Rmu_sc0004948.1_g000002

CRAL-TRIO domain-containing protein C23B6.04c-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004948.1
OG0020043
Physical Location & Seq
Reverse (-)
1849 .. 3485
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
atgtacgttgtcaatttcatgatacaaatcctggatttcaactttgatttggagacttttgtgctgtctattgtgatagagaggaccaaattagaaactggaaatgggggtacgcagagagagaagtcatcctccaccttactgtgctccggtgaccagtggcctgtggcgaccggtgaccggattccggcgaccggtgaccggattccggcgaccgttgaccggattccagaaaccggtaaccggaatccagcgaccggtcaagaacagatgtcatggttgattgacttcactggcttctcactaaacaccaatgtttccgtcaagacagctcgtgaatgtatcaacattctacagaaccattaccccgagaggcttacagttgcatttctttacaacccacctaggatttttcaggcattttggaagacgcacgtacgcccactcgtcgtcgtcgtttcatccaacacctctcctgcacaatcccaactcagccgatcggccaagacccagaagctctacgacatagttgagctcccagcaccctccggccacgcctccaacgccgccggttcagtgttcacgcacctctcggccatcatgttcgatgcccgatcatctctccggtctgcaatctcagccatggcgctcgaacccatcgccatgatcgtcgattctagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

227

Amino Acids

25.03

Weight (kDa)

6.91

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 86 - 144 2.9e-14 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020043)

Species Orthologous Gene IDs
rosa_laevigata RLG00000033650
rosa_multiflora Rmu_sc0002354.1_g000054 Rmu_sc0004948.1_g000002 Rmu_sc0012562.1_g000005
rosa_samantha Rh2AG196700 Rh2DG204600
rosa_wichuraiana Rw1G000800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 567
AcoI YGGCCR 3 cut(s) 501, 550, 594
AfaI GTAC 3 cut(s) 5, 112, 438
AfiI CCNNNNNNNGG 9 cut(s) 180, 187, 194, 201, 208, 222, 236, 243, 257
AgeI ACCGGT 4 cut(s) 173, 194, 236, 257
AgsI TTSAA 1 cut(s) 40
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 3 cut(s) 332, 517, 535
AluI AGCT 3 cut(s) 332, 517, 535
Alw21I GWGCWC 2 cut(s) 149, 537
Alw26I GTCTC 1 cut(s) 47
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 4 cut(s) 161, 501, 550, 594
AsiGI ACCGGT 4 cut(s) 173, 194, 236, 257
AspA2I CCTAGG 1 cut(s) 404
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 164, 188, 209
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 84
AvrII CCTAGG 1 cut(s) 404
BanII GRGCYC 1 cut(s) 537
BauI CACGAG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 2 cut(s) 149, 537
BccI CCATC 2 cut(s) 605, 665
BciT130I CCWGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 2 cut(s) 405, 678
BfmI CTRYAG 1 cut(s) 353
BfoI RGCGCY 1 cut(s) 650
BisI GCNGC 1 cut(s) 567
BlnI CCTAGG 1 cut(s) 404
BlsI GCNGC 1 cut(s) 568
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 84
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 434
BsaAI YACGTR 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 404, 642
Bsc4I CCNNNNNNNGG 9 cut(s) 180, 187, 194, 201, 208, 222, 236, 243, 257
Bse118I RCCGGY 5 cut(s) 173, 194, 236, 257, 569
Bse1I ACTGG 3 cut(s) 103, 157, 298
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 404, 642
BseGI GGATG 2 cut(s) 128, 461
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 9 cut(s) 180, 187, 194, 201, 208, 222, 236, 243, 257
BseMII CTCAG 2 cut(s) 505, 651
BseNI ACTGG 3 cut(s) 103, 157, 298
BseYI CCCAGC 1 cut(s) 538
BsgI GTGCAG 1 cut(s) 462
Bsh1285I CGRYCG 5 cut(s) 174, 195, 216, 258, 500
BshFI GGCC 4 cut(s) 163, 503, 552, 596
BshTI ACCGGT 4 cut(s) 173, 194, 236, 257
BsiEI CGRYCG 5 cut(s) 174, 195, 216, 258, 500
BsiHKAI GWGCWC 2 cut(s) 149, 537
BsiHKCI CYCGRG 1 cut(s) 368
BsiWI CGTACG 1 cut(s) 436
BslI CCNNNNNNNGG 9 cut(s) 180, 187, 194, 201, 208, 222, 236, 243, 257
BsmAI GTCTC 1 cut(s) 47
BsnI GGCC 4 cut(s) 163, 503, 552, 596
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 149, 537
Bsp143I GATC 3 cut(s) 497, 614, 666
Bsp19I CCATGG 1 cut(s) 642
BspACI CCGC 1 cut(s) 567
BspANI GGCC 4 cut(s) 163, 503, 552, 596
BspCNI CTCAG 2 cut(s) 504, 650
BspHI TCATGA 1 cut(s) 18
BsrFI RCCGGY 5 cut(s) 173, 194, 236, 257, 569
BsrI ACTGG 3 cut(s) 103, 157, 298
BssAI RCCGGY 5 cut(s) 173, 194, 236, 257, 569
BssECI CCNNGG 2 cut(s) 404, 642
BssMI GATC 3 cut(s) 497, 614, 666
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 404, 642
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 3 cut(s) 144, 217, 382
BstBAI YACGTR 1 cut(s) 436
BstDEI CTNAG 2 cut(s) 491, 637
BstDSI CCRYGG 1 cut(s) 642
BstEII GGTNACC 4 cut(s) 152, 176, 197, 239
BstF5I GGATG 2 cut(s) 128, 461
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 3 cut(s) 500, 617, 669
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 3 cut(s) 497, 614, 666
BstMCI CGRYCG 5 cut(s) 174, 195, 216, 258, 500
BstMWI GCNNNNNNNGC 2 cut(s) 563, 638
BstNI CCWGG 1 cut(s) 32
BstPI GGTNACC 4 cut(s) 152, 176, 197, 239
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 353
BstV2I GAAGAC 1 cut(s) 434
BsuRI GGCC 4 cut(s) 163, 503, 552, 596
BtgI CCRYGG 1 cut(s) 642
BtgZI GCGATG 1 cut(s) 643
BtsCI GGATG 2 cut(s) 128, 461
BtsIMutI CAGTG 3 cut(s) 164, 291, 582
CciI TCATGA 1 cut(s) 18
CfoI GCGC 1 cut(s) 649
Cfr10I RCCGGY 5 cut(s) 173, 194, 236, 257, 569
Cfr13I GGNCC 1 cut(s) 84
CseI GACGC 1 cut(s) 439
Csp6I GTAC 3 cut(s) 4, 111, 437
CspAI ACCGGT 4 cut(s) 173, 194, 236, 257
CviAII CATG 5 cut(s) 19, 276, 601, 643, 664
CviQI GTAC 3 cut(s) 4, 111, 437
DdeI CTNAG 2 cut(s) 491, 637
DpnI GATC 3 cut(s) 499, 616, 668
DpnII GATC 3 cut(s) 497, 614, 666
EaeI YGGCCR 3 cut(s) 501, 550, 594
Ecl136II GAGCTC 1 cut(s) 535
Eco130I CCWWGG 2 cut(s) 404, 642
Eco24I GRGCYC 1 cut(s) 537
Eco47I GGWCC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 368
Eco91I GGTNACC 4 cut(s) 152, 176, 197, 239
EcoICRI GAGCTC 1 cut(s) 535
EcoO65I GGTNACC 4 cut(s) 152, 176, 197, 239
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 404, 642
EcoT38I GRGCYC 1 cut(s) 537
ErhI CCWWGG 2 cut(s) 404, 642
FaeI CATG 5 cut(s) 22, 279, 604, 646, 667
FaiI YATR 6 cut(s) 20, 277, 527, 602, 644, 665
FatI CATG 5 cut(s) 18, 275, 600, 642, 663
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 2 cut(s) 115, 448
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 1 cut(s) 567
FspBI CTAG 2 cut(s) 405, 678
GlaI GCGC 1 cut(s) 648
GluI GCNGC 1 cut(s) 567
GsaI CCCAGC 1 cut(s) 542
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 4 cut(s) 163, 503, 552, 596
HgaI GACGC 1 cut(s) 439
HhaI GCGC 1 cut(s) 649
Hin1II CATG 5 cut(s) 22, 279, 604, 646, 667
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 5 cut(s) 184, 205, 226, 247, 674
HphI GGTGA 3 cut(s) 164, 188, 209
Hpy166II GTNNAC 2 cut(s) 220, 582
Hpy188III TCNNGA 6 cut(s) 19, 230, 263, 325, 335, 678
Hpy8I GTNNAC 2 cut(s) 220, 582
Hpy99I CGWCG 4 cut(s) 452, 455, 458, 674
HpyCH4III ACNGT 3 cut(s) 144, 217, 382
HpyCH4IV ACGT 2 cut(s) 6, 435
HpyCH4V TGCA 3 cut(s) 386, 479, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 638
HpyF3I CTNAG 2 cut(s) 491, 637
HpySE526I ACGT 2 cut(s) 6, 435
Hsp92II CATG 5 cut(s) 22, 279, 604, 646, 667
HspAI GCGC 1 cut(s) 647
Kzo9I GATC 3 cut(s) 497, 614, 666
LmnI GCTCC 2 cut(s) 152, 540
LweI GCATC 1 cut(s) 598
MaeI CTAG 2 cut(s) 405, 678
MaeII ACGT 2 cut(s) 6, 435
MaeIII GTNAC 4 cut(s) 152, 176, 197, 239
MalI GATC 3 cut(s) 499, 616, 668
MboI GATC 3 cut(s) 497, 614, 666
MboII GAAGA 1 cut(s) 439
MhlI GDGCHC 2 cut(s) 149, 537
MluCI AATT 2 cut(s) 13, 89
MmeI TCCRAC 2 cut(s) 489, 585
MnlI CCTC 7 cut(s) 75, 142, 366, 481, 556, 568, 599
MslI CAYNNNNRTG 1 cut(s) 662
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 563, 638
NcoI CCATGG 1 cut(s) 642
NdeII GATC 3 cut(s) 497, 614, 666
NlaIII CATG 5 cut(s) 22, 279, 604, 646, 667
NmeAIII GCCGAG 1 cut(s) 572
NmuCI GTSAC 3 cut(s) 152, 176, 197
PagI TCATGA 1 cut(s) 18
PcsI WCGNNNNNNNCGW 2 cut(s) 453, 666
PfeI GAWTC 5 cut(s) 184, 205, 226, 247, 674
Pfl23II CGTACG 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 30
PinAI ACCGGT 4 cut(s) 173, 194, 236, 257
PkrI GCNGC 1 cut(s) 568
Ple19I CGATCG 1 cut(s) 500
Ppu21I YACGTR 1 cut(s) 436
Psp124BI GAGCTC 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 30
PspEI GGTNACC 4 cut(s) 152, 176, 197, 239
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 1 cut(s) 30
PspLI CGTACG 1 cut(s) 436
PspPI GGNCC 1 cut(s) 84
PvuI CGATCG 1 cut(s) 500
RsaI GTAC 3 cut(s) 5, 112, 438
RsaNI GTAC 3 cut(s) 4, 111, 437
RseI CAYNNNNRTG 1 cut(s) 662
SacI GAGCTC 1 cut(s) 537
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 3 cut(s) 497, 614, 666
Sau96I GGNCC 1 cut(s) 84
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 149, 537
SetI ASST 9 cut(s) 9, 140, 334, 406, 438, 473, 519, 537, 591
SfaNI GCATC 1 cut(s) 598
SfcI CTRYAG 1 cut(s) 353
SinI GGWCC 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 662
Sse9I AATT 2 cut(s) 13, 89
SsiI CCGC 1 cut(s) 567
SspMI CTAG 2 cut(s) 405, 678
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 2 cut(s) 404, 642
TaaI ACNGT 3 cut(s) 144, 217, 382
TaiI ACGT 2 cut(s) 9, 438
TaqI TCGA 3 cut(s) 606, 651, 672
TasI AATT 2 cut(s) 13, 89
TauI GCSGC 1 cut(s) 569
TfiI GAWTC 5 cut(s) 184, 205, 226, 247, 674
TscAI CASTG 3 cut(s) 164, 298, 582
TseFI GTSAC 3 cut(s) 152, 176, 197
Tsp45I GTSAC 3 cut(s) 152, 176, 197
TspDTI ATGAA 2 cut(s) 7, 450
TspGWI ACGGA 1 cut(s) 310
TspRI CASTG 3 cut(s) 164, 298, 582
VpaK11BI GGWCC 1 cut(s) 84
XbaI TCTAGA 1 cut(s) 677
XmaJI CCTAGG 1 cut(s) 404
XspI CTAG 2 cut(s) 405, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.