Rmu_sc0004521.1_g000005

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004521.1
OG0017618
Physical Location & Seq
Forward (+)
22341 .. 23435
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1095 bp
atgtctctcaaaccagttcaagaagctgaaccagcaatttctcagcccagaaattttaagtggtggctccgtatcggcgtctacacactctttgttctctttggccagtcagcagctatactcttgggacgactgtactacaacaaaggtggaaaaagcatatggatggcaaccctagtgcaatttgtagggtttccaatcctaattccttaccaatgtctctcatcatccaaaaaaatcactacagaaagcaatgtgaacctgccatcttcatcagttcttgcattgatctatctctcttttggtgtgctactagcatcagatagcatgctgtattccattggactcttgtaccttccagtttcgacattttcactcatttgtgcatctcagttggtcttcaatgctttcttctccttcttcctcaattcacaaaagttcacaccttacattgtcaattccctagtccttctcaccatctcctccatgttacttgtgtttcatgccaactccataaatgtcgctggagtttctaaagagaagtacataatcggaatcacgtgcaccattggtgcatctgctggctatggtttgatgttttcccttacgcaggtagctttccagaaagttctaaaatcaaatgcatttagtgctgtgttagaaatgataatctttgaatctatagttgccacctgtgctaccctagttggacttttcgtcagcaaagagatgaagggtttaaagagagagattgaggagtttgagctggggaggctattctacttggtgactttggtttgcacagctgcagcatggcaagctttcaatattggtgcaataggactggtttttgaggtttcttccttattctcgaatgttatatgtgtttcgggcttgccatttgttccagttctcgcagtgatattcttcaatgatagtctggatggaataaaggttattgcaacagtgttggcaatctggggatttgcttcatatctctatcagcattatctcgacaattcgaactccaaccctgaaaccaaacctgtaacagaaatttcaatgattcctttactgaaagagaccaactgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

364

Amino Acids

40.0

Weight (kDa)

8.37

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 24 - 341 8.6e-114 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g06160
malus_domestica MD05G1289900.v1.1
prunus_persica Prupe.4G075800_v2.0.a1
rosa_laevigata RLG00000031994
rosa_multiflora Rmu_sc0004521.1_g000005
rosa_roxburghii Rroxscaffold_1G00063410
rosa_rugosa Rorug05G0007800
rosa_samantha Rh5AG102100 Rh5BG098400 Rh5CG110600 Rh5DG097700
rosa_wichuraiana Rw5G008910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 270, 601
AccI GTMKAC 1 cut(s) 81
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 52, 1056
AcvI CACGTG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 3 cut(s) 137, 353, 545
AfiI CCNNNNNNNGG 1 cut(s) 610
AgsI TTSAA 6 cut(s) 20, 403, 677, 826, 931, 1062
AhdI GACNNNNNGTC 1 cut(s) 716
AluBI AGCT 6 cut(s) 26, 116, 617, 766, 806, 821
AluI AGCT 6 cut(s) 26, 116, 617, 766, 806, 821
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 3 cut(s) 9, 224, 1076
Alw44I GTGCAC 1 cut(s) 562
AoxI GGCC 1 cut(s) 103
ApaLI GTGCAC 1 cut(s) 562
ApeKI GCWGC 3 cut(s) 113, 806, 809
ApoI RAATTY 2 cut(s) 52, 1056
AsuHPI GGTGA 2 cut(s) 466, 799
AsuII TTCGAA 1 cut(s) 1022
BaeGI GKGCMC 1 cut(s) 566
BaeI ACNNNNGTAYC 2 cut(s) 335, 368
BalI TGGCCA 1 cut(s) 105
BbrPI CACGTG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 566
BbvI GCAGC 3 cut(s) 125, 793, 821
BccI CCATC 4 cut(s) 160, 274, 485, 938
BcoDI GTCTC 3 cut(s) 9, 224, 1076
BfaI CTAG 4 cut(s) 176, 314, 464, 704
BfmI CTRYAG 3 cut(s) 243, 681, 807
BfuAI ACCTGC 2 cut(s) 270, 601
BisI GCNGC 3 cut(s) 114, 807, 810
BlsI GCNGC 3 cut(s) 115, 808, 811
BmeRI GACNNNNNGTC 1 cut(s) 716
BmiI GGNNCC 1 cut(s) 68
BmsI GCATC 3 cut(s) 326, 395, 584
BpiI GAAGAC 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 1022
BsaAI YACGTR 1 cut(s) 561
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 1076
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse1I ACTGG 5 cut(s) 14, 106, 359, 849, 908
Bse3DI GCAATG 1 cut(s) 259
BseGI GGATG 3 cut(s) 171, 227, 949
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 2 cut(s) 56, 404
BseNI ACTGG 5 cut(s) 14, 106, 359, 849, 908
BseRI GAGGAG 2 cut(s) 472, 770
BseSI GKGCMC 1 cut(s) 566
BseXI GCAGC 3 cut(s) 125, 793, 821
BseYI CCCAGC 1 cut(s) 766
BshFI GGCC 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 566
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 3 cut(s) 9, 224, 1076
BsmFI GGGAC 1 cut(s) 141
BsnI GGCC 1 cut(s) 105
Bso31I GGTCTC 1 cut(s) 1076
Bsp119I TTCGAA 1 cut(s) 1022
Bsp1286I GDGCHC 1 cut(s) 566
Bsp143I GATC 1 cut(s) 288
BspANI GGCC 1 cut(s) 105
BspCNI CTCAG 2 cut(s) 55, 403
BspLI GGNNCC 1 cut(s) 68
BspMAI CTGCAG 1 cut(s) 811
BspMI ACCTGC 2 cut(s) 270, 601
BspT104I TTCGAA 1 cut(s) 1022
BspTNI GGTCTC 1 cut(s) 1076
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 5 cut(s) 14, 106, 359, 849, 908
BssMI GATC 1 cut(s) 288
BssNI GRCGYC 1 cut(s) 78
Bst4CI ACNGT 2 cut(s) 135, 967
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 1 cut(s) 650
BstBAI YACGTR 1 cut(s) 561
BstBI TTCGAA 1 cut(s) 1022
BstC8I GCNNGC 4 cut(s) 329, 583, 819, 896
BstDEI CTNAG 2 cut(s) 42, 390
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 3 cut(s) 171, 227, 949
BstKTI GATC 1 cut(s) 291
BstMAI GTCTC 3 cut(s) 9, 224, 1076
BstMBI GATC 1 cut(s) 288
BstMWI GCNNNNNNNGC 5 cut(s) 32, 650, 695, 772, 818
BstNSI RCATGY 1 cut(s) 331
BstSFI CTRYAG 3 cut(s) 243, 681, 807
BstSLI GKGCMC 1 cut(s) 566
BstV1I GCAGC 3 cut(s) 125, 793, 821
BstV2I GAAGAC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 3 cut(s) 171, 227, 949
BtsI GCAGTG 1 cut(s) 924
BtsIMutI CAGTG 2 cut(s) 924, 972
BveI ACCTGC 2 cut(s) 270, 601
Cac8I GCNNGC 4 cut(s) 329, 583, 819, 896
CseI GACGC 1 cut(s) 67
Csp6I GTAC 3 cut(s) 136, 352, 544
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 4 cut(s) 328, 487, 503, 813
CviQI GTAC 3 cut(s) 136, 352, 544
DdeI CTNAG 2 cut(s) 42, 390
DpnI GATC 1 cut(s) 290
DpnII GATC 1 cut(s) 288
DraI TTTAAA 1 cut(s) 741
DriI GACNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 1 cut(s) 103
Eam1105I GACNNNNNGTC 1 cut(s) 716
Eco31I GGTCTC 1 cut(s) 1076
Eco72I CACGTG 1 cut(s) 561
EcoNI CCTNNNNNAGG 1 cut(s) 608
EcoT22I ATGCAT 1 cut(s) 646
FaeI CATG 4 cut(s) 331, 490, 506, 816
FaqI GGGAC 1 cut(s) 141
FatI CATG 4 cut(s) 327, 486, 502, 812
FauNDI CATATG 1 cut(s) 161
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 3 cut(s) 114, 807, 810
FokI GGATG 3 cut(s) 178, 214, 956
Fsp4HI GCNGC 3 cut(s) 114, 807, 810
FspBI CTAG 4 cut(s) 176, 314, 464, 704
GluI GCNGC 3 cut(s) 114, 807, 810
GsaI CCCAGC 1 cut(s) 770
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 1 cut(s) 105
HgaI GACGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 4 cut(s) 331, 490, 506, 816
HindIII AAGCTT 1 cut(s) 819
HinfI GANTC 4 cut(s) 345, 555, 677, 1066
HphI GGTGA 2 cut(s) 466, 799
Hpy166II GTNNAC 4 cut(s) 82, 259, 441, 564
Hpy188I TCNGA 2 cut(s) 322, 554
Hpy188III TCNNGA 5 cut(s) 20, 622, 871, 941, 1013
Hpy8I GTNNAC 4 cut(s) 82, 259, 441, 564
HpyAV CCTTC 4 cut(s) 365, 427, 479, 727
HpyCH4III ACNGT 2 cut(s) 135, 967
HpyCH4IV ACGT 1 cut(s) 560
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 650, 695, 772, 818
HpyF3I CTNAG 2 cut(s) 42, 390
HpySE526I ACGT 1 cut(s) 560
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 4 cut(s) 331, 490, 506, 816
Kzo9I GATC 1 cut(s) 288
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 3 cut(s) 125, 793, 821
LweI GCATC 3 cut(s) 326, 395, 584
MaeI CTAG 4 cut(s) 176, 314, 464, 704
MaeII ACGT 1 cut(s) 560
MaeIII GTNAC 3 cut(s) 489, 787, 1048
MalI GATC 1 cut(s) 290
MboI GATC 1 cut(s) 288
MboII GAAGA 6 cut(s) 261, 391, 403, 412, 852, 919
MhlI GDGCHC 1 cut(s) 566
MlsI TGGCCA 1 cut(s) 105
MluCI AATT 8 cut(s) 36, 52, 182, 204, 427, 457, 1018, 1056
MluNI TGGCCA 1 cut(s) 105
MlyI GAGTC 1 cut(s) 339
MmeI TCCRAC 2 cut(s) 688, 1053
MnlI CCTC 5 cut(s) 434, 493, 748, 765, 847
Mox20I TGGCCA 1 cut(s) 105
Mph1103I ATGCAT 1 cut(s) 646
MscI TGGCCA 1 cut(s) 105
MseI TTAA 2 cut(s) 57, 740
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 806
MwoI GCNNNNNNNGC 5 cut(s) 32, 650, 695, 772, 818
NdeI CATATG 1 cut(s) 161
NdeII GATC 1 cut(s) 288
NlaIII CATG 4 cut(s) 331, 490, 506, 816
NlaIV GGNNCC 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 787
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 1 cut(s) 331
NspV TTCGAA 1 cut(s) 1022
PaeI GCATGC 1 cut(s) 331
PfeI GAWTC 3 cut(s) 555, 677, 1066
PkrI GCNGC 3 cut(s) 115, 808, 811
PleI GAGTC 1 cut(s) 339
PmaCI CACGTG 1 cut(s) 561
PmlI CACGTG 1 cut(s) 561
PpsI GAGTC 1 cut(s) 339
Ppu21I YACGTR 1 cut(s) 561
PspCI CACGTG 1 cut(s) 561
PspFI CCCAGC 1 cut(s) 766
PspN4I GGNNCC 1 cut(s) 68
PstI CTGCAG 1 cut(s) 811
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 3 cut(s) 137, 353, 545
RsaNI GTAC 3 cut(s) 136, 352, 544
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 2 cut(s) 57, 740
SatI GCNGC 3 cut(s) 114, 807, 810
Sau3AI GATC 1 cut(s) 288
SchI GAGTC 1 cut(s) 339
SduI GDGCHC 1 cut(s) 566
SfaNI GCATC 3 cut(s) 326, 395, 584
SfcI CTRYAG 3 cut(s) 243, 681, 807
SfuI TTCGAA 1 cut(s) 1022
SmiMI CAYNNNNRTG 1 cut(s) 164
SphI GCATGC 1 cut(s) 331
Sse9I AATT 8 cut(s) 36, 52, 182, 204, 427, 457, 1018, 1056
SspI AATATT 1 cut(s) 829
SspMI CTAG 4 cut(s) 176, 314, 464, 704
TaaI ACNGT 2 cut(s) 135, 967
TaiI ACGT 1 cut(s) 563
TaqI TCGA 4 cut(s) 365, 872, 1014, 1022
TasI AATT 8 cut(s) 36, 52, 182, 204, 427, 457, 1018, 1056
TatI WGTACW 2 cut(s) 135, 543
TfiI GAWTC 3 cut(s) 555, 677, 1066
Tru1I TTAA 2 cut(s) 57, 740
Tru9I TTAA 2 cut(s) 57, 740
TscAI CASTG 2 cut(s) 924, 972
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 3 cut(s) 113, 806, 809
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 4 cut(s) 261, 491, 746, 981
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 2 cut(s) 924, 972
VneI GTGCAC 1 cut(s) 562
XagI CCTNNNNNAGG 1 cut(s) 608
XapI RAATTY 2 cut(s) 52, 1056
XceI RCATGY 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 4 cut(s) 176, 314, 464, 704
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.