Rmu_sc0004574.1_g000055

At5g08430

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004574.1
OG0019093
Physical Location & Seq
Reverse (-)
174477 .. 175034
558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ctggagataccggcggggcagaacacttggcggtccggctacagtggccgtggtagggatgggccaggcttggctgacgaagcagattcccaatacaatgtcgctacgattatcaacgactacgtcaaccaccgtaggcttctatacccgacgaagaagaagaggatcgtctgtgaccaagggattcattctctattcggcaggaaaaccattgcttgtatcaagatctacaatttattccggccgcatttcactgataatctcgaggatgattccgatgattcgtcggactcatccgacgacaacggtttggataaggaggacgaggataagtggcggcagcagcagaggaagaagttaaagaggagagccgccgctatgcatacgtctcctcctgttccgaaaagctgctacgccgccgtgattcgggagaatttgaagctggtgtaccttaggaggtcggtggtggaagctctgctgaagcaggatgaggcacaggtgtttgaggacaaactcgtagggagctttgtgaggacgaaatcttcttcagcttcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

185

Amino Acids

21.02

Weight (kDa)

8.92

Isoelectric Point (pI)

54.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 27 - 84 1.1e-06 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019093)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004574.1_g000055
rosa_samantha Rh2AG152600 Rh2BG103400 Rh2CG105800 Rh3BG058600 Rh3BG256700 Rh3CG057800 Rh3DG059100 Rh4CG015600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 14, 31, 245, 337, 372, 375, 417
AclWI GGATC 1 cut(s) 173
AcoI YGGCCR 2 cut(s) 46, 242
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 2 cut(s) 500, 531
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 2 cut(s) 55, 426
AgsI TTSAA 1 cut(s) 439
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 5 cut(s) 408, 442, 473, 525, 551
AluI AGCT 5 cut(s) 408, 442, 473, 525, 551
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 3 cut(s) 46, 62, 242
ApeKI GCWGC 3 cut(s) 340, 343, 408
ApoI RAATTY 1 cut(s) 433
AspS9I GGNCC 2 cut(s) 33, 62
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 33
AxyI CCTNAGG 1 cut(s) 452
BarI GAAGNNNNNNTAC 2 cut(s) 431, 463
BbvI GCAGC 3 cut(s) 352, 355, 395
BccI CCATC 1 cut(s) 53
BceAI ACGGC 2 cut(s) 33, 404
BciT130I CCWGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 40
BglII AGATCT 1 cut(s) 225
BisI GCNGC 8 cut(s) 245, 338, 341, 344, 372, 375, 409, 417
BlsI GCNGC 8 cut(s) 246, 339, 342, 345, 373, 376, 410, 418
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 1 cut(s) 33
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 33, 62
BmrFI CCNGG 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 49, 178
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 426
Bse118I RCCGGY 1 cut(s) 10
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 49, 178
BseGI GGATG 4 cut(s) 64, 274, 293, 493
BseLI CCNNNNNNNGG 2 cut(s) 55, 426
BseMI GCAATG 1 cut(s) 210
BseRI GAGGAG 2 cut(s) 379, 381
BseX3I CGGCCG 1 cut(s) 242
BseXI GCAGC 3 cut(s) 352, 355, 395
Bsh1285I CGRYCG 1 cut(s) 245
BshFI GGCC 3 cut(s) 48, 64, 244
BsiEI CGRYCG 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 3 cut(s) 11, 36, 241
BslI CCNNNNNNNGG 2 cut(s) 55, 426
BsmAI GTCTC 1 cut(s) 393
BsmBI CGTCTC 1 cut(s) 393
BsnI GGCC 3 cut(s) 48, 64, 244
BsoBI CYCGRG 1 cut(s) 263
Bsp143I GATC 2 cut(s) 165, 225
BspACI CCGC 7 cut(s) 14, 31, 245, 337, 372, 375, 417
BspANI GGCC 3 cut(s) 48, 64, 244
BspHI TCATGA 1 cut(s) 554
BspPI GGATC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 49, 178
BssMI GATC 2 cut(s) 165, 225
BssT1I CCWWGG 1 cut(s) 178
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 3 cut(s) 44, 134, 308
Bst6I CTCTTC 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 452
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 4 cut(s) 64, 274, 293, 493
BstKTI GATC 2 cut(s) 168, 228
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 2 cut(s) 165, 225
BstMCI CGRYCG 1 cut(s) 245
BstMWI GCNNNNNNNGC 3 cut(s) 45, 80, 343
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 1 cut(s) 40
BstV1I GCAGC 3 cut(s) 352, 355, 395
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BstZI CGGCCG 1 cut(s) 242
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 3 cut(s) 48, 64, 244
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 4 cut(s) 64, 274, 293, 493
BtsIMutI CAGTG 2 cut(s) 49, 252
CciI TCATGA 1 cut(s) 554
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 33, 62
CpoI CGGWCCG 1 cut(s) 33
Csp6I GTAC 1 cut(s) 448
CspI CGGWCCG 1 cut(s) 33
CviAII CATG 1 cut(s) 555
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 1 cut(s) 452
DpnI GATC 2 cut(s) 167, 227
DpnII GATC 2 cut(s) 165, 225
EaeI YGGCCR 2 cut(s) 46, 242
EagI CGGCCG 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
EclXI CGGCCG 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 33
Eco52I CGGCCG 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 500, 531
Eco81I CCTNAGG 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 178
EcoT22I ATGCAT 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 1 cut(s) 558
FaiI YATR 4 cut(s) 145, 380, 384, 556
FatI CATG 1 cut(s) 554
FauI CCCGC 1 cut(s) 7
Fnu4HI GCNGC 8 cut(s) 245, 338, 341, 344, 372, 375, 409, 417
FokI GGATG 4 cut(s) 71, 280, 281, 500
Fsp4HI GCNGC 8 cut(s) 245, 338, 341, 344, 372, 375, 409, 417
GluI GCNGC 8 cut(s) 245, 338, 341, 344, 372, 375, 409, 417
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 3 cut(s) 48, 64, 244
HapII CCGG 3 cut(s) 11, 36, 241
Hin1II CATG 1 cut(s) 558
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 6 cut(s) 86, 184, 272, 281, 290, 424
HpaII CCGG 3 cut(s) 11, 36, 241
Hpy166II GTNNAC 2 cut(s) 127, 448
Hpy188I TCNGA 4 cut(s) 277, 289, 298, 402
Hpy188III TCNNGA 4 cut(s) 223, 263, 428, 555
Hpy8I GTNNAC 2 cut(s) 127, 448
Hpy99I CGWCG 3 cut(s) 154, 289, 302
HpyCH4III ACNGT 3 cut(s) 44, 134, 308
HpyCH4IV ACGT 2 cut(s) 123, 386
HpyCH4V TGCA 1 cut(s) 382
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 80, 343
HpyF3I CTNAG 1 cut(s) 452
HpySE526I ACGT 2 cut(s) 123, 386
Hsp92II CATG 1 cut(s) 558
Kzo9I GATC 2 cut(s) 165, 225
LmnI GCTCC 1 cut(s) 522
Lsp1109I GCAGC 3 cut(s) 352, 355, 395
MaeII ACGT 2 cut(s) 123, 386
MaeIII GTNAC 1 cut(s) 173
MalI GATC 2 cut(s) 167, 227
MboI GATC 2 cut(s) 165, 225
MboII GAAGA 6 cut(s) 166, 169, 172, 364, 534, 537
MflI RGATCY 1 cut(s) 225
MluCI AATT 2 cut(s) 232, 433
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 2 cut(s) 267, 321
Mph1103I ATGCAT 1 cut(s) 384
MseI TTAA 1 cut(s) 359
MspI CCGG 3 cut(s) 11, 36, 241
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 45, 80, 343
NdeII GATC 2 cut(s) 165, 225
NlaIII CATG 1 cut(s) 558
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 1 cut(s) 384
PaeR7I CTCGAG 1 cut(s) 263
PagI TCATGA 1 cut(s) 554
PfeI GAWTC 5 cut(s) 86, 184, 272, 281, 424
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 8 cut(s) 246, 339, 342, 345, 373, 376, 410, 418
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspPI GGNCC 2 cut(s) 33, 62
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 122
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
Rsr2I CGGWCCG 1 cut(s) 33
RsrII CGGWCCG 1 cut(s) 33
SaqAI TTAA 1 cut(s) 359
SatI GCNGC 8 cut(s) 245, 338, 341, 344, 372, 375, 409, 417
Sau3AI GATC 2 cut(s) 165, 225
Sau96I GGNCC 2 cut(s) 33, 62
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 66
SfcI CTRYAG 1 cut(s) 40
Sfr274I CTCGAG 1 cut(s) 263
SinI GGWCC 1 cut(s) 33
SlaI CTCGAG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 2 cut(s) 232, 433
SsiI CCGC 7 cut(s) 14, 31, 245, 337, 372, 375, 417
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 3 cut(s) 44, 134, 308
TaiI ACGT 2 cut(s) 126, 389
TaqI TCGA 1 cut(s) 264
TasI AATT 2 cut(s) 232, 433
TauI GCSGC 5 cut(s) 247, 340, 374, 377, 419
TfiI GAWTC 5 cut(s) 86, 184, 272, 281, 424
Tru1I TTAA 1 cut(s) 359
Tru9I TTAA 1 cut(s) 359
TscAI CASTG 2 cut(s) 49, 259
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 3 cut(s) 340, 343, 408
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 176, 543
TspRI CASTG 2 cut(s) 49, 259
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 1 cut(s) 433
XhoI CTCGAG 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.