 4 cut(s) 70, 233, 405, 411
NmeAIII GCCGAG 1 cut(s) 107
NspI RCATGY 1 cut(s) 144
NspV TTCGAA 2 cut(s) 549, 585
PaeI GCATGC 1 cut(s) 144
PaeR7I CTCGAG 2 cut(s) 346, 375
PctI GAATGC 3 cut(s) 250, 687, 729
PfeI GAWTC 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 456
PkrI GCNGC 3 cut(s) 20, 115, 277
PleI GAGTC 2 cut(s) 93, 338
PpsI GAGTC 2 cut(s) 93, 338
Psp124BI GAGCTC 1 cut(s) 328
PspN4I GGNNCC 4 cut(s) 70, 233, 405, 411
PspPI GGNCC 2 cut(s) 69, 410
PspXI VCTCGAGB 1 cut(s) 375
PsrI GAACNNNNNNTAC 2 cut(s) 437, 469
PstI CTGCAG 2 cut(s) 20, 264
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SacI GAGCTC 1 cut(s) 328
SalI GTCGAC 1 cut(s) 158
SaqAI TTAA 1 cut(s) 742
SatI GCNGC 3 cut(s) 19, 114, 276
Sau3AI GATC 4 cut(s) 9, 27, 179, 316
Sau96I GGNCC 2 cut(s) 69, 410
SchI GAGTC 2 cut(s) 94, 338
ScrFI CCNGG 2 cut(s) 76, 408
SduI GDGCHC 2 cut(s) 82, 328
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 2 cut(s) 16, 260
Sfr274I CTCGAG 2 cut(s) 346, 375
SfuI TTCGAA 2 cut(s) 549, 585
SinI GGWCC 2 cut(s) 69, 410
SlaI CTCGAG 2 cut(s) 346, 375
SmlI CTYRAG 4 cut(s) 312, 327, 346, 375
SmoI CTYRAG 4 cut(s) 312, 327, 346, 375
SphI GCATGC 1 cut(s) 144
Sse9I AATT 9 cut(s) 268, 296, 480, 504, 544, 627, 676, 706, 718
SsiI CCGC 4 cut(s) 113, 171, 229, 275
SstI GAGCTC 1 cut(s) 328
StyD4I CCNGG 2 cut(s) 74, 406
TaaI ACNGT 4 cut(s) 64, 164, 403, 537
TaqI TCGA 7 cut(s) 159, 347, 376, 508, 549, 585, 622
TasI AATT 9 cut(s) 268, 296, 480, 504, 544, 627, 676, 706, 718
TauI GCSGC 2 cut(s) 116, 278
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 1 cut(s) 742
Tru9I TTAA 1 cut(s) 742
TseI GCWGC 1 cut(s) 18
TspDTI ATGAA 3 cut(s) 96, 111, 492
TspGWI ACGGA 2 cut(s) 97, 229
VpaK11BI GGWCC 2 cut(s) 69, 410
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 144
XhoI CTCGAG 2 cut(s) 346, 375
XmiI GTMKAC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.