Rmu_sc0003337.1_g000036

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003337.1
OG0022941
Physical Location & Seq
Reverse (-)
149694 .. 151232
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003337.1_g000036.1.cds

Sequence Viewer

Length: 696 bp
atggaacccatactcgatttgatagcttgttcaatagagaacaacaacaaccaatccttctggcaattcagcgaccagcttcgtgtccagacatccaatctcgccaacctttccgtcaacgacttgatctggagcagctcatacggtagcaagagacctgatgcgaggaggaacttcgacatcaaggttggcagtgaggtgaattcttcgattaatttgaccccaaaagggtttgggttcaatgaaggattggactacaagcagaaagggtcagacccgaatgagggctggaacagcttcaagcccaaggcttctgaattcagcgacaggtttaacaaagcttcagacttcaattctttcaacccaaaagcttcacacttcaattctttcaacccaaaagtttcagactttaatgcgtttaaccagaaagtcccgaacttcaatgggtttaatcagaagctgatcgagtatccgactatggttcatgccttctatatttttccgaagttgctggaatccaaccagctcatttcgcaagtcaaggatttcgttgcttcagttgaggacaataaatttagacaccggaatctcgacaccagtccggtcgccagatttcggtcaccggttgccggattcccatcacaggagtcagctgccggcaactggtcaccgaagtccggtaaggtggaggaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

231

Amino Acids

26.13

Weight (kDa)

7.8

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022941)

Species Orthologous Gene IDs
rosa_laevigata RLG00000015611
rosa_multiflora Rmu_sc0003337.1_g000036
rosa_samantha Rh5CG376000 Rh6CG105200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 202, 317, 572
AcuI CTGAAG 2 cut(s) 327, 540
AfiI CCNNNNNNNGG 8 cut(s) 228, 283, 284, 615, 629, 643, 663, 677
AgeI ACCGGT 1 cut(s) 622
AgsI TTSAA 8 cut(s) 33, 241, 301, 352, 361, 382, 391, 442
AluBI AGCT 9 cut(s) 26, 79, 138, 297, 341, 371, 460, 526, 653
AluI AGCT 9 cut(s) 26, 79, 138, 297, 341, 371, 460, 526, 653
Alw26I GTCTC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 460
ApeKI GCWGC 2 cut(s) 135, 653
ApoI RAATTY 3 cut(s) 202, 317, 572
AseI ATTAAT 1 cut(s) 213
AsiGI ACCGGT 1 cut(s) 622
Asp700I GAANNNNTTC 1 cut(s) 296
AsuHPI GGTGA 3 cut(s) 211, 612, 660
BbvI GCAGC 2 cut(s) 147, 640
BccI CCATC 1 cut(s) 646
BciVI GTATCC 1 cut(s) 480
BcoDI GTCTC 1 cut(s) 148
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 2 cut(s) 136, 654
BlsI GCNGC 2 cut(s) 137, 655
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 1 cut(s) 151
BoxI GACNNNNGTC 1 cut(s) 597
BpmI CTGGAG 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 637
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 306
BsaWI WCCGGW 4 cut(s) 582, 601, 622, 677
Bsc4I CCNNNNNNNGG 8 cut(s) 228, 283, 284, 615, 629, 643, 663, 677
Bse118I RCCGGY 2 cut(s) 622, 656
Bse1I ACTGG 2 cut(s) 597, 668
Bse8I GATNNNNATC 1 cut(s) 637
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 92
BseJI GATNNNNATC 1 cut(s) 637
BseLI CCNNNNNNNGG 8 cut(s) 228, 283, 284, 615, 629, 643, 663, 677
BseNI ACTGG 2 cut(s) 597, 668
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 2 cut(s) 147, 640
Bsh1285I CGRYCG 1 cut(s) 606
BshTI ACCGGT 1 cut(s) 622
BsiEI CGRYCG 1 cut(s) 606
BsiSI CCGG 6 cut(s) 583, 602, 623, 630, 657, 678
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 8 cut(s) 228, 283, 284, 615, 629, 643, 663, 677
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 416
Bso31I GGTCTC 1 cut(s) 148
Bsp143I GATC 2 cut(s) 126, 462
BspLI GGNNCC 1 cut(s) 6
BspTNI GGTCTC 1 cut(s) 148
BsrFI RCCGGY 2 cut(s) 622, 656
BsrI ACTGG 2 cut(s) 597, 668
BssAI RCCGGY 2 cut(s) 622, 656
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 2 cut(s) 126, 462
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 658
BstEII GGTNACC 2 cut(s) 618, 666
BstF5I GGATG 1 cut(s) 92
BstKTI GATC 2 cut(s) 129, 465
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 2 cut(s) 126, 462
BstMCI CGRYCG 1 cut(s) 606
BstMWI GCNNNNNNNGC 2 cut(s) 294, 532
BstPAI GACNNNNGTC 1 cut(s) 597
BstPI GGTNACC 2 cut(s) 618, 666
BstV1I GCAGC 2 cut(s) 147, 640
BsuI GTATCC 1 cut(s) 480
BtsCI GGATG 1 cut(s) 92
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 658
CaiI CAGNNNCTG 1 cut(s) 460
Cfr10I RCCGGY 2 cut(s) 622, 656
CspAI ACCGGT 1 cut(s) 622
CviAII CATG 1 cut(s) 485
DpnI GATC 2 cut(s) 128, 464
DpnII GATC 2 cut(s) 126, 462
Eco130I CCWWGG 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 327, 540
Eco91I GGTNACC 2 cut(s) 618, 666
EcoO65I GGTNACC 2 cut(s) 618, 666
EcoRI GAATTC 2 cut(s) 202, 317
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 488
FaiI YATR 5 cut(s) 11, 142, 479, 486, 495
FaqI GGGAC 1 cut(s) 416
FatI CATG 1 cut(s) 484
Fnu4HI GCNGC 2 cut(s) 136, 654
FokI GGATG 1 cut(s) 79
Fsp4HI GCNGC 2 cut(s) 136, 654
GluI GCNGC 2 cut(s) 136, 654
GsuI CTGGAG 1 cut(s) 151
HapII CCGG 6 cut(s) 583, 602, 623, 630, 657, 678
Hin1II CATG 1 cut(s) 488
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 2 cut(s) 339, 369
HinfI GANTC 4 cut(s) 515, 586, 633, 647
HpaII CCGG 6 cut(s) 583, 602, 623, 630, 657, 678
HphI GGTGA 3 cut(s) 211, 612, 660
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 7 cut(s) 274, 316, 346, 406, 456, 474, 504
Hpy188III TCNNGA 4 cut(s) 88, 130, 433, 590
Hpy8I GTNNAC 1 cut(s) 118
HpyAV CCTTC 3 cut(s) 67, 239, 499
HpyCH4III ACNGT 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 2 cut(s) 294, 532
Hsp92II CATG 1 cut(s) 488
KroI GCCGGC 1 cut(s) 656
KroNI GCCGGC 1 cut(s) 658
Kzo9I GATC 2 cut(s) 126, 462
LmnI GCTCC 1 cut(s) 132
Lsp1109I GCAGC 2 cut(s) 147, 640
LweI GCATC 1 cut(s) 151
MaeIII GTNAC 2 cut(s) 618, 666
MalI GATC 2 cut(s) 128, 464
MboI GATC 2 cut(s) 126, 462
MboII GAAGA 1 cut(s) 198
MluCI AATT 7 cut(s) 65, 202, 214, 317, 352, 382, 572
MlyI GAGTC 1 cut(s) 656
MmeI TCCRAC 2 cut(s) 497, 543
MnlI CCTC 6 cut(s) 159, 162, 190, 277, 556, 682
MroNI GCCGGC 1 cut(s) 656
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 5 cut(s) 213, 333, 411, 420, 450
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 6 cut(s) 583, 602, 623, 630, 657, 678
MwoI GCNNNNNNNGC 2 cut(s) 294, 532
NaeI GCCGGC 1 cut(s) 658
NdeII GATC 2 cut(s) 126, 462
NgoMIV GCCGGC 1 cut(s) 656
NlaIII CATG 1 cut(s) 488
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 618, 666
PdiI GCCGGC 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 3 cut(s) 515, 586, 633
PinAI ACCGGT 1 cut(s) 622
PkrI GCNGC 2 cut(s) 137, 655
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PshAI GACNNNNGTC 1 cut(s) 597
PshBI ATTAAT 1 cut(s) 213
PspEI GGTNACC 2 cut(s) 618, 666
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 653
SaqAI TTAA 5 cut(s) 213, 333, 411, 420, 450
SatI GCNGC 2 cut(s) 136, 654
Sau3AI GATC 2 cut(s) 126, 462
SchI GAGTC 1 cut(s) 656
SfaNI GCATC 1 cut(s) 151
Sse9I AATT 7 cut(s) 65, 202, 214, 317, 352, 382, 572
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 146
TaqI TCGA 5 cut(s) 15, 177, 209, 465, 591
TaqII GACCGA 1 cut(s) 606
TasI AATT 7 cut(s) 65, 202, 214, 317, 352, 382, 572
TfiI GAWTC 3 cut(s) 515, 586, 633
Tru1I TTAA 5 cut(s) 213, 333, 411, 420, 450
Tru9I TTAA 5 cut(s) 213, 333, 411, 420, 450
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 2 cut(s) 618, 666
TseI GCWGC 2 cut(s) 135, 653
Tsp45I GTSAC 2 cut(s) 618, 666
TspDTI ATGAA 2 cut(s) 258, 473
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 1 cut(s) 199
VspI ATTAAT 1 cut(s) 213
XapI RAATTY 3 cut(s) 202, 317, 572
XmnI GAANNNNTTC 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.