Rmu_sc0027894.1_g000001

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027894.1
OG0020567
Physical Location & Seq
Forward (+)
1392 .. 2741
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027894.1_g000001.1.cds

Sequence Viewer

Length: 570 bp
atgttctccttaggcagggtgttcattaaggtcaagatcatgcttgactgggatcccaatggcaagcaaggacccgtgactcctctacctgatcttgttacaatccaccaaccaaaggatgatgaattgatgagtttgtcaggcgggcaccagaaattgaagcagcacctattttggtcccaactgatattgagaggaggaggttggaaggtgaaagatgagggagagatatcaagaggttgtggcagagtaaggggctgtcgtggtggtggctgcggctctgggttattcacagctggaccatattcgttggagaaaacagggacatgtaactatggtacagtgctgaagcatccttctggctgtgccaagattgtacatgttagtggcaaaggatggggctggtttgccacctttctaataatagtcttgtgcctttttggagcgtatctgttggctggtgcagtttatcgatattacagtcttggagttcctggaatagatgtatgtcttctaactcaattgatctgcttcttccatgcagccagcagacggtttagttatacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

189

Amino Acids

20.67

Weight (kDa)

9.35

Isoelectric Point (pI)

21.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020567)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0276121
rosa_multiflora Rmu_sc0027894.1_g000001
rosa_rugosa Rorug05G0489800
rosa_samantha Rh1CG318200 Rh2BG423500 Rh6AG118200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 147
AciI CCGC 2 cut(s) 144, 276
AclWI GGATC 2 cut(s) 47, 60
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 2 cut(s) 340, 378
AfiI CCNNNNNNNGG 3 cut(s) 15, 115, 552
AflIII ACRYGT 2 cut(s) 326, 379
AgsI TTSAA 1 cut(s) 160
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
AlwI GGATC 2 cut(s) 47, 60
ApeKI GCWGC 3 cut(s) 163, 273, 542
AspS9I GGNCC 3 cut(s) 71, 177, 299
AsuHPI GGTGA 1 cut(s) 223
AvaII GGWCC 3 cut(s) 71, 177, 299
AxyI CCTNAGG 1 cut(s) 10
BaeGI GKGCMC 1 cut(s) 150
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 503
BbvI GCAGC 3 cut(s) 175, 260, 554
BccI CCATC 1 cut(s) 390
BciT130I CCWGG 1 cut(s) 495
BisI GCNGC 4 cut(s) 164, 274, 277, 543
BlsI GCNGC 4 cut(s) 165, 275, 278, 544
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 3 cut(s) 71, 177, 299
BmgT120I GGNCC 3 cut(s) 71, 177, 299
BmiI GGNNCC 4 cut(s) 54, 73, 149, 179
BmrFI CCNGG 1 cut(s) 495
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 1 cut(s) 361
BmuI ACTGGG 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 472
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 115, 552
Bse1I ACTGG 1 cut(s) 53
Bse21I CCTNAGG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 495
BseCI ATCGAT 1 cut(s) 472
BseGI GGATG 3 cut(s) 124, 352, 401
BseLI CCNNNNNNNGG 3 cut(s) 15, 115, 552
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 3 cut(s) 72, 210, 213
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 3 cut(s) 175, 260, 554
BsgI GTGCAG 1 cut(s) 483
BshNI GGYRCC 1 cut(s) 147
BshVI ATCGAT 1 cut(s) 472
BslFI GGGAC 2 cut(s) 163, 337
BslI CCNNNNNNNGG 3 cut(s) 15, 115, 552
BsmFI GGGAC 2 cut(s) 163, 337
Bsp1286I GDGCHC 1 cut(s) 150
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 4 cut(s) 36, 52, 91, 525
BspACI CCGC 2 cut(s) 144, 276
BspDI ATCGAT 1 cut(s) 472
BspLI GGNNCC 4 cut(s) 54, 73, 149, 179
BspPI GGATC 2 cut(s) 47, 60
BspT107I GGYRCC 1 cut(s) 147
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 1 cut(s) 53
BssMI GATC 4 cut(s) 36, 52, 91, 525
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 3 cut(s) 343, 482, 555
BstAUI TGTACA 1 cut(s) 376
BstC8I GCNNGC 3 cut(s) 65, 146, 547
BstDEI CTNAG 1 cut(s) 10
BstENI CCTNNNNNAGG 1 cut(s) 13
BstF5I GGATG 3 cut(s) 124, 352, 401
BstKTI GATC 4 cut(s) 39, 55, 94, 528
BstMBI GATC 4 cut(s) 36, 52, 91, 525
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 2 cut(s) 330, 383
BstSCI CCNGG 1 cut(s) 493
BstSLI GKGCMC 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 175, 260, 554
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 472
Bsu36I CCTNAGG 1 cut(s) 10
BsuTUI ATCGAT 1 cut(s) 472
BtsCI GGATG 3 cut(s) 124, 352, 401
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 65, 146, 547
Cfr13I GGNCC 3 cut(s) 71, 177, 299
ClaI ATCGAT 1 cut(s) 472
Csp6I GTAC 2 cut(s) 339, 377
CviAII CATG 4 cut(s) 40, 327, 380, 539
CviJI RGCY 8 cut(s) 258, 273, 279, 296, 363, 402, 458, 545
CviKI_1 RGCY 8 cut(s) 258, 273, 279, 296, 363, 402, 458, 545
CviQI GTAC 2 cut(s) 339, 377
DdeI CTNAG 1 cut(s) 10
DpnI GATC 4 cut(s) 38, 54, 93, 527
DpnII GATC 4 cut(s) 36, 52, 91, 525
Eco32I GATATC 1 cut(s) 231
Eco47I GGWCC 3 cut(s) 71, 177, 299
Eco57I CTGAAG 1 cut(s) 368
Eco81I CCTNAGG 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 493
EcoRV GATATC 1 cut(s) 231
FaeI CATG 4 cut(s) 43, 330, 383, 542
FaiI YATR 8 cut(s) 41, 304, 328, 336, 381, 508, 540, 564
FaqI GGGAC 2 cut(s) 163, 337
FatI CATG 4 cut(s) 39, 326, 379, 538
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 4 cut(s) 164, 274, 277, 543
FokI GGATG 3 cut(s) 131, 339, 408
Fsp4HI GCNGC 4 cut(s) 164, 274, 277, 543
GluI GCNGC 4 cut(s) 164, 274, 277, 543
Hin1II CATG 4 cut(s) 43, 330, 383, 542
HinfI GANTC 1 cut(s) 79
HphI GGTGA 1 cut(s) 223
Hpy188III TCNNGA 2 cut(s) 34, 234
HpyAV CCTTC 2 cut(s) 202, 366
HpyCH4III ACNGT 3 cut(s) 343, 482, 555
HpyCH4V TGCA 2 cut(s) 464, 542
HpyF3I CTNAG 1 cut(s) 10
Hsp92II CATG 4 cut(s) 43, 330, 383, 542
Kzo9I GATC 4 cut(s) 36, 52, 91, 525
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 3 cut(s) 175, 260, 554
LweI GCATC 1 cut(s) 361
MaeIII GTNAC 3 cut(s) 76, 97, 329
MalI GATC 4 cut(s) 38, 54, 93, 527
MboI GATC 4 cut(s) 36, 52, 91, 525
MboII GAAGA 2 cut(s) 503, 526
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 3 cut(s) 125, 155, 521
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 2 cut(s) 185, 291
MnlI CCTC 6 cut(s) 93, 188, 191, 194, 214, 230
MseI TTAA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 495
MunI CAATTG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 495
NdeII GATC 4 cut(s) 36, 52, 91, 525
NlaIII CATG 4 cut(s) 43, 330, 383, 542
NlaIV GGNNCC 4 cut(s) 54, 73, 149, 179
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 2 cut(s) 330, 383
PciI ACATGT 2 cut(s) 326, 379
PfoI TCCNGGA 1 cut(s) 493
PkrI GCNGC 4 cut(s) 165, 275, 278, 544
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 71
PscI ACATGT 2 cut(s) 326, 379
Psp5II RGGWCCY 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 4 cut(s) 54, 73, 149, 179
PspPI GGNCC 3 cut(s) 71, 177, 299
PspPPI RGGWCCY 1 cut(s) 71
PsuI RGATCY 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 2 cut(s) 340, 378
RsaNI GTAC 2 cut(s) 339, 377
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 4 cut(s) 164, 274, 277, 543
Sau3AI GATC 4 cut(s) 36, 52, 91, 525
Sau96I GGNCC 3 cut(s) 71, 177, 299
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 1 cut(s) 150
SetI ASST 8 cut(s) 33, 91, 171, 205, 213, 241, 298, 416
SfaNI GCATC 1 cut(s) 361
SinI GGWCC 3 cut(s) 71, 177, 299
SmiMI CAYNNNNRTG 1 cut(s) 384
Sse9I AATT 3 cut(s) 125, 155, 521
SsiI CCGC 2 cut(s) 144, 276
StyD4I CCNGG 1 cut(s) 493
TaaI ACNGT 3 cut(s) 343, 482, 555
TaqI TCGA 1 cut(s) 472
TasI AATT 3 cut(s) 125, 155, 521
TatI WGTACW 1 cut(s) 376
TauI GCSGC 1 cut(s) 279
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 3 cut(s) 163, 273, 542
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 13, 138
TspRI CASTG 1 cut(s) 348
VpaK11BI GGWCC 3 cut(s) 71, 177, 299
XagI CCTNNNNNAGG 1 cut(s) 13
XceI RCATGY 2 cut(s) 330, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.