Rh2AG508900

Fanconi anaemia protein FancD2 nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006206
Physical Location & Seq
Reverse (-)
73361881 .. 73370902
9022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG508900.1

Sequence Viewer

Length: 3930 bp
ATGGTGTTCCTCCACCAACAACTCCCTTCCCGCAAGAGACCCGCCTCTTCTCTCGGCCCCCCTCCCCCTCCCCCCAAATCCTCAAAACCCAAAACCACCGCCACCGCCGCCTCAAATTCAACACAAAACGACGCCGTCGATACCCCCGCCGTCGATAAAATGGTCTCAATTTTAGCCGAGGCCGGTTGCACCCTCGTCAACCCCTCCGGCCCTCCGTGCCTCCCCTCCGATCCTCACAAGCTCCGCCGCGATCTCCACCGCACATTTTCCTCTTCCGAAAATGCCCCTGCTCTCCGCTCCGATTTCCTCAGCGGTCTCTCCTCCTACATTCAAACCCCCAGAAACCTCCGCAGGGTTCTTACTGCTTCAACCAGAACCGAGAGCTTACTCCGGCACCTATTGCTGGTCCCGTCGATCCAAGTCGACATTCAGAACATGCTTCTCGAGAAGCTTCCGGAGTATTTCCATGTGGATTCGGATCAGAATCCATCGCTTTCTCTTGAAGATGATGTTGCTAGGCTTATAATCAACCACTTCCGGTGGCTCGATTTCGTTGTTGATTCTAATGCATTGGCTGAGAAATTGATGCAAGTGCTCTCGATTTGCCCTTTGCATTTGAAGAAGGAGATCATTGGATCACTGCCGGAGATTATTGGGGATCAGGATAACAAGACTGTGGTTCAGTCTCTGGAGAAAATGCTTGAAGAGGACTCGGCTGTAGTTGTGGCTGTGCTTGATTCCTTCTCGAACCTTAATTTGGATAATGAGTTGCAAGAACAGGTTATCACCATAGCATTGTCCTGCATCAGAACAATTGATGCGGAGCATATGCCCTACCTGCTTAGATTTTTATTGCTTTCAGCAACACCATCGAATGTAAGGCGAATAATCTCTCAGATACGTGCGAAGCTGAAGTTTGTTGGTGTATCAAGCCATCATGCATCACAACAGAGTAAACTCAAAGGAAAGTCTCGGGTGGACAACACAGAGGCTTCTATTCTTGATGCCTTGAGATCAAGTCTTAGATTTAAGAATATACTGTGTCAGGAGATCTTGAAAGACTTGAACTGTCTTGAGAAACCTCAGGATCACAAAGTCATTGACATATGGCTACTTATGCTGATATACATGAATGGCGAATCTTTGCAAAGGAGTGTTGAAAAGCTATTTAAGAAAAAAGTTATTGAAGATTGTATTCAGGAAGCTATGTTTGATCAATGCATTCATGGGCACAAGGAGCTCGTACAGGATTATTTTCTATCTTTCATTTCTTTGTCTGAGTACCTGTTGGCTTGCAAAGAGCAGAAGGCCAGGCACTTTAGCATTCATATGTATGTTTGTCTCTTTGAGGAGTTTGATGATACTTATTCAAGACAGGAAGTTCTTGGTTCACTAGTTACCCATGTGGGCTCTAGTGTGGGTTTTGAAGTCAGTTCTTCTTTAGAGACCATGAGGTTGCTGGTTTCCAAATATGCACAGCAATTAATTTCTCTTTCTTCTCATATTAGTGGTATCTTGGATTATTTAGAGGGATTCAGTCTTGAAAATCTGCACAAGGTCTACGAGATTTTCAGCCATCTGGCCCTTTTGGCTCGGTCAAGTGTAGATTCATATGGTTCTTCCTTTGCAAATGAACTTTTCATGATAGTACGTAAGCAGGTTAGTCATCCAGATCTGAACTACAAGAAGATGGGCCTTGTTGGGACTCTGAAGATAGTTTCCTGTCTAGGATATGCAGCTAATGATACATATCCATCCCCATCTCAGAAATCAAACTGTGATGAGGCTCTGGAACTCTTGAAAACAGCTTTGGACTCTTGCAAACAGTTGCCCTTACCTCTGATCATGTTTTATGACGAACTGACTGAAATGATGGATAATAACACACTTGATCCAATAGTTATGGAATGGATTGGCAAACATGTAGGAGAGTTTGAATCTATATTTCTTTCTGATCTTGACGGTGGGAAATTAGCAGCCAACGAATCTTATTGTGGTTTAGAAGGTGAACTGTGGATGAACTTGGACGGTGATATATCTCCTATTTGTCTAAACATTTTGCCATTGGCTTCATCTACATTGCAGTCTGCTTCTTCTTTACAAGTTCTTCCTGCTAACTTCCTTCTATTATCTGCAATTGAAAGGTTGACAAATCAAGGATCTCTTGGTGGAATTGATGCACTACTGGGCTGTCCTCTCCACCTCCCTTCTTCAAAGTATTTCTTTGGATCTGAGTGGCCGTCTCTCACAGGGAAGCAGAAACAGATTGTATGTGGCTCTCTATTTTATGCTTCTAATTGGATACGGGAACTACTCAATGCCTTCTGTACACAAATCACCGGAAGATTTGAATTTACAAGTCAGGCTACTAAGGAGGACATAATTGCCAAGCTTTTGAAGCGTGTGAGGAATCTTATATTTTTGGAAAGCTTATTAAACAATTGTATTGAGAGGTACTCTCTACCTCTTCCTGAACTTCATCCACATGCGGATATCAATCAGTCTTCTCTCTTAAACCAACCTCACCATATGAGGCACACTGAAAAGAAACTCGAGCATAAGAAGACACAAGAGGACATCTCTCCAAGTGGCGCGAGGAGAAATAATAAGACCTCAAAAGCATCAACAACATCAGATACTATGGGAAAACTCCGGCAGCCCACATTATTTGATGTCTTGGAGAAAGTGGGAGTTATAACAGGCCAAGAGGTGCCAAATGAGGATTCTTCCAGTTTATCTACAAAGGGAAGGTCATCTGAGTCATCTCAGAAAAATCCCTGTGACTTTAATGAGGCTGCCATTATAGAAATGTCTGCTGTTGCCAAGGCCCTTGAAGCTCAGAGATCTAAATTCAGACCTCTCCTGGTTCAATGTTATTCAGTTTTAGCAATTTCAAAGAGTGAAGCGTCCTGTTGCTCTGATGCTGCAGCTGAGTTACCCCTACATCTGTACCTTCTGCGTGATCTGCACAACAAGCTGGAGTACTTCAATCCTGGAAAACAGTTGTGGGGTAAATGCTTGAATGGTCCAGTTGGGTTCACAAGGATGACATTAGATGAATTCGTGAGCAGAATTGAACCACTCTTTCCAAGTCTTAAGAAACATTTGGATAGTGCAGTTTGGTTACTTAAAGAAGGTGATGAAACTTGTGAAGAACATTGGAATAATGAATCTACTGCTGCTGGAAATCCAGACATTCCCAATCTTGTACTTTCAAACTACACAGCTTCTACTTCAGTATTCAAAGAAGTACTTCATTGTTTCAGTGCGATATTAAACCTTCCTGGTCTTCAAACAGACAAGCCAGCTCTCTCACACCTGCTAGAAGCATTTCAGCCCGATAAGATTCCAGATGGTTTTTTTGCAGGGATACAGCCTAACCTTTCTCCTGGGACTGCAGAATATTTATATTTTGGAGCTTATTCATTCTTTGAAGGTGTCTTGGACATAGCATGTTCTTTCTCCTTTATGCTGGCTTCAGAGTCACTTTTTACTCTAGAATCAGTTGTAACCTCAGTCCAGAAATTTGTCAGTAAGTTGGAGGGAAATGGTAAAAGCATGCAGTACGGATTTATCCAGGAGGCTGTTCCTACTTTGCGCAGAAAACTTGGTACTTCTGCGGAGAAGATTTTAAGGCATAGATGGGATGATGACAATCTTGATAATGGTTGGAAGAGCAAAGGAGAAACAGTACAGAAGATATTGCGTATTTACCTGGATAATAGCGGCTCTACTCCTGATCTGCTGGATGAGCTCTCCTGCTCTATTTTACCCCAGGCTTCTTTTGGAAAAGGAAAGGAAGAAGATGATCGTCATGGTTTTCCTTCTCTATCTTCTGGAACATTTGGTGTATGGTACAAGATATTGGTAAGACTATGGTTTTGCTTTATTTATGATGGTTTTCTTGTTGGAGATGTTTACACAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FancD2

Protein Analysis

1309

Amino Acids

146.21

Weight (kDa)

5.78

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FancD2 PF14631 27 - 876 1.7e-127 Fanconi anaemia protein FancD2 nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 524, 2696
AarI CACCTGC 1 cut(s) 3327
Acc16I TGCGCA 1 cut(s) 3599
Acc36I ACCTGC 3 cut(s) 846, 1648, 3327
AccB1I GGYRCC 2 cut(s) 393, 2710
AccBSI CCGCTC 1 cut(s) 297
AccI GTMKAC 2 cut(s) 423, 1560
AccII CGCG 2 cut(s) 249, 2594
AccIII TCCGGA 1 cut(s) 454
AclWI GGATC 9 cut(s) 224, 409, 486, 643, 666, 1095, 1886, 2167, 2236
AcoI YGGCCR 1 cut(s) 2237
AcsI RAATTY 5 cut(s) 115, 2351, 2849, 3059, 3524
AcuI CTGAAG 4 cut(s) 932, 1730, 3219, 3462
AcyI GRCGYC 1 cut(s) 132
AdeI CACNNNGTG 1 cut(s) 3927
AfiI CCNNNNNNNGG 3 cut(s) 352, 403, 757
AflII CTTAAG 1 cut(s) 3095
AflIII ACRYGT 1 cut(s) 1921
AhlI ACTAGT 1 cut(s) 1393
AjnI CCWGG 8 cut(s) 1310, 2862, 2992, 3283, 3388, 3576, 3714, 3774
AjuI GAANNNNNNNTTGG 8 cut(s) 411, 443, 740, 772, 1793, 1825, 2707, 2739
Alw21I GWGCWC 3 cut(s) 597, 1242, 3756
Alw26I GTCTC 8 cut(s) 31, 169, 320, 690, 975, 1346, 1439, 2247
AlwI GGATC 9 cut(s) 224, 409, 486, 643, 666, 1095, 1886, 2167, 2236
AlwNI CAGNNNCTG 1 cut(s) 688
Ama87I CYCGRG 3 cut(s) 443, 972, 2552
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 9 cut(s) 55, 180, 208, 1308, 1581, 1693, 2237, 2701, 2826
ApeKI GCWGC 7 cut(s) 1736, 1976, 2656, 2795, 2924, 2927, 3179
ApoI RAATTY 5 cut(s) 115, 2351, 2849, 3059, 3524
ArsI GACNNNNNNTTYG 4 cut(s) 2735, 2767, 3864, 3896
AseI ATTAAT 1 cut(s) 1484
Asp700I GAANNNNTTC 2 cut(s) 3252, 3330
AspLEI GCGC 2 cut(s) 2594, 3600
AspS9I GGNCC 7 cut(s) 56, 209, 406, 1582, 1693, 2827, 3026
AsuHPI GGTGA 6 cut(s) 778, 2018, 2042, 2329, 2516, 3149
AvaI CYCGRG 3 cut(s) 443, 972, 2552
AvaII GGWCC 2 cut(s) 406, 3026
AxyI CCTNAGG 1 cut(s) 1083
BaeGI GKGCMC 1 cut(s) 1233
BaeI ACNNNNGTAYC 2 cut(s) 2933, 2966
BanI GGYRCC 2 cut(s) 393, 2710
BanII GRGCYC 3 cut(s) 1242, 1412, 3756
BbsI GAAGAC 3 cut(s) 2496, 2570, 3281
Bbv12I GWGCWC 3 cut(s) 597, 1242, 3756
BbvCI CCTCAGC 1 cut(s) 308
BbvI GCAGC 7 cut(s) 1748, 1988, 2668, 2782, 2911, 2939, 3166
BceAI ACGGC 3 cut(s) 119, 134, 2224
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
BciVI GTATCC 2 cut(s) 2295, 3363
BclI TGATCA 2 cut(s) 1213, 1842
BcoDI GTCTC 8 cut(s) 31, 169, 320, 690, 975, 1346, 1439, 2247
BcuI ACTAGT 1 cut(s) 1393
BfaI CTAG 6 cut(s) 516, 1394, 1413, 1727, 3323, 3497
BfmI CTRYAG 3 cut(s) 717, 2925, 3396
BfrI CTTAAG 1 cut(s) 3095
BfuAI ACCTGC 3 cut(s) 846, 1648, 3327
BfuI GTATCC 2 cut(s) 2295, 3363
BglI GCCNNNNNGGC 1 cut(s) 1589
BglII AGATCT 3 cut(s) 1050, 1672, 2843
BmcAI AGTACT 2 cut(s) 2984, 3252
Bme1390I CCNGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
Bme18I GGWCC 2 cut(s) 406, 3026
BmeT110I CYCGRG 3 cut(s) 443, 972, 2552
BmgT120I GGNCC 7 cut(s) 56, 209, 406, 1582, 1693, 2827, 3026
BmiI GGNNCC 4 cut(s) 58, 395, 408, 2712
BmrFI CCNGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
BmrI ACTGGG 1 cut(s) 2195
BmsI GCATC 8 cut(s) 576, 808, 813, 950, 994, 2167, 2630, 2911
BmuI ACTGGG 1 cut(s) 2195
BpiI GAAGAC 3 cut(s) 2496, 2570, 3281
BplI GAGNNNNNCTC 6 cut(s) 2441, 2473, 2443, 2475, 2564, 2596
BpmI CTGGAG 2 cut(s) 710, 2999
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 2 cut(s) 1030, 1094
BsaAI YACGTR 2 cut(s) 902, 1652
BsaBI GATNNNNATC 5 cut(s) 477, 483, 1749, 2496, 3654
BsaHI GRCGYC 1 cut(s) 132
BsaI GGTCTC 4 cut(s) 31, 169, 320, 1439
BsaJI CCNNGG 4 cut(s) 177, 2823, 3389, 3774
BsaWI WCCGGW 3 cut(s) 454, 537, 2339
BsaXI ACNNNNNCTCC 2 cut(s) 3675, 3705
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 403, 757
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 2190, 2730, 3029
Bse21I CCTNAGG 1 cut(s) 1083
Bse3DI GCAATG 1 cut(s) 2078
Bse8I GATNNNNATC 5 cut(s) 477, 483, 1749, 2496, 3654
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
BseDI CCNNGG 4 cut(s) 177, 2823, 3389, 3774
BseGI GGATG 7 cut(s) 1666, 1754, 2022, 2479, 3051, 3652, 3754
BseJI GATNNNNATC 5 cut(s) 477, 483, 1749, 2496, 3654
BseLI CCNNNNNNNGG 3 cut(s) 352, 403, 757
BseMI GCAATG 1 cut(s) 2078
BseNI ACTGG 3 cut(s) 2190, 2730, 3029
BseRI GAGGAG 3 cut(s) 310, 1364, 2611
BseSI GKGCMC 1 cut(s) 1233
BseXI GCAGC 7 cut(s) 1748, 1988, 2668, 2782, 2911, 2939, 3166
BsgI GTGCAG 3 cut(s) 1535, 2951, 3135
Bsh1236I CGCG 2 cut(s) 249, 2594
BshFI GGCC 9 cut(s) 57, 182, 210, 1310, 1583, 1695, 2239, 2703, 2828
BshNI GGYRCC 2 cut(s) 393, 2710
BsiHKAI GWGCWC 3 cut(s) 597, 1242, 3756
BsiHKCI CYCGRG 3 cut(s) 443, 972, 2552
BsiSI CCGG 8 cut(s) 183, 207, 391, 455, 538, 644, 2340, 2653
BslFI GGGAC 3 cut(s) 392, 1717, 3406
BslI CCNNNNNNNGG 3 cut(s) 352, 403, 757
BsmAI GTCTC 8 cut(s) 31, 169, 320, 690, 975, 1346, 1439, 2247
BsmBI CGTCTC 1 cut(s) 2247
BsmFI GGGAC 3 cut(s) 392, 1717, 3406
BsmI GAATGC 2 cut(s) 1221, 1323
BsnI GGCC 9 cut(s) 57, 182, 210, 1310, 1583, 1695, 2239, 2703, 2828
Bso31I GGTCTC 4 cut(s) 31, 169, 320, 1439
BsoBI CYCGRG 3 cut(s) 443, 972, 2552
Bsp1286I GDGCHC 5 cut(s) 597, 1233, 1242, 1412, 3756
Bsp13I TCCGGA 1 cut(s) 454
Bsp1407I TGTACA 1 cut(s) 2327
BspANI GGCC 9 cut(s) 57, 182, 210, 1310, 1583, 1695, 2239, 2703, 2828
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 2 cut(s) 249, 2594
BspHI TCATGA 1 cut(s) 1641
BspLI GGNNCC 4 cut(s) 58, 395, 408, 2712
BspMAI CTGCAG 2 cut(s) 2929, 3400
BspMI ACCTGC 3 cut(s) 846, 1648, 3327
BspPI GGATC 9 cut(s) 224, 409, 486, 643, 666, 1095, 1886, 2167, 2236
BspQI GCTCTTC 1 cut(s) 3668
BspT107I GGYRCC 2 cut(s) 393, 2710
BspTI CTTAAG 1 cut(s) 3095
BspTNI GGTCTC 4 cut(s) 31, 169, 320, 1439
BsrBI CCGCTC 1 cut(s) 297
BsrDI GCAATG 1 cut(s) 2078
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 2327
BsrI ACTGG 3 cut(s) 2190, 2730, 3029
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 4 cut(s) 177, 2823, 3389, 3774
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 2823
Bst2UI CCWGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
Bst6I CTCTTC 5 cut(s) 52, 277, 699, 2472, 3668
BstACI GRCGYC 1 cut(s) 132
BstAFI CTTAAG 1 cut(s) 3095
BstAPI GCANNNNNTGC 1 cut(s) 400
BstAUI TGTACA 1 cut(s) 2327
BstBAI YACGTR 2 cut(s) 902, 1652
BstC8I GCNNGC 4 cut(s) 1294, 3306, 3474, 3560
BstF5I GGATG 7 cut(s) 1666, 1754, 2022, 2479, 3051, 3652, 3754
BstFNI CGCG 2 cut(s) 249, 2594
BstHHI GCGC 2 cut(s) 2594, 3600
BstMAI GTCTC 8 cut(s) 31, 169, 320, 690, 975, 1346, 1439, 2247
BstNI CCWGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
BstNSI RCATGY 5 cut(s) 439, 1925, 2489, 3456, 3562
BstSCI CCNGG 8 cut(s) 1310, 2862, 2992, 3283, 3388, 3576, 3714, 3774
BstSFI CTRYAG 3 cut(s) 717, 2925, 3396
BstSLI GKGCMC 1 cut(s) 1233
BstSNI TACGTA 1 cut(s) 1652
BstUI CGCG 2 cut(s) 249, 2594
BstV1I GCAGC 7 cut(s) 1748, 1988, 2668, 2782, 2911, 2939, 3166
BstV2I GAAGAC 3 cut(s) 2496, 2570, 3281
BstX2I RGATCY 5 cut(s) 1050, 1672, 2159, 2228, 2843
BstYI RGATCY 5 cut(s) 1050, 1672, 2159, 2228, 2843
Bsu36I CCTNAGG 1 cut(s) 1083
BsuI GTATCC 2 cut(s) 2295, 3363
BsuRI GGCC 9 cut(s) 57, 182, 210, 1310, 1583, 1695, 2239, 2703, 2828
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 7 cut(s) 1666, 1754, 2022, 2479, 3051, 3652, 3754
BtsI GCAGTG 1 cut(s) 638
BtsIMutI CAGTG 3 cut(s) 638, 2538, 3271
BveI ACCTGC 3 cut(s) 846, 1648, 3327
Cac8I GCNNGC 4 cut(s) 1294, 3306, 3474, 3560
CaiI CAGNNNCTG 1 cut(s) 688
CciI TCATGA 1 cut(s) 1641
CfoI GCGC 2 cut(s) 2594, 3600
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 7 cut(s) 56, 209, 406, 1582, 1693, 2827, 3026
CseI GACGC 2 cut(s) 140, 2895
CspCI CAANNNNNGTGG 2 cut(s) 2650, 2685
DraIII CACNNNGTG 1 cut(s) 3927
EaeI YGGCCR 1 cut(s) 2237
Eam1104I CTCTTC 5 cut(s) 52, 277, 699, 2472, 3668
EarI CTCTTC 5 cut(s) 52, 277, 699, 2472, 3668
EciI GGCGGA 1 cut(s) 233
Ecl136II GAGCTC 2 cut(s) 1240, 3754
Eco105I TACGTA 1 cut(s) 1652
Eco130I CCWWGG 1 cut(s) 2823
Eco24I GRGCYC 3 cut(s) 1242, 1412, 3756
Eco31I GGTCTC 4 cut(s) 31, 169, 320, 1439
Eco32I GATATC 1 cut(s) 2494
Eco47I GGWCC 2 cut(s) 406, 3026
Eco53kI GAGCTC 2 cut(s) 1240, 3754
Eco57I CTGAAG 4 cut(s) 932, 1730, 3219, 3462
Eco81I CCTNAGG 1 cut(s) 1083
Eco88I CYCGRG 3 cut(s) 443, 972, 2552
EcoICRI GAGCTC 2 cut(s) 1240, 3754
EcoO109I RGGNCCY 1 cut(s) 2827
EcoRI GAATTC 1 cut(s) 3059
EcoRII CCWGG 8 cut(s) 1310, 2862, 2992, 3283, 3388, 3576, 3714, 3774
EcoRV GATATC 1 cut(s) 2494
EcoT14I CCWWGG 1 cut(s) 2823
EcoT22I ATGCAT 3 cut(s) 571, 943, 1223
EcoT38I GRGCYC 3 cut(s) 1242, 1412, 3756
ErhI CCWWGG 1 cut(s) 2823
Esp3I CGTCTC 1 cut(s) 2247
FalI AAGNNNNNCTT 6 cut(s) 2148, 2180, 2207, 2239, 3237, 3269
FaqI GGGAC 3 cut(s) 392, 1717, 3406
FauI CCCGC 3 cut(s) 38, 49, 154
FauNDI CATATG 5 cut(s) 828, 1106, 1329, 1612, 2529
FbaI TGATCA 2 cut(s) 1213, 1842
FblI GTMKAC 2 cut(s) 423, 1560
FokI GGATG 7 cut(s) 1653, 1741, 2029, 2466, 3058, 3659, 3761
FriOI GRGCYC 3 cut(s) 1242, 1412, 3756
FspBI CTAG 6 cut(s) 516, 1394, 1413, 1727, 3323, 3497
FspI TGCGCA 1 cut(s) 3599
GlaI GCGC 2 cut(s) 2593, 3599
GsuI CTGGAG 2 cut(s) 710, 2999
HaeIII GGCC 9 cut(s) 57, 182, 210, 1310, 1583, 1695, 2239, 2703, 2828
HapII CCGG 8 cut(s) 183, 207, 391, 455, 538, 644, 2340, 2653
HgaI GACGC 2 cut(s) 140, 2895
HhaI GCGC 2 cut(s) 2594, 3600
Hin1I GRCGYC 1 cut(s) 132
Hin6I GCGC 2 cut(s) 2592, 3598
HinP1I GCGC 2 cut(s) 2592, 3598
HincII GTYRAC 3 cut(s) 199, 424, 2148
HindII GTYRAC 3 cut(s) 199, 424, 2148
HindIII AAGCTT 3 cut(s) 449, 2390, 2428
HpaII CCGG 8 cut(s) 183, 207, 391, 455, 538, 644, 2340, 2653
HphI GGTGA 6 cut(s) 778, 2018, 2042, 2329, 2516, 3149
Hpy99I CGWCG 4 cut(s) 134, 140, 155, 415
HpyCH4IV ACGT 2 cut(s) 901, 1651
HpySE526I ACGT 2 cut(s) 901, 1651
Hsp92I GRCGYC 1 cut(s) 132
HspAI GCGC 2 cut(s) 2592, 3598
Kpn2I TCCGGA 1 cut(s) 454
Ksp22I TGATCA 2 cut(s) 1213, 1842
LguI GCTCTTC 1 cut(s) 3668
LmnI GCTCC 5 cut(s) 246, 302, 823, 1237, 3416
Lsp1109I GCAGC 7 cut(s) 1748, 1988, 2668, 2782, 2911, 2939, 3166
LweI GCATC 8 cut(s) 576, 808, 813, 950, 994, 2167, 2630, 2911
MaeI CTAG 6 cut(s) 516, 1394, 1413, 1727, 3323, 3497
MaeII ACGT 2 cut(s) 901, 1651
MaeIII GTNAC 6 cut(s) 1396, 2780, 2934, 3123, 3483, 3508
MbiI CCGCTC 1 cut(s) 297
MfeI CAATTG 3 cut(s) 813, 2136, 2440
MflI RGATCY 5 cut(s) 1050, 1672, 2159, 2228, 2843
MhlI GDGCHC 5 cut(s) 597, 1233, 1242, 1412, 3756
MlyI GAGTC 5 cut(s) 704, 1699, 1808, 2768, 3491
MmeI TCCRAC 3 cut(s) 3519, 3650, 3889
Mph1103I ATGCAT 3 cut(s) 571, 943, 1223
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 3252, 3330
MslI CAYNNNNRTG 5 cut(s) 1328, 1332, 1506, 2484, 3044
MspA1I CMGCKG 2 cut(s) 312, 2930
MspCI CTTAAG 1 cut(s) 3095
MspI CCGG 8 cut(s) 183, 207, 391, 455, 538, 644, 2340, 2653
MspR9I CCNGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
MunI CAATTG 3 cut(s) 813, 2136, 2440
Mva1269I GAATGC 2 cut(s) 1221, 1323
MvaI CCWGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
MvnI CGCG 2 cut(s) 249, 2594
NdeI CATATG 5 cut(s) 828, 1106, 1329, 1612, 2529
NlaIV GGNNCC 4 cut(s) 58, 395, 408, 2712
NmeAIII GCCGAG 3 cut(s) 33, 202, 692
NmuCI GTSAC 2 cut(s) 2780, 3483
NsbI TGCGCA 1 cut(s) 3599
NsiI ATGCAT 3 cut(s) 571, 943, 1223
NspI RCATGY 5 cut(s) 439, 1925, 2489, 3456, 3562
PaeI GCATGC 1 cut(s) 3562
PaeR7I CTCGAG 2 cut(s) 443, 2552
PagI TCATGA 1 cut(s) 1641
PaqCI CACCTGC 1 cut(s) 3327
PciI ACATGT 1 cut(s) 1921
PciSI GCTCTTC 1 cut(s) 3668
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 2 cut(s) 1221, 1323
PdmI GAANNNNTTC 2 cut(s) 3252, 3330
PflFI GACNNNGTC 1 cut(s) 134
PfoI TCCNGGA 2 cut(s) 2992, 3576
PleI GAGTC 5 cut(s) 704, 1699, 1808, 2767, 3490
PpsI GAGTC 5 cut(s) 704, 1699, 1808, 2767, 3490
Ppu21I YACGTR 2 cut(s) 902, 1652
PscI ACATGT 1 cut(s) 1921
PshBI ATTAAT 1 cut(s) 1484
PsiI TTATAA 2 cut(s) 524, 2696
Psp124BI GAGCTC 2 cut(s) 1242, 3756
Psp6I CCWGG 8 cut(s) 1310, 2862, 2992, 3283, 3388, 3576, 3714, 3774
PspGI CCWGG 8 cut(s) 1310, 2862, 2992, 3283, 3388, 3576, 3714, 3774
PspN4I GGNNCC 4 cut(s) 58, 395, 408, 2712
PspPI GGNCC 7 cut(s) 56, 209, 406, 1582, 1693, 2827, 3026
PspXI VCTCGAGB 1 cut(s) 2552
PstI CTGCAG 2 cut(s) 2929, 3400
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 5 cut(s) 1050, 1672, 2159, 2228, 2843
PsyI GACNNNGTC 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 2930
RseI CAYNNNNRTG 5 cut(s) 1328, 1332, 1506, 2484, 3044
SacI GAGCTC 2 cut(s) 1242, 3756
SalI GTCGAC 1 cut(s) 422
SapI GCTCTTC 1 cut(s) 3668
Sau96I GGNCC 7 cut(s) 56, 209, 406, 1582, 1693, 2827, 3026
ScaI AGTACT 2 cut(s) 2984, 3252
SchI GAGTC 5 cut(s) 704, 1699, 1808, 2768, 3491
ScrFI CCNGG 8 cut(s) 1312, 2864, 2994, 3285, 3390, 3578, 3716, 3776
SduI GDGCHC 5 cut(s) 597, 1233, 1242, 1412, 3756
SfaNI GCATC 8 cut(s) 576, 808, 813, 950, 994, 2167, 2630, 2911
SfcI CTRYAG 3 cut(s) 717, 2925, 3396
Sfr274I CTCGAG 2 cut(s) 443, 2552
SinI GGWCC 2 cut(s) 406, 3026
SlaI CTCGAG 2 cut(s) 443, 2552
SmiMI CAYNNNNRTG 5 cut(s) 1328, 1332, 1506, 2484, 3044
SmlI CTYRAG 5 cut(s) 443, 1009, 1073, 2552, 3095
SmoI CTYRAG 5 cut(s) 443, 1009, 1073, 2552, 3095
SnaBI TACGTA 1 cut(s) 1652
SpeI ACTAGT 1 cut(s) 1393
SphI GCATGC 1 cut(s) 3562
SspI AATATT 1 cut(s) 3404
SspMI CTAG 6 cut(s) 516, 1394, 1413, 1727, 3323, 3497
SstI GAGCTC 2 cut(s) 1242, 3756
StyD4I CCNGG 8 cut(s) 1310, 2862, 2992, 3283, 3388, 3576, 3714, 3774
StyI CCWWGG 1 cut(s) 2823
TaiI ACGT 2 cut(s) 904, 1654
TaqII GACCGA 1 cut(s) 1584
TatI WGTACW 5 cut(s) 2327, 2982, 3208, 3250, 3691
TauI GCSGC 3 cut(s) 110, 249, 3729
TscAI CASTG 3 cut(s) 645, 2545, 3271
TseFI GTSAC 2 cut(s) 2780, 3483
TseI GCWGC 7 cut(s) 1736, 1976, 2656, 2795, 2924, 2927, 3179
Tsp45I GTSAC 2 cut(s) 2780, 3483
TspGWI ACGGA 2 cut(s) 204, 3582
TspRI CASTG 3 cut(s) 645, 2545, 3271
Tth111I GACNNNGTC 1 cut(s) 134
Vha464I CTTAAG 1 cut(s) 3095
VpaK11BI GGWCC 2 cut(s) 406, 3026
VspI ATTAAT 1 cut(s) 1484
XapI RAATTY 5 cut(s) 115, 2351, 2849, 3059, 3524
XbaI TCTAGA 1 cut(s) 3496
XceI RCATGY 5 cut(s) 439, 1925, 2489, 3456, 3562
XcmI CCANNNNNNNNNTGG 2 cut(s) 1456, 3782
XhoI CTCGAG 2 cut(s) 443, 2552
XmiI GTMKAC 2 cut(s) 423, 1560
XmnI GAANNNNTTC 2 cut(s) 3252, 3330
XspI CTAG 6 cut(s) 516, 1394, 1413, 1727, 3323, 3497
ZrmI AGTACT 2 cut(s) 2984, 3252
Zsp2I ATGCAT 3 cut(s) 571, 943, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.