Rmu_sc0006252.1_g000008

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006252.1
OG0019953
Physical Location & Seq
Forward (+)
41041 .. 42383
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
atggagaccaaagatcaagagacacgtaatgaagtgattggaattggaattcgattttctccttataaaacccagcttccccaaccagctcacgtgcctttcttttcccagatacggaagcttccctttcagaggcttgttcatgaaatcactcacgatttcaagatggacctgcgttttcggacccatgtcgtgttggcgctacaaaaggtggcggaggcctatcttgttgggttgtttgaggatattaatatgtgcaccattcatgccaagcaaatccaactcaaccacgtcgaagtccagcttgtcagagaagctcaccccaaacatctcgttctcgctaatgatcaagccttttccacacctagaaaaacattgtctcaatcagagatcacagcgctaggtacaagcctttttcgcctcgattccactgtagcgcacatgagaactgtcatctccggcgagattcgggtccgatttcagggaatttggaagccgggatccccaggttgggtccactcgagtgagggttcttgtttggcccagtctctctatgtccggctagctcgaccgtggtttagtggtgtggctcagtcccgagaatctattcgcagtcgacccgtcgtgattgtgccgatgatggcttattctgcacaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

219

Amino Acids

24.94

Weight (kDa)

9.82

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 37 - 104 5.9e-14 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019953)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0252291 RchiOBHm_Chr6g0297691 RchiOBHm_Chr7g0202341
rosa_laevigata RLG00000015397
rosa_multiflora Rmu_sc0006252.1_g000008
rosa_roxburghii Rroxscaffold_4G00315650
rosa_rugosa Rorug06G0218200.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
Acc36I ACCTGC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 616
AciI CCGC 1 cut(s) 215
AclWI GGATC 2 cut(s) 495, 508
AcsI RAATTY 2 cut(s) 48, 486
AcvI CACGTG 1 cut(s) 94
AfaI GTAC 1 cut(s) 406
AfeI AGCGCT 1 cut(s) 399
AfiI CCNNNNNNNGG 5 cut(s) 114, 132, 481, 510, 511
AflIII ACRYGT 1 cut(s) 23
AgsI TTSAA 1 cut(s) 163
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 505
AleI CACNNNNGTG 1 cut(s) 522
AluBI AGCT 6 cut(s) 76, 89, 121, 304, 317, 566
AluI AGCT 6 cut(s) 76, 89, 121, 304, 317, 566
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 3 cut(s) 14, 384, 552
Alw44I GTGCAC 1 cut(s) 256
AlwI GGATC 2 cut(s) 495, 508
Ama87I CYCGRG 2 cut(s) 520, 597
Aor51HI AGCGCT 1 cut(s) 399
AoxI GGCC 2 cut(s) 219, 540
ApaLI GTGCAC 1 cut(s) 256
ApoI RAATTY 2 cut(s) 48, 486
AseI ATTAAT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 606
AspLEI GCGC 3 cut(s) 202, 400, 439
AspS9I GGNCC 5 cut(s) 169, 183, 472, 514, 541
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 1 cut(s) 311
AsuNHI GCTAGC 1 cut(s) 562
AvaI CYCGRG 2 cut(s) 520, 597
AvaII GGWCC 4 cut(s) 169, 183, 472, 514
BaeGI GKGCMC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 500
BbrPI CACGTG 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 260
BccI CCATC 2 cut(s) 160, 634
BciT130I CCWGG 1 cut(s) 507
BclI TGATCA 1 cut(s) 346
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 3 cut(s) 14, 384, 552
BfaI CTAG 3 cut(s) 366, 401, 563
BfmI CTRYAG 1 cut(s) 432
BfoI RGCGCY 2 cut(s) 203, 401
BfuAI ACCTGC 1 cut(s) 180
Bme1390I CCNGG 2 cut(s) 498, 507
Bme18I GGWCC 4 cut(s) 169, 183, 472, 514
BmeT110I CYCGRG 2 cut(s) 520, 597
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 5 cut(s) 169, 183, 472, 514, 541
BmiI GGNNCC 4 cut(s) 185, 473, 502, 515
BmrFI CCNGG 2 cut(s) 498, 507
BmrI ACTGGG 1 cut(s) 538
BmtI GCTAGC 1 cut(s) 566
BmuI ACTGGG 1 cut(s) 538
BoxI GACNNNNGTC 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 498
BsaAI YACGTR 2 cut(s) 26, 94
BsaJI CCNNGG 2 cut(s) 505, 572
Bsc4I CCNNNNNNNGG 5 cut(s) 114, 132, 481, 510, 511
Bse1I ACTGG 1 cut(s) 544
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 505, 572
BseLI CCNNNNNNNGG 5 cut(s) 114, 132, 481, 510, 511
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 1 cut(s) 544
BseSI GKGCMC 1 cut(s) 260
BseYI CCCAGC 1 cut(s) 72
BsgI GTGCAG 1 cut(s) 636
Bsh1285I CGRYCG 1 cut(s) 572
BshFI GGCC 2 cut(s) 221, 542
BsiEI CGRYCG 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 260
BsiHKCI CYCGRG 2 cut(s) 520, 597
BsiSI CCGG 3 cut(s) 459, 497, 559
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 5 cut(s) 114, 132, 481, 510, 511
BsmAI GTCTC 3 cut(s) 14, 384, 552
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 2 cut(s) 221, 542
BsoBI CYCGRG 2 cut(s) 520, 597
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 4 cut(s) 13, 346, 390, 500
BspACI CCGC 1 cut(s) 215
BspANI GGCC 2 cut(s) 221, 542
BspCNI CTCAG 1 cut(s) 604
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 4 cut(s) 185, 473, 502, 515
BspMI ACCTGC 1 cut(s) 180
BspOI GCTAGC 1 cut(s) 566
BspPI GGATC 2 cut(s) 495, 508
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 505, 572
BssMI GATC 4 cut(s) 13, 346, 390, 500
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 433, 451, 573
BstBAI YACGTR 2 cut(s) 26, 94
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 1 cut(s) 591
BstDSI CCRYGG 1 cut(s) 572
BstENI CCTNNNNNAGG 1 cut(s) 130
BstH2I RGCGCY 2 cut(s) 203, 401
BstHHI GCGC 3 cut(s) 202, 400, 439
BstKTI GATC 4 cut(s) 16, 349, 393, 503
BstMAI GTCTC 3 cut(s) 14, 384, 552
BstMBI GATC 4 cut(s) 13, 346, 390, 500
BstMCI CGRYCG 1 cut(s) 572
BstMWI GCNNNNNNNGC 2 cut(s) 417, 650
BstNI CCWGG 1 cut(s) 507
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 496, 505
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 500
BstYI RGATCY 1 cut(s) 500
BsuRI GGCC 2 cut(s) 221, 542
BtgI CCRYGG 1 cut(s) 572
BtrI CACGTC 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 564
CciI TCATGA 1 cut(s) 142
CfoI GCGC 3 cut(s) 202, 400, 439
Cfr13I GGNCC 5 cut(s) 169, 183, 472, 514, 541
Csp6I GTAC 1 cut(s) 405
CviAII CATG 4 cut(s) 143, 188, 266, 442
CviQI GTAC 1 cut(s) 405
DdeI CTNAG 1 cut(s) 591
DpnI GATC 4 cut(s) 15, 348, 392, 502
DpnII GATC 4 cut(s) 13, 346, 390, 500
EciI GGCGGA 1 cut(s) 230
Eco147I AGGCCT 1 cut(s) 221
Eco47I GGWCC 4 cut(s) 169, 183, 472, 514
Eco47III AGCGCT 1 cut(s) 399
Eco72I CACGTG 1 cut(s) 94
Eco88I CYCGRG 2 cut(s) 520, 597
EcoNI CCTNNNNNAGG 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 505
FaeI CATG 4 cut(s) 146, 191, 269, 445
FaiI YATR 7 cut(s) 66, 144, 189, 254, 267, 443, 555
FalI AAGNNNNNCTT 4 cut(s) 110, 142, 288, 320
FaqI GGGAC 1 cut(s) 580
FatI CATG 4 cut(s) 142, 187, 265, 441
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 1 cut(s) 616
FspBI CTAG 3 cut(s) 366, 401, 563
GlaI GCGC 3 cut(s) 201, 399, 438
GsaI CCCAGC 1 cut(s) 76
HaeII RGCGCY 2 cut(s) 203, 401
HaeIII GGCC 2 cut(s) 221, 542
HapII CCGG 3 cut(s) 459, 497, 559
HhaI GCGC 3 cut(s) 202, 400, 439
Hin1II CATG 4 cut(s) 146, 191, 269, 445
Hin6I GCGC 3 cut(s) 200, 398, 437
HinP1I GCGC 3 cut(s) 200, 398, 437
HincII GTYRAC 1 cut(s) 617
HindII GTYRAC 1 cut(s) 617
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 3 cut(s) 425, 466, 602
HpaII CCGG 3 cut(s) 459, 497, 559
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 3 cut(s) 258, 517, 617
Hpy188I TCNGA 5 cut(s) 132, 183, 311, 388, 476
Hpy188III TCNNGA 6 cut(s) 17, 143, 155, 163, 597, 625
Hpy8I GTNNAC 3 cut(s) 258, 517, 617
Hpy99I CGWCG 2 cut(s) 296, 626
HpyCH4III ACNGT 3 cut(s) 433, 451, 573
HpyCH4IV ACGT 3 cut(s) 25, 93, 291
HpyCH4V TGCA 2 cut(s) 258, 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 417, 650
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 3 cut(s) 25, 93, 291
Hsp92II CATG 4 cut(s) 146, 191, 269, 445
HspAI GCGC 3 cut(s) 200, 398, 437
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 4 cut(s) 13, 346, 390, 500
MaeI CTAG 3 cut(s) 366, 401, 563
MaeII ACGT 3 cut(s) 25, 93, 291
MalI GATC 4 cut(s) 15, 348, 392, 502
MboI GATC 4 cut(s) 13, 346, 390, 500
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 3 cut(s) 42, 48, 486
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 5 cut(s) 126, 211, 235, 431, 520
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 1 cut(s) 522
MspI CCGG 3 cut(s) 459, 497, 559
MspR9I CCNGG 2 cut(s) 498, 507
MvaI CCWGG 1 cut(s) 507
MwoI GCNNNNNNNGC 2 cut(s) 417, 650
NciI CCSGG 1 cut(s) 498
NdeII GATC 4 cut(s) 13, 346, 390, 500
NheI GCTAGC 1 cut(s) 562
NlaIII CATG 4 cut(s) 146, 191, 269, 445
NlaIV GGNNCC 4 cut(s) 185, 473, 502, 515
OliI CACNNNNGTG 1 cut(s) 522
PaeR7I CTCGAG 1 cut(s) 520
PagI TCATGA 1 cut(s) 142
PceI AGGCCT 1 cut(s) 221
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 3 cut(s) 425, 466, 602
PmaCI CACGTG 1 cut(s) 94
PmlI CACGTG 1 cut(s) 94
Ppu21I YACGTR 2 cut(s) 26, 94
PshAI GACNNNNGTC 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 249
PsiI TTATAA 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 505
PspCI CACGTG 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 4 cut(s) 185, 473, 502, 515
PspPI GGNCC 5 cut(s) 169, 183, 472, 514, 541
PspXI VCTCGAGB 1 cut(s) 520
PsuI RGATCY 1 cut(s) 500
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
RseI CAYNNNNRTG 1 cut(s) 522
SalI GTCGAC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 249
Sau3AI GATC 4 cut(s) 13, 346, 390, 500
Sau96I GGNCC 5 cut(s) 169, 183, 472, 514, 541
ScrFI CCNGG 2 cut(s) 498, 507
SduI GDGCHC 1 cut(s) 260
SfcI CTRYAG 1 cut(s) 432
Sfr274I CTCGAG 1 cut(s) 520
SinI GGWCC 4 cut(s) 169, 183, 472, 514
SlaI CTCGAG 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 3 cut(s) 42, 48, 486
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 1 cut(s) 215
SspMI CTAG 3 cut(s) 366, 401, 563
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 2 cut(s) 496, 505
TaaI ACNGT 3 cut(s) 433, 451, 573
TaiI ACGT 3 cut(s) 28, 96, 294
TaqI TCGA 6 cut(s) 52, 294, 423, 521, 568, 616
TasI AATT 3 cut(s) 42, 48, 486
TfiI GAWTC 3 cut(s) 425, 466, 602
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 436
TspDTI ATGAA 4 cut(s) 45, 131, 159, 254
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 1 cut(s) 436
VneI GTGCAC 1 cut(s) 256
VpaK11BI GGWCC 4 cut(s) 169, 183, 472, 514
VspI ATTAAT 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 130
XapI RAATTY 2 cut(s) 48, 486
XhoI CTCGAG 1 cut(s) 520
XmiI GTMKAC 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 3 cut(s) 366, 401, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.