Rmu_sc0004591.1_g000007

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004591.1
OG0023072
Physical Location & Seq
Reverse (-)
21654 .. 24653
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004591.1_g000007.1.cds

Sequence Viewer

Length: 564 bp
atgttggtcatacgggacataaaactcggtgtacagccggatccaaccctggaagccagagtagaatcagtttccggtgatagagaaggagaggttgaaggccaggcaaggagtgagaactattgggctgacggaagcagatgtggtggatccggagatgggcgtagggattgtggtggtgatcggtggtggggatggaggttacaggatgtttacctagagagtgattgccaggttgccattcaggaggttggtgcggatgattttgagttgagtgaaaatgtaaacatcctttcttatatccgaaatagcagtaaacatgttaaggacttgtcttttatgtttgctcctagaactgccaacatgatggctcatagactagctgcttttgcttatgaatccaatgccaatcagtctcgagctcctcccaaactcgagcgcctctctcctcactcgaactcaccgctcttgttacctacatcgcctccttcatctacctcctcggcttcatctacctcctccgaccccgaccccaacttcctcctgctttctccgccctcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

187

Amino Acids

20.49

Weight (kDa)

4.87

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023072)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001556.1_g000011 Rmu_sc0004591.1_g000007 Rmu_sc0010224.1_g000004
rosa_roxburghii Rroxscaffold_2G00087670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 466
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 3 cut(s) 257, 464, 554
AclWI GGATC 4 cut(s) 35, 48, 144, 157
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 159
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 1 cut(s) 98
AjnI CCWGG 3 cut(s) 48, 102, 231
AluBI AGCT 2 cut(s) 383, 422
AluI AGCT 2 cut(s) 383, 422
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 1 cut(s) 420
AlwI GGATC 4 cut(s) 35, 48, 144, 157
Ama87I CYCGRG 2 cut(s) 417, 434
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 383
AspLEI GCGC 1 cut(s) 441
AsuHPI GGTGA 3 cut(s) 89, 191, 453
AvaI CYCGRG 2 cut(s) 417, 434
BamHI GGATCC 2 cut(s) 40, 149
BanII GRGCYC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 1 cut(s) 370
BccI CCATC 3 cut(s) 152, 189, 361
BciT130I CCWGG 3 cut(s) 50, 104, 233
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 4 cut(s) 218, 351, 380, 562
BfoI RGCGCY 1 cut(s) 442
BisI GCNGC 1 cut(s) 384
BlsI GCNGC 1 cut(s) 385
Bme1390I CCNGG 3 cut(s) 50, 104, 233
BmeT110I CYCGRG 2 cut(s) 417, 434
BmiI GGNNCC 2 cut(s) 42, 151
BmrFI CCNGG 3 cut(s) 50, 104, 233
BplI GAGNNNNNCTC 2 cut(s) 428, 460
BsaJI CCNNGG 2 cut(s) 48, 501
BsaWI WCCGGW 2 cut(s) 74, 152
BsaXI ACNNNNNCTCC 2 cut(s) 469, 499
Bsc4I CCNNNNNNNGG 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 3 cut(s) 50, 104, 233
BseDI CCNNGG 2 cut(s) 48, 501
BseGI GGATG 4 cut(s) 200, 214, 265, 288
BseLI CCNNNNNNNGG 1 cut(s) 159
BseRI GAGGAG 4 cut(s) 414, 438, 490, 508
BseXI GCAGC 1 cut(s) 370
BshFI GGCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 424
BsiHKCI CYCGRG 2 cut(s) 417, 434
BsiSI CCGG 3 cut(s) 38, 75, 153
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 1 cut(s) 102
BsoBI CYCGRG 2 cut(s) 417, 434
Bsp1286I GDGCHC 1 cut(s) 424
Bsp13I TCCGGA 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 31
Bsp143I GATC 3 cut(s) 40, 149, 181
BspACI CCGC 3 cut(s) 257, 464, 554
BspANI GGCC 1 cut(s) 102
BspEI TCCGGA 1 cut(s) 152
BspLI GGNNCC 2 cut(s) 42, 151
BspPI GGATC 4 cut(s) 35, 48, 144, 157
BsrBI CCGCTC 1 cut(s) 466
BsrGI TGTACA 1 cut(s) 31
BssECI CCNNGG 2 cut(s) 48, 501
BssMI GATC 3 cut(s) 40, 149, 181
Bst2UI CCWGG 3 cut(s) 50, 104, 233
BstAUI TGTACA 1 cut(s) 31
BstF5I GGATG 4 cut(s) 200, 214, 265, 288
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 3 cut(s) 43, 152, 184
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 3 cut(s) 40, 149, 181
BstMWI GCNNNNNNNGC 2 cut(s) 389, 553
BstNI CCWGG 3 cut(s) 50, 104, 233
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 3 cut(s) 48, 102, 231
BstV1I GCAGC 1 cut(s) 370
BstX2I RGATCY 2 cut(s) 40, 149
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 2 cut(s) 40, 149
BsuRI GGCC 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 465
BtsCI GGATG 4 cut(s) 200, 214, 265, 288
CfoI GCGC 1 cut(s) 441
Csp6I GTAC 1 cut(s) 32
CviAII CATG 2 cut(s) 320, 364
CviJI RGCY 8 cut(s) 37, 56, 102, 128, 371, 383, 422, 506
CviKI_1 RGCY 8 cut(s) 37, 56, 102, 128, 371, 383, 422, 506
CviQI GTAC 1 cut(s) 32
DpnI GATC 3 cut(s) 42, 151, 183
DpnII GATC 3 cut(s) 40, 149, 181
EciI GGCGGA 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 422
Eco24I GRGCYC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 422
Eco88I CYCGRG 2 cut(s) 417, 434
EcoICRI GAGCTC 1 cut(s) 422
EcoRII CCWGG 3 cut(s) 48, 102, 231
EcoT38I GRGCYC 1 cut(s) 424
FaeI CATG 2 cut(s) 323, 367
FaiI YATR 8 cut(s) 11, 20, 300, 321, 341, 365, 375, 396
FaqI GGGAC 1 cut(s) 29
FatI CATG 2 cut(s) 319, 363
Fnu4HI GCNGC 1 cut(s) 384
FokI GGATG 4 cut(s) 207, 221, 272, 275
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 1 cut(s) 384
FspBI CTAG 4 cut(s) 218, 351, 380, 562
GlaI GCGC 1 cut(s) 440
GluI GCNGC 1 cut(s) 384
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 1 cut(s) 102
HapII CCGG 3 cut(s) 38, 75, 153
HhaI GCGC 1 cut(s) 441
Hin1II CATG 2 cut(s) 323, 367
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HinfI GANTC 2 cut(s) 65, 398
HpaII CCGG 3 cut(s) 38, 75, 153
HphI GGTGA 3 cut(s) 89, 191, 453
Hpy166II GTNNAC 4 cut(s) 32, 214, 286, 317
Hpy188I TCNGA 2 cut(s) 305, 523
Hpy188III TCNNGA 3 cut(s) 153, 245, 417
Hpy8I GTNNAC 4 cut(s) 32, 214, 286, 317
HpyAV CCTTC 3 cut(s) 80, 92, 498
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 553
Hsp92II CATG 2 cut(s) 323, 367
HspAI GCGC 1 cut(s) 439
Kpn2I TCCGGA 1 cut(s) 152
Kzo9I GATC 3 cut(s) 40, 149, 181
LmnI GCTCC 2 cut(s) 352, 427
Lsp1109I GCAGC 1 cut(s) 370
MaeI CTAG 4 cut(s) 218, 351, 380, 562
MaeIII GTNAC 2 cut(s) 201, 471
MalI GATC 3 cut(s) 42, 151, 183
MbiI CCGCTC 1 cut(s) 466
MboI GATC 3 cut(s) 40, 149, 181
MflI RGATCY 2 cut(s) 40, 149
MhlI GDGCHC 1 cut(s) 424
MmeI TCCRAC 2 cut(s) 68, 546
MroI TCCGGA 1 cut(s) 152
MseI TTAA 1 cut(s) 324
MspI CCGG 3 cut(s) 38, 75, 153
MspR9I CCNGG 3 cut(s) 50, 104, 233
MvaI CCWGG 3 cut(s) 50, 104, 233
MwoI GCNNNNNNNGC 2 cut(s) 389, 553
NdeII GATC 3 cut(s) 40, 149, 181
NlaIII CATG 2 cut(s) 323, 367
NlaIV GGNNCC 2 cut(s) 42, 151
NmeAIII GCCGAG 1 cut(s) 482
NspI RCATGY 1 cut(s) 323
PaeR7I CTCGAG 2 cut(s) 417, 434
PciI ACATGT 1 cut(s) 319
PfeI GAWTC 2 cut(s) 65, 398
PkrI GCNGC 1 cut(s) 385
PscI ACATGT 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 424
Psp6I CCWGG 3 cut(s) 48, 102, 231
PspGI CCWGG 3 cut(s) 48, 102, 231
PspN4I GGNNCC 2 cut(s) 42, 151
PspXI VCTCGAGB 1 cut(s) 434
PsuI RGATCY 2 cut(s) 40, 149
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
SacI GAGCTC 1 cut(s) 424
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 1 cut(s) 384
Sau3AI GATC 3 cut(s) 40, 149, 181
ScrFI CCNGG 3 cut(s) 50, 104, 233
SduI GDGCHC 1 cut(s) 424
Sfr274I CTCGAG 2 cut(s) 417, 434
SlaI CTCGAG 2 cut(s) 417, 434
SmlI CTYRAG 2 cut(s) 417, 434
SmoI CTYRAG 2 cut(s) 417, 434
SsiI CCGC 3 cut(s) 257, 464, 554
SspMI CTAG 4 cut(s) 218, 351, 380, 562
SstI GAGCTC 1 cut(s) 424
StyD4I CCNGG 3 cut(s) 48, 102, 231
TaqI TCGA 3 cut(s) 418, 435, 455
TatI WGTACW 1 cut(s) 31
TfiI GAWTC 2 cut(s) 65, 398
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TseI GCWGC 1 cut(s) 383
TspDTI ATGAA 3 cut(s) 411, 480, 498
TspGWI ACGGA 1 cut(s) 147
XceI RCATGY 1 cut(s) 323
XhoI CTCGAG 2 cut(s) 417, 434
XspI CTAG 4 cut(s) 218, 351, 380, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.