Rmu_sc0003731.1_g000003

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003731.1
OG0021656
Physical Location & Seq
Forward (+)
13495 .. 14247
753 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
atggagaaacaagaaaatggaggtgaagatggcattgttgccaagaagaaggtatgtttctccattccttcctcttgcttctcttttggccaagtcaatcgagtagttcatcgccgtatggatattgctgtcaggataaaagaactaaatgaggagttcattttgattgctagtcaaaaacaaaattatagctttcaaaacctgaaaagagacttggaacaacttgaaccaaaactttcttcccctgttgatatagtatttggtcgggaatacgaaaagaatattttactacataagttgttgactgcaaattgtcatgaacttaggggtccccttatgatccccatgatagggatgcgaggaattggaaagacaactctttccaaactagtctataatgatgaaaaggtcaaggctcattttgacaagagagtatgggtctctgtctcagaccctcttggtgagatcaaagttgccaaagccatcattgaaggtcttggtagtactagtaccccaaattcaactgagttgcaaactcttctacaatgcatacatcaattgtttgaggaaaagaaattcctccttgttttagatcatgtgtggaatccaaatcttactgggtggaaacaattaagcagatctttatttaacggagatataggcagtataatattggtgacatcacaaaatgaggaggttgctatcatgatgggagcagtcgatcccatgatccatttgaaggagtttaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

250

Amino Acids

28.33

Weight (kDa)

8.27

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 94 - 244 2.5e-26 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021656)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002589.1_g000044 Rmu_sc0003731.1_g000003 Rmu_sc0003731.1_g000012
rosa_rugosa Rorug02G0232800 Rorug02G0298300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 334, 716, 724
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 2 cut(s) 517, 575
AfaI GTAC 2 cut(s) 505, 511
AfiI CCNNNNNNNGG 3 cut(s) 350, 351, 739
AgsI TTSAA 5 cut(s) 197, 227, 491, 522, 739
AhlI ACTAGT 2 cut(s) 388, 506
AjuI GAANNNNNNNTTGG 4 cut(s) 197, 223, 229, 255
AluBI AGCT 1 cut(s) 192
AluI AGCT 1 cut(s) 192
Alw26I GTCTC 3 cut(s) 204, 445, 451
AlwI GGATC 3 cut(s) 334, 716, 724
AoxI GGCC 1 cut(s) 88
ApoI RAATTY 2 cut(s) 517, 575
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 3 cut(s) 35, 473, 688
AvaII GGWCC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 90
BccI CCATC 3 cut(s) 23, 491, 703
BceAI ACGGC 1 cut(s) 99
BcoDI GTCTC 3 cut(s) 204, 445, 451
BcuI ACTAGT 2 cut(s) 388, 506
BfaI CTAG 3 cut(s) 171, 389, 507
BglII AGATCT 1 cut(s) 638
BmcAI AGTACT 1 cut(s) 505
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 2 cut(s) 330, 331
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 1 cut(s) 345
BmuI ACTGGG 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 445
BsaXI ACNNNNNCTCC 1 cut(s) 734
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 351, 739
Bse1I ACTGG 1 cut(s) 622
BseGI GGATG 1 cut(s) 360
BseLI CCNNNNNNNGG 3 cut(s) 350, 351, 739
BseMII CTCAG 2 cut(s) 462, 516
BseNI ACTGG 1 cut(s) 622
BseRI GAGGAG 2 cut(s) 167, 707
BshFI GGCC 1 cut(s) 90
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 3 cut(s) 350, 351, 739
BsmAI GTCTC 3 cut(s) 204, 445, 451
BsmFI GGGAC 1 cut(s) 315
BsnI GGCC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 445
Bsp143I GATC 6 cut(s) 339, 465, 592, 638, 721, 729
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 2 cut(s) 461, 517
BspHI TCATGA 2 cut(s) 316, 705
BspLI GGNNCC 2 cut(s) 330, 331
BspPI GGATC 3 cut(s) 334, 716, 724
BspTNI GGTCTC 1 cut(s) 445
BsrI ACTGG 1 cut(s) 622
BssMI GATC 6 cut(s) 339, 465, 592, 638, 721, 729
Bst6I CTCTTC 1 cut(s) 543
BstDEI CTNAG 3 cut(s) 323, 448, 525
BstF5I GGATG 1 cut(s) 360
BstKTI GATC 6 cut(s) 342, 468, 595, 641, 724, 732
BstMAI GTCTC 3 cut(s) 204, 445, 451
BstMBI GATC 6 cut(s) 339, 465, 592, 638, 721, 729
BstX2I RGATCY 1 cut(s) 638
BstYI RGATCY 1 cut(s) 638
BsuRI GGCC 1 cut(s) 90
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 1 cut(s) 360
CciI TCATGA 2 cut(s) 316, 705
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 504, 510
CviAII CATG 5 cut(s) 317, 346, 596, 706, 727
CviJI RGCY 4 cut(s) 90, 192, 416, 482
CviKI_1 RGCY 4 cut(s) 90, 192, 416, 482
CviQI GTAC 2 cut(s) 504, 510
DdeI CTNAG 3 cut(s) 323, 448, 525
DpnI GATC 6 cut(s) 341, 467, 594, 640, 723, 731
DpnII GATC 6 cut(s) 339, 465, 592, 638, 721, 729
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Eco31I GGTCTC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 329
EcoT22I ATGCAT 1 cut(s) 551
FaeI CATG 5 cut(s) 320, 349, 599, 709, 730
FalI AAGNNNNNCTT 2 cut(s) 625, 657
FaqI GGGAC 1 cut(s) 315
FatI CATG 5 cut(s) 316, 345, 595, 705, 726
FokI GGATG 1 cut(s) 367
FspBI CTAG 3 cut(s) 171, 389, 507
HaeIII GGCC 1 cut(s) 90
Hin1II CATG 5 cut(s) 320, 349, 599, 709, 730
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HinfI GANTC 1 cut(s) 604
HphI GGTGA 3 cut(s) 35, 473, 688
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 1 cut(s) 451
Hpy188III TCNNGA 4 cut(s) 133, 266, 317, 706
Hpy8I GTNNAC 1 cut(s) 303
HpyAV CCTTC 4 cut(s) 43, 78, 485, 733
HpyCH4V TGCA 3 cut(s) 308, 532, 549
HpyF3I CTNAG 3 cut(s) 323, 448, 525
Hsp92II CATG 5 cut(s) 320, 349, 599, 709, 730
KflI GGGWCCC 1 cut(s) 329
Kzo9I GATC 6 cut(s) 339, 465, 592, 638, 721, 729
LmnI GCTCC 1 cut(s) 713
LpnPI CCDG 4 cut(s) 118, 215, 258, 603
LweI GCATC 1 cut(s) 345
MaeI CTAG 3 cut(s) 171, 389, 507
MaeIII GTNAC 1 cut(s) 676
MalI GATC 6 cut(s) 341, 467, 594, 640, 723, 731
MboI GATC 6 cut(s) 339, 465, 592, 638, 721, 729
MboII GAAGA 4 cut(s) 38, 58, 231, 530
MfeI CAATTG 1 cut(s) 557
MflI RGATCY 1 cut(s) 638
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 7 cut(s) 184, 310, 363, 517, 557, 575, 629
MluNI TGGCCA 1 cut(s) 90
MnlI CCTC 9 cut(s) 14, 82, 145, 353, 465, 559, 590, 685, 688
Mox20I TGGCCA 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 551
MscI TGGCCA 1 cut(s) 90
MseI TTAA 3 cut(s) 632, 648, 747
Msp20I TGGCCA 1 cut(s) 90
MunI CAATTG 1 cut(s) 557
NdeII GATC 6 cut(s) 339, 465, 592, 638, 721, 729
NlaIII CATG 5 cut(s) 320, 349, 599, 709, 730
NlaIV GGNNCC 2 cut(s) 330, 331
NmuCI GTSAC 1 cut(s) 676
NsiI ATGCAT 1 cut(s) 551
PagI TCATGA 2 cut(s) 316, 705
PfeI GAWTC 1 cut(s) 604
PpuMI RGGWCCY 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 329
PspN4I GGNNCC 2 cut(s) 330, 331
PspPI GGNCC 1 cut(s) 329
PspPPI RGGWCCY 1 cut(s) 329
PsuI RGATCY 1 cut(s) 638
RsaI GTAC 2 cut(s) 505, 511
RsaNI GTAC 2 cut(s) 504, 510
SaqAI TTAA 3 cut(s) 632, 648, 747
Sau3AI GATC 6 cut(s) 339, 465, 592, 638, 721, 729
Sau96I GGNCC 1 cut(s) 329
ScaI AGTACT 1 cut(s) 505
SetI ASST 7 cut(s) 25, 54, 194, 204, 411, 496, 699
SfaNI GCATC 1 cut(s) 345
SinI GGWCC 1 cut(s) 329
SpeI ACTAGT 2 cut(s) 388, 506
Sse9I AATT 7 cut(s) 184, 310, 363, 517, 557, 575, 629
SspI AATATT 2 cut(s) 283, 672
SspMI CTAG 3 cut(s) 171, 389, 507
TaqI TCGA 2 cut(s) 100, 720
TasI AATT 7 cut(s) 184, 310, 363, 517, 557, 575, 629
TatI WGTACW 1 cut(s) 503
TfiI GAWTC 1 cut(s) 604
Tru1I TTAA 3 cut(s) 632, 648, 747
Tru9I TTAA 3 cut(s) 632, 648, 747
TseFI GTSAC 1 cut(s) 676
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 4 cut(s) 98, 148, 333, 417
TspGWI ACGGA 1 cut(s) 666
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 2 cut(s) 517, 575
XspI CTAG 3 cut(s) 171, 389, 507
ZrmI AGTACT 1 cut(s) 505
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.