Rh6BG084600

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0019513
Physical Location & Seq
Forward (+)
13183122 .. 13186114
2993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG084600.1

Sequence Viewer

Length: 294 bp
ATGGAAGGAGCAGTGCTTCGGTTTGCGGCGGAGACTCTGGTGGACAAGGCCATGGAGCTCGACCACATCGGCGGGACCTTTGGAGGAGGATTAGAAAAGGGGCTGCTCCAGCTAGAAGTGTTCTGGCGTGGATCCCAATATAAATTTGAAAGTATGCTTAGGCAGCAACTGTGGTTGATAGCCCCCACAGCTTATATTGACTCACTTCTCAAGAACATGAGTTTCATCAAAGGCGATGAACCACCAGTTTCTCCATCTCGTACTCTGTGTAACCATACTATTAGCAAAACATAA

Protein Analysis

97

Amino Acids

10.83

Weight (kDa)

6.07

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019513)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004135.1_g000003
rosa_rugosa Rorug03G0316400
rosa_samantha Rh2BG035600 Rh5BG425300 Rh5CG449400 Rh6AG240200 Rh6BG084600 Rh7AG481100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 26, 29, 72
AclWI GGATC 2 cut(s) 126, 139
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 1 cut(s) 262
AgsI TTSAA 1 cut(s) 149
AluBI AGCT 3 cut(s) 58, 112, 191
AluI AGCT 3 cut(s) 58, 112, 191
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 2 cut(s) 126, 139
AlwNI CAGNNNCTG 1 cut(s) 169
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 2 cut(s) 103, 163
ApoI RAATTY 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 75
AvaII GGWCC 1 cut(s) 75
BamHI GGATCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 90, 175
BccI CCATC 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 1 cut(s) 113
BisI GCNGC 3 cut(s) 27, 104, 164
BlsI GCNGC 3 cut(s) 28, 105, 165
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 2 cut(s) 76, 133
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 245
BseRI GAGGAG 1 cut(s) 99
BseXI GCAGC 2 cut(s) 90, 175
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 60
BslFI GGGAC 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 1 cut(s) 131
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 3 cut(s) 26, 29, 72
BspANI GGCC 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 76, 133
BspPI GGATC 2 cut(s) 126, 139
BsrI ACTGG 1 cut(s) 245
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 158
BstDSI CCRYGG 1 cut(s) 51
BstKTI GATC 1 cut(s) 134
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 131
BstMWI GCNNNNNNNGC 3 cut(s) 109, 163, 188
BstV1I GCAGC 2 cut(s) 90, 175
BstX2I RGATCY 1 cut(s) 131
BstYI RGATCY 1 cut(s) 131
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
CaiI CAGNNNCTG 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 75
Csp6I GTAC 1 cut(s) 261
CviAII CATG 2 cut(s) 52, 217
CviJI RGCY 6 cut(s) 50, 58, 103, 112, 182, 191
CviKI_1 RGCY 6 cut(s) 50, 58, 103, 112, 182, 191
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 1 cut(s) 158
DpnI GATC 1 cut(s) 133
DpnII GATC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 55, 220
FaiI YATR 7 cut(s) 53, 141, 155, 195, 218, 276, 292
FaqI GGGAC 1 cut(s) 88
FatI CATG 2 cut(s) 51, 216
FauI CCCGC 1 cut(s) 65
Fnu4HI GCNGC 3 cut(s) 27, 104, 164
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 3 cut(s) 27, 104, 164
FspBI CTAG 1 cut(s) 113
GluI GCNGC 3 cut(s) 27, 104, 164
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 1 cut(s) 50
Hin1II CATG 2 cut(s) 55, 220
HinfI GANTC 2 cut(s) 34, 200
Hpy166II GTNNAC 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 163, 188
HpyF3I CTNAG 1 cut(s) 158
Hsp92II CATG 2 cut(s) 55, 220
Kzo9I GATC 1 cut(s) 131
LmnI GCTCC 3 cut(s) 8, 55, 111
LpnPI CCDG 4 cut(s) 23, 109, 122, 258
Lsp1109I GCAGC 2 cut(s) 90, 175
MaeI CTAG 1 cut(s) 113
MaeIII GTNAC 1 cut(s) 269
MalI GATC 1 cut(s) 133
MboI GATC 1 cut(s) 131
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 1 cut(s) 143
MlyI GAGTC 2 cut(s) 28, 194
MnlI CCTC 2 cut(s) 77, 80
MwoI GCNNNNNNNGC 3 cut(s) 109, 163, 188
NcoI CCATGG 1 cut(s) 51
NdeII GATC 1 cut(s) 131
NlaIII CATG 2 cut(s) 55, 220
NlaIV GGNNCC 2 cut(s) 76, 133
PkrI GCNGC 3 cut(s) 28, 105, 165
PleI GAGTC 2 cut(s) 28, 194
PpsI GAGTC 2 cut(s) 28, 194
PpuMI RGGWCCY 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 76, 133
PspPI GGNCC 1 cut(s) 75
PspPPI RGGWCCY 1 cut(s) 75
PstNI CAGNNNCTG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 131
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
SacI GAGCTC 1 cut(s) 60
SatI GCNGC 3 cut(s) 27, 104, 164
Sau3AI GATC 1 cut(s) 131
Sau96I GGNCC 1 cut(s) 75
SchI GAGTC 2 cut(s) 28, 194
SduI GDGCHC 1 cut(s) 60
SetI ASST 4 cut(s) 60, 80, 114, 193
SinI GGWCC 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 143
SsiI CCGC 3 cut(s) 26, 29, 72
SspMI CTAG 1 cut(s) 113
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 171
TaqI TCGA 1 cut(s) 60
TasI AATT 1 cut(s) 143
TauI GCSGC 1 cut(s) 29
TscAI CASTG 1 cut(s) 18
TseI GCWGC 2 cut(s) 103, 163
TspDTI ATGAA 2 cut(s) 214, 252
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 1 cut(s) 143
XspI CTAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.