Rh4CG015600

At5g08430

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0019093
Physical Location & Seq
Forward (+)
2893772 .. 2901316
7545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG015600.1

Sequence Viewer

Length: 789 bp
ATGCCGATGAAATTAGGACGACTCCGACCTGATTCGTATACCGATTCTGACAGGCGCGACTCGTTTCCGGCGAGGAATTCCAGCAAGCGAACTATCAAAAGCAAAGCAAAGCTCTGTCCCAAGCTCTTAACCAATTCTCAGGTGGGGTTCCTTGAATTCGATCTGGGGAGGGTGCTGTTTAGCAGATGTAACGCTTACCCATGGCGCGGCGATGGCGGTCCGGCGGCGGTCTGGAGATACCGGCGGGGCAGAACACTTGGCGGTCCGGCTACAGTGGCCGTGGTGGGGATGGGCCAGGCTTGGCTGACGAAGCAGATCTCCCAATACAATGTCGCTACGATTATCACCGACTACGTCAACCACCGTAGGCTTCTATACCCGACGAAGAAGAAGAGGATCGTCTGTGACCAAGGGATTCATTCTCTATTCGGCAGGAAAACCATTGCTTGGATCAAGATCTACAATTTATTCCGGCCGCATTTCATTGATAATCTCGAGGATGATTCCGATGATTCGTCGGATGAGTCCGACGACAACGTTTTGGATGAGGAGGACGAGGATAAGTGGCTGCAGCAGCAGAGGAAGAAGCTGAAGAGGAGAGTCGCCGCTCTGCATACGCCTCCTCCTGTTCCGAAAAGCTGCTACGCCGCCGTGATTCCGGAGAATTTGAAGCTGGTGTACCTTAGGAGGTCGGTGGTGGAAGCTCTGCTGAAGCAGGACGAGGCTCAGGTGTTTGAGGACAAACTCGTGGGGAGCTTTGTGAGGACGAAATCTTCTTCAGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

262

Amino Acids

29.92

Weight (kDa)

9.67

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 103 - 161 9.7e-07 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019093)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004574.1_g000055
rosa_samantha Rh2AG152600 Rh2BG103400 Rh2CG105800 Rh3BG058600 Rh3BG256700 Rh3CG057800 Rh3DG059100 Rh4CG015600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 447
AccBSI CCGCTC 1 cut(s) 608
AccI GTMKAC 1 cut(s) 38
AccII CGCG 2 cut(s) 57, 207
AccIII TCCGGA 1 cut(s) 658
AciI CCGC 9 cut(s) 207, 216, 224, 227, 244, 261, 476, 606, 648
AclI AACGTT 1 cut(s) 537
AclWI GGATC 2 cut(s) 404, 458
AcoI YGGCCR 2 cut(s) 276, 473
AcsI RAATTY 3 cut(s) 76, 155, 664
AcuI CTGAAG 3 cut(s) 611, 731, 762
AfaI GTAC 1 cut(s) 680
AfiI CCNNNNNNNGG 3 cut(s) 206, 285, 447
AgsI TTSAA 2 cut(s) 155, 670
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 8 cut(s) 112, 124, 589, 639, 673, 704, 756, 782
AluI AGCT 8 cut(s) 112, 124, 589, 639, 673, 704, 756, 782
AlwI GGATC 2 cut(s) 404, 458
Ama87I CYCGRG 1 cut(s) 494
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 3 cut(s) 276, 292, 473
ApeKI GCWGC 4 cut(s) 568, 571, 574, 639
ApoI RAATTY 3 cut(s) 76, 155, 664
AspLEI GCGC 2 cut(s) 57, 207
AspS9I GGNCC 3 cut(s) 218, 263, 292
AsuHPI GGTGA 1 cut(s) 337
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 2 cut(s) 218, 263
AxyI CCTNAGG 1 cut(s) 683
BarI GAAGNNNNNNTAC 2 cut(s) 662, 694
BauI CACGAG 1 cut(s) 746
BbvI GCAGC 4 cut(s) 555, 583, 586, 626
BccI CCATC 2 cut(s) 206, 283
BceAI ACGGC 2 cut(s) 263, 635
BciT130I CCWGG 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 270, 569
BglII AGATCT 2 cut(s) 315, 456
BisI GCNGC 9 cut(s) 208, 225, 476, 569, 572, 575, 606, 640, 648
BlsI GCNGC 9 cut(s) 209, 226, 477, 570, 573, 576, 607, 641, 649
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 218, 263
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 3 cut(s) 218, 263, 292
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 1 cut(s) 296
BpmI CTGGAG 1 cut(s) 253
Bpu10I CCTNAGC 1 cut(s) 726
BsaBI GATNNNNATC 1 cut(s) 455
BsaJI CCNNGG 3 cut(s) 200, 279, 409
BsaWI WCCGGW 1 cut(s) 658
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 285, 447
Bse118I RCCGGY 1 cut(s) 240
Bse21I CCTNAGG 1 cut(s) 683
Bse3DI GCAATG 1 cut(s) 441
Bse8I GATNNNNATC 1 cut(s) 455
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 200, 279, 409
BseGI GGATG 4 cut(s) 294, 505, 526, 550
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 3 cut(s) 206, 285, 447
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 2 cut(s) 152, 740
BseRI GAGGAG 3 cut(s) 563, 610, 612
BseX3I CGGCCG 1 cut(s) 473
BseXI GCAGC 4 cut(s) 555, 583, 586, 626
Bsh1236I CGCG 2 cut(s) 57, 207
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 3 cut(s) 278, 294, 475
BsiEI CGRYCG 1 cut(s) 476
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 6 cut(s) 68, 221, 241, 266, 472, 659
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 3 cut(s) 206, 285, 447
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 3 cut(s) 278, 294, 475
BsoBI CYCGRG 1 cut(s) 494
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 5 cut(s) 160, 315, 396, 450, 456
Bsp19I CCATGG 1 cut(s) 200
BspACI CCGC 9 cut(s) 207, 216, 224, 227, 244, 261, 476, 606, 648
BspANI GGCC 3 cut(s) 278, 294, 475
BspCNI CTCAG 2 cut(s) 151, 739
BspEI TCCGGA 1 cut(s) 658
BspFNI CGCG 2 cut(s) 57, 207
BspHI TCATGA 1 cut(s) 785
BspLI GGNNCC 1 cut(s) 149
BspMAI CTGCAG 1 cut(s) 573
BspPI GGATC 2 cut(s) 404, 458
BsrBI CCGCTC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 3 cut(s) 200, 279, 409
BssMI GATC 5 cut(s) 160, 315, 396, 450, 456
BssNAI GTATAC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 746
BssT1I CCWWGG 2 cut(s) 200, 409
Bst1107I GTATAC 1 cut(s) 39
Bst2BI CACGAG 1 cut(s) 746
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 274, 365
Bst6I CTCTTC 2 cut(s) 386, 587
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 3 cut(s) 138, 683, 726
BstDSI CCRYGG 2 cut(s) 200, 279
BstF5I GGATG 4 cut(s) 294, 505, 526, 550
BstFNI CGCG 2 cut(s) 57, 207
BstHHI GCGC 2 cut(s) 57, 207
BstKTI GATC 5 cut(s) 163, 318, 399, 453, 459
BstMBI GATC 5 cut(s) 160, 315, 396, 450, 456
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 4 cut(s) 213, 275, 310, 574
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 2 cut(s) 270, 569
BstUI CGCG 2 cut(s) 57, 207
BstV1I GCAGC 4 cut(s) 555, 583, 586, 626
BstX2I RGATCY 2 cut(s) 315, 456
BstYI RGATCY 2 cut(s) 315, 456
BstZ17I GTATAC 1 cut(s) 39
BstZI CGGCCG 1 cut(s) 473
Bsu36I CCTNAGG 1 cut(s) 683
BsuRI GGCC 3 cut(s) 278, 294, 475
BtgI CCRYGG 2 cut(s) 200, 279
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 4 cut(s) 294, 505, 526, 550
BtsIMutI CAGTG 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 86
CciI TCATGA 1 cut(s) 785
CfoI GCGC 2 cut(s) 57, 207
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 3 cut(s) 218, 263, 292
CpoI CGGWCCG 2 cut(s) 218, 263
Csp6I GTAC 1 cut(s) 679
CspI CGGWCCG 2 cut(s) 218, 263
CviAII CATG 2 cut(s) 201, 786
CviQI GTAC 1 cut(s) 679
DdeI CTNAG 3 cut(s) 138, 683, 726
DpnI GATC 5 cut(s) 162, 317, 398, 452, 458
DpnII GATC 5 cut(s) 160, 315, 396, 450, 456
EaeI YGGCCR 2 cut(s) 276, 473
EagI CGGCCG 1 cut(s) 473
Eam1104I CTCTTC 2 cut(s) 386, 587
EarI CTCTTC 2 cut(s) 386, 587
EclXI CGGCCG 1 cut(s) 473
Eco130I CCWWGG 2 cut(s) 200, 409
Eco47I GGWCC 2 cut(s) 218, 263
Eco52I CGGCCG 1 cut(s) 473
Eco57I CTGAAG 3 cut(s) 611, 731, 762
Eco81I CCTNAGG 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 494
EcoRI GAATTC 2 cut(s) 76, 155
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 200, 409
ErhI CCWWGG 2 cut(s) 200, 409
FaeI CATG 2 cut(s) 204, 789
FaiI YATR 5 cut(s) 39, 202, 376, 615, 787
FaqI GGGAC 1 cut(s) 102
FatI CATG 2 cut(s) 200, 785
FauI CCCGC 1 cut(s) 237
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 9 cut(s) 208, 225, 476, 569, 572, 575, 606, 640, 648
FokI GGATG 4 cut(s) 301, 512, 533, 557
Fsp4HI GCNGC 9 cut(s) 208, 225, 476, 569, 572, 575, 606, 640, 648
GlaI GCGC 2 cut(s) 56, 206
GluI GCNGC 9 cut(s) 208, 225, 476, 569, 572, 575, 606, 640, 648
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 3 cut(s) 278, 294, 475
HapII CCGG 6 cut(s) 68, 221, 241, 266, 472, 659
HhaI GCGC 2 cut(s) 57, 207
Hin1II CATG 2 cut(s) 204, 789
Hin6I GCGC 2 cut(s) 55, 205
HinP1I GCGC 2 cut(s) 55, 205
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HpaII CCGG 6 cut(s) 68, 221, 241, 266, 472, 659
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 3 cut(s) 39, 358, 679
Hpy188I TCNGA 6 cut(s) 26, 49, 508, 520, 529, 633
Hpy188III TCNNGA 5 cut(s) 232, 454, 494, 659, 786
Hpy8I GTNNAC 3 cut(s) 39, 358, 679
Hpy99I CGWCG 3 cut(s) 385, 520, 533
HpyCH4III ACNGT 2 cut(s) 274, 365
HpyCH4IV ACGT 2 cut(s) 354, 537
HpyCH4V TGCA 2 cut(s) 571, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 213, 275, 310, 574
HpyF3I CTNAG 3 cut(s) 138, 683, 726
HpySE526I ACGT 2 cut(s) 354, 537
Hsp92II CATG 2 cut(s) 204, 789
HspAI GCGC 2 cut(s) 55, 205
Kpn2I TCCGGA 1 cut(s) 658
Kzo9I GATC 5 cut(s) 160, 315, 396, 450, 456
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 4 cut(s) 555, 583, 586, 626
MaeII ACGT 2 cut(s) 354, 537
MaeIII GTNAC 2 cut(s) 188, 404
MalI GATC 5 cut(s) 162, 317, 398, 452, 458
MbiI CCGCTC 1 cut(s) 608
MboI GATC 5 cut(s) 160, 315, 396, 450, 456
MboII GAAGA 7 cut(s) 397, 400, 403, 595, 604, 765, 768
MflI RGATCY 2 cut(s) 315, 456
MluCI AATT 6 cut(s) 11, 76, 133, 155, 463, 664
MlyI GAGTC 4 cut(s) 15, 53, 533, 609
MmeI TCCRAC 3 cut(s) 49, 498, 552
MroI TCCGGA 1 cut(s) 658
MseI TTAA 1 cut(s) 128
MspI CCGG 6 cut(s) 68, 221, 241, 266, 472, 659
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 2 cut(s) 57, 207
MwoI GCNNNNNNNGC 4 cut(s) 213, 275, 310, 574
NcoI CCATGG 1 cut(s) 200
NdeII GATC 5 cut(s) 160, 315, 396, 450, 456
NlaIII CATG 2 cut(s) 204, 789
NlaIV GGNNCC 1 cut(s) 149
NmuCI GTSAC 1 cut(s) 404
PaeR7I CTCGAG 1 cut(s) 494
PagI TCATGA 1 cut(s) 785
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PfeI GAWTC 6 cut(s) 32, 44, 415, 503, 512, 655
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 9 cut(s) 209, 226, 477, 570, 573, 576, 607, 641, 649
PleI GAGTC 4 cut(s) 15, 53, 532, 608
PpsI GAGTC 4 cut(s) 15, 53, 532, 608
Psp1406I AACGTT 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 149
PspPI GGNCC 3 cut(s) 218, 263, 292
PstI CTGCAG 1 cut(s) 573
PsuI RGATCY 2 cut(s) 315, 456
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 1 cut(s) 680
RsaNI GTAC 1 cut(s) 679
Rsr2I CGGWCCG 2 cut(s) 218, 263
RsrII CGGWCCG 2 cut(s) 218, 263
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 9 cut(s) 208, 225, 476, 569, 572, 575, 606, 640, 648
Sau3AI GATC 5 cut(s) 160, 315, 396, 450, 456
Sau96I GGNCC 3 cut(s) 218, 263, 292
SchI GAGTC 4 cut(s) 15, 53, 533, 609
ScrFI CCNGG 1 cut(s) 296
SfcI CTRYAG 2 cut(s) 270, 569
Sfr274I CTCGAG 1 cut(s) 494
SinI GGWCC 2 cut(s) 218, 263
SlaI CTCGAG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 6 cut(s) 11, 76, 133, 155, 463, 664
SsiI CCGC 9 cut(s) 207, 216, 224, 227, 244, 261, 476, 606, 648
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 2 cut(s) 200, 409
TaaI ACNGT 2 cut(s) 274, 365
TaiI ACGT 2 cut(s) 357, 540
TaqI TCGA 2 cut(s) 159, 495
TasI AATT 6 cut(s) 11, 76, 133, 155, 463, 664
TauI GCSGC 5 cut(s) 210, 227, 478, 608, 650
TfiI GAWTC 6 cut(s) 32, 44, 415, 503, 512, 655
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 1 cut(s) 279
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 4 cut(s) 568, 571, 574, 639
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 4 cut(s) 23, 407, 472, 774
TspRI CASTG 1 cut(s) 279
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 218, 263
XapI RAATTY 3 cut(s) 76, 155, 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.