Rmu_sc0003819.1_g000012

elf4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003819.1
OG0011811
Physical Location & Seq
Forward (+)
42260 .. 42610
351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003819.1_g000012.1.cds

Sequence Viewer

Length: 351 bp
atggaaggagacacattttctgggctagttggcaatggcacccaaattgatggcaaagtgttgcagacatttcagaagagctttgttcaagtccaggatatcttggaccagaacaggttgctcataaatgagataaaccaaaaccatgagtctaagatccctgacaacctgagcagaaatgtgggtctgatcagagagctcaacgacaatatcaggagggtggtagacctctatgctgatctctcaagctccttcaccaaatccatggagacttcatctgagggagactccagcggtggtgctttgaaatcagatggtaaagctgggcacaagaggaataggccttcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1313

Protein Analysis

116

Amino Acids

12.75

Weight (kDa)

5.62

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 225
AciI CCGC 1 cut(s) 294
AclWI GGATC 1 cut(s) 151
AgsI TTSAA 2 cut(s) 89, 307
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 4 cut(s) 81, 199, 249, 323
AluI AGCT 4 cut(s) 81, 199, 249, 323
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 3 cut(s) 3, 263, 279
AlwI GGATC 1 cut(s) 151
AoxI GGCC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 330
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BccI CCATC 2 cut(s) 44, 308
BciT130I CCWGG 1 cut(s) 95
BclI TGATCA 1 cut(s) 189
BcoDI GTCTC 3 cut(s) 3, 263, 279
BfaI CTAG 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 95
BplI GAGNNNNNCTC 2 cut(s) 272, 304
BpmI CTGGAG 1 cut(s) 274
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 229
BsaJI CCNNGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 40
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 2 cut(s) 161, 270
BseSI GKGCMC 1 cut(s) 330
BseYI CCCAGC 1 cut(s) 323
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 201
BsmAI GTCTC 3 cut(s) 3, 263, 279
BsnI GGCC 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 201, 330
Bsp143I GATC 3 cut(s) 156, 189, 238
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 1 cut(s) 294
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 2 cut(s) 162, 271
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 40
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 3 cut(s) 156, 189, 238
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 95
Bst6I CTCTTC 1 cut(s) 71
BstDEI CTNAG 3 cut(s) 153, 170, 279
BstDSI CCRYGG 1 cut(s) 264
BstKTI GATC 3 cut(s) 159, 192, 241
BstMAI GTCTC 3 cut(s) 3, 263, 279
BstMBI GATC 3 cut(s) 156, 189, 238
BstNI CCWGG 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 330
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 2 cut(s) 50, 265
BstYI RGATCY 1 cut(s) 156
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 106
CviAII CATG 2 cut(s) 146, 265
CviJI RGCY 6 cut(s) 25, 81, 199, 249, 323, 343
CviKI_1 RGCY 6 cut(s) 25, 81, 199, 249, 323, 343
DdeI CTNAG 3 cut(s) 153, 170, 279
DpnI GATC 3 cut(s) 158, 191, 240
DpnII GATC 3 cut(s) 156, 189, 238
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 264
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 93
EcoRV GATATC 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 2 cut(s) 149, 268
FaiI YATR 5 cut(s) 125, 147, 234, 266, 349
FatI CATG 2 cut(s) 145, 264
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 1 cut(s) 225
FriOI GRGCYC 1 cut(s) 201
FspBI CTAG 1 cut(s) 26
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 1 cut(s) 343
Hin1II CATG 2 cut(s) 149, 268
HinfI GANTC 2 cut(s) 149, 287
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 5 cut(s) 75, 189, 194, 280, 313
Hpy188III TCNNGA 1 cut(s) 214
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 262
HpyCH4V TGCA 1 cut(s) 64
HpyF3I CTNAG 3 cut(s) 153, 170, 279
Hsp92II CATG 2 cut(s) 149, 268
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 3 cut(s) 156, 189, 238
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 254
MaeI CTAG 1 cut(s) 26
MalI GATC 3 cut(s) 158, 191, 240
MboI GATC 3 cut(s) 156, 189, 238
MboII GAAGA 1 cut(s) 88
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 201, 330
MluCI AATT 1 cut(s) 45
MlyI GAGTC 2 cut(s) 158, 281
MnlI CCTC 4 cut(s) 210, 239, 274, 327
MspA1I CMGCKG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
NcoI CCATGG 1 cut(s) 264
NdeII GATC 3 cut(s) 156, 189, 238
NlaIII CATG 2 cut(s) 149, 268
NlaIV GGNNCC 1 cut(s) 40
PceI AGGCCT 1 cut(s) 343
PciSI GCTCTTC 1 cut(s) 71
PfoI TCCNGGA 1 cut(s) 93
PleI GAGTC 2 cut(s) 157, 281
PpsI GAGTC 2 cut(s) 157, 281
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 156
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 71
Sau3AI GATC 3 cut(s) 156, 189, 238
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 2 cut(s) 158, 281
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 2 cut(s) 201, 330
SetI ASST 7 cut(s) 83, 119, 171, 201, 231, 251, 325
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
Sse9I AATT 1 cut(s) 45
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 1 cut(s) 294
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 201
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 1 cut(s) 93
StyI CCWWGG 1 cut(s) 264
TasI AATT 1 cut(s) 45
TspDTI ATGAA 2 cut(s) 264, 336
VpaK11BI GGWCC 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.