Rmu_sc0008676.1_g000025

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008676.1
OG0023037
Physical Location & Seq
Forward (+)
87551 .. 88039
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008676.1_g000025.1.cds

Sequence Viewer

Length: 489 bp
atggcaggtgattttaatgaaatcatgtgccaggctgacaagtccggcggagtaccacgagctattgcacccatggcttcatttagacatacgatggtggattgtggcctcgttgatatgggattcagtggcagtaggcatacttggtccaataagtttaccaaagaacctcttgacagaagttttcaatcaattcaatggagggattcttacccttacagtaggacaatcacctgccaccaaatgagtctgatcattaccccatccttgttgaactgcgatctgagcaacatcatttcaggaaagctagcaggagatttaggtttgaagaaatgtggcatgacaatgaacaatgcaatgccatcattcaaaacaactggtcagcccctacaaccggcaacatgctgcatcaaattggacagaaaatttctaacacaggtacacaactcttacgctggcataccacagaatttgagaagcaaaaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

162

Amino Acids

17.98

Weight (kDa)

9.32

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023037)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000355.1_g000026 Rmu_sc0004574.1_g000006 Rmu_sc0008676.1_g000025 Rmu_sc0021503.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 242
AciI CCGC 1 cut(s) 48
AcsI RAATTY 2 cut(s) 425, 469
AfaI GTAC 2 cut(s) 54, 441
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 5 cut(s) 188, 197, 274, 328, 370
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 2 cut(s) 62, 307
AluI AGCT 2 cut(s) 62, 307
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 405
ApoI RAATTY 2 cut(s) 425, 469
AspS9I GGNCC 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 20, 223
AsuNHI GCTAGC 1 cut(s) 307
AvaII GGWCC 1 cut(s) 147
BauI CACGAG 1 cut(s) 57
BbvI GCAGC 1 cut(s) 392
BccI CCATC 3 cut(s) 88, 271, 370
BciT130I CCWGG 1 cut(s) 32
BclI TGATCA 1 cut(s) 252
BfaI CTAG 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 242
BisI GCNGC 1 cut(s) 406
BlsI GCNGC 1 cut(s) 407
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 417
BmtI GCTAGC 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 363
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 1 cut(s) 382
BseXI GCAGC 1 cut(s) 392
BshFI GGCC 1 cut(s) 108
BsiSI CCGG 2 cut(s) 45, 395
BslI CCNNNNNNNGG 1 cut(s) 394
BsnI GGCC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 252, 280
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 1 cut(s) 48
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 1 cut(s) 276
BspMI ACCTGC 1 cut(s) 242
BspOI GCTAGC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 252, 280
BssSI CACGAG 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 309, 457
BstDEI CTNAG 1 cut(s) 284
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 263
BstKTI GATC 2 cut(s) 255, 283
BstMBI GATC 2 cut(s) 252, 280
BstMWI GCNNNNNNNGC 2 cut(s) 74, 285
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 405
BstSCI CCNGG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 392
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 263
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 1 cut(s) 242
Cac8I GCNNGC 2 cut(s) 309, 457
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 53, 440
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 4 cut(s) 25, 73, 340, 402
CviJI RGCY 6 cut(s) 35, 62, 77, 108, 307, 385
CviKI_1 RGCY 6 cut(s) 35, 62, 77, 108, 307, 385
CviQI GTAC 2 cut(s) 53, 440
DdeI CTNAG 1 cut(s) 284
DpnI GATC 2 cut(s) 254, 282
DpnII GATC 2 cut(s) 252, 280
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 28, 76, 343, 405
FaiI YATR 8 cut(s) 26, 74, 90, 119, 141, 341, 403, 461
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 4 cut(s) 24, 72, 339, 401
FbaI TGATCA 1 cut(s) 252
Fnu4HI GCNGC 1 cut(s) 406
FokI GGATG 1 cut(s) 250
Fsp4HI GCNGC 1 cut(s) 406
FspBI CTAG 1 cut(s) 308
GluI GCNGC 1 cut(s) 406
HaeIII GGCC 1 cut(s) 108
HapII CCGG 2 cut(s) 45, 395
Hin1II CATG 4 cut(s) 28, 76, 343, 405
HinfI GANTC 3 cut(s) 123, 206, 247
HpaII CCGG 2 cut(s) 45, 395
HphI GGTGA 2 cut(s) 20, 223
Hpy166II GTNNAC 2 cut(s) 159, 442
Hpy188I TCNGA 2 cut(s) 252, 285
Hpy188III TCNNGA 2 cut(s) 173, 300
Hpy8I GTNNAC 2 cut(s) 159, 442
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 3 cut(s) 68, 356, 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 285
HpyF3I CTNAG 1 cut(s) 284
Hsp92II CATG 4 cut(s) 28, 76, 343, 405
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 2 cut(s) 252, 280
Lsp1109I GCAGC 1 cut(s) 392
LweI GCATC 1 cut(s) 417
MaeI CTAG 1 cut(s) 308
MalI GATC 2 cut(s) 254, 282
MboI GATC 2 cut(s) 252, 280
MboII GAAGA 1 cut(s) 340
MluCI AATT 4 cut(s) 192, 413, 425, 469
MlyI GAGTC 1 cut(s) 256
MnlI CCTC 3 cut(s) 119, 180, 195
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 344
MspI CCGG 2 cut(s) 45, 395
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 74, 285
NcoI CCATGG 1 cut(s) 72
NdeII GATC 2 cut(s) 252, 280
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 4 cut(s) 28, 76, 343, 405
NspI RCATGY 1 cut(s) 405
PaqCI CACCTGC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 123, 206
PkrI GCNGC 1 cut(s) 407
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspPI GGNCC 1 cut(s) 147
RsaI GTAC 2 cut(s) 54, 441
RsaNI GTAC 2 cut(s) 53, 440
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 1 cut(s) 406
Sau3AI GATC 2 cut(s) 252, 280
Sau96I GGNCC 1 cut(s) 147
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 32
SetI ASST 8 cut(s) 10, 64, 172, 236, 309, 325, 441, 488
SfaNI GCATC 1 cut(s) 417
SinI GGWCC 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 4 cut(s) 192, 413, 425, 469
SsiI CCGC 1 cut(s) 48
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 221
TasI AATT 4 cut(s) 192, 413, 425, 469
TfiI GAWTC 2 cut(s) 123, 206
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 133
TseI GCWGC 1 cut(s) 405
TspDTI ATGAA 3 cut(s) 33, 69, 362
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 147
XapI RAATTY 2 cut(s) 425, 469
XceI RCATGY 1 cut(s) 405
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.