Rmu_sc0006572.1_g000004

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006572.1
OG0007693
Physical Location & Seq
Forward (+)
17822 .. 27217
9396 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4422 bp
atgagagagagaggacggcaagcaaagcaagaaagcaattggattggattggaagaagaaaatatggggagtgcgattctgaacgcgatggtgtttatggcagtgattcccgcattgtgttgtgcaaaccccccaaaagatgtgatagatgtgaaaggaggcccggagtctgttgtgtgggtggttcagctttccgatctccatttcagtgtccaccaccccgacagagcaaccgacttcaccaaacttgtcgcccctgctctctccatgatcaacccttctctcgtcctcatcactggtgatctcacagacggaaaaagcaaggatttgttaacaatgaaacaaaatgaggtggagtgggtggaataccagactgtaatggaacacgttatcgaaagcagtggacttgacaagagtatcttctttgatcttagggggaatcacgataactttggtgtaccacttcttggtggtgcctttgatttcttttcaaagtatagcattaatgggcagttgggcagaactgggaatgtcaatagtattactctgcagactggtgaccggaagcatctctttgttggagttgataccactatgtctgttggtgtacgaggcccaactaatctttttgggcatccaactgatcaactattatcagaattagatatggagctctcccactgggattctcaatcaaaagatccaataaccaagatttccttcggccattttcctctttcattctcggcaacctcacattctgggaagagcctaaaagatatattcctcaatcattccatatcagcttatatatgcgggcatctgcatgcaaggttcggtaaaaacttgaagaggcaccatcagctttcatcttcgccagaatttttccagcttaatatacaccaactatcttctcagcctcctgttaattgttcgactgatgccccaacggccaatgaattctgggagtgggagatgggtgactggaggaagagccgagccatgcgaattttggccattgatagaggtcatgtttcatacgttgatgttgactttaagttaggcaccaagaaaactatcatattgcccacttttcctctggactcacagctcatgtcaacatcttcattccgtcatgagtatgaatgtcgtgctatgggccctttttcttatgaggcaattagagccctggtgttttctgtttctccacttgtgtcagttacagctatgatctatgacacaagacctggatatctcagtatggtacttgaggctcccatgataaagcttcagcataacacctccaggggagatctatatattgctccatggaattatagggcctttgaggatccatctccagatagatattggttgcaaatagaagcaactgacttcatgggaagatcaactttgagtgatttgaggccgttttctattaatggtcttagtactaaactttcatggacgtggaaagagtttatagtcatgggttgtcaatgggctgcattatattacccgatgctgtggtctgctgtatattttttactcttgattctccttattccaaaatctctcctcattttctcaaataagcattttacttacaagaataagggctttctaaatattttaggatgggtttcacaggagttttgtaggtttcccctcacatggtttggtttagtaggatacttgttttacctcatattatttccatggtttattgggcaagtgttcactgatggcaaggataaaggatacatgacctatatgggctgggtgattaaatctgtcaatgaagatggaaaacacgaatatgttggatctccagatgtaatggtggttgtccttccacacctcttgtttgtagtctttcctgctattttggtcactggggctttaactgctgaaaaacagatctatcgggaacatgttcgttcactttcaggaaagaaagaagatgatcctgatcaagaagaaaagagatctttatggtatggtaaccggggcagtaggaaatcaaatccctgtgttggagagcggtggattcgaaaagttcttctggttgtttgcttggcaatttgttggaagcattttatgaattgcagggctctcatcaaagcatacgagatgaatcccattgtcaattttccaggatatagcctcacaattccgctactgttggcttatactgtctacagaactaggagaaatgtgacgcatttcatgcagacggacttgcctttaacctacgaagtggccaaacaaaacaaccggaagaactctgctctgttgatttgccaaagtttgaagattgaagcagccaacgaaaaagggtttccgatggagatcaatagttcaatttcatgcccttcaactcctcgttggaactccgagaggccgttcctcaccggccgcttccatcaggaaaccaaaacgacgtcgcgattcgccgccgccgatatcaaggattcccatactctgggattccaagatgccattggttgttacaatgctgctgttcaggaactgatggtcatcgatgacctcttgtccgccatggttggcatccaagggcgctacatttccatcaaacgagtccgcgcaagggaggatgatttcacctttcaagttgatgcctccatggatttagctcttcaggaattggccaaacgggtgtttcctctgtgcgagagcttcatgctcatcaatcagtttgttgaatcaagatctcagttcaagaatggcttagttaatcatgcctttgctgctgcgctcagggccctcctcctagattaccaggctatggtggcacagcttgaacaccaatttcgacttggaagactctccttacaaggcttgtggtattactgccagccaatgatgggatctatgcaagcactatccactgtaatacagaaagcttcagctaataattttgtaggttctgcagttcttaaccttgtgcagagtcaggctaaggccatggctggtgatactgctgtaaggtcattgctagaaaagatggctgaatgtgctagcaatgcctacctgggtatattagagaggtgggtctatgaaggagtcattgatgatccttatggtgaatttttcattgctgagaacaaatctctgcaaaaggaaagcctcactcaagattatgatgccaagtattggaggcaacgttacagccttaaggatggaattcctagctttcttgcaaatattgctggaacaattttgatcaccggaaagtatctaaatgtcatgagagaatgcgggcaccatgttcaggtccctgtatctgaaaattcaaaattgatgagctttggatcaaatcatcgttatatcgaatgtataaaatctgcttatgactttgcaagcagcgagctcttaaatcttatcaaagaaaagtatgatctgatgggaaagctacgatctataaagcactatcttcttcttgatcagggtgactttttagttcattttatggatattgctcgggatgagcttatgaaaaagcttgatgacatttctgtggagaaactgcaatctctactagaccttgctttgcgaactacagcagctgcagcagatccctgccatgaggacttaacatgttgtgtggaaacgtcttctttgcttagaagactagggacacttaaagatcgtgaaaagagcaggaatgttcttgatgataatgatcttgaggaaccgctgagcttaactggacttgagacattttctttgaattataaggttaggtggccattgtctatagtgatctctaagaaagccctaacaaagtatcagttgattttccgctcccttttccactgtaagcatgtagaacgccaactgtgtggggcgtggcaagtgcatcagggagttcgtgccctgaacatgcgtggaagtgctatcggcagatcatctctcctctgtcggagcatgcttaaatttattaatagccttctacactatctaacctttgaggttcttgaacccaattggcatgtgatgcataataggcttcagactgcaaagagcatagatgaggttatccaacaccatgacttcttccttgacaagtgcttgagagggtgtttgcttcttttgcctgaatttctcaagaaggtggagaggctgaaatcactttgcctacaatatgcttcagcaactcaatggttgattagttcacttgtgtcagagaaacctaagcagtccaaatcacggagccgatgtcaggcactatcaataaactccagcaacacaaattttactgatgctattctcaaattcgagagggagttcaatgcagagcttcagagcctagcaccaatactgagcagcagctcccaagcagaaccgtatttgactcatctcgcacagtggattttgggcattggaaatgatcaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

1473

Amino Acids

167.45

Weight (kDa)

8.06

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 61 - 277 7.4e-13 Calcineurin-like phosphoesterase
Ig_TMM62 PF24384 356 - 483 1.1e-50 TMEM62 Ig-like domain
TMEM62_C PF24394 506 - 719 2.2e-95 TMEM62 C-terminal domain
GCP_N_terminal PF17681 848 - 1139 4.1e-78 Gamma tubulin complex component N-terminal
GCP_C_terminal PF04130 1142 - 1463 5.3e-66 Gamma tubulin complex component C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3759
AatII GACGTC 1 cut(s) 2447
AccB1I GGYRCC 4 cut(s) 473, 855, 1064, 3285
AccB7I CCANNNNNTGG 4 cut(s) 467, 2485, 2809, 3177
AccBSI CCGCTC 2 cut(s) 2044, 3828
AccI GTMKAC 1 cut(s) 2199
AccII CGCG 3 cut(s) 86, 2449, 2607
AclI AACGTT 1 cut(s) 3187
AclWI GGATC 9 cut(s) 695, 1346, 1359, 1834, 1961, 2899, 3092, 3343, 3593
AcoI YGGCCR 7 cut(s) 724, 951, 1014, 2262, 2416, 2670, 3770
AcuI CTGAAG 6 cut(s) 1274, 2645, 2913, 4019, 4158, 4310
AcyI GRCGYC 1 cut(s) 2444
AfaI GTAC 4 cut(s) 459, 609, 1266, 1453
AflII CTTAAG 1 cut(s) 3197
AflIII ACRYGT 3 cut(s) 385, 1933, 3620
AhdI GACNNNNNGTC 1 cut(s) 2554
AjiI CACGTC 1 cut(s) 1470
AjnI CCWGG 6 cut(s) 1188, 1246, 1304, 2155, 2802, 3054
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
Alw21I GWGCWC 2 cut(s) 675, 3396
Alw26I GTCTC 1 cut(s) 3734
AlwI GGATC 9 cut(s) 695, 1346, 1359, 1834, 1961, 2899, 3092, 3343, 3593
AlwNI CAGNNNCTG 2 cut(s) 2533, 3590
Ama87I CYCGRG 1 cut(s) 3504
ApaI GGGCCC 2 cut(s) 1163, 2788
ArsI GACNNNNNNTTYG 2 cut(s) 3733, 3765
AseI ATTAAT 3 cut(s) 504, 1440, 3966
Asp700I GAANNNNTTC 1 cut(s) 1935
AspLEI GCGC 3 cut(s) 2583, 2609, 2779
AspS9I GGNCC 8 cut(s) 161, 614, 1159, 1160, 1341, 2784, 2785, 3298
AsuC2I CCSGG 2 cut(s) 164, 2009
AsuII TTCGAA 1 cut(s) 2053
AsuNHI GCTAGC 1 cut(s) 3041
AvaI CYCGRG 1 cut(s) 3504
AvaII GGWCC 1 cut(s) 3298
BaeGI GKGCMC 4 cut(s) 1163, 2788, 3288, 3901
BaeI ACNNNNGTAYC 4 cut(s) 400, 433, 2991, 3024
BalI TGGCCA 4 cut(s) 1016, 2264, 2672, 3772
BamHI GGATCC 1 cut(s) 1351
BanI GGYRCC 4 cut(s) 473, 855, 1064, 3285
BanII GRGCYC 6 cut(s) 675, 1163, 1189, 2116, 2788, 3396
BbsI GAAGAC 3 cut(s) 2851, 3630, 3658
Bbv12I GWGCWC 2 cut(s) 675, 3396
BceAI ACGGC 4 cut(s) 32, 966, 1414, 2389
BciT130I CCWGG 6 cut(s) 1190, 1248, 1306, 2157, 2804, 3056
BciVI GTATCC 2 cut(s) 1685, 1754
BclI TGATCA 6 cut(s) 270, 643, 1972, 3246, 3466, 4414
BcnI CCSGG 2 cut(s) 164, 2009
BcoDI GTCTC 1 cut(s) 3734
BfaI CTAG 8 cut(s) 2208, 2795, 3020, 3042, 3213, 3563, 3656, 4334
BfmI CTRYAG 6 cut(s) 548, 2200, 2952, 3582, 3591, 3780
BfoI RGCGCY 1 cut(s) 2584
BfrI CTTAAG 1 cut(s) 3197
BfuI GTATCC 2 cut(s) 1685, 1754
BglI GCCNNNNNGGC 1 cut(s) 950
BglII AGATCT 4 cut(s) 1312, 1920, 1988, 2732
BlpI GCTNAGC 1 cut(s) 3722
BmcAI AGTACT 1 cut(s) 1453
Bme1390I CCNGG 8 cut(s) 164, 1190, 1248, 1306, 2009, 2157, 2804, 3056
Bme18I GGWCC 1 cut(s) 3298
BmeRI GACNNNNNGTC 1 cut(s) 2554
BmeT110I CYCGRG 1 cut(s) 3504
BmgBI CACGTC 1 cut(s) 1470
BmgT120I GGNCC 8 cut(s) 161, 614, 1159, 1160, 1341, 2784, 2785, 3298
BmrFI CCNGG 8 cut(s) 164, 1190, 1248, 1306, 2009, 2157, 2804, 3056
BmrI ACTGGG 3 cut(s) 534, 691, 1905
BmtI GCTAGC 1 cut(s) 3045
BmuI ACTGGG 3 cut(s) 534, 691, 1905
BpiI GAAGAC 3 cut(s) 2851, 3630, 3658
BpmI CTGGAG 5 cut(s) 1006, 1288, 1344, 1815, 4249
Bpu10I CCTNAGC 3 cut(s) 2780, 2982, 4218
Bpu1102I GCTNAGC 1 cut(s) 3722
Bpu14I TTCGAA 1 cut(s) 2053
BpuEI CTTGAG 6 cut(s) 1289, 3141, 3731, 3758, 4115, 4117
BpuMI CCSGG 2 cut(s) 164, 2009
Bsa29I ATCGAT 1 cut(s) 2544
BsaBI GATNNNNATC 4 cut(s) 1971, 2351, 2540, 3705
BsaHI GRCGYC 1 cut(s) 2444
BsaWI WCCGGW 3 cut(s) 561, 2277, 3251
BsaXI ACNNNNNCTCC 4 cut(s) 3172, 3202, 4228, 4258
Bse118I RCCGGY 1 cut(s) 2414
Bse1I ACTGG 7 cut(s) 301, 529, 559, 686, 989, 1900, 3736
Bse3DI GCAATG 3 cut(s) 3014, 3052, 3117
Bse8I GATNNNNATC 4 cut(s) 1971, 2351, 2540, 3705
BseBI CCWGG 6 cut(s) 1190, 1248, 1306, 2157, 2804, 3056
BseCI ATCGAT 1 cut(s) 2544
BseGI GGATG 6 cut(s) 634, 1643, 2571, 2623, 3208, 3514
BseJI GATNNNNATC 4 cut(s) 1971, 2351, 2540, 3705
BseMI GCAATG 3 cut(s) 3014, 3052, 3117
BseMII CTCAG 7 cut(s) 929, 1270, 2750, 2794, 3114, 3713, 4337
BseNI ACTGG 7 cut(s) 301, 529, 559, 686, 989, 1900, 3736
BseRI GAGGAG 4 cut(s) 1568, 2373, 2780, 3929
BseSI GKGCMC 4 cut(s) 1163, 2788, 3288, 3901
BseX3I CGGCCG 1 cut(s) 2416
BseYI CCCAGC 1 cut(s) 1779
BsgI GTGCAG 1 cut(s) 2990
Bsh1236I CGCG 3 cut(s) 86, 2449, 2607
Bsh1285I CGRYCG 1 cut(s) 2419
BshNI GGYRCC 4 cut(s) 473, 855, 1064, 3285
BshVI ATCGAT 1 cut(s) 2544
BsiEI CGRYCG 1 cut(s) 2419
BsiHKAI GWGCWC 2 cut(s) 675, 3396
BsiHKCI CYCGRG 1 cut(s) 3504
BsiSI CCGG 6 cut(s) 164, 562, 2008, 2278, 2415, 3252
BslFI GGGAC 2 cut(s) 3284, 3673
BsmAI GTCTC 1 cut(s) 3734
BsmFI GGGAC 2 cut(s) 3284, 3673
BsmI GAATGC 1 cut(s) 3284
BsoBI CYCGRG 1 cut(s) 3504
Bsp119I TTCGAA 1 cut(s) 2053
Bsp120I GGGCCC 2 cut(s) 1159, 2784
Bsp1286I GDGCHC 8 cut(s) 675, 1163, 1189, 2116, 2788, 3288, 3396, 3901
Bsp1720I GCTNAGC 1 cut(s) 3722
Bsp19I CCATGG 5 cut(s) 1328, 1718, 2562, 2646, 2988
Bsp68I TCGCGA 1 cut(s) 2449
BspCNI CTCAG 7 cut(s) 928, 1269, 2749, 2793, 3115, 3714, 4338
BspDI ATCGAT 1 cut(s) 2544
BspFNI CGCG 3 cut(s) 86, 2449, 2607
BspHI TCATGA 2 cut(s) 1135, 3270
BspMAI CTGCAG 3 cut(s) 552, 2956, 3595
BspOI GCTAGC 1 cut(s) 3045
BspPI GGATC 9 cut(s) 695, 1346, 1359, 1834, 1961, 2899, 3092, 3343, 3593
BspQI GCTCTTC 3 cut(s) 761, 986, 2664
BspT104I TTCGAA 1 cut(s) 2053
BspT107I GGYRCC 4 cut(s) 473, 855, 1064, 3285
BspTI CTTAAG 1 cut(s) 3197
BsrBI CCGCTC 2 cut(s) 2044, 3828
BsrDI GCAATG 3 cut(s) 3014, 3052, 3117
BsrFI RCCGGY 1 cut(s) 2414
BsrI ACTGG 7 cut(s) 301, 529, 559, 686, 989, 1900, 3736
BssAI RCCGGY 1 cut(s) 2414
BssNI GRCGYC 1 cut(s) 2444
BssT1I CCWWGG 6 cut(s) 1328, 1718, 2562, 2575, 2646, 2988
Bst2UI CCWGG 6 cut(s) 1190, 1248, 1306, 2157, 2804, 3056
Bst4CI ACNGT 8 cut(s) 376, 2184, 2197, 2914, 3842, 3864, 4371, 4392
Bst6I CTCTTC 4 cut(s) 761, 845, 986, 2664
BstACI GRCGYC 1 cut(s) 2444
BstAFI CTTAAG 1 cut(s) 3197
BstAPI GCANNNNNTGC 3 cut(s) 2230, 3230, 4021
BstBI TTCGAA 1 cut(s) 2053
BstDSI CCRYGG 5 cut(s) 1328, 1718, 2562, 2646, 2988
BstEII GGTNACC 2 cut(s) 557, 2003
BstF5I GGATG 6 cut(s) 634, 1643, 2571, 2623, 3208, 3514
BstFNI CGCG 3 cut(s) 86, 2449, 2607
BstH2I RGCGCY 1 cut(s) 2584
BstHHI GCGC 3 cut(s) 2583, 2609, 2779
BstMAI GTCTC 1 cut(s) 3734
BstMCI CGRYCG 1 cut(s) 2419
BstNI CCWGG 6 cut(s) 1190, 1248, 1306, 2157, 2804, 3056
BstNSI RCATGY 7 cut(s) 830, 1937, 3624, 3851, 3910, 3955, 4019
BstPI GGTNACC 2 cut(s) 557, 2003
BstSCI CCNGG 8 cut(s) 162, 1188, 1246, 1304, 2007, 2155, 2802, 3054
BstSFI CTRYAG 6 cut(s) 548, 2200, 2952, 3582, 3591, 3780
BstSLI GKGCMC 4 cut(s) 1163, 2788, 3288, 3901
BstUI CGCG 3 cut(s) 86, 2449, 2607
BstV2I GAAGAC 3 cut(s) 2851, 3630, 3658
BstX2I RGATCY 9 cut(s) 700, 1312, 1351, 1826, 1920, 1988, 2732, 2891, 3598
BstXI CCANNNNNNTGG 1 cut(s) 3866
BstYI RGATCY 9 cut(s) 700, 1312, 1351, 1826, 1920, 1988, 2732, 2891, 3598
BstZI CGGCCG 1 cut(s) 2416
Bsu15I ATCGAT 1 cut(s) 2544
BsuI GTATCC 2 cut(s) 1685, 1754
BsuTUI ATCGAT 1 cut(s) 2544
BtgI CCRYGG 5 cut(s) 1328, 1718, 2562, 2646, 2988
BtgZI GCGATG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 1470
BtsCI GGATG 6 cut(s) 634, 1643, 2571, 2623, 3208, 3514
BtsI GCAGTG 2 cut(s) 108, 406
BtuMI TCGCGA 1 cut(s) 2449
CaiI CAGNNNCTG 2 cut(s) 2533, 3590
CciI TCATGA 2 cut(s) 1135, 3270
CfoI GCGC 3 cut(s) 2583, 2609, 2779
Cfr10I RCCGGY 1 cut(s) 2414
Cfr13I GGNCC 8 cut(s) 161, 614, 1159, 1160, 1341, 2784, 2785, 3298
ClaI ATCGAT 1 cut(s) 2544
CseI GACGC 1 cut(s) 2230
Csp6I GTAC 4 cut(s) 458, 608, 1265, 1452
CspCI CAANNNNNGTGG 4 cut(s) 1845, 1880, 2846, 2881
CviQI GTAC 4 cut(s) 458, 608, 1265, 1452
DriI GACNNNNNGTC 1 cut(s) 2554
EaeI YGGCCR 7 cut(s) 724, 951, 1014, 2262, 2416, 2670, 3770
EagI CGGCCG 1 cut(s) 2416
Eam1104I CTCTTC 4 cut(s) 761, 845, 986, 2664
Eam1105I GACNNNNNGTC 1 cut(s) 2554
EarI CTCTTC 4 cut(s) 761, 845, 986, 2664
EciI GGCGGA 1 cut(s) 2548
Ecl136II GAGCTC 2 cut(s) 673, 3394
EclXI CGGCCG 1 cut(s) 2416
Eco130I CCWWGG 6 cut(s) 1328, 1718, 2562, 2575, 2646, 2988
Eco24I GRGCYC 6 cut(s) 675, 1163, 1189, 2116, 2788, 3396
Eco32I GATATC 2 cut(s) 1253, 2467
Eco47I GGWCC 1 cut(s) 3298
Eco52I CGGCCG 1 cut(s) 2416
Eco53kI GAGCTC 2 cut(s) 673, 3394
Eco57I CTGAAG 6 cut(s) 1274, 2645, 2913, 4019, 4158, 4310
Eco88I CYCGRG 1 cut(s) 3504
Eco91I GGTNACC 2 cut(s) 557, 2003
EcoICRI GAGCTC 2 cut(s) 673, 3394
EcoO109I RGGNCCY 5 cut(s) 1160, 1341, 2784, 2785, 3298
EcoO65I GGTNACC 2 cut(s) 557, 2003
EcoRI GAATTC 2 cut(s) 959, 3207
EcoRII CCWGG 6 cut(s) 1188, 1246, 1304, 2155, 2802, 3054
EcoRV GATATC 2 cut(s) 1253, 2467
EcoT14I CCWWGG 6 cut(s) 1328, 1718, 2562, 2575, 2646, 2988
EcoT22I ATGCAT 1 cut(s) 4026
EcoT38I GRGCYC 6 cut(s) 675, 1163, 1189, 2116, 2788, 3396
ErhI CCWWGG 6 cut(s) 1328, 1718, 2562, 2575, 2646, 2988
FaqI GGGAC 2 cut(s) 3284, 3673
FauI CCCGC 3 cut(s) 118, 809, 3275
FbaI TGATCA 6 cut(s) 270, 643, 1972, 3246, 3466, 4414
FblI GTMKAC 1 cut(s) 2199
FokI GGATG 6 cut(s) 621, 1650, 2558, 2630, 3215, 3521
FriOI GRGCYC 6 cut(s) 675, 1163, 1189, 2116, 2788, 3396
FspBI CTAG 8 cut(s) 2208, 2795, 3020, 3042, 3213, 3563, 3656, 4334
GlaI GCGC 3 cut(s) 2582, 2608, 2778
GsaI CCCAGC 1 cut(s) 1783
GsuI CTGGAG 5 cut(s) 1006, 1288, 1344, 1815, 4249
HaeII RGCGCY 1 cut(s) 2584
HapII CCGG 6 cut(s) 164, 562, 2008, 2278, 2415, 3252
HgaI GACGC 1 cut(s) 2230
HhaI GCGC 3 cut(s) 2583, 2609, 2779
Hin1I GRCGYC 1 cut(s) 2444
Hin6I GCGC 3 cut(s) 2581, 2607, 2777
HinP1I GCGC 3 cut(s) 2581, 2607, 2777
HincII GTYRAC 3 cut(s) 333, 1051, 1119
HindII GTYRAC 3 cut(s) 333, 1051, 1119
HindIII AAGCTT 3 cut(s) 1286, 2925, 3524
HpaI GTTAAC 1 cut(s) 333
HpaII CCGG 6 cut(s) 164, 562, 2008, 2278, 2415, 3252
Hpy99I CGWCG 2 cut(s) 2446, 2449
HpyAV CCTTC 7 cut(s) 288, 730, 1862, 2385, 3077, 3983, 4129
HpyCH4III ACNGT 8 cut(s) 376, 2184, 2197, 2914, 3842, 3864, 4371, 4392
HpyCH4IV ACGT 6 cut(s) 387, 1041, 1469, 2444, 3187, 3635
HpySE526I ACGT 6 cut(s) 387, 1041, 1469, 2444, 3187, 3635
Hsp92I GRCGYC 1 cut(s) 2444
HspAI GCGC 3 cut(s) 2581, 2607, 2777
Ksp22I TGATCA 6 cut(s) 270, 643, 1972, 3246, 3466, 4414
KspAI GTTAAC 1 cut(s) 333
LguI GCTCTTC 3 cut(s) 761, 986, 2664
LmnI GCTCC 7 cut(s) 670, 1279, 1330, 3833, 3948, 4236, 4361
MaeI CTAG 8 cut(s) 2208, 2795, 3020, 3042, 3213, 3563, 3656, 4334
MaeII ACGT 6 cut(s) 387, 1041, 1469, 2444, 3187, 3635
MaeIII GTNAC 9 cut(s) 557, 980, 1219, 1891, 2003, 2218, 2510, 3188, 3473
MbiI CCGCTC 2 cut(s) 2044, 3828
MfeI CAATTG 2 cut(s) 37, 4009
MflI RGATCY 9 cut(s) 700, 1312, 1351, 1826, 1920, 1988, 2732, 2891, 3598
MhlI GDGCHC 8 cut(s) 675, 1163, 1189, 2116, 2788, 3288, 3396, 3901
MlsI TGGCCA 4 cut(s) 1016, 2264, 2672, 3772
MluNI TGGCCA 4 cut(s) 1016, 2264, 2672, 3772
MlyI GAGTC 7 cut(s) 176, 1097, 2610, 2841, 2983, 3096, 4372
MmeI TCCRAC 8 cut(s) 559, 662, 1804, 2017, 2069, 2369, 3926, 4090
Mox20I TGGCCA 4 cut(s) 1016, 2264, 2672, 3772
Mph1103I ATGCAT 1 cut(s) 4026
MroXI GAANNNNTTC 1 cut(s) 1935
MscI TGGCCA 4 cut(s) 1016, 2264, 2672, 3772
MslI CAYNNNNRTG 6 cut(s) 207, 825, 1140, 1468, 1818, 3539
Msp20I TGGCCA 4 cut(s) 1016, 2264, 2672, 3772
MspA1I CMGCKG 2 cut(s) 3590, 3721
MspCI CTTAAG 1 cut(s) 3197
MspI CCGG 6 cut(s) 164, 562, 2008, 2278, 2415, 3252
MspR9I CCNGG 8 cut(s) 164, 1190, 1248, 1306, 2009, 2157, 2804, 3056
MunI CAATTG 2 cut(s) 37, 4009
Mva1269I GAATGC 1 cut(s) 3284
MvaI CCWGG 6 cut(s) 1190, 1248, 1306, 2157, 2804, 3056
MvnI CGCG 3 cut(s) 86, 2449, 2607
NciI CCSGG 2 cut(s) 164, 2009
NcoI CCATGG 5 cut(s) 1328, 1718, 2562, 2646, 2988
NheI GCTAGC 1 cut(s) 3041
NmeAIII GCCGAG 2 cut(s) 725, 1022
NmuCI GTSAC 5 cut(s) 557, 980, 1891, 2218, 3473
NruI TCGCGA 1 cut(s) 2449
NsiI ATGCAT 1 cut(s) 4026
NspI RCATGY 7 cut(s) 830, 1937, 3624, 3851, 3910, 3955, 4019
NspV TTCGAA 1 cut(s) 2053
PaeI GCATGC 2 cut(s) 830, 3955
PagI TCATGA 2 cut(s) 1135, 3270
PciI ACATGT 2 cut(s) 1933, 3620
PciSI GCTCTTC 3 cut(s) 761, 986, 2664
PcsI WCGNNNNNNNCGW 1 cut(s) 2460
PctI GAATGC 1 cut(s) 3284
PdmI GAANNNNTTC 1 cut(s) 1935
PflMI CCANNNNNTGG 4 cut(s) 467, 2485, 2809, 3177
PfoI TCCNGGA 1 cut(s) 2155
PleI GAGTC 7 cut(s) 175, 1097, 2609, 2841, 2982, 3095, 4372
PpsI GAGTC 7 cut(s) 175, 1097, 2609, 2841, 2982, 3095, 4372
PpuMI RGGWCCY 1 cut(s) 3298
PscI ACATGT 2 cut(s) 1933, 3620
PshBI ATTAAT 3 cut(s) 504, 1440, 3966
PsiI TTATAA 1 cut(s) 3759
Psp124BI GAGCTC 2 cut(s) 675, 3396
Psp1406I AACGTT 1 cut(s) 3187
Psp5II RGGWCCY 1 cut(s) 3298
Psp6I CCWGG 6 cut(s) 1188, 1246, 1304, 2155, 2802, 3054
PspEI GGTNACC 2 cut(s) 557, 2003
PspFI CCCAGC 1 cut(s) 1779
PspGI CCWGG 6 cut(s) 1188, 1246, 1304, 2155, 2802, 3054
PspOMI GGGCCC 2 cut(s) 1159, 2784
PspPI GGNCC 8 cut(s) 161, 614, 1159, 1160, 1341, 2784, 2785, 3298
PspPPI RGGWCCY 1 cut(s) 3298
PstI CTGCAG 3 cut(s) 552, 2956, 3595
PstNI CAGNNNCTG 2 cut(s) 2533, 3590
PsuI RGATCY 9 cut(s) 700, 1312, 1351, 1826, 1920, 1988, 2732, 2891, 3598
PvuII CAGCTG 1 cut(s) 3590
RruI TCGCGA 1 cut(s) 2449
RsaI GTAC 4 cut(s) 459, 609, 1266, 1453
RsaNI GTAC 4 cut(s) 458, 608, 1265, 1452
RseI CAYNNNNRTG 6 cut(s) 207, 825, 1140, 1468, 1818, 3539
SacI GAGCTC 2 cut(s) 675, 3396
SapI GCTCTTC 3 cut(s) 761, 986, 2664
Sau96I GGNCC 8 cut(s) 161, 614, 1159, 1160, 1341, 2784, 2785, 3298
ScaI AGTACT 1 cut(s) 1453
SchI GAGTC 7 cut(s) 176, 1097, 2610, 2841, 2983, 3096, 4372
ScrFI CCNGG 8 cut(s) 164, 1190, 1248, 1306, 2009, 2157, 2804, 3056
SduI GDGCHC 8 cut(s) 675, 1163, 1189, 2116, 2788, 3288, 3396, 3901
SfcI CTRYAG 6 cut(s) 548, 2200, 2952, 3582, 3591, 3780
SfuI TTCGAA 1 cut(s) 2053
SinI GGWCC 1 cut(s) 3298
SmiMI CAYNNNNRTG 6 cut(s) 207, 825, 1140, 1468, 1818, 3539
SmlI CTYRAG 7 cut(s) 1268, 3156, 3197, 3710, 3737, 4096, 4130
SmoI CTYRAG 7 cut(s) 1268, 3156, 3197, 3710, 3737, 4096, 4130
SphI GCATGC 2 cut(s) 830, 3955
SspI AATATT 2 cut(s) 1630, 3229
SspMI CTAG 8 cut(s) 2208, 2795, 3020, 3042, 3213, 3563, 3656, 4334
SstI GAGCTC 2 cut(s) 675, 3396
StyD4I CCNGG 8 cut(s) 162, 1188, 1246, 1304, 2007, 2155, 2802, 3054
StyI CCWWGG 6 cut(s) 1328, 1718, 2562, 2575, 2646, 2988
TaaI ACNGT 8 cut(s) 376, 2184, 2197, 2914, 3842, 3864, 4371, 4392
TaiI ACGT 6 cut(s) 390, 1044, 1472, 2447, 3190, 3638
TaqI TCGA 7 cut(s) 393, 935, 2053, 2544, 2836, 3354, 4302
TatI WGTACW 1 cut(s) 1451
TauI GCSGC 3 cut(s) 2421, 2459, 2462
TseFI GTSAC 5 cut(s) 557, 980, 1891, 2218, 3473
Tsp45I GTSAC 5 cut(s) 557, 980, 1891, 2218, 3473
TspGWI ACGGA 4 cut(s) 327, 1121, 2252, 4249
Van91I CCANNNNNTGG 4 cut(s) 467, 2485, 2809, 3177
Vha464I CTTAAG 1 cut(s) 3197
VpaK11BI GGWCC 1 cut(s) 3298
VspI ATTAAT 3 cut(s) 504, 1440, 3966
XceI RCATGY 7 cut(s) 830, 1937, 3624, 3851, 3910, 3955, 4019
XcmI CCANNNNNNNNNTGG 5 cut(s) 1009, 1096, 1368, 2501, 2837
XmiI GTMKAC 1 cut(s) 2199
XmnI GAANNNNTTC 1 cut(s) 1935
XspI CTAG 8 cut(s) 2208, 2795, 3020, 3042, 3213, 3563, 3656, 4334
ZraI GACGTC 1 cut(s) 2445
ZrmI AGTACT 1 cut(s) 1453
Zsp2I ATGCAT 1 cut(s) 4026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.