Rmu_sc0042607.1_g000001

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0042607.1
OG0019814
Physical Location & Seq
Forward (+)
31 .. 1037
1007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0042607.1_g000001.1.cds

Sequence Viewer

Length: 657 bp
atgttgatggggacactaacaaggtgccagaatatgaagaaatggggttcgatgtcgagatatcaaatgatggtgatgattctgatagaatgcagtgttcatgatacaaaagataagggagaggatactggaccttttagggtcaagggaaagaagagattcttgggttggctggagtttaatgcaaagacaaatacgaagaatcccatgtttgcgcttggaatgattttccccaactctgatgtcttcaagcatgctgttcacgagtcaaggtcttactttgatgcgttcctgaaatgtgatgttatgttgaacaatcacagtgagtccttcaataagacttataagagcatccttgatgctaggaaaaagccaataatactatgtttggaggaaataaggtcagtagagttaagcgaatggagatttgaattgcatggtagaggtgtaggctgtcaaactggaaagaaatttatggagacatatgaggtgatgctgcaaccaattgctgtagctcttgagtgggaagttatagagagatacattgcaccaccactgcacagaaggcaaccaggaaggtccaggacaaaggaaccggtgaagagccaccagctcttggaattgaaaaattgccaaggagccaatatattcaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

218

Amino Acids

25.4

Weight (kDa)

9.44

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AccB1I GGYRCC 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 616
AcsI RAATTY 1 cut(s) 470
AfiI CCNNNNNNNGG 1 cut(s) 616
AgeI ACCGGT 1 cut(s) 595
AgsI TTSAA 6 cut(s) 250, 313, 334, 431, 625, 652
AjnI CCWGG 2 cut(s) 571, 581
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 2 cut(s) 515, 613
AluI AGCT 2 cut(s) 515, 613
Alw26I GTCTC 1 cut(s) 473
ApeKI GCWGC 1 cut(s) 496
ApoI RAATTY 1 cut(s) 470
AsiGI ACCGGT 1 cut(s) 595
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 131, 579
AsuHPI GGTGA 3 cut(s) 85, 502, 610
AvaII GGWCC 2 cut(s) 131, 579
BanI GGYRCC 1 cut(s) 24
BauI CACGAG 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 483
BccI CCATC 1 cut(s) 64
BciT130I CCWGG 2 cut(s) 573, 583
BciVI GTATCC 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 510
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 1 cut(s) 497
BlsI GCNGC 1 cut(s) 498
Bme1390I CCNGG 2 cut(s) 573, 583
Bme18I GGWCC 2 cut(s) 131, 579
BmgT120I GGNCC 2 cut(s) 131, 579
BmiI GGNNCC 3 cut(s) 26, 594, 640
BmrFI CCNGG 2 cut(s) 573, 583
BmsI GCATC 4 cut(s) 274, 349, 360, 483
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 539
BsaJI CCNNGG 1 cut(s) 634
BsaWI WCCGGW 1 cut(s) 595
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 2 cut(s) 133, 466
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 2 cut(s) 573, 583
BseDI CCNNGG 1 cut(s) 634
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMI GCAATG 1 cut(s) 543
BseNI ACTGG 2 cut(s) 133, 466
BseXI GCAGC 1 cut(s) 483
BsgI GTGCAG 1 cut(s) 542
BshNI GGYRCC 1 cut(s) 24
BshTI ACCGGT 1 cut(s) 595
BsiSI CCGG 1 cut(s) 596
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 1 cut(s) 473
BsmFI GGGAC 1 cut(s) 25
BsmI GAATGC 1 cut(s) 95
BspHI TCATGA 1 cut(s) 100
BspLI GGNNCC 3 cut(s) 26, 594, 640
BspQI GCTCTTC 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 595
BsrI ACTGG 2 cut(s) 133, 466
BssAI RCCGGY 1 cut(s) 595
BssECI CCNNGG 1 cut(s) 634
BssSI CACGAG 1 cut(s) 263
BssT1I CCWWGG 1 cut(s) 634
Bst2BI CACGAG 1 cut(s) 263
Bst2UI CCWGG 2 cut(s) 573, 583
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 149, 596
BstC8I GCNNGC 1 cut(s) 255
BstF5I GGATG 1 cut(s) 351
BstHHI GCGC 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 473
BstMWI GCNNNNNNNGC 1 cut(s) 565
BstNI CCWGG 2 cut(s) 573, 583
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 2 cut(s) 571, 581
BstSFI CTRYAG 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 483
BstV2I GAAGAC 1 cut(s) 238
BsuI GTATCC 1 cut(s) 118
BtsCI GGATG 1 cut(s) 351
BtsI GCAGTG 2 cut(s) 100, 554
BtsIMutI CAGTG 3 cut(s) 100, 328, 554
Cac8I GCNNGC 1 cut(s) 255
CciI TCATGA 1 cut(s) 100
CfoI GCGC 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 2 cut(s) 131, 579
CspAI ACCGGT 1 cut(s) 595
CviAII CATG 4 cut(s) 101, 208, 254, 437
CviJI RGCY 7 cut(s) 172, 373, 453, 515, 606, 613, 641
CviKI_1 RGCY 7 cut(s) 172, 373, 453, 515, 606, 613, 641
Eam1104I CTCTTC 2 cut(s) 149, 596
EarI CTCTTC 2 cut(s) 149, 596
Eco130I CCWWGG 1 cut(s) 634
Eco32I GATATC 1 cut(s) 62
Eco47I GGWCC 2 cut(s) 131, 579
EcoRII CCWGG 2 cut(s) 571, 581
EcoRV GATATC 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 634
FaeI CATG 4 cut(s) 104, 211, 257, 440
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 1 cut(s) 25
FatI CATG 4 cut(s) 100, 207, 253, 436
FauNDI CATATG 1 cut(s) 484
Fnu4HI GCNGC 1 cut(s) 497
FokI GGATG 1 cut(s) 338
Fsp4HI GCNGC 1 cut(s) 497
FspBI CTAG 1 cut(s) 363
GlaI GCGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 497
GsuI CTGGAG 1 cut(s) 194
HapII CCGG 1 cut(s) 596
HhaI GCGC 1 cut(s) 217
Hin1II CATG 4 cut(s) 104, 211, 257, 440
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HinfI GANTC 5 cut(s) 79, 159, 202, 266, 326
HpaII CCGG 1 cut(s) 596
HphI GGTGA 3 cut(s) 85, 502, 610
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 84, 241
Hpy188III TCNNGA 5 cut(s) 57, 101, 263, 292, 518
Hpy8I GTNNAC 1 cut(s) 262
HpyAV CCTTC 3 cut(s) 340, 558, 570
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4V TGCA 6 cut(s) 93, 185, 436, 499, 548, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 565
Hsp92II CATG 4 cut(s) 104, 211, 257, 440
HspAI GCGC 1 cut(s) 215
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 1 cut(s) 638
Lsp1109I GCAGC 1 cut(s) 483
LweI GCATC 4 cut(s) 274, 349, 360, 483
MaeI CTAG 1 cut(s) 363
MboII GAAGA 5 cut(s) 49, 166, 211, 238, 613
MfeI CAATTG 1 cut(s) 504
MluCI AATT 5 cut(s) 431, 470, 504, 620, 628
MlyI GAGTC 2 cut(s) 275, 335
MnlI CCTC 4 cut(s) 115, 385, 437, 481
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 2 cut(s) 180, 413
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 2 cut(s) 573, 583
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 2 cut(s) 573, 583
MwoI GCNNNNNNNGC 1 cut(s) 565
NdeI CATATG 1 cut(s) 484
NlaIII CATG 4 cut(s) 104, 211, 257, 440
NlaIV GGNNCC 3 cut(s) 26, 594, 640
NspI RCATGY 1 cut(s) 257
PaeI GCATGC 1 cut(s) 257
PagI TCATGA 1 cut(s) 100
PciSI GCTCTTC 1 cut(s) 596
PctI GAATGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 3 cut(s) 79, 159, 202
PflMI CCANNNNNTGG 1 cut(s) 616
PfoI TCCNGGA 1 cut(s) 581
PinAI ACCGGT 1 cut(s) 595
PkrI GCNGC 1 cut(s) 498
PleI GAGTC 2 cut(s) 274, 334
PpsI GAGTC 2 cut(s) 274, 334
PsiI TTATAA 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 571, 581
PspGI CCWGG 2 cut(s) 571, 581
PspN4I GGNNCC 3 cut(s) 26, 594, 640
PspPI GGNCC 2 cut(s) 131, 579
SapI GCTCTTC 1 cut(s) 596
SaqAI TTAA 2 cut(s) 180, 413
SatI GCNGC 1 cut(s) 497
Sau96I GGNCC 2 cut(s) 131, 579
SchI GAGTC 2 cut(s) 275, 335
ScrFI CCNGG 2 cut(s) 573, 583
SetI ASST 9 cut(s) 26, 136, 275, 404, 448, 492, 517, 581, 615
SfaNI GCATC 4 cut(s) 274, 349, 360, 483
SfcI CTRYAG 1 cut(s) 510
SinI GGWCC 2 cut(s) 131, 579
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SphI GCATGC 1 cut(s) 257
Sse9I AATT 5 cut(s) 431, 470, 504, 620, 628
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 571, 581
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 1 cut(s) 323
TaqI TCGA 2 cut(s) 50, 56
TasI AATT 5 cut(s) 431, 470, 504, 620, 628
TfiI GAWTC 3 cut(s) 79, 159, 202
Tru1I TTAA 2 cut(s) 180, 413
Tru9I TTAA 2 cut(s) 180, 413
TscAI CASTG 3 cut(s) 100, 328, 561
TseI GCWGC 1 cut(s) 496
TspDTI ATGAA 2 cut(s) 50, 89
TspRI CASTG 3 cut(s) 100, 328, 561
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 2 cut(s) 131, 579
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.