Rmu_sc0003808.1_g000011

Fanconi anaemia protein FancD2 nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003808.1
OG0006206
Physical Location & Seq
Reverse (-)
51052 .. 60428
9377 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3831 bp
atgcttctcgagaagcttccggagtatttccatggggattcggatcagaatccatcgctttctcttgaagatgatgttgctaggcttataatcaaccacttccggtggctcgatttcgttgttgattctaatgcattggctgagaaattgatgcaagtgctctcgatttgccctttgcatttgaagaaggagatcattggatcactgccggagattattggggatcaggataacaagactgtggttcagtctctggagaaaatgcttgaagaggactcggctgtagttgtggctgtgcttgattccttctcgaaccttaatttggataatgagttgcaagaacaggttatcaccatagcattgtcctgcatcagaacaattgatgcggagcatatgccctacctgcttagatttttattgctttcagcaacaccattgaatgtaaggcgaataatctctcagatacgtgcgaagctgaagtttgttggtgtatcaagccatcatgcatcacaacagagtaaactcaaaggaaagtctcgggtggacaacacagaggcttctattcttgatgccttgagatcaagtcttagatttaagaatatactgtgtcaggagatcttgaaagacttgaactgtcttgagaaacctcaggatcacaaagtcattgacatatggctacttatgctgatatacatgaatggcgaatctttgcaaaggagtgttgaaaagctatttaagaaaaaagttattgaagattgtattcaggaagctatgtttgatcaatgcattcatgggcacaaggagctcgtacaggattattttctatctttcatttctttgtctgagtacctgttggcttgcaaagagcagaaggccaggcactttagcattcatatgtatgtttgtctctttgaggagtttgatgatacttattcaagacaggaagttcttggttcactagttacccatgtgggctctagtgtgggttttgaagtcagttcttctttagagaccatgaggttgctggtttccaaatatgcacagcaattaattcctctttcttctcatattagtggtatcttggattatttagagggattcagtcttgaaaatctgcacaaggtagaatgctatcattctttattattcttggctcaaaacttaaaatcatcattcactgattttaccaccatgaaggtctatgagattttcagccatctggcccttttggctcggtcaagtgtagattcatatggttcttcctttgcaaatgaacttttcatgatagtacgtaagcaggttagtcatccagatctgaactacaagaagatgggccttgttgggactctgaagatagtttcctgtctaggatatgcagctaatgatacatatccatccccatctcagaaatcaaactgtgatgaggctctggaactcttgaaaacagctttggactcttgcaaacagttgcccttacctctgatcatgttttatgacgaactgactgaaatgatggataataacacacttgatccaatagttatggaatggattggcaaacatgtaggagagtttgaatctatatttctttctgatcttgacggtgggaaattagcagccaacgaatattgtggtttagaaggtgaactgtggatgaacttggacggtgatatatctcctatttgtctaaacattttgccattggcttcatctacattgcagtctgcttcttctttacaagttcttcctgctaacttccttctattatctgcaattgaaagcctgtcagtgctcatatcaatgaccaatactgacatgcaactacttgtggattcccagtatttctttggatctgagtggccgtctctcacagggaagcagaaacagattgtatgtggctctctattttatgcttctaattggatacgggaactactcaatgccttctgtacacaaatcaccggaagatttgaatttacaagtcaggctactaaggaggacataattgccaagcttttgaagcgtgtgaggaatcttatatttttggaaagcatattaaacaattgtattgagaggtactctctacctcttcctgaacttcatccacatgcggatatcaatcagtcttctctcttaaaccaacctcaccatatgaggcacactgaaaagaaactcgagcataagaagacacaagaggacatctctccaagcggagcgaggagaaataataagacctcaaaagcatcaacaacatcagatactatgggaaaactccggcagcccacattatttgatgtcttggagaaagcgggagttataacaggccaagaggtgccaaatgaggattcttccggtttatctacaaagggaaggtcatctgagtcatctcagaaaaatccctgtgactttaatgaggctgccattatagaaatgtctgctgttgccaaggcccttgaagctcagagatctaaattcagacctctcctggttcaatgttattcagttttagcaatttcaaagagtgaagcgtcctgttgctctgatgctgcagctgagttacccctacatctgtaccttctgcgtgatctgcacaacaagctggagtacttcaatcctggaaaacagttgtggggtaaatgcttgagtggtccagttgggttcacaaggatgacattagatgaattcgtgagcagaattgaaccactctttccaagtcttaagaaacatttggatagtgcagtttggttacttaaagaaggtgatgaaacttgtgaagaacattggaataatgaatctactgctgctggaaatccagacattcccaatcttgtactttcaaactacacagcttctacttcagtattcaaagaagtacttcattgtttcagtgcgatattaaaccttcctggtcttcaaacagacaagccagctctctcacacctgctagaagcatttcagcccgataagatttcagatggtttttttgcagggatacagcctaacctttctcctgggactgcagaatatttatattttggagcttattcattctttgaaggtgtcttggacatagcatgttctttctcctttatgctggcttcagagtcactttttactctagaatcggttgtaacctcagtccagaaatttgtccgtaagttggagggaaatggtaaaagcatgcagtacggatttatccaggaggctgttcctactttgcgcagaaaacttggtacttctgcggagaagattttaaggcatagatgggatgatgacaatcttgataatggttggaagagcaaaggagaaacagtacagaagatattgcgtatttacctggataatagcggctctactcctgatctgctggatgagctctcctgctctattttaccccaggcttcttttggaaaaggaaaggaagaagatgatcatcatggttttccttctctatcttctggaacatttggtgtatggtacaagatattggttttagagcttcaaaaagccacaagaacaatacagaccctatgttctgaagcaaagggcttgagacaaacaagtataaccagaaaaatccctgctacaaagagatctatggaacgctttttgttctgtgtgaaagctcttctccacactgcaccaagcggccttactttctggatgggaaatttgaagcataaaaatttgacgggtgaggtcgtgagttcccaagtatatgcggatcatgataacgagaacagtgctgatgaggagccagatgaggctgttgatgaatatccaccggagagtgttgcagttgaagaggacgcagagacattgaccgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FancD2

Protein Analysis

1276

Amino Acids

143.11

Weight (kDa)

5.32

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FancD2 PF14631 1 - 389 1.3e-74 Fanconi anaemia protein FancD2 nuclease
FancD2 PF14631 395 - 573 1.7e-14 Fanconi anaemia protein FancD2 nuclease
FancD2 PF14631 621 - 750 2.8e-08 Fanconi anaemia protein FancD2 nuclease
FancD2 PF14631 1168 - 1250 4e-13 Fanconi anaemia protein FancD2 nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 89, 2318
AarI CACCTGC 1 cut(s) 2949
Acc16I TGCGCA 1 cut(s) 3221
Acc36I ACCTGC 3 cut(s) 411, 1288, 2949
AccB1I GGYRCC 1 cut(s) 2332
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 8 cut(s) 386, 2111, 2211, 2309, 3242, 3348, 3648, 3722
AclWI GGATC 7 cut(s) 51, 208, 231, 660, 1526, 1858, 3732
AcoI YGGCCR 1 cut(s) 1859
AcsI RAATTY 6 cut(s) 1973, 2471, 2681, 3146, 3670, 3685
AcuI CTGAAG 5 cut(s) 497, 1370, 2841, 3084, 3558
AfiI CCNNNNNNNGG 2 cut(s) 322, 3160
AflII CTTAAG 1 cut(s) 2717
AflIII ACRYGT 1 cut(s) 1561
AhlI ACTAGT 1 cut(s) 958
AjnI CCWGG 8 cut(s) 875, 2484, 2614, 2905, 3010, 3198, 3336, 3396
AjuI GAANNNNNNNTTGG 6 cut(s) 305, 337, 1433, 1465, 2329, 2361
Alw21I GWGCWC 4 cut(s) 162, 807, 1794, 3378
Alw26I GTCTC 7 cut(s) 255, 540, 911, 1004, 1869, 3547, 3809
AlwI GGATC 7 cut(s) 51, 208, 231, 660, 1526, 1858, 3732
AlwNI CAGNNNCTG 1 cut(s) 253
Ama87I CYCGRG 3 cut(s) 8, 537, 2174
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 7 cut(s) 873, 1221, 1333, 1859, 2323, 2448, 3649
ApeKI GCWGC 7 cut(s) 1376, 1616, 2278, 2417, 2546, 2549, 2801
ApoI RAATTY 6 cut(s) 1973, 2471, 2681, 3146, 3670, 3685
ArsI GACNNNNNNTTYG 2 cut(s) 2357, 2389
AseI ATTAAT 1 cut(s) 1049
Asp700I GAANNNNTTC 2 cut(s) 2874, 2952
AspLEI GCGC 1 cut(s) 3222
AspS9I GGNCC 4 cut(s) 1222, 1333, 2449, 2648
AsuHPI GGTGA 7 cut(s) 343, 1655, 1679, 1951, 2138, 2771, 3707
AvaI CYCGRG 3 cut(s) 8, 537, 2174
AvaII GGWCC 1 cut(s) 2648
AxyI CCTNAGG 1 cut(s) 648
BaeGI GKGCMC 1 cut(s) 798
BaeI ACNNNNGTAYC 2 cut(s) 2555, 2588
BanI GGYRCC 1 cut(s) 2332
BanII GRGCYC 3 cut(s) 807, 977, 3378
BbsI GAAGAC 3 cut(s) 2118, 2192, 2903
Bbv12I GWGCWC 4 cut(s) 162, 807, 1794, 3378
BbvI GCAGC 7 cut(s) 1388, 1628, 2290, 2404, 2533, 2561, 2788
BceAI ACGGC 1 cut(s) 1846
BcgI CGANNNNNNTGC 2 cut(s) 3725, 3759
BciT130I CCWGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
BciVI GTATCC 2 cut(s) 1917, 2985
BclI TGATCA 3 cut(s) 778, 1482, 3430
BcoDI GTCTC 7 cut(s) 255, 540, 911, 1004, 1869, 3547, 3809
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 6 cut(s) 81, 959, 978, 1367, 2945, 3119
BfmI CTRYAG 3 cut(s) 282, 2547, 3018
BfrI CTTAAG 1 cut(s) 2717
BfuAI ACCTGC 3 cut(s) 411, 1288, 2949
BfuI GTATCC 2 cut(s) 1917, 2985
BglI GCCNNNNNGGC 1 cut(s) 1229
BglII AGATCT 4 cut(s) 615, 1312, 2465, 3593
BisI GCNGC 9 cut(s) 1377, 1617, 2279, 2418, 2547, 2550, 2802, 3349, 3649
BlsI GCNGC 9 cut(s) 1378, 1618, 2280, 2419, 2548, 2551, 2803, 3350, 3650
BmcAI AGTACT 2 cut(s) 2606, 2874
Bme1390I CCNGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
Bme18I GGWCC 1 cut(s) 2648
BmeT110I CYCGRG 3 cut(s) 8, 537, 2174
BmgT120I GGNCC 4 cut(s) 1222, 1333, 2449, 2648
BmiI GGNNCC 2 cut(s) 2334, 3756
BmrFI CCNGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
BmrI ACTGGG 1 cut(s) 1831
BmsI GCATC 7 cut(s) 141, 373, 378, 515, 559, 2252, 2533
BmuI ACTGGG 1 cut(s) 1831
BpiI GAAGAC 3 cut(s) 2118, 2192, 2903
BplI GAGNNNNNCTC 6 cut(s) 2063, 2095, 2065, 2097, 2186, 2218
BpmI CTGGAG 2 cut(s) 275, 2621
BpuEI CTTGAG 4 cut(s) 595, 659, 2662, 3571
BsaAI YACGTR 2 cut(s) 467, 1292
BsaBI GATNNNNATC 7 cut(s) 42, 48, 1389, 2118, 3276, 3432, 3777
BsaI GGTCTC 1 cut(s) 1004
BsaJI CCNNGG 4 cut(s) 31, 2445, 3011, 3396
BsaWI WCCGGW 5 cut(s) 19, 102, 1961, 2351, 3784
BsaXI ACNNNNNCTCC 2 cut(s) 3297, 3327
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 3160
Bse1I ACTGG 2 cut(s) 1837, 2651
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 1 cut(s) 1715
Bse8I GATNNNNATC 7 cut(s) 42, 48, 1389, 2118, 3276, 3432, 3777
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
BseDI CCNNGG 4 cut(s) 31, 2445, 3011, 3396
BseGI GGATG 8 cut(s) 1306, 1394, 1659, 2101, 2673, 3274, 3376, 3669
BseJI GATNNNNATC 7 cut(s) 42, 48, 1389, 2118, 3276, 3432, 3777
BseLI CCNNNNNNNGG 2 cut(s) 322, 3160
BseMI GCAATG 1 cut(s) 1715
BseNI ACTGG 2 cut(s) 1837, 2651
BseRI GAGGAG 3 cut(s) 929, 2233, 3767
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 7 cut(s) 1388, 1628, 2290, 2404, 2533, 2561, 2788
BsgI GTGCAG 4 cut(s) 1100, 2573, 2757, 3624
BshFI GGCC 7 cut(s) 875, 1223, 1335, 1861, 2325, 2450, 3651
BshNI GGYRCC 1 cut(s) 2332
BsiHKAI GWGCWC 4 cut(s) 162, 807, 1794, 3378
BsiHKCI CYCGRG 3 cut(s) 8, 537, 2174
BsiSI CCGG 7 cut(s) 20, 103, 209, 1962, 2275, 2352, 3785
BslFI GGGAC 2 cut(s) 1357, 3028
BslI CCNNNNNNNGG 2 cut(s) 322, 3160
BsmAI GTCTC 7 cut(s) 255, 540, 911, 1004, 1869, 3547, 3809
BsmBI CGTCTC 1 cut(s) 1869
BsmFI GGGAC 2 cut(s) 1357, 3028
BsmI GAATGC 3 cut(s) 786, 888, 1133
BsnI GGCC 7 cut(s) 875, 1223, 1335, 1861, 2325, 2450, 3651
Bso31I GGTCTC 1 cut(s) 1004
BsoBI CYCGRG 3 cut(s) 8, 537, 2174
Bsp1286I GDGCHC 6 cut(s) 162, 798, 807, 977, 1794, 3378
Bsp13I TCCGGA 1 cut(s) 19
Bsp1407I TGTACA 1 cut(s) 1949
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 8 cut(s) 386, 2111, 2211, 2309, 3242, 3348, 3648, 3722
BspANI GGCC 7 cut(s) 875, 1223, 1335, 1861, 2325, 2450, 3651
BspEI TCCGGA 1 cut(s) 19
BspHI TCATGA 2 cut(s) 1281, 3727
BspLI GGNNCC 2 cut(s) 2334, 3756
BspMAI CTGCAG 2 cut(s) 2551, 3022
BspMI ACCTGC 3 cut(s) 411, 1288, 2949
BspPI GGATC 7 cut(s) 51, 208, 231, 660, 1526, 1858, 3732
BspQI GCTCTTC 2 cut(s) 3290, 3633
BspT107I GGYRCC 1 cut(s) 2332
BspTI CTTAAG 1 cut(s) 2717
BspTNI GGTCTC 1 cut(s) 1004
BsrDI GCAATG 1 cut(s) 1715
BsrGI TGTACA 1 cut(s) 1949
BsrI ACTGG 2 cut(s) 1837, 2651
BssECI CCNNGG 4 cut(s) 31, 2445, 3011, 3396
BssT1I CCWWGG 2 cut(s) 31, 2445
Bst2UI CCWGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
Bst6I CTCTTC 5 cut(s) 264, 2094, 3290, 3633, 3798
BstAFI CTTAAG 1 cut(s) 2717
BstAUI TGTACA 1 cut(s) 1949
BstBAI YACGTR 2 cut(s) 467, 1292
BstC8I GCNNGC 4 cut(s) 859, 2928, 3096, 3182
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 8 cut(s) 1306, 1394, 1659, 2101, 2673, 3274, 3376, 3669
BstHHI GCGC 1 cut(s) 3222
BstMAI GTCTC 7 cut(s) 255, 540, 911, 1004, 1869, 3547, 3809
BstMWI GCNNNNNNNGC 9 cut(s) 403, 682, 802, 1229, 2020, 2456, 2587, 2596, 3373
BstNI CCWGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
BstNSI RCATGY 5 cut(s) 1565, 1819, 2111, 3078, 3184
BstSCI CCNGG 8 cut(s) 875, 2484, 2614, 2905, 3010, 3198, 3336, 3396
BstSFI CTRYAG 3 cut(s) 282, 2547, 3018
BstSLI GKGCMC 1 cut(s) 798
BstSNI TACGTA 1 cut(s) 1292
BstV1I GCAGC 7 cut(s) 1388, 1628, 2290, 2404, 2533, 2561, 2788
BstV2I GAAGAC 3 cut(s) 2118, 2192, 2903
BstX2I RGATCY 5 cut(s) 615, 1312, 1850, 2465, 3593
BstYI RGATCY 5 cut(s) 615, 1312, 1850, 2465, 3593
Bsu36I CCTNAGG 1 cut(s) 648
BsuI GTATCC 2 cut(s) 1917, 2985
BsuRI GGCC 7 cut(s) 875, 1223, 1335, 1861, 2325, 2450, 3651
BtgI CCRYGG 1 cut(s) 31
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 8 cut(s) 1306, 1394, 1659, 2101, 2673, 3274, 3376, 3669
BtsI GCAGTG 2 cut(s) 203, 3636
BtsIMutI CAGTG 7 cut(s) 203, 1176, 1794, 2160, 2893, 3636, 3748
BveI ACCTGC 3 cut(s) 411, 1288, 2949
Cac8I GCNNGC 4 cut(s) 859, 2928, 3096, 3182
CaiI CAGNNNCTG 1 cut(s) 253
CciI TCATGA 2 cut(s) 1281, 3727
CfoI GCGC 1 cut(s) 3222
Cfr13I GGNCC 4 cut(s) 1222, 1333, 2449, 2648
CseI GACGC 2 cut(s) 2517, 3818
CspCI CAANNNNNGTGG 2 cut(s) 2272, 2307
EaeI YGGCCR 1 cut(s) 1859
Eam1104I CTCTTC 5 cut(s) 264, 2094, 3290, 3633, 3798
EarI CTCTTC 5 cut(s) 264, 2094, 3290, 3633, 3798
Ecl136II GAGCTC 2 cut(s) 805, 3376
Eco105I TACGTA 1 cut(s) 1292
Eco130I CCWWGG 2 cut(s) 31, 2445
Eco24I GRGCYC 3 cut(s) 807, 977, 3378
Eco31I GGTCTC 1 cut(s) 1004
Eco32I GATATC 1 cut(s) 2116
Eco47I GGWCC 1 cut(s) 2648
Eco53kI GAGCTC 2 cut(s) 805, 3376
Eco57I CTGAAG 5 cut(s) 497, 1370, 2841, 3084, 3558
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 3 cut(s) 8, 537, 2174
EcoICRI GAGCTC 2 cut(s) 805, 3376
EcoO109I RGGNCCY 1 cut(s) 2449
EcoRI GAATTC 1 cut(s) 2681
EcoRII CCWGG 8 cut(s) 875, 2484, 2614, 2905, 3010, 3198, 3336, 3396
EcoRV GATATC 1 cut(s) 2116
EcoT14I CCWWGG 2 cut(s) 31, 2445
EcoT22I ATGCAT 3 cut(s) 136, 508, 788
EcoT38I GRGCYC 3 cut(s) 807, 977, 3378
ErhI CCWWGG 2 cut(s) 31, 2445
Esp3I CGTCTC 1 cut(s) 1869
FalI AAGNNNNNCTT 2 cut(s) 2859, 2891
FaqI GGGAC 2 cut(s) 1357, 3028
FauI CCCGC 1 cut(s) 2302
FauNDI CATATG 5 cut(s) 393, 671, 894, 1252, 2151
FbaI TGATCA 3 cut(s) 778, 1482, 3430
Fnu4HI GCNGC 9 cut(s) 1377, 1617, 2279, 2418, 2547, 2550, 2802, 3349, 3649
FokI GGATG 8 cut(s) 1293, 1381, 1666, 2088, 2680, 3281, 3383, 3676
FriOI GRGCYC 3 cut(s) 807, 977, 3378
Fsp4HI GCNGC 9 cut(s) 1377, 1617, 2279, 2418, 2547, 2550, 2802, 3349, 3649
FspBI CTAG 6 cut(s) 81, 959, 978, 1367, 2945, 3119
FspI TGCGCA 1 cut(s) 3221
GlaI GCGC 1 cut(s) 3221
GluI GCNGC 9 cut(s) 1377, 1617, 2279, 2418, 2547, 2550, 2802, 3349, 3649
GsuI CTGGAG 2 cut(s) 275, 2621
HaeIII GGCC 7 cut(s) 875, 1223, 1335, 1861, 2325, 2450, 3651
HapII CCGG 7 cut(s) 20, 103, 209, 1962, 2275, 2352, 3785
HgaI GACGC 2 cut(s) 2517, 3818
HhaI GCGC 1 cut(s) 3222
Hin6I GCGC 1 cut(s) 3220
HinP1I GCGC 1 cut(s) 3220
HindIII AAGCTT 2 cut(s) 14, 2012
HpaII CCGG 7 cut(s) 20, 103, 209, 1962, 2275, 2352, 3785
HphI GGTGA 7 cut(s) 343, 1655, 1679, 1951, 2138, 2771, 3707
Hpy166II GTNNAC 6 cut(s) 521, 544, 956, 1646, 1952, 2661
Hpy8I GTNNAC 6 cut(s) 521, 544, 956, 1646, 1952, 2661
HpyCH4IV ACGT 2 cut(s) 466, 1291
HpyF10VI GCNNNNNNNGC 9 cut(s) 403, 682, 802, 1229, 2020, 2456, 2587, 2596, 3373
HpySE526I ACGT 2 cut(s) 466, 1291
HspAI GCGC 1 cut(s) 3220
Kpn2I TCCGGA 1 cut(s) 19
Ksp22I TGATCA 3 cut(s) 778, 1482, 3430
LguI GCTCTTC 2 cut(s) 3290, 3633
LmnI GCTCC 5 cut(s) 388, 802, 2213, 3038, 3754
Lsp1109I GCAGC 7 cut(s) 1388, 1628, 2290, 2404, 2533, 2561, 2788
LweI GCATC 7 cut(s) 141, 373, 378, 515, 559, 2252, 2533
MaeI CTAG 6 cut(s) 81, 959, 978, 1367, 2945, 3119
MaeII ACGT 2 cut(s) 466, 1291
MaeIII GTNAC 6 cut(s) 961, 2402, 2556, 2745, 3105, 3130
MfeI CAATTG 3 cut(s) 378, 1773, 2062
MflI RGATCY 5 cut(s) 615, 1312, 1850, 2465, 3593
MhlI GDGCHC 6 cut(s) 162, 798, 807, 977, 1794, 3378
MlyI GAGTC 5 cut(s) 269, 1339, 1448, 2390, 3113
MmeI TCCRAC 2 cut(s) 3141, 3272
Mph1103I ATGCAT 3 cut(s) 136, 508, 788
MroI TCCGGA 1 cut(s) 19
MroXI GAANNNNTTC 2 cut(s) 2874, 2952
MslI CAYNNNNRTG 6 cut(s) 893, 897, 1071, 1799, 2106, 2666
MspA1I CMGCKG 1 cut(s) 2552
MspCI CTTAAG 1 cut(s) 2717
MspI CCGG 7 cut(s) 20, 103, 209, 1962, 2275, 2352, 3785
MspR9I CCNGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
MunI CAATTG 3 cut(s) 378, 1773, 2062
Mva1269I GAATGC 3 cut(s) 786, 888, 1133
MvaI CCWGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
MwoI GCNNNNNNNGC 9 cut(s) 403, 682, 802, 1229, 2020, 2456, 2587, 2596, 3373
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 5 cut(s) 393, 671, 894, 1252, 2151
NlaIV GGNNCC 2 cut(s) 2334, 3756
NmeAIII GCCGAG 1 cut(s) 257
NmuCI GTSAC 2 cut(s) 2402, 3105
NsbI TGCGCA 1 cut(s) 3221
NsiI ATGCAT 3 cut(s) 136, 508, 788
NspI RCATGY 5 cut(s) 1565, 1819, 2111, 3078, 3184
PaeI GCATGC 1 cut(s) 3184
PaeR7I CTCGAG 2 cut(s) 8, 2174
PagI TCATGA 2 cut(s) 1281, 3727
PaqCI CACCTGC 1 cut(s) 2949
PciI ACATGT 1 cut(s) 1561
PciSI GCTCTTC 2 cut(s) 3290, 3633
PctI GAATGC 3 cut(s) 786, 888, 1133
PdmI GAANNNNTTC 2 cut(s) 2874, 2952
PfoI TCCNGGA 2 cut(s) 2614, 3198
PkrI GCNGC 9 cut(s) 1378, 1618, 2280, 2419, 2548, 2551, 2803, 3350, 3650
PleI GAGTC 5 cut(s) 269, 1339, 1448, 2389, 3112
PpsI GAGTC 5 cut(s) 269, 1339, 1448, 2389, 3112
Ppu21I YACGTR 2 cut(s) 467, 1292
PscI ACATGT 1 cut(s) 1561
PshBI ATTAAT 1 cut(s) 1049
PsiI TTATAA 2 cut(s) 89, 2318
Psp124BI GAGCTC 2 cut(s) 807, 3378
Psp6I CCWGG 8 cut(s) 875, 2484, 2614, 2905, 3010, 3198, 3336, 3396
PspGI CCWGG 8 cut(s) 875, 2484, 2614, 2905, 3010, 3198, 3336, 3396
PspN4I GGNNCC 2 cut(s) 2334, 3756
PspPI GGNCC 4 cut(s) 1222, 1333, 2449, 2648
PspXI VCTCGAGB 1 cut(s) 2174
PstI CTGCAG 2 cut(s) 2551, 3022
PstNI CAGNNNCTG 1 cut(s) 253
PsuI RGATCY 5 cut(s) 615, 1312, 1850, 2465, 3593
PvuII CAGCTG 1 cut(s) 2552
RseI CAYNNNNRTG 6 cut(s) 893, 897, 1071, 1799, 2106, 2666
SacI GAGCTC 2 cut(s) 807, 3378
SapI GCTCTTC 2 cut(s) 3290, 3633
SatI GCNGC 9 cut(s) 1377, 1617, 2279, 2418, 2547, 2550, 2802, 3349, 3649
Sau96I GGNCC 4 cut(s) 1222, 1333, 2449, 2648
ScaI AGTACT 2 cut(s) 2606, 2874
SchI GAGTC 5 cut(s) 269, 1339, 1448, 2390, 3113
ScrFI CCNGG 8 cut(s) 877, 2486, 2616, 2907, 3012, 3200, 3338, 3398
SduI GDGCHC 6 cut(s) 162, 798, 807, 977, 1794, 3378
SfaNI GCATC 7 cut(s) 141, 373, 378, 515, 559, 2252, 2533
SfcI CTRYAG 3 cut(s) 282, 2547, 3018
Sfr274I CTCGAG 2 cut(s) 8, 2174
SinI GGWCC 1 cut(s) 2648
SlaI CTCGAG 2 cut(s) 8, 2174
SmiMI CAYNNNNRTG 6 cut(s) 893, 897, 1071, 1799, 2106, 2666
SmlI CTYRAG 7 cut(s) 8, 574, 638, 2174, 2641, 2717, 3550
SmoI CTYRAG 7 cut(s) 8, 574, 638, 2174, 2641, 2717, 3550
SnaBI TACGTA 1 cut(s) 1292
SpeI ACTAGT 1 cut(s) 958
SphI GCATGC 1 cut(s) 3184
SsiI CCGC 8 cut(s) 386, 2111, 2211, 2309, 3242, 3348, 3648, 3722
SspI AATATT 2 cut(s) 1628, 3026
SspMI CTAG 6 cut(s) 81, 959, 978, 1367, 2945, 3119
SstI GAGCTC 2 cut(s) 807, 3378
StyD4I CCNGG 8 cut(s) 875, 2484, 2614, 2905, 3010, 3198, 3336, 3396
StyI CCWWGG 2 cut(s) 31, 2445
TaiI ACGT 2 cut(s) 469, 1294
TaqI TCGA 5 cut(s) 9, 111, 164, 311, 2175
TaqII GACCGA 1 cut(s) 1224
TatI WGTACW 5 cut(s) 1949, 2604, 2830, 2872, 3313
TauI GCSGC 2 cut(s) 3351, 3651
TscAI CASTG 7 cut(s) 210, 1183, 1794, 2167, 2893, 3643, 3748
TseFI GTSAC 2 cut(s) 2402, 3105
TseI GCWGC 7 cut(s) 1376, 1616, 2278, 2417, 2546, 2549, 2801
Tsp45I GTSAC 2 cut(s) 2402, 3105
TspGWI ACGGA 2 cut(s) 3143, 3204
TspRI CASTG 7 cut(s) 210, 1183, 1794, 2167, 2893, 3643, 3748
Vha464I CTTAAG 1 cut(s) 2717
VpaK11BI GGWCC 1 cut(s) 2648
VspI ATTAAT 1 cut(s) 1049
XapI RAATTY 6 cut(s) 1973, 2471, 2681, 3146, 3670, 3685
XbaI TCTAGA 1 cut(s) 3118
XceI RCATGY 5 cut(s) 1565, 1819, 2111, 3078, 3184
XcmI CCANNNNNNNNNTGG 3 cut(s) 1021, 1844, 3404
XhoI CTCGAG 2 cut(s) 8, 2174
XmnI GAANNNNTTC 2 cut(s) 2874, 2952
XspI CTAG 6 cut(s) 81, 959, 978, 1367, 2945, 3119
ZrmI AGTACT 2 cut(s) 2606, 2874
Zsp2I ATGCAT 3 cut(s) 136, 508, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.