Rh6CG105200

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0022941
Physical Location & Seq
Reverse (-)
11861584 .. 11869727
8144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG105200.1

Sequence Viewer

Length: 609 bp
ATGATCTCCACCGGCGTGACTTCATTAGTTTTCAGATTTTCTGGGTTTCTAGATCAAGATTTGATAGCTTGTTCAATAGAGAACAACAACAACCAATCCTTCTGGCAATTCAGCGACCAGCTTCGTGTCCAGACATCCAATCTCGCCAACCTTTCCGTCAACGACTCGATCTGGAGCAGCTCATACTGTAGCAAGAGGCCTGATGCGAGGAGGAACTTCGACATCAAGGTCGGCGGTGAGGTGAATTCTTCGATTAATTTGACCCCAAAAGGGTCTGGGTTCAATGAAGGATTGGACTACAAGCAGAAAGGGTCCGACCCGAATGAGGGCTGGAACAGCTTCAAGCCCAAGGCTTCTGAATTCAGCGACAGGTTTAACAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGCTTCAGACTTCAATTCTTTCAACCCAAAAGTTTCAGACTTTAATGCGTTTAACCAGAAAGTTCCGGACTTCAATGGATTTAATCAGAAGCTGATAGAGTATCCGGCTATGGTTCACTCCTTCTATATTTTTCCGAAGTTGCCGGAATCCAACCAGCTCATTTCGCAAGTTAAGGATTTCGTTGCTTCAGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

202

Amino Acids

22.7

Weight (kDa)

5.85

Isoelectric Point (pI)

31.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022941)

Species Orthologous Gene IDs
rosa_laevigata RLG00000015611
rosa_multiflora Rmu_sc0003337.1_g000036
rosa_samantha Rh5CG376000 Rh6CG105200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccIII TCCGGA 1 cut(s) 475
AciI CCGC 1 cut(s) 234
AcsI RAATTY 2 cut(s) 244, 359
AcuI CTGAAG 3 cut(s) 369, 399, 582
AfiI CCNNNNNNNGG 3 cut(s) 270, 325, 326
AgsI TTSAA 8 cut(s) 75, 283, 343, 394, 403, 424, 433, 484
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 8 cut(s) 68, 121, 180, 339, 383, 413, 502, 568
AluI AGCT 8 cut(s) 68, 121, 180, 339, 383, 413, 502, 568
AlwNI CAGNNNCTG 1 cut(s) 502
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 1 cut(s) 177
ApoI RAATTY 2 cut(s) 244, 359
AseI ATTAAT 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 338
AspS9I GGNCC 1 cut(s) 312
AsuHPI GGTGA 2 cut(s) 248, 253
AvaII GGWCC 1 cut(s) 312
BbvI GCAGC 1 cut(s) 189
BciVI GTATCC 1 cut(s) 522
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 187
BfuI GTATCC 1 cut(s) 522
BisI GCNGC 1 cut(s) 178
BlsI GCNGC 1 cut(s) 179
Bme18I GGWCC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 1 cut(s) 313
BmsI GCATC 1 cut(s) 193
BpmI CTGGAG 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 348
BsaWI WCCGGW 1 cut(s) 475
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 325, 326
Bse118I RCCGGY 1 cut(s) 11
BseAI TCCGGA 1 cut(s) 475
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 1 cut(s) 134
BseLI CCNNNNNNNGG 3 cut(s) 270, 325, 326
BseRI GAGGAG 1 cut(s) 223
BseXI GCAGC 1 cut(s) 189
BshFI GGCC 1 cut(s) 199
BsiSI CCGG 4 cut(s) 12, 476, 515, 554
BslI CCNNNNNNNGG 3 cut(s) 270, 325, 326
BsnI GGCC 1 cut(s) 199
Bsp13I TCCGGA 1 cut(s) 475
Bsp143I GATC 3 cut(s) 3, 52, 168
BspACI CCGC 1 cut(s) 234
BspANI GGCC 1 cut(s) 199
BspEI TCCGGA 1 cut(s) 475
BspLI GGNNCC 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 3 cut(s) 3, 52, 168
BssT1I CCWWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 188
BstF5I GGATG 1 cut(s) 134
BstKTI GATC 3 cut(s) 6, 55, 171
BstMBI GATC 3 cut(s) 3, 52, 168
BstMWI GCNNNNNNNGC 2 cut(s) 336, 574
BstSFI CTRYAG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 189
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 134
CaiI CAGNNNCTG 1 cut(s) 502
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 312
DpnI GATC 3 cut(s) 5, 54, 170
DpnII GATC 3 cut(s) 3, 52, 168
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 312
Eco57I CTGAAG 3 cut(s) 369, 399, 582
EcoRI GAATTC 2 cut(s) 244, 359
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
FaiI YATR 3 cut(s) 184, 521, 537
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 1 cut(s) 50
GluI GCNGC 1 cut(s) 178
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 1 cut(s) 199
HapII CCGG 4 cut(s) 12, 476, 515, 554
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 2 cut(s) 381, 411
HinfI GANTC 2 cut(s) 164, 557
HpaII CCGG 4 cut(s) 12, 476, 515, 554
HphI GGTGA 2 cut(s) 248, 253
Hpy166II GTNNAC 2 cut(s) 160, 526
Hpy188I TCNGA 8 cut(s) 35, 316, 358, 388, 418, 448, 498, 546
Hpy188III TCNNGA 5 cut(s) 50, 56, 130, 172, 476
Hpy8I GTNNAC 2 cut(s) 160, 526
HpyAV CCTTC 3 cut(s) 109, 281, 541
HpyCH4III ACNGT 1 cut(s) 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 336, 574
Kpn2I TCCGGA 1 cut(s) 475
Kzo9I GATC 3 cut(s) 3, 52, 168
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 16
MalI GATC 3 cut(s) 5, 54, 170
MboI GATC 3 cut(s) 3, 52, 168
MboII GAAGA 1 cut(s) 240
MluCI AATT 6 cut(s) 107, 244, 256, 359, 394, 424
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 2 cut(s) 339, 585
MnlI CCTC 5 cut(s) 189, 201, 204, 232, 319
MroI TCCGGA 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 6 cut(s) 255, 375, 453, 462, 492, 582
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 4 cut(s) 12, 476, 515, 554
MwoI GCNNNNNNNGC 2 cut(s) 336, 574
NdeII GATC 3 cut(s) 3, 52, 168
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 16
OliI CACNNNNGTG 1 cut(s) 14
PceI AGGCCT 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 557
PkrI GCNGC 1 cut(s) 179
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PshBI ATTAAT 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 502
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 6 cut(s) 255, 375, 453, 462, 492, 582
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 3 cut(s) 3, 52, 168
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 1 cut(s) 158
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 187
SgrAI CRCCGGYG 1 cut(s) 11
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 6 cut(s) 107, 244, 256, 359, 394, 424
SseBI AGGCCT 1 cut(s) 199
SsiI CCGC 1 cut(s) 234
SspMI CTAG 1 cut(s) 50
StuI AGGCCT 1 cut(s) 199
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 188
TaqI TCGA 3 cut(s) 167, 219, 251
TasI AATT 6 cut(s) 107, 244, 256, 359, 394, 424
TfiI GAWTC 1 cut(s) 557
Tru1I TTAA 6 cut(s) 255, 375, 453, 462, 492, 582
Tru9I TTAA 6 cut(s) 255, 375, 453, 462, 492, 582
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 1 cut(s) 177
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 2 cut(s) 12, 300
TspGWI ACGGA 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 312
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 2 cut(s) 244, 359
XbaI TCTAGA 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.