Rmu_sc0007384.1_g000004

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007384.1
OG0021261
Physical Location & Seq
Reverse (-)
10322 .. 14855
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007384.1_g000004.1.cds

Sequence Viewer

Length: 1008 bp
atgaatccaagtttatccagaatattgatgaagagatctcaattcaaagtatcaaatcggacatacatgaaagttgctaagaatcctgtaggactggaatctcgtgtccgggatatgcatgagcttctatgtgttgaggaaaatgatgttcgcatggtgggaatatggggaattggaggaattggtaagacaactgttgccaaagctgtttatgggtcaattgctcatcgatttgaaggtagctgttttttggaaaatgtgagagaaaggtcattagtgccacatgaaggcttggttcaactgcaagaaactcttctttccaagattttagggggtgtaggagtgaagctgagcaatgttgatgatcctgcttatgatatagagaaaagactttggaataagagggacggagatggtgaatcggaatctggctggggagaacgagttggtgaagaggaagaagcagtgagttggagtaggaggaaggcgaggaagaagaagcggaggtcgcagactcagagatcgccgacgacagtggccggaaataatattccaaagtggttcaatttttgtaagcatccaactgatcatgagtattgcgagtttgttattaaacttcctccaaatttcagtggaaagaattcgagattggctttatcggctgtgtttgaaagcatggatggaacactaacctatgattataatgattatggaaaacacgcgtttcatgtccgagtctacattaatggtgatgaaatattttgggttcaccagcatctacttattcgtccggggtcaaatcatgcgtggctgcagtatgtttcgttatctgatatgaggcattgggggggacggtggaatgaagagcaacttttgagcaagtgtgaagtgagatttttgtcttctaaaccactttccttgaaagcttgtggtctccaactagtatacccccgaaacgagtatggcaacattgatcaaaacttagcagatcttcaatttttctcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

335

Amino Acids

38.41

Weight (kDa)

9.0

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021261)

Species Orthologous Gene IDs
pyrus_communis pycom04g11010 pycom15g33760
rosa_chinensis RchiOBHm_Chr6g0275961
rosa_multiflora Rmu_sc0007384.1_g000004
rosa_samantha Rh3CG276200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AccI GTMKAC 2 cut(s) 738, 945
AccII CGCG 1 cut(s) 722
AciI CCGC 1 cut(s) 502
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 625, 640
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 7 cut(s) 46, 236, 299, 565, 671, 922, 995
AhlI ACTAGT 1 cut(s) 940
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
AluBI AGCT 5 cut(s) 124, 206, 243, 349, 926
AluI AGCT 5 cut(s) 124, 206, 243, 349, 926
Alw26I GTCTC 1 cut(s) 938
AlwI GGATC 1 cut(s) 359
AoxI GGCC 1 cut(s) 537
ApeKI GCWGC 1 cut(s) 811
ApoI RAATTY 2 cut(s) 625, 640
AseI ATTAAT 1 cut(s) 744
Asp700I GAANNNNTTC 2 cut(s) 312, 640
AsuC2I CCSGG 2 cut(s) 110, 792
AsuHPI GGTGA 4 cut(s) 428, 461, 761, 761
BauI CACGAG 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 894
BbvI GCAGC 1 cut(s) 798
BccI CCATC 2 cut(s) 407, 674
BclI TGATCA 2 cut(s) 586, 973
BcnI CCSGG 2 cut(s) 110, 792
BcoDI GTCTC 1 cut(s) 938
BcuI ACTAGT 1 cut(s) 940
BfaI CTAG 1 cut(s) 941
BfmI CTRYAG 2 cut(s) 87, 812
BglII AGATCT 2 cut(s) 35, 988
BisI GCNGC 1 cut(s) 812
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 1 cut(s) 813
Bme1390I CCNGG 2 cut(s) 110, 792
BmrFI CCNGG 2 cut(s) 110, 792
BmsI GCATC 2 cut(s) 586, 784
BpiI GAAGAC 1 cut(s) 894
Bpu1102I GCTNAGC 1 cut(s) 350
BpuMI CCSGG 2 cut(s) 110, 792
Bsa29I ATCGAT 1 cut(s) 229
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 1 cut(s) 791
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 361
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 1 cut(s) 791
BseGI GGATG 2 cut(s) 577, 685
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 361
BseMII CTCAG 2 cut(s) 341, 530
BseNI ACTGG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 798
BseYI CCCAGC 1 cut(s) 432
Bsh1236I CGCG 1 cut(s) 722
BshFI GGCC 1 cut(s) 539
BshVI ATCGAT 1 cut(s) 229
BsiSI CCGG 3 cut(s) 109, 540, 791
BslFI GGGAC 2 cut(s) 419, 864
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 938
BsmFI GGGAC 2 cut(s) 419, 864
BsnI GGCC 1 cut(s) 539
Bso31I GGTCTC 1 cut(s) 938
Bsp143I GATC 6 cut(s) 35, 364, 521, 586, 973, 988
Bsp1720I GCTNAGC 1 cut(s) 350
BspACI CCGC 1 cut(s) 502
BspANI GGCC 1 cut(s) 539
BspCNI CTCAG 2 cut(s) 342, 529
BspDI ATCGAT 1 cut(s) 229
BspFNI CGCG 1 cut(s) 722
BspHI TCATGA 1 cut(s) 589
BspMAI CTGCAG 1 cut(s) 816
BspPI GGATC 1 cut(s) 359
BspQI GCTCTTC 1 cut(s) 858
BspTNI GGTCTC 1 cut(s) 938
BsrDI GCAATG 1 cut(s) 361
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 791
BssMI GATC 6 cut(s) 35, 364, 521, 586, 973, 988
BssNAI GTATAC 1 cut(s) 946
BssSI CACGAG 1 cut(s) 102
Bst1107I GTATAC 1 cut(s) 946
Bst2BI CACGAG 1 cut(s) 102
Bst4CI ACNGT 3 cut(s) 196, 535, 855
Bst6I CTCTTC 4 cut(s) 26, 318, 447, 858
BstDEI CTNAG 4 cut(s) 78, 350, 516, 982
BstF5I GGATG 2 cut(s) 577, 685
BstFNI CGCG 1 cut(s) 722
BstKTI GATC 6 cut(s) 38, 367, 524, 589, 976, 991
BstMAI GTCTC 1 cut(s) 938
BstMBI GATC 6 cut(s) 35, 364, 521, 586, 973, 988
BstMWI GCNNNNNNNGC 2 cut(s) 508, 659
BstSCI CCNGG 2 cut(s) 108, 790
BstSFI CTRYAG 2 cut(s) 87, 812
BstUI CGCG 1 cut(s) 722
BstV1I GCAGC 1 cut(s) 798
BstV2I GAAGAC 1 cut(s) 894
BstX2I RGATCY 2 cut(s) 35, 988
BstYI RGATCY 2 cut(s) 35, 988
BstZ17I GTATAC 1 cut(s) 946
Bsu15I ATCGAT 1 cut(s) 229
BsuRI GGCC 1 cut(s) 539
BsuTUI ATCGAT 1 cut(s) 229
BtsCI GGATG 2 cut(s) 577, 685
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 471, 540, 637
CciI TCATGA 1 cut(s) 589
ClaI ATCGAT 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 900, 935
CviAII CATG 8 cut(s) 67, 119, 154, 284, 590, 676, 728, 803
DdeI CTNAG 4 cut(s) 78, 350, 516, 982
DpnI GATC 6 cut(s) 37, 366, 523, 588, 975, 990
DpnII GATC 6 cut(s) 35, 364, 521, 586, 973, 988
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 4 cut(s) 26, 318, 447, 858
EarI CTCTTC 4 cut(s) 26, 318, 447, 858
Eco31I GGTCTC 1 cut(s) 938
EcoRI GAATTC 1 cut(s) 640
EcoT22I ATGCAT 1 cut(s) 120
FaeI CATG 8 cut(s) 70, 122, 157, 287, 593, 679, 731, 806
FalI AAGNNNNNCTT 4 cut(s) 297, 329, 855, 887
FaqI GGGAC 2 cut(s) 419, 864
FatI CATG 8 cut(s) 66, 118, 153, 283, 589, 675, 727, 802
FbaI TGATCA 2 cut(s) 586, 973
FblI GTMKAC 2 cut(s) 738, 945
Fnu4HI GCNGC 1 cut(s) 812
FokI GGATG 2 cut(s) 564, 692
Fsp4HI GCNGC 1 cut(s) 812
FspBI CTAG 1 cut(s) 941
GluI GCNGC 1 cut(s) 812
GsaI CCCAGC 1 cut(s) 436
HaeIII GGCC 1 cut(s) 539
HapII CCGG 3 cut(s) 109, 540, 791
Hin1II CATG 8 cut(s) 70, 122, 157, 287, 593, 679, 731, 806
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 7 cut(s) 4, 82, 98, 419, 425, 514, 735
HpaII CCGG 3 cut(s) 109, 540, 791
HphI GGTGA 4 cut(s) 428, 461, 761, 761
Hpy166II GTNNAC 3 cut(s) 739, 769, 946
Hpy188I TCNGA 5 cut(s) 60, 424, 519, 734, 832
Hpy188III TCNNGA 4 cut(s) 18, 590, 645, 1005
Hpy8I GTNNAC 3 cut(s) 739, 769, 946
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 3 cut(s) 230, 281, 478
HpyCH4III ACNGT 3 cut(s) 196, 535, 855
HpyCH4V TGCA 3 cut(s) 118, 304, 814
HpyF10VI GCNNNNNNNGC 2 cut(s) 508, 659
HpyF3I CTNAG 4 cut(s) 78, 350, 516, 982
Hsp92II CATG 8 cut(s) 70, 122, 157, 287, 593, 679, 731, 806
Ksp22I TGATCA 2 cut(s) 586, 973
Kzo9I GATC 6 cut(s) 35, 364, 521, 586, 973, 988
LguI GCTCTTC 1 cut(s) 858
Lsp1109I GCAGC 1 cut(s) 798
LweI GCATC 2 cut(s) 586, 784
MaeI CTAG 1 cut(s) 941
MalI GATC 6 cut(s) 37, 366, 523, 588, 975, 990
MboI GATC 6 cut(s) 35, 364, 521, 586, 973, 988
MboII GAAGA 9 cut(s) 43, 305, 464, 470, 505, 508, 875, 894, 983
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 2 cut(s) 35, 988
MluCI AATT 8 cut(s) 41, 171, 180, 219, 565, 625, 640, 995
MluI ACGCGT 1 cut(s) 720
MlyI GAGTC 2 cut(s) 508, 744
MmeI TCCRAC 3 cut(s) 452, 605, 961
MnlI CCTC 9 cut(s) 130, 170, 396, 448, 474, 483, 498, 630, 831
Mph1103I ATGCAT 1 cut(s) 120
MroXI GAANNNNTTC 2 cut(s) 312, 640
MseI TTAA 2 cut(s) 612, 744
MspI CCGG 3 cut(s) 109, 540, 791
MspR9I CCNGG 2 cut(s) 110, 792
MunI CAATTG 1 cut(s) 219
MvnI CGCG 1 cut(s) 722
MwoI GCNNNNNNNGC 2 cut(s) 508, 659
NciI CCSGG 2 cut(s) 110, 792
NdeII GATC 6 cut(s) 35, 364, 521, 586, 973, 988
NlaIII CATG 8 cut(s) 70, 122, 157, 287, 593, 679, 731, 806
NsiI ATGCAT 1 cut(s) 120
PagI TCATGA 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 858
PdmI GAANNNNTTC 2 cut(s) 312, 640
PfeI GAWTC 5 cut(s) 4, 82, 98, 419, 425
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 1 cut(s) 813
PleI GAGTC 2 cut(s) 508, 743
PpsI GAGTC 2 cut(s) 508, 743
PshBI ATTAAT 1 cut(s) 744
PsiI TTATAA 1 cut(s) 702
PspFI CCCAGC 1 cut(s) 432
PstI CTGCAG 1 cut(s) 816
PsuI RGATCY 2 cut(s) 35, 988
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 2 cut(s) 612, 744
SatI GCNGC 1 cut(s) 812
Sau3AI GATC 6 cut(s) 35, 364, 521, 586, 973, 988
SchI GAGTC 2 cut(s) 508, 744
ScrFI CCNGG 2 cut(s) 110, 792
SetI ASST 9 cut(s) 126, 208, 241, 245, 272, 351, 509, 695, 928
SfaNI GCATC 2 cut(s) 586, 784
SfcI CTRYAG 2 cut(s) 87, 812
SpeI ACTAGT 1 cut(s) 940
Sse9I AATT 8 cut(s) 41, 171, 180, 219, 565, 625, 640, 995
SsiI CCGC 1 cut(s) 502
SspI AATATT 3 cut(s) 24, 550, 759
SspMI CTAG 1 cut(s) 941
StyD4I CCNGG 2 cut(s) 108, 790
TaaI ACNGT 3 cut(s) 196, 535, 855
TaqI TCGA 2 cut(s) 229, 644
TasI AATT 8 cut(s) 41, 171, 180, 219, 565, 625, 640, 995
TfiI GAWTC 5 cut(s) 4, 82, 98, 419, 425
Tru1I TTAA 2 cut(s) 612, 744
Tru9I TTAA 2 cut(s) 612, 744
TscAI CASTG 3 cut(s) 471, 540, 637
TseI GCWGC 1 cut(s) 811
TspDTI ATGAA 7 cut(s) 17, 44, 83, 300, 716, 768, 876
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 3 cut(s) 471, 540, 637
VspI ATTAAT 1 cut(s) 744
XapI RAATTY 2 cut(s) 625, 640
XmiI GTMKAC 2 cut(s) 738, 945
XmnI GAANNNNTTC 2 cut(s) 312, 640
XspI CTAG 1 cut(s) 941
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.