Rmu_sc0004135.1_g000003

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004135.1
OG0019513
Physical Location & Seq
Forward (+)
8014 .. 9039
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004135.1_g000003.1.cds

Sequence Viewer

Length: 396 bp
atgctcgaggaagatgctttgctgcgcgcttttgctcaccatgtcagctttgtcacatctgccaagcatgttgaacctttaggtctgctagcaggtttgcagatgttgcaaacaagacaaattacagattgtcttgttgaaacagattgccttgtggctgtatctgaaattcagttggctgactatcaaaatctggagtatggaaaccttattgatgatataaaagtgtggctcgatggagcagtgcttcggtttgcggcggagactctggtggacaaggccatggagctcgaccacatcggcgggacctttggaggtaatagaaaacccacaccgttcatttatgaagatgcttcagatgcaacagaagaaggaaattgtcatcgaattcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

131

Amino Acids

14.53

Weight (kDa)

4.58

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019513)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004135.1_g000003
rosa_rugosa Rorug03G0316400
rosa_samantha Rh2BG035600 Rh5BG425300 Rh5CG449400 Rh6AG240200 Rh6BG084600 Rh7AG481100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AccII CGCG 1 cut(s) 27
AciI CCGC 3 cut(s) 257, 260, 303
AcsI RAATTY 2 cut(s) 168, 387
AcuI CTGAAG 1 cut(s) 339
AgsI TTSAA 2 cut(s) 74, 140
AluBI AGCT 2 cut(s) 48, 289
AluI AGCT 2 cut(s) 48, 289
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 5
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 22
ApoI RAATTY 2 cut(s) 168, 387
AspLEI GCGC 2 cut(s) 27, 29
AspS9I GGNCC 1 cut(s) 306
AsuHPI GGTGA 1 cut(s) 29
AsuNHI GCTAGC 1 cut(s) 88
AvaI CYCGRG 1 cut(s) 5
AvaII GGWCC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 9
BccI CCATC 1 cut(s) 230
BcgI CGANNNNNNTGC 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 83
BisI GCNGC 2 cut(s) 23, 258
BlsI GCNGC 2 cut(s) 24, 259
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 5
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 1 cut(s) 307
BmsI GCATC 3 cut(s) 4, 340, 349
BmtI GCTAGC 1 cut(s) 92
BpmI CTGGAG 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 282
BsePI GCGCGC 1 cut(s) 25
BseXI GCAGC 1 cut(s) 9
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 5
BslFI GGGAC 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 1 cut(s) 281
BsoBI CYCGRG 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 291
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 3 cut(s) 257, 260, 303
BspANI GGCC 1 cut(s) 281
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 83
BspOI GCTAGC 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 282
BssHII GCGCGC 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 336
BstAPI GCANNNNNTGC 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 27, 90
BstDSI CCRYGG 1 cut(s) 282
BstFNI CGCG 1 cut(s) 27
BstHHI GCGC 2 cut(s) 27, 29
BstMAI GTCTC 1 cut(s) 257
BstMWI GCNNNNNNNGC 2 cut(s) 106, 359
BstNSI RCATGY 1 cut(s) 71
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 9
BsuRI GGCC 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 282
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 249
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 27, 90
CfoI GCGC 2 cut(s) 27, 29
Cfr13I GGNCC 1 cut(s) 306
CviAII CATG 3 cut(s) 41, 68, 283
CviJI RGCY 6 cut(s) 48, 158, 179, 232, 281, 289
CviKI_1 RGCY 6 cut(s) 48, 158, 179, 232, 281, 289
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 306
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 5
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 306
EcoRI GAATTC 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 3 cut(s) 44, 71, 286
FaiI YATR 6 cut(s) 42, 69, 201, 221, 284, 345
FaqI GGGAC 1 cut(s) 319
FatI CATG 3 cut(s) 40, 67, 282
FauI CCCGC 1 cut(s) 296
Fnu4HI GCNGC 2 cut(s) 23, 258
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 2 cut(s) 23, 258
FspBI CTAG 1 cut(s) 89
GlaI GCGC 2 cut(s) 26, 28
GluI GCNGC 2 cut(s) 23, 258
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 1 cut(s) 281
HhaI GCGC 2 cut(s) 27, 29
Hin1II CATG 3 cut(s) 44, 71, 286
Hin6I GCGC 2 cut(s) 25, 27
HinP1I GCGC 2 cut(s) 25, 27
HinfI GANTC 1 cut(s) 265
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 2 cut(s) 166, 358
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 1 cut(s) 365
HpyCH4III ACNGT 1 cut(s) 336
HpyCH4V TGCA 3 cut(s) 100, 109, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 359
Hsp92II CATG 3 cut(s) 44, 71, 286
HspAI GCGC 2 cut(s) 25, 27
LmnI GCTCC 2 cut(s) 239, 286
LpnPI CCDG 3 cut(s) 78, 179, 254
Lsp1109I GCAGC 1 cut(s) 9
LweI GCATC 3 cut(s) 4, 340, 349
MaeI CTAG 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 52
MboII GAAGA 3 cut(s) 23, 359, 380
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 4 cut(s) 120, 168, 376, 387
MlyI GAGTC 1 cut(s) 259
MnlI CCTC 1 cut(s) 308
MseI TTAA 1 cut(s) 394
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 106, 359
NcoI CCATGG 1 cut(s) 282
NheI GCTAGC 1 cut(s) 88
NlaIII CATG 3 cut(s) 44, 71, 286
NlaIV GGNNCC 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 52
NspI RCATGY 1 cut(s) 71
PaeR7I CTCGAG 1 cut(s) 5
PauI GCGCGC 1 cut(s) 25
PkrI GCNGC 2 cut(s) 24, 259
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 306
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 306
PspPPI RGGWCCY 1 cut(s) 306
PspXI VCTCGAGB 1 cut(s) 5
PteI GCGCGC 1 cut(s) 25
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 1 cut(s) 394
SatI GCNGC 2 cut(s) 23, 258
Sau96I GGNCC 1 cut(s) 306
SchI GAGTC 1 cut(s) 259
SduI GDGCHC 1 cut(s) 291
SetI ASST 8 cut(s) 50, 79, 85, 97, 210, 291, 311, 319
SfaNI GCATC 3 cut(s) 4, 340, 349
Sfr274I CTCGAG 1 cut(s) 5
SinI GGWCC 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 5
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
Sse9I AATT 4 cut(s) 120, 168, 376, 387
SsiI CCGC 3 cut(s) 257, 260, 303
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 291
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 336
TaqI TCGA 4 cut(s) 6, 234, 291, 385
TasI AATT 4 cut(s) 120, 168, 376, 387
TauI GCSGC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 394
Tru9I TTAA 1 cut(s) 394
TscAI CASTG 1 cut(s) 249
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 3 cut(s) 328, 360, 380
TspRI CASTG 1 cut(s) 249
VpaK11BI GGWCC 1 cut(s) 306
XapI RAATTY 2 cut(s) 168, 387
XceI RCATGY 1 cut(s) 71
XhoI CTCGAG 1 cut(s) 5
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.