Rh5CG376000

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0022941
Physical Location & Seq
Reverse (-)
48662035 .. 48663809
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG376000.1

Sequence Viewer

Length: 486 bp
ATGGAGAACAACAACAACCAATCCTTCTGGCAATTCAGCGACCAGCTTCGTGTCCAGACATCCAATCTCGCCAACCTTTCCGTCAACGACTCGATCTGGAGCAGCTCATACGGCAGCAAGAGGCCTGATGCGAGGAGGAACTTCGACATCAAGGTCGGCGGTGAGGTGAATTCTTTGATTAATTTGACCCCAAAAGGGTCTGGGTTCAATGAAGGATTGGACTACAAGCAGAAAGGGTCCGACCCGAATGAGGGCTGGAACAGCTTCAAGCCCAAGGCTTCTGAATTCAGGGACAGGTTTAACAAAGCTTCTGACTTCAATTCTTTCAAAATGGAGGTTCCGGTGAGCTCGCGACGGCGCTGCAGTCAACTTTGGCCAACTTGTGGGATTCTCACGATTCGTGAGTGTAGCTTCACCAATTTCCTTGTTGACTATGATGGAGTAGGCGAAGTCTTGAAGGTACCACTCAATTGCCTCTCGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

161

Amino Acids

18.22

Weight (kDa)

8.36

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022941)

Species Orthologous Gene IDs
rosa_laevigata RLG00000015611
rosa_multiflora Rmu_sc0003337.1_g000036
rosa_samantha Rh5CG376000 Rh6CG105200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
Acc65I GGTACC 1 cut(s) 460
AccB1I GGYRCC 1 cut(s) 460
AccB7I CCANNNNNTGG 1 cut(s) 383
AccII CGCG 1 cut(s) 352
AciI CCGC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 374
AcsI RAATTY 2 cut(s) 169, 284
AfaI GTAC 1 cut(s) 462
AfiI CCNNNNNNNGG 4 cut(s) 195, 250, 251, 383
AgsI TTSAA 5 cut(s) 208, 268, 319, 328, 457
AluBI AGCT 6 cut(s) 46, 105, 264, 308, 348, 411
AluI AGCT 6 cut(s) 46, 105, 264, 308, 348, 411
Alw21I GWGCWC 1 cut(s) 350
AoxI GGCC 2 cut(s) 122, 374
ApeKI GCWGC 3 cut(s) 102, 114, 360
ApoI RAATTY 2 cut(s) 169, 284
AseI ATTAAT 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 263
Asp718I GGTACC 1 cut(s) 460
AspLEI GCGC 1 cut(s) 360
AspS9I GGNCC 1 cut(s) 237
AsuHPI GGTGA 4 cut(s) 173, 178, 355, 406
AvaII GGWCC 1 cut(s) 237
BalI TGGCCA 1 cut(s) 376
BanI GGYRCC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 3 cut(s) 114, 126, 347
BccI CCATC 1 cut(s) 431
BceAI ACGGC 2 cut(s) 127, 371
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BfaI CTAG 1 cut(s) 484
BfmI CTRYAG 1 cut(s) 361
BfoI RGCGCY 1 cut(s) 361
BisI GCNGC 3 cut(s) 103, 115, 361
BlsI GCNGC 3 cut(s) 104, 116, 362
Bme18I GGWCC 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 237
BmiI GGNNCC 3 cut(s) 238, 339, 462
BmsI GCATC 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 118
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 250, 251, 383
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 4 cut(s) 195, 250, 251, 383
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 3 cut(s) 114, 126, 347
Bsh1236I CGCG 1 cut(s) 352
BshFI GGCC 2 cut(s) 124, 376
BshNI GGYRCC 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 1 cut(s) 341
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 4 cut(s) 195, 250, 251, 383
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 124, 376
Bsp1286I GDGCHC 1 cut(s) 350
Bsp143I GATC 1 cut(s) 93
Bsp68I TCGCGA 1 cut(s) 352
BspACI CCGC 1 cut(s) 159
BspANI GGCC 2 cut(s) 124, 376
BspFNI CGCG 1 cut(s) 352
BspLI GGNNCC 3 cut(s) 238, 339, 462
BspMAI CTGCAG 1 cut(s) 365
BspT107I GGYRCC 1 cut(s) 460
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 350
BstF5I GGATG 1 cut(s) 59
BstFNI CGCG 1 cut(s) 352
BstH2I RGCGCY 1 cut(s) 361
BstHHI GCGC 1 cut(s) 360
BstKTI GATC 1 cut(s) 96
BstMBI GATC 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 111, 261
BstSFI CTRYAG 1 cut(s) 361
BstUI CGCG 1 cut(s) 352
BstV1I GCAGC 3 cut(s) 114, 126, 347
BsuRI GGCC 2 cut(s) 124, 376
BtsCI GGATG 1 cut(s) 59
BtuMI TCGCGA 1 cut(s) 352
Cac8I GCNNGC 1 cut(s) 350
CfoI GCGC 1 cut(s) 360
Cfr13I GGNCC 1 cut(s) 237
Csp6I GTAC 1 cut(s) 461
CviQI GTAC 1 cut(s) 461
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 374
Ecl136II GAGCTC 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 348
EcoRI GAATTC 2 cut(s) 169, 284
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 1 cut(s) 273
FaiI YATR 2 cut(s) 109, 435
FaqI GGGAC 1 cut(s) 305
Fnu4HI GCNGC 3 cut(s) 103, 115, 361
FokI GGATG 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 3 cut(s) 103, 115, 361
FspBI CTAG 1 cut(s) 484
GlaI GCGC 1 cut(s) 359
GluI GCNGC 3 cut(s) 103, 115, 361
GsuI CTGGAG 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 361
HaeIII GGCC 2 cut(s) 124, 376
HapII CCGG 1 cut(s) 341
HhaI GCGC 1 cut(s) 360
Hin6I GCGC 1 cut(s) 358
HinP1I GCGC 1 cut(s) 358
HincII GTYRAC 3 cut(s) 85, 368, 430
HindII GTYRAC 3 cut(s) 85, 368, 430
HindIII AAGCTT 1 cut(s) 306
HinfI GANTC 3 cut(s) 89, 388, 397
HpaII CCGG 1 cut(s) 341
HphI GGTGA 4 cut(s) 173, 178, 355, 406
Hpy166II GTNNAC 3 cut(s) 85, 368, 430
Hpy188I TCNGA 3 cut(s) 241, 283, 313
Hpy188III TCNNGA 6 cut(s) 55, 97, 351, 394, 401, 454
Hpy8I GTNNAC 3 cut(s) 85, 368, 430
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 3 cut(s) 34, 206, 451
HpyCH4V TGCA 1 cut(s) 363
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 261
HspAI GCGC 1 cut(s) 358
KpnI GGTACC 1 cut(s) 464
Kzo9I GATC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 3 cut(s) 114, 126, 347
LweI GCATC 1 cut(s) 118
MaeI CTAG 1 cut(s) 484
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MfeI CAATTG 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 350
MlsI TGGCCA 1 cut(s) 376
MluCI AATT 7 cut(s) 32, 169, 181, 284, 319, 418, 469
MluNI TGGCCA 1 cut(s) 376
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 7 cut(s) 114, 126, 129, 157, 244, 328, 485
Mox20I TGGCCA 1 cut(s) 376
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 376
MseI TTAA 2 cut(s) 180, 300
Msp20I TGGCCA 1 cut(s) 376
MspI CCGG 1 cut(s) 341
MunI CAATTG 1 cut(s) 469
MvnI CGCG 1 cut(s) 352
MwoI GCNNNNNNNGC 2 cut(s) 111, 261
NdeII GATC 1 cut(s) 93
NlaIV GGNNCC 3 cut(s) 238, 339, 462
NruI TCGCGA 1 cut(s) 352
PceI AGGCCT 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 388, 397
PflMI CCANNNNNTGG 1 cut(s) 383
PkrI GCNGC 3 cut(s) 104, 116, 362
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PshBI ATTAAT 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 350
PspN4I GGNNCC 3 cut(s) 238, 339, 462
PspPI GGNCC 1 cut(s) 237
PstI CTGCAG 1 cut(s) 365
RruI TCGCGA 1 cut(s) 352
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
SacI GAGCTC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 180, 300
SatI GCNGC 3 cut(s) 103, 115, 361
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 1 cut(s) 237
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 1 cut(s) 118
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 1 cut(s) 237
Sse9I AATT 7 cut(s) 32, 169, 181, 284, 319, 418, 469
SseBI AGGCCT 1 cut(s) 124
SsiI CCGC 1 cut(s) 159
SspMI CTAG 1 cut(s) 484
SstI GAGCTC 1 cut(s) 350
StuI AGGCCT 1 cut(s) 124
StyI CCWWGG 1 cut(s) 273
TaqI TCGA 2 cut(s) 92, 144
TaqII GACCGA 1 cut(s) 469
TasI AATT 7 cut(s) 32, 169, 181, 284, 319, 418, 469
TfiI GAWTC 2 cut(s) 388, 397
Tru1I TTAA 2 cut(s) 180, 300
Tru9I TTAA 2 cut(s) 180, 300
TseI GCWGC 3 cut(s) 102, 114, 360
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 1 cut(s) 70
Van91I CCANNNNNTGG 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 237
VspI ATTAAT 1 cut(s) 180
XapI RAATTY 2 cut(s) 169, 284
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.