Rh2CG105800

At5g08430

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0019093
Physical Location & Seq
Forward (+)
9134688 .. 9135164
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG105800.1

Sequence Viewer

Length: 477 bp
ATGGGCCAGGCTTGGCTGACGAAGCAGATCTCCCAATACAATGTCGCTACGATTATCACCGACTACGTCAACCACCGTAGGCTTCTATACCCGACGAAGAAGAAGAGGATCGTCTGTGACCAAGGGATTCATTCTCTATTCGGCAGGAAAACCATTGCTTGGATCAAGATCTACAATTTATTCCGGCCGCATTTCACTGATAATCTCGAGGATGATTCCGATGATTTGTCGGATGAGGAGGACGACGATAAGTGGCTGCAGCAGCAGAGGAAGAAGCTGAAGAGGAGAGCCGCCGCTCTGCATACGCCTCCTCCTGTTTCGAAAAGTTGCTACGCCGCCGTGATTCCGGAGAATTTGAAGCTGGTGTACCTTAGGAGGTTGGTGGTGGAAGCTCTGCTGAAGCAGGACGAGGCTCAGGTGTTTGAGGACAAACTCGTGGGGAGCTTTGTGAGGACGAAATCTTCTTCAGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

158

Amino Acids

18.33

Weight (kDa)

9.32

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 7 - 65 5.1e-07 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019093)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004574.1_g000055
rosa_samantha Rh2AG152600 Rh2BG103400 Rh2CG105800 Rh3BG058600 Rh3BG256700 Rh3CG057800 Rh3DG059100 Rh4CG015600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 159
AccBSI CCGCTC 1 cut(s) 296
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 4 cut(s) 188, 291, 294, 336
AclWI GGATC 2 cut(s) 116, 170
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 1 cut(s) 352
AcuI CTGAAG 3 cut(s) 299, 419, 450
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 1 cut(s) 358
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 5 cut(s) 277, 361, 392, 444, 470
AluI AGCT 5 cut(s) 277, 361, 392, 444, 470
AlwI GGATC 2 cut(s) 116, 170
Ama87I CYCGRG 1 cut(s) 206
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 2 cut(s) 4, 185
ApeKI GCWGC 3 cut(s) 256, 259, 262
ApoI RAATTY 1 cut(s) 352
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 49
AsuII TTCGAA 1 cut(s) 320
AvaI CYCGRG 1 cut(s) 206
AxyI CCTNAGG 1 cut(s) 371
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
BauI CACGAG 1 cut(s) 434
BbvI GCAGC 3 cut(s) 243, 271, 274
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 257
BglII AGATCT 2 cut(s) 27, 168
BisI GCNGC 7 cut(s) 188, 257, 260, 263, 291, 294, 336
BlsI GCNGC 7 cut(s) 189, 258, 261, 264, 292, 295, 337
Bme1390I CCNGG 1 cut(s) 8
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 121
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse21I CCTNAGG 1 cut(s) 371
Bse3DI GCAATG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 167
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 121
BseGI GGATG 2 cut(s) 217, 238
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 428
BseRI GAGGAG 3 cut(s) 251, 298, 300
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 3 cut(s) 243, 271, 274
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 2 cut(s) 6, 187
BsiEI CGRYCG 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 2 cut(s) 184, 347
BslI CCNNNNNNNGG 1 cut(s) 159
BsnI GGCC 2 cut(s) 6, 187
BsoBI CYCGRG 1 cut(s) 206
Bsp119I TTCGAA 1 cut(s) 320
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 4 cut(s) 27, 108, 162, 168
BspACI CCGC 4 cut(s) 188, 291, 294, 336
BspANI GGCC 2 cut(s) 6, 187
BspCNI CTCAG 1 cut(s) 427
BspEI TCCGGA 1 cut(s) 346
BspHI TCATGA 1 cut(s) 473
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 2 cut(s) 116, 170
BspT104I TTCGAA 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 4 cut(s) 27, 108, 162, 168
BssSI CACGAG 1 cut(s) 434
BssT1I CCWWGG 1 cut(s) 121
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 98, 275
BstBI TTCGAA 1 cut(s) 320
BstDEI CTNAG 2 cut(s) 371, 414
BstF5I GGATG 2 cut(s) 217, 238
BstKTI GATC 4 cut(s) 30, 111, 165, 171
BstMBI GATC 4 cut(s) 27, 108, 162, 168
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 2 cut(s) 22, 262
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 6
BstSFI CTRYAG 1 cut(s) 257
BstV1I GCAGC 3 cut(s) 243, 271, 274
BstX2I RGATCY 2 cut(s) 27, 168
BstYI RGATCY 2 cut(s) 27, 168
BstZI CGGCCG 1 cut(s) 185
Bsu36I CCTNAGG 1 cut(s) 371
BsuRI GGCC 2 cut(s) 6, 187
BtsCI GGATG 2 cut(s) 217, 238
BtsIMutI CAGTG 1 cut(s) 195
CciI TCATGA 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 367
CviAII CATG 1 cut(s) 474
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 371, 414
DpnI GATC 4 cut(s) 29, 110, 164, 170
DpnII GATC 4 cut(s) 27, 108, 162, 168
EaeI YGGCCR 1 cut(s) 185
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 2 cut(s) 98, 275
EarI CTCTTC 2 cut(s) 98, 275
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 121
Eco52I CGGCCG 1 cut(s) 185
Eco57I CTGAAG 3 cut(s) 299, 419, 450
Eco81I CCTNAGG 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 1 cut(s) 477
FaiI YATR 3 cut(s) 88, 303, 475
FatI CATG 1 cut(s) 473
Fnu4HI GCNGC 7 cut(s) 188, 257, 260, 263, 291, 294, 336
FokI GGATG 2 cut(s) 224, 245
Fsp4HI GCNGC 7 cut(s) 188, 257, 260, 263, 291, 294, 336
GluI GCNGC 7 cut(s) 188, 257, 260, 263, 291, 294, 336
HaeIII GGCC 2 cut(s) 6, 187
HapII CCGG 2 cut(s) 184, 347
Hin1II CATG 1 cut(s) 477
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 3 cut(s) 127, 215, 343
HpaII CCGG 2 cut(s) 184, 347
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 70, 367
Hpy188I TCNGA 2 cut(s) 220, 232
Hpy188III TCNNGA 4 cut(s) 166, 206, 347, 474
Hpy8I GTNNAC 2 cut(s) 70, 367
Hpy99I CGWCG 2 cut(s) 97, 248
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 2 cut(s) 259, 301
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 262
HpyF3I CTNAG 2 cut(s) 371, 414
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 1 cut(s) 477
Kpn2I TCCGGA 1 cut(s) 346
Kzo9I GATC 4 cut(s) 27, 108, 162, 168
LmnI GCTCC 1 cut(s) 441
LpnPI CCDG 8 cut(s) 20, 130, 197, 327, 347, 360, 389, 401
Lsp1109I GCAGC 3 cut(s) 243, 271, 274
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 116
MalI GATC 4 cut(s) 29, 110, 164, 170
MbiI CCGCTC 1 cut(s) 296
MboI GATC 4 cut(s) 27, 108, 162, 168
MboII GAAGA 7 cut(s) 109, 112, 115, 283, 292, 453, 456
MflI RGATCY 2 cut(s) 27, 168
MluCI AATT 2 cut(s) 175, 352
MmeI TCCRAC 1 cut(s) 210
MroI TCCGGA 1 cut(s) 346
MspI CCGG 2 cut(s) 184, 347
MspR9I CCNGG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 8
MwoI GCNNNNNNNGC 2 cut(s) 22, 262
NdeII GATC 4 cut(s) 27, 108, 162, 168
NlaIII CATG 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 116
NspV TTCGAA 1 cut(s) 320
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 473
PfeI GAWTC 3 cut(s) 127, 215, 343
PflFI GACNNNGTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 159
PkrI GCNGC 7 cut(s) 189, 258, 261, 264, 292, 295, 337
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 2 cut(s) 27, 168
PsyI GACNNNGTC 1 cut(s) 65
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SatI GCNGC 7 cut(s) 188, 257, 260, 263, 291, 294, 336
Sau3AI GATC 4 cut(s) 27, 108, 162, 168
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 8
SetI ASST 9 cut(s) 69, 279, 363, 372, 380, 394, 420, 446, 472
SfcI CTRYAG 1 cut(s) 257
Sfr274I CTCGAG 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 320
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 2 cut(s) 175, 352
SsiI CCGC 4 cut(s) 188, 291, 294, 336
StyD4I CCNGG 1 cut(s) 6
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 1 cut(s) 69
TaqI TCGA 2 cut(s) 207, 320
TasI AATT 2 cut(s) 175, 352
TauI GCSGC 4 cut(s) 190, 293, 296, 338
TfiI GAWTC 3 cut(s) 127, 215, 343
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 3 cut(s) 256, 259, 262
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 2 cut(s) 119, 462
TspRI CASTG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 65
Van91I CCANNNNNTGG 1 cut(s) 159
XapI RAATTY 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.