Rmu_sc0003093.1_g000016

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003093.1
OG0019753
Physical Location & Seq
Reverse (-)
74659 .. 76524
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 390 bp
atgccgaccggtactattggttctagacctggtccattccttgctggttctggtagatttttagtcacagttcaaggaaaaggtggacatgctgcagctccccatttgactgccgacccgattcttgcagcctggtctgtcatccttgccctccaacagcttgtttctcgagaaaccgaccctctagagcccagggatgagtgggtctgcggtgatgatggcagtgatgggttacctgggtcatcgtttccaccatttaatgggttgtttgggttatctgggcctggagttagtgtttcagagatagaaggacacgacaatagctataggtttattgctccagcaaaaggccaattgttggaaattggtctcggtctcttagatagctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

129

Amino Acids

13.31

Weight (kDa)

4.63

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
M20_dimer PF07687 16 - 106 1.1e-06 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019753)

Species Orthologous Gene IDs
malus_domestica MD14G1033200.v1.1
rosa_multiflora Rmu_sc0003093.1_g000016
rosa_roxburghii Rroxscaffold_1G00013400 Rroxscaffold_2G00105580 Rroxscaffold_3G00232690
rosa_samantha Rh3CG220800 Rh3DG221700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 260, 358
AciI CCGC 1 cut(s) 210
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 3 cut(s) 260, 347, 358
AgeI ACCGGT 1 cut(s) 8
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 5 cut(s) 28, 131, 191, 235, 283
AluBI AGCT 4 cut(s) 98, 160, 324, 387
AluI AGCT 4 cut(s) 98, 160, 324, 387
Alw26I GTCTC 2 cut(s) 374, 380
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 2 cut(s) 281, 349
ApeKI GCWGC 3 cut(s) 92, 95, 128
AsiGI ACCGGT 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 32, 281
AsuHPI GGTGA 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 79, 107, 140
BccI CCATC 2 cut(s) 212, 221
BciT130I CCWGG 5 cut(s) 30, 133, 193, 237, 285
BcoDI GTCTC 2 cut(s) 374, 380
BfaI CTAG 2 cut(s) 24, 185
BfmI CTRYAG 2 cut(s) 93, 325
BisI GCNGC 3 cut(s) 93, 96, 129
BlsI GCNGC 3 cut(s) 94, 97, 130
Bme1390I CCNGG 5 cut(s) 30, 133, 193, 237, 285
Bme18I GGWCC 1 cut(s) 32
BmeT110I CYCGRG 1 cut(s) 168
BmgT120I GGNCC 2 cut(s) 32, 281
BmrFI CCNGG 5 cut(s) 30, 133, 193, 237, 285
BpmI CTGGAG 2 cut(s) 306, 324
BsaI GGTCTC 2 cut(s) 374, 380
BsaJI CCNNGG 3 cut(s) 191, 192, 236
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 347, 358
Bse118I RCCGGY 1 cut(s) 8
BseBI CCWGG 5 cut(s) 30, 133, 193, 237, 285
BseDI CCNNGG 3 cut(s) 191, 192, 236
BseGI GGATG 2 cut(s) 141, 202
BseLI CCNNNNNNNGG 3 cut(s) 260, 347, 358
BseXI GCAGC 3 cut(s) 79, 107, 140
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 283, 351
BshTI ACCGGT 1 cut(s) 8
BsiEI CGRYCG 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 1 cut(s) 9
BslI CCNNNNNNNGG 3 cut(s) 260, 347, 358
BsmAI GTCTC 2 cut(s) 374, 380
BsnI GGCC 2 cut(s) 283, 351
Bso31I GGTCTC 2 cut(s) 374, 380
BsoBI CYCGRG 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 192
BspACI CCGC 1 cut(s) 210
BspANI GGCC 2 cut(s) 283, 351
BspMAI CTGCAG 1 cut(s) 97
BspTNI GGTCTC 2 cut(s) 374, 380
BsrFI RCCGGY 1 cut(s) 8
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 3 cut(s) 191, 192, 236
Bst2UI CCWGG 5 cut(s) 30, 133, 193, 237, 285
Bst4CI ACNGT 1 cut(s) 70
BstDEI CTNAG 1 cut(s) 379
BstEII GGTNACC 1 cut(s) 231
BstF5I GGATG 2 cut(s) 141, 202
BstMAI GTCTC 2 cut(s) 374, 380
BstMCI CGRYCG 1 cut(s) 9
BstNI CCWGG 5 cut(s) 30, 133, 193, 237, 285
BstNSI RCATGY 1 cut(s) 92
BstPI GGTNACC 1 cut(s) 231
BstSCI CCNGG 5 cut(s) 28, 131, 191, 235, 283
BstSFI CTRYAG 2 cut(s) 93, 325
BstV1I GCAGC 3 cut(s) 79, 107, 140
BsuRI GGCC 2 cut(s) 283, 351
BtsCI GGATG 2 cut(s) 141, 202
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 2 cut(s) 32, 281
CsiI ACCWGGT 1 cut(s) 28
Csp6I GTAC 1 cut(s) 12
CspAI ACCGGT 1 cut(s) 8
CviAII CATG 1 cut(s) 89
CviJI RGCY 8 cut(s) 98, 131, 160, 190, 283, 324, 351, 387
CviKI_1 RGCY 8 cut(s) 98, 131, 160, 190, 283, 324, 351, 387
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 2 cut(s) 374, 380
Eco47I GGWCC 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 168
Eco91I GGTNACC 1 cut(s) 231
EcoO65I GGTNACC 1 cut(s) 231
EcoRII CCWGG 5 cut(s) 28, 131, 191, 235, 283
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 1 cut(s) 92
FaiI YATR 2 cut(s) 90, 327
FatI CATG 1 cut(s) 88
Fnu4HI GCNGC 3 cut(s) 93, 96, 129
FokI GGATG 2 cut(s) 128, 209
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 3 cut(s) 93, 96, 129
FspBI CTAG 2 cut(s) 24, 185
GluI GCNGC 3 cut(s) 93, 96, 129
GsuI CTGGAG 2 cut(s) 306, 324
HaeIII GGCC 2 cut(s) 283, 351
HapII CCGG 1 cut(s) 9
Hin1II CATG 1 cut(s) 92
HinfI GANTC 1 cut(s) 121
HpaII CCGG 1 cut(s) 9
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 1 cut(s) 86
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 4 cut(s) 24, 168, 170, 185
Hpy8I GTNNAC 1 cut(s) 86
HpyAV CCTTC 1 cut(s) 302
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 95, 128
HpyF3I CTNAG 1 cut(s) 379
Hsp92II CATG 1 cut(s) 92
LmnI GCTCC 2 cut(s) 103, 343
Lsp1109I GCAGC 3 cut(s) 79, 107, 140
MabI ACCWGGT 1 cut(s) 28
MaeI CTAG 2 cut(s) 24, 185
MaeIII GTNAC 2 cut(s) 64, 231
MfeI CAATTG 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 353, 363
MmeI TCCRAC 2 cut(s) 178, 339
MnlI CCTC 2 cut(s) 161, 192
MseI TTAA 1 cut(s) 258
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 5 cut(s) 30, 133, 193, 237, 285
MunI CAATTG 1 cut(s) 353
MvaI CCWGG 5 cut(s) 30, 133, 193, 237, 285
NlaIII CATG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 92
PaeR7I CTCGAG 1 cut(s) 168
PasI CCCWGGG 1 cut(s) 192
PfeI GAWTC 1 cut(s) 121
PflFI GACNNNGTC 1 cut(s) 30
PflMI CCANNNNNTGG 2 cut(s) 260, 358
PinAI ACCGGT 1 cut(s) 8
PkrI GCNGC 3 cut(s) 94, 97, 130
Psp6I CCWGG 5 cut(s) 28, 131, 191, 235, 283
PspEI GGTNACC 1 cut(s) 231
PspGI CCWGG 5 cut(s) 28, 131, 191, 235, 283
PspPI GGNCC 2 cut(s) 32, 281
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstI CTGCAG 1 cut(s) 97
PsyI GACNNNGTC 1 cut(s) 30
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 3 cut(s) 93, 96, 129
Sau96I GGNCC 2 cut(s) 32, 281
ScrFI CCNGG 5 cut(s) 30, 133, 193, 237, 285
SduI GDGCHC 1 cut(s) 192
SetI ASST 8 cut(s) 31, 85, 100, 162, 238, 326, 332, 389
SexAI ACCWGGT 1 cut(s) 28
SfcI CTRYAG 2 cut(s) 93, 325
Sfr274I CTCGAG 1 cut(s) 168
SinI GGWCC 1 cut(s) 32
SlaI CTCGAG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
Sse9I AATT 2 cut(s) 353, 363
SsiI CCGC 1 cut(s) 210
SspMI CTAG 2 cut(s) 24, 185
StyD4I CCNGG 5 cut(s) 28, 131, 191, 235, 283
TaaI ACNGT 1 cut(s) 70
TaqI TCGA 1 cut(s) 169
TaqII GACCGA 1 cut(s) 362
TasI AATT 2 cut(s) 353, 363
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 3 cut(s) 92, 95, 128
Tsp45I GTSAC 1 cut(s) 64
TspRI CASTG 1 cut(s) 229
Tth111I GACNNNGTC 1 cut(s) 30
Van91I CCANNNNNTGG 2 cut(s) 260, 358
VpaK11BI GGWCC 1 cut(s) 32
XbaI TCTAGA 2 cut(s) 23, 184
XceI RCATGY 1 cut(s) 92
XhoI CTCGAG 1 cut(s) 168
XspI CTAG 2 cut(s) 24, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.